Assessment of genetic diversity in French bean (Phaseolus vulgaris L) based on RAPD marker

RAPD molecular markers were used to evaluate the genetic diversity in the fourteen varieties of French bean (Phaseolus vulgaris) of three eco-geographical regions of Bangladesh. Out of the 20 primers only, 6 yielded polymorphic banding patterns. In total, 40 different DNA bands were reproducibly obtained, out of which 28 (70%) were polymorphic. The polymorphisms were scored and used in band-sharing analysis to identify genetic relationships. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped all the 14 genotypes into two major groups. Pair-wise variety comparison of the varieties showed that inter-variety similarity indices for PV004 versus PV005 (98.51) was higher than all other varietals pairs. On the other hand, inter-cultivar similarity index for BARI 01 versus PV009 (59.58) cultivar was lower than all other cultivar pairs. Therefore, the inherent simplicity and efficiency of RAPD analyses will be to facilitate the construction of RAPD-based genetic linkage maps in French bean.Key words: RAPD, French bean, polymorphism

“Application Of AI And Blockchain In Healthcare Industry”

This paper explores the multifaceted applications of AI and Blockchain in the healthcare industry, with a scope encompassing their impact on patient care, data security, and operational processes. The primary objectives include providing a comprehensive overview of these technologies, highlighting their potential benefits, identifying key challenges, proposing solutions, and offering insights into the future of healthcare as AI and Blockchain evolve. The integration of AI and Blockchain holds promise for revolutionizing healthcare, offering patient-centric care, and ensuring data security while addressing regulatory, ethical, and interoperability issues that currently pose challenges to their adoptio

Etiological Profile and Treatment Outcome of Epistaxis at a Tertiary Care Hospital in Northwestern Tanzania: A Prospective Review of 104 Cases.

Epistaxis is the commonest otolaryngological emergency affecting up to 60% of the population in their lifetime, with 6% requiring medical attention. There is paucity of published data regarding the management of epistaxis in Tanzania, especially the study area. This study was conducted to describe the etiological profile and treatment outcome of epistaxis at Bugando Medical Centre, a tertiary care hospital in Northwestern Tanzania. This was a prospective descriptive study of the cases of epistaxis managed at Bugando Medical Centre from January 2008 to December 2010. Data collected were analyzed using SPSS computer software version 15. A total of 104 patients with epistaxis were studied. Males were affected twice more than the females (2.7:1). Their mean age was 32.24 ± 12.54 years (range 4 to 82 years). The modal age group was 31-40 years. The commonest cause of epistaxis was trauma (30.8%) followed by idiopathic (26.9%) and hypertension (17.3%). Anterior nasal bleeding was noted in majority of the patients (88.7%). Non surgical measures such as observation alone (40.4%) and anterior nasal packing (38.5%) were the main intervention methods in 98.1% of cases. Surgical measures mainly intranasal tumor resection was carried out in 1.9% of cases. Arterial ligation and endovascular embolization were not performed. Complication rate was 3.8%. The overall mean of hospital stay was 7.2 ± 1.6 days (range 1 to 24 days). Five patients died giving a mortality rate of 4.8%. Trauma resulting from road traffic crush (RTC) remains the most common etiological factor for epistaxis in our setting. Most cases were successfully managed with conservative (non-surgical) treatment alone and surgical intervention with its potential complications may not be necessary in most cases and should be the last resort. Reducing the incidence of trauma from RTC will reduce the incidence of emergency epistaxis in our centre

Modeling the optimal mitigation of potential impact of climate change on coastal ecosystems.

Global warming is adversely affecting the earth's climate system due to rapid emissions of greenhouse gases (GHGs). Consequently, the world's coastal ecosystems are rapidly approaching a dangerous situation. In this study, we formulate a mathematical model to assess the impact of rapid emissions of GHGs on climate change and coastal ecosystems. Furthermore, we develop a mitigation method involving two control strategies: coastal greenbelt and desulfurization. Here, greenbelt is considered in coastal areas to reduce the concentrations of GHGs by absorbing the environmental carbon dioxide (CO2), whereas desulfurization is considered in factories and industries to reduce GHG emissions by controlling the release of harmful sulfur compounds. The model and how it can control the situation are analytically verified. Numerical results of this study are confirmed by comparison with other studies that examine different scenarios. Results show that both control strategies can mitigate GHG concentrations, curtail global warming and to some extent manage climate change. The results further reveal that both control strategies are more effective than one control method. Overall, the results suggest that the concentrations of GHGs and the effects of climate change can be controlled by adopting sufficient coastal greenbelt and desulfurization techniques in various industries

Positive and negative well-being and objectively measured sedentary behaviour in older adults: evidence from three cohorts

Background: Sedentary behaviour is related to poorer health independently of time spent in moderate to vigorous physical activity. The aim of this study was to investigate whether wellbeing or symptoms of anxiety or depression predict sedentary behaviour in older adults. Method: Participants were drawn from the Lothian Birth Cohort 1936 (LBC1936) (n = 271), and the West of Scotland Twenty-07 1950s (n = 309) and 1930s (n = 118) cohorts. Sedentary outcomes, sedentary time, and number of sit-to-stand transitions, were measured with a three-dimensional accelerometer (activPAL activity monitor) worn for 7 days. In the Twenty-07 cohorts, symptoms of anxiety and depression were assessed in 2008 and sedentary outcomes were assessed ~ 8 years later in 2015 and 2016. In the LBC1936 cohort, wellbeing and symptoms of anxiety and depression were assessed concurrently with sedentary behaviour in 2015 and 2016. We tested for an association between wellbeing, anxiety or depression and the sedentary outcomes using multivariate regression analysis. Results: We observed no association between wellbeing or symptoms of anxiety and the sedentary outcomes. Symptoms of depression were positively associated with sedentary time in the LBC1936 and Twenty-07 1950s cohort, and negatively associated with number of sit-to-stand transitions in the LBC1936. Meta-analytic estimates of the association between depressive symptoms and sedentary time or number of sit-to-stand transitions, adjusted for age, sex, BMI, long-standing illness, and education, were β = 0.11 (95% CI = 0.03, 0.18) and β = − 0.11 (95% CI = − 0.19, −0.03) respectively. Conclusion: Our findings indicate that depressive symptoms are positively associated with sedentary behavior. Future studies should investigate the causal direction of this association

Study of pesticide use on bitter gourd production at Jashore district

The study was designed to assess the extent of pesticide use and profitability of bitter gourd production at farm level in selected areas of Jashore district during January-May, 2019. Average farm size was 0.79 ha and average bitter gourd cultivated area per farmer was 0.07 ha. Most of the farmer cultivated local variety, which is called Gajkorola and some farmer cultivated hybrid korola such as Gonggajoli, Tia and BARI Korola-1 variety. About ninety-nine percent farmers sprayed insecticides and fungicides in their fields to protect crops from different insect pests and diseases. Thirty-nine percent farmers used pheromone trap for crop protection. A farmer averagely used 11.19 times spray in their fields and its range was 5-18 times in a season. The average yield of bitter gourd was 16.74 t ha-1 for non-IPM farmer and 16.16 t ha-1 for IPM farmer. The average total cost of production of bitter gourd was Tk. 203984 ha-1 for non-IPM farmer and Tk. 191246 ha-1 for IPM farmer. Gross return was Tk. 389705 ha-1 and net return was Tk. 185721 ha-1 for non-IPM farmer. While, gross return of IPM farmer was Tk. 360828 ha-1 and net return was Tk. 169582 ha-1. Benefit cost ratio was 1.91 and 1.89 for non-IPM and IPM farmer, respectively that means bitter gourd production was profitable. Cent percent farmer reported that IPM technology was time-consuming method and it was not only the measures to protect pests. Int. J. Agril. Res. Innov. Tech. 10(2): 110-115, December 202

Are we failing to protect threatened mangroves in the Sundarbans world heritage ecosystem?

The Sundarbans, the largest mangrove ecosystem in the world, is under threat from historical and future human exploitation and sea level rise. Limited scientific knowledge on the spatial ecology of the mangroves in this world heritage ecosystem has been a major impediment to conservation efforts. Here, for the first time, we report on habitat suitability analyses and spatial density maps for the four most prominent mangrove species - Heritiera fomes, Excoecaria agallocha, Ceriops decandra and Xylocarpus mekongensis. Globally endangered H. fomes abundances declined as salinity increased. Responses to nutrients, elevation, and stem density varied between species. H. fomes and X. mekongensis preferred upstream habitats. E. agallocha and C. decandra preferred down-stream and mid-stream habitats. Historical harvesting had negative influences on H. fomes, C. decandra and X. mekongensis abundances. The established protected area network does not support the most suitable habitats of these threatened species. We therefore recommend a reconfiguration of the network to include these suitable habitats and ensure their immediate protection. These novel habitat insights and spatial predictions can form the basis for future forest studies and spatial conservation planning, and have implications for more effective conservation of the Sundarbans mangroves and the many other species that rely on them

Revisiting the mechanism of coagulation factor XIII activation and regulation from a structure/functional perspective

The activation and regulation of coagulation Factor XIII (FXIII) protein has been the subject of active research for the past three decades. Although discrete evidence exists on various aspects of FXIII activation and regulation a combinatorial structure/functional view in this regard is lacking. In this study, we present results of a structure/function study of the functional chain of events for FXIII. Our study shows how subtle chronological submolecular changes within calcium binding sites can bring about the detailed transformation of the zymogenic FXIII to its activated form especially in the context of FXIIIA and FXIIIB subunit interactions. We demonstrate what aspects of FXIII are important for the stabilization (first calcium binding site) of its zymogenic form and the possible modes of deactivation (thrombin mediated secondary cleavage) of the activated form. Our study for the first time provides a structural outlook of the FXIIIA 2 B 2 heterotetramer assembly, its association and dissociation. The FXIIIB subunits regulatory role in the overall process has also been elaborated upon. In summary, this study provides detailed structural insight into the mechanisms of FXIII activation and regulation that can be used as a template for the development of future highly specific therapeutic inhibitors targeting FXIII in pathological conditions like thrombosis

Impact of substrate on soilless tomato cultivation

An experiment was carried out to determine the effect of four different media based hydroponics on plant growth, yield and nutritional values at Biochemistry laboratory of Patuakhali Science and Technology University (PSTU), Bangladesh during November 2014 to April 2015. Tomato plants were grown in closed soilless system where Hoagland solution as nutrient solution and jute fiber, cotton (jhut), coconut husk as substrate. Among four types of media, the media composed with Hoagland solution and jute fiber showed good impact on growth and nutritional values than the other three media (media of Hoagland solution with coconut husk, Hoagland solution with cotton and only Hoagland solution. It was revealed that the highest plant height, yield, vitamin C, fruit protein, fat and fiber content of all were related to media combination of jute fiber and Hoagland solution. Among all the verities, the highest plant height (106 cm), yield (5.3 kg plant-1), fruit Vitamin C content (64.54 mg 100 g-1), fruit protein (17.67 %), fat (5.2%) and fiber (7.9%) content was recorded from Patharkuchi tomato variety.Int. J. Agril. Res. Innov. & Tech. 6 (2): 82-86, December, 201