Effects of interactions between aspergillus oryzae extract (Amaferm) and antimicrobial compounds on the growth of ruminal bacteria

The effect of Amaferm, with or without antimicrobial compounds, was determined on the growth rate of pure cultures of predominant ruminal bacteria. Adding Amaferm to media containing chlortetracyline or neomycin tended to diminish the negative effects of those compounds on the growth rate of some ruminal bacteria, even when they had shown no positive response to Amaferm alone. However, adding Amaferm to media containing tylosin decreased the growth rate of Selenomonas ruminantium D. These results indicate that Amaferm interacts both positively and negatively with certain antimicrobial compounds

Performance and ruminal microbial and metabolic development of young calves fed diets containing aspergillus oryzae extract

The 1990 Annual KSU Dairy Day is known as Dairy Day, 1990Seventy-three, neonatal, Holstein, heifer calves in one experiment and 45 neonatal, Holstein, bull calves in another were used to study the effects of dietary Aspergillus oryzae extract (Amaferm) on calf performance. Forty of the heifer calves were selected to study the effects on ruminal microbial and metabolic development. In general, Amaferm-supplemented calves had greater ruminal microbial activity than the calves fed no Amaferm. For the most part, growth and feed intake were not affected

Combined In Silico, In Vivo, and In Vitro Studies Shed Insights into the Acute Inflammatory Response in Middle-Aged Mice

We combined in silico, in vivo, and in vitro studies to gain insights into age-dependent changes in acute inflammation in response to bacterial endotoxin (LPS). Time-course cytokine, chemokine, and NO2-/NO3- data from "middle-aged" (6-8 months old) C57BL/6 mice were used to re-parameterize a mechanistic mathematical model of acute inflammation originally calibrated for "young" (2-3 months old) mice. These studies suggested that macrophages from middle-aged mice are more susceptible to cell death, as well as producing higher levels of pro-inflammatory cytokines, vs. macrophages from young mice. In support of the in silico-derived hypotheses, resident peritoneal cells from endotoxemic middle-aged mice exhibited reduced viability and produced elevated levels of TNF-α, IL-6, IL-10, and KC/CXCL1 as compared to cells from young mice. Our studies demonstrate the utility of a combined in silico, in vivo, and in vitro approach to the study of acute inflammation in shock states, and suggest hypotheses with regard to the changes in the cytokine milieu that accompany aging. © 2013 Namas et al

Evaluation of ruminal evacuation versus marker dilution procedures for estimating particulate passage rate

Call number: LD2668 .T4 ASI 1988 B44Master of ScienceAnimal Sciences and Industr

Influence of supplemental protein versus energy level on intake, fill, passage, digestibility, and fermentation characteristics of beef steers consuming dormant bluestem range forage

Two trials were conducted to evaluate effects of protein versus energy level in milo/soybean meal supplements on intake and utilization of dormant, bluestem forage. Forage dry matter intake and utilization of dormant bluestem forage appears to increase at higher levels of supplemental protein. Increased supplemental energy may be associated with depressed intake and utilization, particularly when supplements are low in protein

Anchoring Phenomena with Light and Contact Forces

OpenSciEd is a curriculum designed for the NGSS based on science storylines, phenomena-based, coherent from students’ perspective, driven by evidence, collaborative, and equitable. Instructional units for Grades 6-8 are available and will expand to include elementary through high school. Experience anchoring phenomena from Grade 6.1 Light & Matter and Grade 8.1 Contact Forces. UNI Science Education will be supporting the use of OpenSciEd with resources and future professional development

How to Integrate STEM in Your Classroom with NGSS FOSS

STEM Enrichment NGSS Modules including Variables & Design (Grades 6-8), Sound & Design (Grades 3-5), and Forces in Action (Grades K-2) are now available. Join us as we explore these STEM Enrichment NGSS Modules and discuss how they can be used in your classroom. NGSS Modules Observing Nature (Grade Pre-K) and Weather and Seasons (Grade K) will also be available to be explored and reviewed

NOD2 controls the nature of the inflammatory response and subsequent fate of Mycobacterium tuberculosis and M. bovis BCG in human macrophages

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/92022/1/j.1462-5822.2010.01544.x.pd

The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabinomannan-mediated phagosome biogenesis

Mycobacterium tuberculosis (M.tb) survives in macrophages in part by limiting phagosome–lysosome (P-L) fusion. M.tb mannose-capped lipoarabinomannan (ManLAM) blocks phagosome maturation. The pattern recognition mannose receptor (MR) binds to the ManLAM mannose caps and mediates phagocytosis of bacilli by human macrophages. Using quantitative electron and confocal microscopy, we report that engagement of the MR by ManLAM during the phagocytic process is a key step in limiting P-L fusion. P-L fusion of ManLAM microspheres was significantly reduced in human macrophages and an MR-expressing cell line but not in monocytes that lack the receptor. Moreover, reversal of P-L fusion inhibition occurred with MR blockade. Inhibition of P-L fusion did not occur with entry via Fcγ receptors or dendritic cell–specific intracellular adhesion molecule 3 grabbing nonintegrin, or with phosphatidylinositol-capped lipoarabinomannan. The ManLAM mannose cap structures were necessary in limiting P-L fusion, and the intact molecule was required to maintain this phenotype. Finally, MR blockade during phagocytosis of virulent M.tb led to a reversal of P-L fusion inhibition in human macrophages (84.0 ± 5.1% vs. 38.6 ± 0.6%). Thus, engagement of the MR by ManLAM during the phagocytic process directs M.tb to its initial phagosomal niche, thereby enhancing survival in human macrophages

Staphylococcus aureus enterotoxins induce IL-8 secretion by human nasal epithelial cells

BACKGROUND: Staphylococcus aureus produces a set of proteins which act both as superantigens and toxins. Although their mode of action as superantigens is well understood, little is known about their effects on airway epithelial cells. METHODS: To investigate this problem, primary nasal epithelial cells derived from normal and asthmatic subjects were stimulated with staphylococcal enterotoxin A and B (SEA and SEB) and secreted (supernatants) and cell-associated (cell lysates) IL-8, TNF-α, RANTES and eotaxin were determined by specific ELISAs. RESULTS: Non-toxic concentrations of SEA and SEB (0.01 μg/ml and 1.0 μg/ml) induced IL-8 secretion after 24 h of culture. Pre-treatment of the cells with IFN-γ (50 IU/ml) resulted in a further increase of IL-8 secretion. In cells from healthy donors pretreated with IFN-γ, SEA at 1.0 μg/ml induced release of 1009 pg/ml IL-8 (733.0–1216 pg/ml, median (range)) while in cells from asthmatic donors the same treatment induced significantly higher IL-8 secretion – 1550 pg/ml (1168.0–2000.0 pg/ml p = 0.04). Normal cells pre-treated with IFN-γ and then cultured with SEB at 1.0 μg/ml released 904.6 pg/ml IL-8 (666.5–1169.0 pg/ml). Cells from asthmatics treated in the same way produced significantly higher amounts of IL-8 – 1665.0 pg/ml (1168.0–2000.0 pg/ml, p = 0.01). Blocking antibodies to MHC class II molecules added to cultures stimulated with SEA and SEB, reduced IL-8 secretion by about 40% in IFN-γ unstimulated cultures and 75% in IFN-γ stimulated cultures. No secretion of TNF-α, RANTES and eotaxin was noted. CONCLUSION: Staphylococcal enterotoxins may have a role in the pathogenesis of asthma