Toxins for Transgenic Resistance to Hemipteran Pests

The sap sucking insects (Hemiptera), which include aphids, whiteflies, plant bugs and stink bugs, have emerged as major agricultural pests. The Hemiptera cause direct damage by feeding on crops, and in some cases indirect damage by transmission of plant viruses. Current management relies almost exclusively on application of classical chemical insecticides. While the development of transgenic crops expressing toxins derived from the bacterium Bacillus thuringiensis (Bt) has provided effective plant protection against some insect pests, Bt toxins exhibit little toxicity against sap sucking insects. Indeed, the pest status of some Hemiptera on Bt-transgenic plants has increased in the absence of pesticide application. The increased pest status of numerous hemipteran species, combined with increased prevalence of resistance to chemical insecticides, provides impetus for the development of biologically based, alternative management strategies. Here, we provide an overview of approaches toward transgenic resistance to hemipteran pests

A Captured Runaway Black Hole in NGC 1277?

Recent results indicate that the compact lenticular galaxy NGC 1277 in the Perseus Cluster contains a black hole of approximately 10 billion solar masses. This far exceeds the expected mass of the central black hole in a galaxy of the modest dimensions of NGC 1277. We suggest that this giant black hole was ejected from the nearby giant galaxy NGC 1275 and subsequently captured by NGC 1277. The ejection was the result of gravitational radiation recoil when two large black holes merged following the merger of two giant ellipticals that helped to form NGC 1275. The black hole wandered in the cluster core until it was captured in a close encounter with NGC 1277. The migration of black holes in clusters may be a common occurrence.Comment: Four pages, accepted by The Astrophysical Journal Letters. Major revisions, especially Section

Autographa californica multiple nucleopolyhedrovirus ODV-E56 envelope protein is required for oral infectivity and can be substituted functionally by Rachiplusia ou multiple nucleopolyhedrovirus ODV-E56

he Autographa californica multiple nucleopolyhedrovirus (AcMNPV) odv-e56gene encodes an occlusion-derived virus (ODV)-specific envelope protein, ODV-E56. In a previous analysis, the odv-e56 gene was found to be under positive selection pressure, suggesting that it may be a determinant of virus host range. To assess the role of ODV-E56 in oral infectivity and host range, we constructed recombinant AcMNPV clones (Ac69GFP-e56lacZ and AcIEGFP-e56lacZ) in which ODV-E56 protein synthesis was eliminated by inserting a β-galactosidase (lacZ) expression cassette into the odv-e56 open reading frame. We also constructed a recombinant virus, Ac69GFP-Roe56, in which the native AcMNPV odv-e56 coding sequence was replaced with that of Rachiplusia oumultiple nucleopolyhedrovirus (RoMNPV), a closely related virus that is significantly more virulent towards some host species than AcMNPV. The odv-e56 recombinant viruses exhibited no alterations in polyhedron production and morphogenesis or in the production of infectious budded virus in cell culture. In bioassays using three lepidopteran host species, the oral infectivities of the odv-e56 mutant viruses Ac69GFP-e56lacZ and AcIEGFP-e56lacZ were profoundly impaired compared with those of wild-type and control recombinant viruses. Oral infectivity was restored fully by marker rescue of the odv-e56 mutant viruses with either the AcMNPV or the RoMNPVodv-e56 gene. In bioassays using two host species that are more susceptible to RoMNPV than to AcMNPV, Ac69GFP-Roe56 killed larvae with LC50 values similar to those of recombinant viruses expressing AcMNPV ODV-E56. This result indicated that replacement of the AcMNPV odv-e56 gene with the RoMNPV orthologue did not increase virulence against these two species

The Black Hole Mass - Galaxy Luminosity Relationship for Sloan Digital Sky Survey Quasars

We investigate the relationship between the mass of the central supermassive black hole, M_bh, and the host galaxy luminosity, L_gal, in a sample of quasars from the Sloan Digital Sky Survey (SDSS) Data Release 7 (DR7). We use composite quasar spectra binned by black hole mass and redshift to assess galaxy features that would otherwise be overwhelmed by noise in individual spectra. The black hole mass is calculated using the photoionization method, and the host galaxy luminosity is inferred from the depth of the Ca II H + K features in the composite spectra. We evaluate the evolution in the M_bh - L_gal relationship by examining the redshift dependence of Delta log M_bh, the offset in black hole mass from the local black hole - bulge relationship. There is little systematic trend in Delta log M_bh out to z = 0.8. Using the width of the [O III] emission line as a proxy for the stellar velocity dispersion, sigma_*, we find agreement of our derived host luminosities with the locally-observed Faber-Jackson relation. This supports the utility of the width of the [O III] line as a proxy for sigma_* in statistical studies.Comment: 10 pages, 5 figures; final version; major revision

Deep Sequencing of the Transcriptomes of Soybean Aphid and Associated Endosymbionts

Background The soybean aphid has significantly impacted soybean production in the U.S. Transcriptomic analyses were conducted for further insight into leads for potential novel management strategies. Methodology/Principal Findings Transcriptomic data were generated from whole aphids and from 2,000 aphid guts using an Illumina GAII sequencer. The sequence data were assembled de novo using the Velvet assembler. In addition to providing a general overview, we demonstrate (i) the use of the Multiple-k/Multiple-C method for de novo assembly of short read sequences, followed by BLAST annotation of contigs for increased transcript identification: From 400,000 contigs analyzed, 16,257 non-redundant BLAST hits were identified; (ii) analysis of species distributions of top non-redundant hits: 80% of BLAST hits (minimum e-value of 1.0-E3) were to the pea aphid or other aphid species, representing about half of the pea aphid genes; (iii) comparison of relative depth of sequence coverage to relative transcript abundance for genes with high (membrane alanyl aminopeptidase N) or low transcript abundance; (iv) analysis of theBuchnera transcriptome: Transcripts from 57.6% of the genes from Buchnera aphidicola were identified; (v) identification of Arsenophonus and Wolbachia as potential secondary endosymbionts; (vi) alignment of full length sequences from RNA-seq data for the putative salivary gland protein C002, the silencing of which has potential for aphid management, and the putative Bacillus thuringiensis Cry toxin receptors, aminopeptidase N and alkaline phosphatase. Conclusions/Significance This study provides the most comprehensive data set to date for soybean aphid gene expression: This work also illustrates the utility of short-read transcriptome sequencing and the Multiple-k/Multiple-C method followed by BLAST annotation for rapid identification of target genes for organisms for which reference genome sequences are not available, and extends the utility to include the transcriptomes of endosymbionts

Disk-Jet Connection in Agns and Microquasars: The Possibility of Thermal Flares in the Center

We discuss the possibility of thermal flares in centers of AGNs and microquasars. We present preliminary results of an ongoing study trying to assess the feasibility of a hypothesis suggesting that certain flares observed in these sources originate in the very centers of the systems and not in the relativistic jets. Using a simple toy model we reproduce optical flares with lightcurves very similar to those observed in the sources. The model suits especially well those cases where only the latter peak of a double-peaked optical flare has a radio counterpart.Comment: 6 pages, 1 figure, to appear in the proceedings of the HEPRO II conference, Buenos Aires, October 26-30 200

Microbial Control of Black Cutworm (Lepidoptera: Noctuidae) in Turfgrass Using Agrotis ipsilon Multiple Nucleopolyhedrovirus

Agrotis ipsilon multiple nucleopolyhedrovirus (family Baculoviridae, genusNucleopolyhedrovirus, AgipMNPV), a naturally occurring baculovirus, was found infecting black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), on central Kentucky golf courses. Laboratory, greenhouse, and field studies investigated the potential of AgipMNPV for managing black cutworms in turfgrass. The virus was highly active against first instars (LC50 = 73 occlusion bodies [OBs] per μl with 2-μl dose; 95% confidence intervals, 55–98). First instars that ingested a high lethal dose stopped feeding and died in 3–6 d as early second instars, whereas lethally infected fourth instars continued to feed and grow for 4–9 d until death. Sublethal doses consumed by third or fifth instars had little or no effect on subsequent developmental rate or pupal weight. Horizontal transmission of AgipMNPV in turfgrass plots was shown. Sprayed suspensions of AgipMNPV (5 × 108–6 × 109 OBs/m2) resulted in 75 to \u3e93% lethal infection of third or fourth instars in field plots of fairway-height creeping bentgrass, Agrostis stolonifera (Huds.), and on a golf course putting green collar. Virus spray residues (7 × 109 OBs/m2) allowed to weather on mowed and irrigated creeping bentgrass field plots significantly increased lethal infection of implanted larvae for at least 4 wk. This study, the first to evaluate a virus against a pest in turfgrass, suggests that AgipMNPV has potential as a preventive bioinsecticide targeting early instar black cutworms. Establishing a virus reservoir in the thatch and soil could suppress successive generations of that key pest on golf courses and sport fields

Simulated Sunlight-UV Sensitivity of Engineered Juvenile Hormone Esterase and Scorpion Toxin Recombinants of the Nuclear Polyhedrosis Virus of Autographa californica

There has been an effort over the last decade to enhance the effectiveness of wild-type baculoviruses using genetic engineering. Wild-type viruses are extremely sensitive to sunlight-ultraviolet, but, what about engineered, recombinant baculoviruses? We found that insertion of a foreign gene did not result in recombinant baculoviruses being more or less sensitive to simulated sunlight-UV than a parental wild-type baculovirus. The half-life of activity for all recombinants and the wild-type parental isolate we tested was within that previously reported for other baculoviruses