DISPONIBILIDAD DE FOSFORO EN UN ANDISOL, CON DISTINTAS FUENTES Y DOSIS DE FOSFORO, EN CONDICIONES CONTROLADAS

Phosphate is an essential element for plant nutrition, and therefore it is important to know the dynamics of sources of phosphorus (P) with different solubility in soils that naturally have low levels of available P and that fixate or adsorb the aggregated P. For that purpose four doses of P were applied (0, 300, 600 y 1200 mg.kg-1) to an Andisol soil type from the IX Region (Chile), employing fertilizers that differ in their solubility, triple superphosphate (TSP) and Rock phosphate (RF), and afterwards they were maintained in controlled temperature and humidity conditions. Available P – (Olsen) was measured 1, 7, 14, 28 and 120 days after applications. The treatments that received TSP, showed a rapid increase in available P, followed by an initial rapid decrease on the first day and later by a slow steady decrease. When RF was applied the level of available P increase slowly in time, but never reaches the levels attained with TSP. The P adsorption after one day of applying TSP, was explained by a lineal regression, Available P=0.07xP applied + 0.64 (R2=0.99; P<0.001). Nevertheless the complete period adsorption was better explained by a logarithmic function.El fósforo es un elemento esencial en la nutrición de las plantas, por lo que es importante conocer el comportamiento de fuentes de P con distinta solubilidad en suelos que naturalmente tienen bajas cantidades de P disponible y fijan o adsorben el P agregado. Con este propósito a un suelo Andisol de la IX Región de Chile, se le aplicaron 4 dosis de P (0, 300, 600 y 1200 mg.kg-1) provenientes de dos fuentes fertilizan-tes de distinta solubilidad, Superfosfato Triple (SFT) y Roca fosfórica (RF) y se incubaron en condiciones controladas de temperatura y humedad. En todos los tratamientos se midió el P – disponible (Olsen) en los días 1, 7, 14, 28 y 120. Los suelos que recibieron el SFT, presentaron un aumento rápido de P disponible, luego un descenso acelerado en los primeros días y después siguió disminuyendo lentamente. Al aplicar RF el nivel de P disponible fue aumentando en el tiempo lentamente sin alcanzar, durante todo el experimento, la disponibilidad de P lograda con SFT. La adsorción del P al primer día después de la aplicación de SFT, se explicó con una función lineal, P disponible = 0,07x P aplicado + 0,64 (R2=0,99; P<0,001). Sin embargo, el comportamiento de todo el período se describió con una curva logarítmica

Nutritional relationships in bitter pit-affected fruit and the feasibility of Vis-NIR models to determine calcium concentration in Fuji apples.

Fuji is among the most cultivated apples worldwide but affected by the disorder bitter pit (BP). Calcium deficiency plays an important role on fruit susceptibility to BP. The objectives of this study were to determine nutritional relationships in BP-affected fruit and to verify if Vis-NIR models can predict Ca concentration in Fuji apples. Fruit was harvested during 2018 season from two different orchards with historical high BP incidence. Seven hundred and fifty apples were stored at 0 ?C for 150 days plus 10 days at 20 ?C for BP assessments. After storage, 20 fruit with BP symptoms (BP+) and 20 healthy fruit (BP?) were assessed individually for mineral concentration. Vis-NIR evaluation involved a spectra range from 285 to 1200 nm to predict Ca concentration from ?Fuji? powder enriched Ca solutions. In each orchard, healthy apples had significantly higher Ca concentration than apples with BP. The K/Ca and Mg/Ca ratios were significantly lower in healthy fruit compared with BP? affected fruit. The relationship B/Ca proved to be significant in BP fruit. Although Ca interaction with organic substances and/or cellular structures could influence NIR spectra in fresh fruit, our results showed that Vis-NIR models could not be used to direct prediction of fruit Ca concentration

Unveiling the assessment process behind an integrated flood risk management plan

In the context of the European Floods Directive, flood risk assessment is a critical component for the definition of an integrated management plan that operates within a multidimensional landscape shaped by intricate interactions. This study explores this complex interplay using a comprehensive framework, aimed at enlightening the non-linear pathways that flood risk assessments can traverse. It adopts the Gioia Methodology within the Grounded Theory approaches, enabling a nuanced exploration of flood risk assessment dynamics. Utilizing data from an Italian case study in the Po River District, this study unveils the flood risk assessment process framework by identifying 13 first-order codes, 6 s-order themes and 3 aggregate dimensions. It introduces a qualitative self-assessment tool to facilitate integration across dimensions and enhance Directive alignment, offering valuable insights for future flood risk assessment implementations

Music benefits on postoperative distress and pain in pediatric day care surgery

Postoperative effect of music listening has not been established in pediatric age. Response on postoperative distress and pain in pediatric day care surgery has been evaluated. Forty-two children were enrolled. Patients were randomly assigned to the music-group (music intervention during awakening period) or the non-music group (standard postoperative care). Slow and fast classical music and pauses were recorded and played via ambient speakers. Heart rate, blood pressure, oxygen saturation, glucose and cortisol levels, faces pain scale and Face, Legs, Activity, Cry, Consolability (FLACC) Pain Scale were considered as indicators of response to stress and pain experience. Music during awakening induced lower increase of systolic and diastolic blood pressure levels. The non-music group showed progressive increasing values of glycemia; in music-group the curve of glycemia presented a plateau pattern (P<0.001). Positive impact on reactions to pain was noted using the FLACC scale. Music improves cardiovascular parameters, stress-induced hyperglycemia. Amelioration on pain perception is more evident in older children. Positive effects seems to be achieved by the alternation of fast, slow rhythms and pauses even in pediatric age

Allosteric Modulation of the HIV-1 gp120-gp41 Association Site by Adjacent gp120 Variable Region 1 (V1) N-Glycans Linked to Neutralization Sensitivity

The HIV-1 gp120-gp41 complex, which mediates viral fusion and cellular entry, undergoes rapid evolution within its external glycan shield to enable escape from neutralizing antibody (NAb). Understanding how conserved protein determinants retain functionality in the context of such evolution is important for their evaluation and exploitation as potential drug and/ or vaccine targets. In this study, we examined how the conserved gp120-gp41 association site, formed by the N- and Cterminal segments of gp120 and the disulfide-bonded region (DSR) of gp41, adapts to glycan changes that are linked to neutralization sensitivity. To this end, a DSR mutant virus (K601D) with defective gp120-association was sequentially passaged in peripheral blood mononuclear cells to select suppressor mutations. We reasoned that the locations of suppressors point to structural elements that are functionally linked to the gp120-gp41 association site. In culture 1, gp120 association and viral replication was restored by loss of the conserved glycan at Asn136 in V1 (T138N mutation) inconjunction with the L494I substitution in C5 within the association site. In culture 2, replication was restored with deletion of the N139INN sequence, which ablates the overlapping Asn141-Asn142-Ser-Ser potential N-linked glycosylation sequons inV1, in conjunction with D601N in the DSR. The 136 and 142 glycan mutations appeared to exert their suppressive effects by altering the dependence of gp120-gp41 interactions on the DSR residues, Leu593, Trp596 and Lys601. The 136 and/or 142glycan mutations increased the sensitivity of HIV-1 pseudovirions to the glycan-dependent NAbs 2G12 and PG16, and also pooled IgG obtained from HIV-1-infected individuals. Thus adjacent V1 glycans allosterically modulate the distal gp120-gp41 association site. We propose that this represents a mechanism for functional adaptation of the gp120-gp41 association site to an evolving glycan shield in a setting of NAb selection

Ammonium excess leads to Ca restrictions, morphological changes, and nutritional imbalances in tomato plants, which can be monitored by the N/Ca ratio.

Both nitrogen and calcium fertilization management are vital for crops, where an imbalance of these elements can cause both physiological and yield problems. It has been proposed that nitrogen absorption, particularly ammonium, is in part dependent on calcium supply. Moreover, the balance between these two nutrients could be a key indicator of plant growth in some species. Tomato, one of the most cultivated crops worldwide, can also be widely affected by nutritional imbalance. Using large amounts of N fertilizers could lead to an imbalance with other nutrients and, thus, detrimental effects in terms of plant development and yield. Here we show that ammonium excess has a negative impact on plant development and results in calcium deficiency. Moreover, a deficit in calcium nutrition not only affects calcium concentration but also leads to a restriction in N uptake and reduced N concentration in the plant. These effects were evident at the seedling stage and also during flowering/fruit set. Using PCA analysis, we integrated both phenotypic and nutritional imbalances in seedlings and grown plants. Interestingly, the Ca/N ratio appears to be a key indicator to monitor appropriate N and calcium nutrition and more importantly the balance between both. Maintaining this balance could be an essential element for tomato crop production

Reference gene validation for quantitative RT-PCR during biotic and abiotic stresses in Vitis vinifera

Grapevine is one of the most cultivated fruit crop worldwide with Vitis vinifera being the species with the highest economical importance. Being highly susceptible to fungal pathogens and increasingly affected by environmental factors, it has become an important agricultural research area, where gene expression analysis plays a fundamental role. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is currently amongst the most powerful techniques to perform gene expression studies. Nevertheless, accurate gene expression quantification strongly relies on appropriate reference gene selection for sample normalization. Concerning V. vinifera, limited information still exists as for which genes are the most suitable to be used as reference under particular experimental conditions. In this work, seven candidate genes were investigated for their stability in grapevine samples referring to four distinct stresses (Erysiphe necator, wounding and UV-C irradiation in leaves and Phaeomoniella chlamydospora colonization in wood). The expression stability was evaluated using geNorm, NormFinder and BestKeeper. In all cases, full agreement was not observed for the three methods. To provide comprehensive rankings integrating the three different programs, for each treatment, a consensus ranking was created using a non-weighted unsupervised rank aggregation method. According to the last, the three most suitable reference genes to be used in grapevine leaves, regardless of the stress, are UBC, VAG and PEP. For the P. chlamydospora treatment, EF1, CYP and UBC were the best scoring genes. Acquaintance of the most suitable reference genes to be used in grapevine samples can contribute for accurate gene expression quantification in forthcoming studiesinfo:eu-repo/semantics/publishedVersio

Search for a resonance decaying to a W boson and a photon in proton-proton collisions at s \sqrt{s} = 13 TeV using leptonic W boson decays

A search for a new charged particle X with mass between 0.3 and 2.0 TeV decaying to a W boson and a photon is presented, using proton-proton collision data at a center-of-mass energy of 13 TeV, collected by the CMS experiment and corresponding to an integrated luminosity of 138 fb−1. Particle X has electric charge ±1 and is assumed to have spin 0. The search is performed using the electron and muon decays of the W boson. No significant excess above the predicted background is observed. The upper limit at 95% confidence level on the product of the production cross section of the X and its branching fraction to a W boson and a photon is found to be 94 (137) fb for a 0.3 TeV resonance and 0.75 (0.81) fb for a 2.0 TeV resonance, for an X width-to-mass ratio of 0.01% (5%). This search presents the most stringent constraints to date on the existence of such resonances across the probed mass range. A statistical combination with an earlier study based on the hadronic decay mode of the W boson is also performed, and the upper limit at 95% confidence level for a 2.0 TeV resonance is reduced to 0.50 (0.63) fb for an X width-to-mass ratio of 0.01% (5%)

Search for the Z Boson Decay to ττμμ in Proton-Proton Collisions at √s = 13 TeV

The first search for the boson decay to ⁢⁢⁢ at the CERN LHC is presented, based on data collected by the CMS experiment at the LHC in proton-proton collisions at a center-of-mass energy of 13 TeV and corresponding to an integrated luminosity of 138  fb−1. The data are compatible with the predicted background. For the first time, an upper limit at the 95% confidence level of 6.9 times the standard model expectation is placed on the ratio of the →⁢⁢⁢ to →4⁢ branching fractions. Limits are also placed on the six flavor-conserving four-lepton effective-field-theory operators involving two muons and two tau leptons, for the first time testing all such operators