Trypsin digest of cancer cells surface stimulates anti-tumor immune response better than cancer cells themselves

Antigens expressed on the surface of cancer cells are accessible targets for both humoral and cell-mediated immune responses, and are therefore potential candidates for vaccine development. Treating surface of live human breast adenocarcinoma cells (MCF-7) with trypsin yields a digest that contains 0.7% of total cell protein. Despite this difference, the trypsin digest stimulates in cytotoxicity assays anti-tumor response which kills 10-40% more cancer cells than those stimulated with cells themselves. From these results, we concluded that trypsin digest obtained from live cancer cells contains the essential antigens to induce an immune-mediated anti-tumor effect, and therefore, is candidate for anti-tumor vaccine development

Cell Proteomic Footprint

The authentication of mammalian cell cultures and their subpopulations are of tremendous demand in biotechnology and cell therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low sample cost, and high sensitivity. We discovered that cell cultures treated with protease at soft, “non-killing” conditions release fragments of cell surface proteins, which composition is a strong characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass spectrum with the mass spectrum of known cells. As an example, we applied this technique to verify subpopulations of human fibroblasts which have different origins and exhibit different medical characteristics

The horizontal model of the spatial metaphor in the media image of Russia (genres of analytical review and expert opinion)

The article was submitted on 25.05.2017.This article explores the conceptual idea of Russia’s development between 2016 and 2017 as reflected in the analytical review and expert opinion genres from online publications on socio-political issues through the horizontal model of spatial metaphors. The author analysed a total of 35 texts united by a common topic (“year in review”), with their authors having different political views. The methodological basis of the research is the idea of a metaphor as a cognitive and modelled phenomenon which plays an important role in the formation of the linguistic worldview. The purpose of the article is to establish the basic principles of how Russian reality is conceptualised within the framework of the horizontal spatial model of metaphorisation. The 766 metaphorical contexts singled out from the aforementioned texts are studied using a complex methodology of systemic contextual, semantic, and cognitive analysis of linguistic phenomena. The author establishes a general structure of this model and reveals its most frequent and relevant varieties. The author also analyses the role of conceptual metaphors in the representation of the linguistic worldview of the authors of analytical reviews and expert opinions. She argues that the model is based on the contrast between the state of rest and movement, conceived as the absence or presence of qualitative changes in the economic, socio-political, and other spheres, the desire or opportunity to change anything in those spheres coming from the representatives of the authorities, political and financial institutions, etc. It is emphasised that the figurative meanings of words characterising the state of rest and joint movement are the least frequent. This indicates that within the model in question, it is untypical of journalists and experts to present the situation in Russia during late 2016 and early 2017 as static. Additionally, the issues of interaction between different socio-political forces inside the country and those between Russia and other states are not considered priorities. The study of the metaphor is systemic and helps create a varied, but also well-structured, image of the country’s development over a year.Исследуется концептуальное представление о развитии России в 2016–2017 гг., отраженное в жанрах аналитического обзора и экспертного мнения интернет-изданий общественно-политической направленности, с помощью горизонтальной модели пространственных метафор. Анализируемые тексты опубликованы под шапкой «Итоги года», а их авторы придерживаются различных политических взглядов. В общей сложности проанализировано 35 публикаций. Методологической базой исследования стало представление о метафоре как о когнитивном и моделируемом феномене, играющем важную роль в формировании языковой картины мира. Цель – установить основные принципы концептуализации российской действительности в рамках горизонтальной пространственной модели метафоризации. Выделенные на основании сплошной выборки 766 соответствующих метафорических контекстов исследуются с применением комплексной методики системного контекстного, семантического и когнитивного анализа языковых явлений. Устанавливается общая структура модели, выявляются наиболее востребованные ее составляющие и варианты. Анализируется роль концептуальных метафор в репрезентации языковой картины мира авторов аналитических обзоров и экспертных мнений. Отмечается, что в основе исследуемой модели лежит противопоставление состояния покоя и движения, осмысляемое как отсутствие или наличие качественных изменений в экономической, социально-политической и иных сферах, желания или возможности изменить что-либо в этих сферах со стороны представителей власти, политических, финансовых институтов и т. п. Подчеркивается, что менее всего представлены переносные значения лексики, характеризующей состояние покоя и совместного движения; это свидетельствует о том, что для журналистов и экспертов (в рамках данной модели) нетипично представление о ситуации в России в 2016 – начале 2017 г. как о статичной, а проблемы взаимодействия различных социально-политических сил внутри страны и РФ с другими государствами не относятся к числу приоритетных. Исследованные метафоры системны и позволяют создать разнообразную, но четко организованную картину развития страны в течение года.Работа выполнена при финансовой поддержке Минобрнауки России в рамках базовой части государственного задания в сфере научной деятельности по проекту № 34.8128.2017/8.9

Structure-Aware Shape Synthesis

We propose a new procedure to guide training of a data-driven shape generative model using a structure-aware loss function. Complex 3D shapes often can be summarized using a coarsely defined structure which is consistent and robust across variety of observations. However, existing synthesis techniques do not account for structure during training, and thus often generate implausible and structurally unrealistic shapes. During training, we enforce structural constraints in order to enforce consistency and structure across the entire manifold. We propose a novel methodology for training 3D generative models that incorporates structural information into an end-to-end training pipeline.Comment: Accepted to 3DV 201

Journal benchmarking for strategic publication management and for improving journal positioning in the world ranking systems

Is studied Elaborating policies and strategies for the promotion of scientific journals in the world ranking systems. It is suggested to build up a journal scoreboard, which is a matrix of journal indicators, distributed for different journalsyesBelgorod State Universit

Pengaruh Penghargaan (Reward) Dan Motivasi Berprestasi Terhadap Prestasi Kerja Guru SMA Negeri Di Kabupaten Sukabumi

This study aims to determine the effect of (1) rewrad, (2) achievement motivation, (3) performance of teachers working SMAN Negeri District of SukabumI. In the data analysis, this study used a survey method using a causal analysis technique Strip . This study used a sample of 150 teachers in six high schools in District of Sukabumi are selected using Slovin formula.The results showed that : first, there are positive influence between the rewrad and performance of teachers in schools . Secondly, there is a positive effect between achievement motivation and performance of teachers in schools. Third, there is a positive effect between the reward and achievement motivatioan of teachers in schools