Effect of Estrogen on Musculoskeletal Performance and Injury Risk.

Estrogen has a dramatic effect on musculoskeletal function. Beyond the known relationship between estrogen and bone, it directly affects the structure and function of other musculoskeletal tissues such as muscle, tendon, and ligament. In these other musculoskeletal tissues, estrogen improves muscle mass and strength, and increases the collagen content of connective tissues. However, unlike bone and muscle where estrogen improves function, in tendons and ligaments estrogen decreases stiffness, and this directly affects performance and injury rates. High estrogen levels can decrease power and performance and make women more prone for catastrophic ligament injury. The goal of the current work is to review the research that forms the basis of our understanding how estrogen affects muscle, tendon, and ligament and how hormonal manipulation can be used to optimize performance and promote female participation in an active lifestyle at any age

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model.

In vitro experiments are essential to understand biological mechanisms; however, the gap between monolayer tissue culture and human physiology is large, and translation of findings is often poor. Thus, there is ample opportunity for alternative experimental approaches. Here we present an approach in which human cells are isolated from human anterior cruciate ligament tissue remnants, expanded in culture, and used to form engineered ligaments. Exercise alters the biochemical milieu in the blood such that the function of many tissues, organs and bodily processes are improved. In this experiment, ligament construct culture media was supplemented with experimental human serum that has been 'conditioned' by exercise. Thus the intervention is more biologically relevant since an experimental tissue is exposed to the full endogenous biochemical milieu, including binding proteins and adjunct compounds that may be altered in tandem with the activity of an unknown agent of interest. After treatment, engineered ligaments can be analyzed for mechanical function, collagen content, morphology, and cellular biochemistry. Overall, there are four major advantages versus traditional monolayer culture and animal models, of the physiological model of ligament tissue that is presented here. First, ligament constructs are three-dimensional, allowing for mechanical properties (i.e., function) such as ultimate tensile stress, maximal tensile load, and modulus, to be quantified. Second, the enthesis, the interface between boney and sinew elements, can be examined in detail and within functional context. Third, preparing media with post-exercise serum allows for the effects of the exercise-induced biochemical milieu, which is responsible for the wide range of health benefits of exercise, to be investigated in an unbiased manner. Finally, this experimental model advances scientific research in a humane and ethical manner by replacing the use of animals, a core mandate of the National Institutes of Health, the Center for Disease Control, and the Food and Drug Administration

Correctly defined concrete syntax

Due to their complexity, the syntax of modern modeling languages is preferably defined in two steps. The abstract syntax identifies all modeling concepts whereas the concrete syntax should clarify how these concepts are rendered by graphical and/or textual elements. While the abstract syntax is often defined in form of a metamodel, there does not exist such standard format yet for concrete syntax definitions. The diversity of definition formats—ranging from EBNF grammars to informal text—is becoming a major obstacle for advances in modeling language engineering, including the automatic generation of editors. In this paper, we propose a uniform format for concrete syntax definitions. Our approach captures both textual and graphical model representations and even allows to assign more than one rendering to the same modeling concept. Consequently, following our approach, a model can have multiple, fully equivalent representations, but—in order to avoid ambiguities when reading a model representation—two different models should always have distinguishable representations. We call a syntax definition correct, if all well-formed models are represented in a non-ambiguous way. As the main contribution of this paper, we present a rigorous analysis technique to check the correctness of concrete syntax definition

Refactoring OCL annotated UML class diagrams

Refactoring of UML class diagrams is an emerging research topic and heavily inspired by refactoring of program code written in object-oriented implementation languages. Current class diagram refactoring techniques concentrate on the diagrammatic part but neglect OCL constraints that might become syntactically incorrect by changing the underlying class diagram. This paper formalizes the most important refactoring rules for class diagrams and classifies them with respect to their impact on attached OCL constraints. For refactoring rules that have an impact on OCL constraints, we formalize the necessary changes of the attached constraints. Our refactoring rules are specified in a graph-grammar inspired formalism. They have been implemented as QVT transformation rules. We finally discuss for our refactoring rules the problem of syntax preservation and show, by using the KeY-system, how this can be resolve

Ontwikkeling van plantenextracten ter bestrijding van droge mollen tot semi-commerciële producten

Droge mollen, veroorzaakt door Verticillium fungicola), vormen een belangrijk probleem in de champignonteelt. Besmetting van een teelt kan een aanzienlijke opbrengstderving tot gevolg hebben en de ziekteverwekker kan door champignonvliegen snel verspreid worden in een kwekerij. Droge mollen kunnen op dit moment nog worden bestreden door het gewasbeschermingsmiddel Sporgon. De verwachting is echter dat Sporgon binnen een termijn van een paar jaar niet meer beschikbaar zal zijn. Om die reden is onderzocht of het mogelijk is om alternatieven voor de bestrijding van droge mollen te ontwikkelen op basis van plantenextracten