Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis

Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+ FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 +/- 0.016) compared to the control (0.14 +/- 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 +/- 0.30 versus 6.3 +/- 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 +/- 2.22% versus 29.8 +/- 5.46%)

Administration of a Multi-Genus Synbiotic to Broilers: Effects on Gut Health, Microbial Composition and Performance

In recent years, the applicability of prebiotics, probiotics and their mixtures, defined as synbiotics, in poultry production has received considerable attention. Following the increasing regulation of antibiotic use, these nutraceuticals are seen as an alternative way to sustain production efficiency and resistance to pathogens and stressors by modulating birds’ gut health. The aim of this study was to evaluate the benefits provided under field conditions by administering the multi-species synbiotic PoultryStar® sol to broilers in drinking water. To this purpose, three Ross 308 broiler flocks, representing separate progenies of a breeder flock which was treated with the same synbiotic, were housed in separate farms, divided into treatment and control groups, and followed throughout the productive cycle. Synbiotic administration was shown to improve gut health even in absence of a challenge, with limited changes in terms of macroscopic intestinal lesions and more overt differences related to histopathological scores and villi length. Synbiotic-fed chickens performed consistently better in terms of body weight gain, feed conversion ratio and survivability. Lastly, the evaluation of the caecal microbiome through next-generation sequencing highlighted the effects of synbiotic supplementation on the composition of the bacterial population, the implications of which will, however, require further studies to be better comprehended

Administration of a Multi-Genus Synbiotic to Broilers: Effects on Gut Health, Microbial Composition and Performance

In recent years, the applicability of prebiotics, probiotics and their mixtures, defined as synbiotics, in poultry production has received considerable attention. Following the increasing regulation of antibiotic use, these nutraceuticals are seen as an alternative way to sustain production efficiency and resistance to pathogens and stressors by modulating birds’ gut health. The aim of this study was to evaluate the benefits provided under field conditions by administering the multi-species synbiotic PoultryStar® sol to broilers in drinking water. To this purpose, three Ross 308 broiler flocks, representing separate progenies of a breeder flock which was treated with the same synbiotic, were housed in separate farms, divided into treatment and control groups, and followed throughout the productive cycle. Synbiotic administration was shown to improve gut health even in absence of a challenge, with limited changes in terms of macroscopic intestinal lesions and more overt differences related to histopathological scores and villi length. Synbiotic-fed chickens performed consistently better in terms of body weight gain, feed conversion ratio and survivability. Lastly, the evaluation of the caecal microbiome through next-generation sequencing highlighted the effects of synbiotic supplementation on the composition of the bacterial population, the implications of which will, however, require further studies to be better comprehended.</jats:p

Effects of a Multi-Genus Synbiotic (PoultryStar® sol) on Gut Health and Performance of Broiler Breeders

In recent years, a rising interest has been directed towards the use of nutraceuticals in the zootechnical sector, including probiotics, prebiotics, and synbiotics, as a way to support production efficiency and cope with the increasing limitations to the use of antibiotics. In poultry, however, most studies on these products have been conducted on broilers, while less information is available on their benefits to other productive categories. The present field study aimed to assess the effects of a multi-species synbiotic product (PoultryStar® sol) on the gut health and productive performance of broiler breeders. A total of 24761 day-old Ross 308 parent stock chicks were acquired from a single hatchery and placed on the same farm. Female chicks were divided into three groups and raised in different houses (A, B, and C), in which males were introduced at the age of mating and followed until 40 weeks of age. The synbiotic was provided by drinking water to the flocks in houses A and B, while house C was kept as control. Following the manufacturer’s guidelines, the product was administered intermittently once every two weeks, except in the first and the twenty-first week when it was supplied for three consecutive days. Data on performance parameters, egg quality traits, bacterial enteritis scoring, intestinal morphometry, and histopathology were recorded, and the caecal content was collected at 15, 25, and 40 weeks of age to investigate the intestinal microbiota using high-throughput next-generation sequencing. Synbiotic-treated hens showed significantly higher survivability during production compared to the control group. No clear differences were observed between treated and control chickens in terms of egg production and quality, and the effect of the synbiotic on weight gain also appeared limited. From 25 weeks onwards, synbiotic-treated chickens scored better in terms of macroscopical lesions and had longer intestinal villi. Significant differences in crypt length and histopathological lesions were also found at multiple sampling points. A treatment effect on caecal bacterial composition was detected with a differential abundance of Gastranaerophilales, Lachnospiraceae, Helicobacter, Ruminococcaceae, and Clostridia, among others. Taken together, obtained results support the beneficial effects of the intermittent administration of the synbiotic product PoultryStar® sol on the gut health of broiler breeders. Keywords: Broiler breeder, Gastrointestinal health, Histopathology, Microbiota, Synbiotic</jats:p

Additional file 1: of Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis

Denaturing gradient gel electrophoresis (DGGE) fingerprint of DNA samples of duodenal content applying community PCR with universal bacterial primers targeting the variable V3 region of the 16S ribosomal RNA (18 animals per group (3 pens/group, 6 animals/pen)). Percentage of similarity between DGGE profiles was analyzed using the Dice similarity coefficient, derived from presence or absence of bands. On the basis of a distance matrix, which was generated from the similarity values, dendrograms were constructed using the unweighted pair group method with arithmetic means (UPGMA) as clustering-method. The microbial richness (R) was assessed as the number of OTUs within a profile. Treatment is not reflected by DGGE fingerprint. Independently of treatment three clades are distinguishable concerning the diversity of OTUs: (A)one clade with reduced number of bands built by 2 FBs-samples and 3 control-samples, a second clade (B)with average diversity between 10 and 15 OTUs across the medium GC-range and a third clade (C)consisting of 18 to 31 OTUs again in the medium but also high GC-range

Additional file 2: of Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis

Denaturing gradient gel electrophoresis (DGGE) fingerprint of DNA samples of jejunal content applying community PCR with universal bacterial primers targeting the variable V3 region of the 16S ribosomal RNA (18 animals per group (3 pens/group, 6 animals/pen)). Percentage of similarity between DGGE profiles was analyzed using the Dice similarity coefficient, derived from presence or absence of bands. On the basis of a distance matrix, which was generated from the similarity values, dendrograms were constructed using the unweighted pair group method with arithmetic means (UPGMA) as clustering-method. The microbial richness (R) was assessed as the number of OTUs within a profile. Treatment is not reflected by DGGE fingerprint. (A)No difference in number of OTUs was demonstrated. In general, all samples OTUs were located in the medium range of GC-content. (1)The banding-pattern of bird 8, control group, is shifted to the lower GC-range what flags it unique in comparison to the others which hardly exhibit bands in this area