Diel vertical migration of the bigeye thresher shark (Alopias superciliosus), a species possessing orbital retia mirabilia

The bigeye thresher shark (Alopias superciliosus, Lowe 1841) is one of three sharks in the family Alopiidae, which occupy pelagic, neritic, and shallow coastal waters throughout the altropics and subtropics (Gruber and Compagno, 1981; Castro, 1983). All thresher sharks possess an elongated upper caudal lobe, and the bigeye thresher shark is distinguished from the other alopiid sharks by its large upward-looking eyes and grooves on the top of the head (Bigelow and Schroeder, 1948). Our present understanding of the bigeye thresher shark is primarily based upon data derived from specimens captured in fisheries, including knowledge of its morphological features (Fitch and Craig, 1964; Stillwell and Casey, 1976; Thorpe, 1997), geographic range as far as it overlaps with fisheries (Springer, 1943; Fitch and Craig, 1964; Stillwell and Casey, 1976; Gruber and Compagno, 1981; Thorpe, 1997), age, growth and maturity (Chen et al., 1997; Liu et al., 1998), and aspects of its reproductive biology (Gilmore, 1983; Moreno and Moron, 1992; Chen et al., 1997)

Skap2 is required for β2 integrin-mediated neutrophil recruitment and functions.

Integrin activation is required for neutrophil functions. Impaired integrin activation on neutrophils is the hallmark of leukocyte adhesion deficiency (LAD) syndrome in humans, characterized by impaired leukocyte recruitment and recurrent infections. The Src kinase-associated phosphoprotein 2 (Skap2) is involved in integrin functions in different leukocyte subtypes. However, the role of Skap2 in β2 integrin activation and neutrophil recruitment is unknown. In this study, we demonstrate the crucial role of Skap2 in regulating actin polymerization and binding of talin-1 and kindlin-3 to the β2 integrin cytoplasmic domain, thereby being indispensable for β2 integrin activation and neutrophil recruitment. The direct interaction of Skap2 with the Wiskott-Aldrich syndrome protein via its SH3 domain is critical for integrin activation and neutrophil recruitment in vivo. Furthermore, Skap2 regulates integrin-mediated outside-in signaling events and neutrophil functions. Thus, Skap2 is essential to activate the β2 integrins, and loss of Skap2 function is sufficient to cause a LAD-like phenotype in mice

Gut microbiota regulates K/BxN autoimmune arthritis through Tfh but not Th17 cells

The bacterial community that colonizes mucosal surfaces helps shape the development and function of the immune system. The K/BxN autoimmune arthritis model is dependent on the microbiota, and particularly on segmented filamentous bacteria, for the autoimmune phenotype. The mechanisms of how the gut microbiota affects arthritis development are not well understood. In this study, we investigate the contribution of two T cell subsets, Th17 and follicular helper T (Tfh), to arthritis and how microbiota modulates their differentiation. Using genetic approaches, we demonstrate that IL-17 is dispensable for arthritis. Antibiotic treatment inhibits disease in IL-17-deficient animals, suggesting that the gut microbiota regulates arthritis independent of Th17 cells. In contrast, conditional deletion of Bcl6 in T cells blocks Tfh cell differentiation and arthritis development. Furthermore, Tfh cell differentiation is defective in antibiotic-treated mice. Taken together, we conclude that gut microbiota regulates arthritis through Tfh but not Th17 cells. These findings have implications in our understanding of how environmental factors contribute to the development of autoimmune diseases

Kohlenstoff- und Nährelementspeicherung von Waldflächen des forstlichen Umweltmonitorings (BZE) in Rheinland-Pfalz

Im Rahmen des vorliegenden Projektes wurde an 165 repräsentativ verteilten Standorten der Bodenzustandserhebung (BZE) in Rheinland-Pfalz Biomasse- und Elementvorräte für verschiedene Waldökosystemkompartimente abgeleitet. Ziel dieser Betrachtung war es, durch die möglichst vollständige und differenzierte Erfassung, eine Aussage über die Verteilung und Höhe der Elemente im Waldökosystem zu treffen. Somit kann z.B. die Relevanz bisher wenig beachteter Kompartimente abgeschätzt werden und eine erste Aussage über die Nachhaltigkeit der forstlichen Nutzung an verschiedenen Standorten gemacht werden. Die erfassten Kompartimente beinhalten: Hauptbestand ≥8 m Oberhöhe (bzw. Bäume ≥7 cm BHD), Hauptbestand 8 m Oberhöhe bzw. Bäume ≥7 cm BHD), Wurzel des Bestandes, Bodenvegetation (Gefäßpflanzen und Bäume unter 50 cm Höhe), gesamtes oberirdisches Totholz (liegend, stehend, Wurzelstöcke), unterirdisches Totholz, Grobhumus (Fraktion 2–20 mm), Feinhumusauflage sowie Mineralboden (Wurzeltiefe und Profiltiefe). Insbesondere für die Kompartimente Verjüngung, Hauptbestand <8 m Oberhöhe, Bodenvegetation, totes Feinreisig, unterirdisches Totholz und Grobhumus lagen bisher wenig bis keine Werte vor. Die Waldökosysteme wurden somit möglichst vollständig erfasst und zusätzlich wurden auch für den Hauptbestand die Vorräte für Stammholz, Astderbholz, Reisig sowie Nadel/Blatt mit unterschiedlichen Elementgehalten und Biomassen sehr differenziert betrachtet. [...]The objective of the present study was to investigate the amount and distribution of plant biomass and nutrients of forest ecosystems in the German federal state Rhineland-Palatinate as completely and differentiated as possible. On this basis, the relevance of specific ecosystem compartments, in particular those that have previously rarely been considered, can be evaluated. Also a preliminary assessment regarding the sustainability of forestry utilization can be made. Therefore, 165 representatively distributed sites belonging to the national forest soil inventory were examined. The following compartments were considered: main forest stand (≥8 m top height / ≥7 cm diameter at breast height (DBH)), main forest stand (<8 m top height / <7 cm DBH), rejuvenation / understory under the main forest stand, roots, ground vegetation (vascular plants and trees <50 cm height), aboveground deadwood (lying, standing, rootstock), belowground deadwood, coarse humus (2–20 mm), fine humus (<2 mm) and mineral soil. The stocks of the main forest stand were further differentiated using different biomasses and nutrient contents for stem wood, merchantable branch wood (derbholz), brushwood and needle / leaves. Especially the compartments rejuvenation, ground vegetation, dead brushwood and belowground deadwood have received little to no attention in the past. [...

Quantifying mercury isotope dynamics in captive Pacific Bluefin tuna (Thunnus orientalis)

Analyses of mercury (Hg) isotope ratios in fish tissues are used increasingly to infer sources and biogeochemical processes of Hg in natural aquatic ecosystems. Controlled experiments that can couple internal Hg isotope behavior with traditional isotope tracers (delta C-13, delta N-15) can improve the applicability of Hg isotopes as natural ecological tracers. In this study, we investigated changes in Hg isotope ratios (delta Hg-202, Delta Hg-199) during bioaccumulation of natural diets in the pelagic Pacific bluefin tuna (Thunnus orientalis; PBFT). Juvenile PBFT were fed a mixture of natural prey and a dietary supplement (60% Loligo opalescens, 31% Sardinops sagax, 9% gel supplement) in captivity for 2914 days, and white muscle tissues were analyzed for Hg isotope ratios and compared to time in captivity and internal turnover of delta C-13 and delta N-15. PBFT muscle tissues equilibrated to Hg isotope ratios of the dietary mixture within similar to 700 days, after which we observed a cessation in further shifts in Delta Hg-199, and small but significant negative delta Hg-202 shifts from the dietary mixture. The internal behavior of Delta Hg-199 is consistent with previous fish studies, which showed an absence of Delta Hg-199 fractionation during Hg bioaccumulation. The negative delta Hg-202 shifts can be attributed to either preferential excretion of Hg with higher delta Hg-202 values or individual variability in captive PBFT feeding preferences and/or consumption rates. The overall internal behavior of Hg isotopes is similar to that described for delta C-13 and delta N-15, though observed Hg turnover was slower compared to carbon and nitrogen. This improved understanding of internal dynamics of Hg isotopes in relation to delta C-13 and delta N-15 enhances the applicability of Hg isotope ratios in fish tissues for tracing Hg sources in natural ecosystems.118Nsciescopu

Atlantic Bluefin Tuna (Thunnus thynnus) Diet in the Gulf of St. Lawrence and on the Eastern Scotian Shelf

The stomach contents of 68 Atlantic bluefin tuna (Thunnus thynnus) landed in Port Hood and Canso, Nova Scotia, in 2010, were analyzed to characterize the diet of bluefin tuna at the two locations. Of the sampled fish, 54 stomachs had contents. Pelagic schooling fish such as herring (Clupea harengus) and mackerel (Scomber scombrus) dominated the diets in both regions. However, a number of rare species, including demersal species, were also observed. Despite the difference in location and the significantly larger size of the Atlantic bluefin tuna landed in Port Hood, the diets of the Atlantic bluefin tuna landed at both sites were similar

Dust Production and Mass Loss in the Galactic Globular Cluster NGC 362

We investigate dust production and stellar mass loss in the Galactic globular cluster NGC 362. Due to its close proximity to the Small Magellanic Cloud (SMC), NGC 362 was imaged with the IRAC and MIPS cameras onboard the Spitzer Space Telescope as part of the Surveying the Agents of Galaxy Evolution (SAGE-SMC) Spitzer Legacy program. We detect several cluster members near the tip of the Red Giant Branch that exhibit infrared excesses indicative of circumstellar dust and find that dust is not present in measurable quantities in stars below the tip of the Red Giant Branch. We modeled the spectral energy distribution (SED) of the stars with the strongest IR excess and find a total cluster dust mass-loss rate of 3.0(+2.0/-1.2) x 10^-9 solar masses per year, corresponding to a gas mass-loss rate of 8.6(+5.6/-3.4) x 10^-6 solar masses per year, assuming [Fe/H] = -1.16. This mass loss is in addition to any dust-less mass loss that is certainly occurring within the cluster. The two most extreme stars, variables V2 and V16, contribute up to 45% of the total cluster dust-traced mass loss. The SEDs of the more moderate stars indicate the presence of silicate dust, as expected for low-mass, low-metallicity stars. Surprisingly, the SED shapes of the stars with the strongest mass-loss rates appear to require the presence of amorphous carbon dust, possibly in combination with silicate dust, despite their oxygen-rich nature. These results corroborate our previous findings in omega Centauri.Comment: 13 pages, 11 figures. Accepted to Ap

Nutritional Status Assessment During the Phase IIA and Phase III Lunar/Mars Life Support Test Project

Nutrition is a critical concern for extended-duration space missions (Smith and Lane, 1999). Loss of body weight is a primary consequence of altered nutrition, and is frequently observed during space flight (Smith and Lane; 1999). Other existing dietary concerns for space flight include excessive intakes of sodium and iron, and insufficient intakes of water and vitamin D (Smith and Lane, 1999). Furthermore, dependence on closed or semi-closed food systems increases the likelihood of inadequate intakes of key nutrients. This is a significant concern for extended-duration space missions. Space nutrition research often necessitates detailed recording of all food consumption. While this yields extremely accurate data, it requires considerable time and effort, and thus is not suitable for routine medical monitoring during space flight. To alleviate this problem, a food frequency questionnaire (FFQ) was designed to provide a quick and easy, yet reasonably accurate, method for crewmembers to provide dietary intake information to the ground. We report here a study which was designed to assess nutritional status before, during, and after the 60-d and 91-d chamber stays. An additional goal of the study was to validate a food frequency questionnaire designed specifically for use with space flight food systems

MIGRATORY MOVEMENTS OF PACIFIC BLUEFIN TUNA OFF CALIFORNIA

The genus Thunnus of the family Scombridae includes three species of bluefin tunas (Atlantic bluefin tuna – T. thynnus, Pacific bluefin tuna - T. orientalis and southern bluefin tuna - T. maccoyii). The bluefin tunas were first recognized as two independent species (Northern and Southern bluefin) based on subtle differences in morphological characters. Northern bluefin tunas are now recognized as morphologically, geographically and genetically separate species located in the Atlantic and Pacific oceans.The Pacific bluefin (T. orientalis) is the only species which remains unmanaged; this lack of management persists despite intensive fisheries on both sides of the Pacific. The current life history model indicates that these fish spawn in the western Pacific (Sea of Japan, Philippine Sea and East China Sea). Either late in the first year or early in the second year, a portion of the population migrates to the western coast of the United States and Mexico, a journey of over 8700 km (Bayliff et al., 1991). The young fish that have migrated into the eastern Pacific are thought to remain there for several years, feeding on sardines and anchovies in regions of intense upwelling (Bayliff et al., 1991, Bayliff, 1993). While these tuna are fished only seasonally off California and Mexico, they may be a year-round resident (Bayliff, 1991). The migrants then travel back to the western Pacific to spawn. Why some bluefin remain in the western Pacific while others migrate across the ocean basin is unresolved. How long they stay in the eastern Pacific, what habitats are most important, what triggers their return to the west is unclear