Beratung und Diagnostik im Schulalltag

Insgesamt soll in der geplanten Untersuchung der Frage nachgegangen werden, wie gut Lehrkräfte ihren Beratungs- und diagnostischen Aufgaben im beruflichen Kontext gerecht werden und inwieweit sie über die notwendigen Handlungskompetenzen zur Beratung und Beurteilung von Schüler*innen verfügen. Auch stellt sich die Frage, welche Rolle Fortbildungen bei der Entwicklung von Handlungskompetenzen in diesem Bereich spielen. Anhand einer empirischen Erhebung soll daher der Zusammenhang der pädagogisch-psychologischen Handlungskompetenz von Lehrkräften hinsichtlich Beratung und Diagnostik und spezifischen Lerngelegenheiten geprüft werden. Dazu wird ein Vergleich der Kompetenzen von Lehrkräften des gymnasialen und des Förderschullehramts angestrebt, da hier jeweils die größten Unterschiede zu erwarten sind. Diese Untersuchung wird im Rahmen zweier Masterarbeiten durchgeführt. Vorangegangen war in einem Projektseminar eine Pilotstudie zum Thema Beratungskompetenz bei Lehramtsstudierenden unterschiedlicher Schulformen

The RAD52-like protein ODB1 is required for the efficient excision of two mitochondrial introns spliced via first-step hydrolysis

Transcript splicing in plant mitochondria involves numerous nucleus-encoded factors, most of which are of eukaryotic origin. Some of these belong to protein families initially characterised to perform unrelated functions. The RAD52-like ODB1 protein has been reported to have roles in homologous recombination-dependent DNA repair in the nuclear and mitochondrial compartments in Arabidopsis thaliana. We show that it is additionally involved in splicing and facilitates the excision of two cis-spliced group II introns, nad1 intron 2 and nad2 intron 1, in Arabidopsis mitochondria. odb1 mutants lacking detectable amounts of ODB1 protein over-accumulated incompletely spliced nad1 and nad2 transcripts. The two ODB1-dependent introns were both found to splice via first-step hydrolysis and to be released as linear or circular molecules instead of lariats. Our systematic analysis of the structures of excised introns in Arabidopsis mitochondria revealed several other hydrolytically spliced group II introns in addition to nad1 intron 2 and nad2 intron 1, indicating that ODB1 is not a general determinant of the hydrolytic splicing pathway

Bacterial Traits Involved in Colonization of Arabidopsis thaliana Roots by Bacillus amyloliquefaciens FZB42

Colonization studies previously performed with a green-fluorescent-protein, GFP, labeled derivative of Bacillus amyloliquefaciens FZB42 revealed that the bacterium behaved different in colonizing surfaces of plant roots of different species (Fan et al., 2012). In order to extend these studies and to elucidate which genes are crucial for root colonization, we applied targeted mutant strains to Arabidopsis seedlings. The fates of root colonization in mutant strains impaired in synthesis of alternative sigma factors, non-ribosomal synthesis of lipopeptides and polyketides, biofilm formation, swarming motility, and plant growth promoting activity were analyzed by confocal laser scanning microscopy. Whilst the wild-type strain heavily colonized surfaces of root tips and lateral roots, the mutant strains were impaired in their ability to colonize root tips and most of them were unable to colonize lateral roots. Ability to colonize plant roots is not only dependent on the ability to form biofilms or swarming motility. Six mutants, deficient in abrB-, sigH-, sigD-, nrfA-, yusV and RBAM017410, but not affected in biofilm formation, displayed significantly reduced root colonization. The nrfA- and yusV-mutant strains colonized border cells and, partly, root surfaces but did not colonize root tips or lateral roots