Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution.

The ability to directly visualize nanoscopic cellular structures and their spatial relationship in all three dimensions will greatly enhance our understanding of molecular processes in cells. Here we demonstrated multicolor three-dimensional (3D) stochastic optical reconstruction microscopy (STORM) as a tool to quantitatively probe cellular structures and their interactions. To facilitate STORM imaging, we generated photoswitchable probes in several distinct colors by covalently linking a photoswitchable cyanine reporter and an activator molecule to assist bioconjugation. We performed 3D localization in conjunction with focal plane scanning and correction for refractive index mismatch to obtain whole-cell images with a spatial resolution of 20-30 nm and 60-70 nm in the lateral and axial dimensions, respectively. Using this approach, we imaged the entire mitochondrial network in fixed monkey kidney BS-C-1 cells, and studied the spatial relationship between mitochondria and microtubules. The 3D STORM images resolved mitochondrial morphologies as well as mitochondria-microtubule contacts that were obscured in conventional fluorescence images

Simultaneous Formation of CH₃NH₃PbI₃ and electron transport layers using antisolvent method for efficient perovskite solar cells

A new antisolvent method was developed to prepare CH₃NH₃PbI₃ and electron transport layers for making efficient hybrid perovskite solar cells. By directly using [6,6]-phenyl-C61-butyric acid methyl ester in chlorobenzene solution as antisolvent, CH₃NH₃PbI₃ and electron transport layers were simultaneously formed in the films. This method not only simplifies the fabrication process of devices, but also produces uniform perovskite films and improves the interfacial structures between CH₃NH₃PbI₃ and electron transport layers. Large perovskite grains were observed in these films, with the average grain size of >1 μm. The so-formed CH₃NH₃PbI₃/electron transport layers demonstrated good optical and charge transport properties. And perovskite solar cells fabricated using these simultaneously-formed layers achieved a higher power conversion efficiency of 16.58% compared to conventional antisolvent method (14.92%). This method reduces nearly 80% usage of chlorobenzene during the fabrication, offering a more facile and environment-friendly approach to fabricate efficient perovskite solar cells than the conventional antisolvent method

Physics perspectives of heavy-ion collisions at very high energy

Heavy-ion collisions at very high colliding energies are expected to produce a quark-gluon plasma (QGP) at the highest temperature obtainable in a laboratory setting. Experimental studies of these reactions can provide an unprecedented range of information on properties of the QGP at high temperatures. We report theoretical investigations of the physics perspectives of heavy-ion collisions at a future high-energy collider. These include initial parton production, collective expansion of the dense medium, jet quenching, heavy-quark transport, dissociation and regeneration of quarkonia, photon and dilepton production. We illustrate the potential of future experimental studies of the initial particle production and formation of QGP at the highest temperature to provide constraints on properties of strongly interaction matter.Comment: 35 pages in Latex, 29 figure

Some Operations on Quaternion Numbers

In this article, we give some equality and basic theorems about quaternion numbers, and some special operations.Li Bo - Qingdao University of Science and Technology, ChinaLiang Xiquan - Qingdao University of Science and Technology, ChinaWang Pan - Qingdao University of Science and Technology, ChinaZhuang Yanping - Qingdao University of Science and Technology, ChinaGrzegorz Bancerek. The ordinal numbers. Formalized Mathematics, 1(1):91-96, 1990.Czesław Byliński. The complex numbers. Formalized Mathematics, 1(3):507-513, 1990.Czesław Byliński. Functions and their basic properties. Formalized Mathematics, 1(1):55-65, 1990.Czesław Byliński. Functions from a set to a set. Formalized Mathematics, 1(1):153-164, 1990.Czesław Byliński. Some basic properties of sets. Formalized Mathematics, 1(1):47-53, 1990.Fuguo Ge. Inner products, group, ring of quaternion numbers. Formalized Mathematics, 16(2):135-139, 2008, doi:10.2478/v10037-008-0019-x.Krzysztof Hryniewiecki. Basic properties of real numbers. Formalized Mathematics, 1(1):35-40, 1990.Xiquan Liang and Fuguo Ge. The quaternion numbers. Formalized Mathematics, 14(4):161-169, 2006, doi:10.2478/v10037-006-0020-1.Andrzej Trybulec. Enumerated sets. Formalized Mathematics, 1(1):25-34, 1990.Andrzej Trybulec and Czesław Byliński. Some properties of real numbers. Formalized Mathematics, 1(3):445-449, 1990.Zinaida Trybulec. Properties of subsets. Formalized Mathematics, 1(1):67-71, 1990.Edmund Woronowicz. Relations and their basic properties. Formalized Mathematics, 1(1):73-83, 1990.Edmund Woronowicz. Relations defined on sets. Formalized Mathematics, 1(1):181-186, 1990

Experimental Investigation on the Influence of the Oil Return Hole on the Performance of R-32 Wet Compression Cycle

R-32 has been being one of the hot candidate for refrigerant substitute because of its better thermodynamic performance. In this study, the influence of wet compression on R-32 system performance was researched by theoretical analysis and experimental test firstly. The result showed that, with the suction dryness decreasing, the discharge temperature，and the volumetric efficiency, and the system performance decreased simultaneously. And then, on the base of the wet compression experiment test, the influence of the oil return hole in the gas-liquid separator on system performance of R32 wet compression was compared. The experimental results showed that, in wet compression, the decreasing rate, that the cooling capacity and EER decreased with the discharge temperature decreasing, decreased with the size of the oil return hole increasing. The increase of the oil return hole size was benefit to improve the system performance in wet compression. But it increased the risk of over wet compression or liquid impact for compressor under frosting and defrosting condition