血清脂質と非アルコール性脂肪肝疾患関連染色体領域における遺伝的探索

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Present Condition of Pet Allergy - Based on a Comparison between the Conditions of Raising Pets by Healthy People Living in a Rural Area and by Asthma Patients Living in an Urban Area-

The large majority of people in the rural area healthy student group raised dogs, mainly as watchdogs, often outdoors. On the other hand, a great part of the people in the urban area asthma patient group kept cats mainly as pets, 100% indoors. The asthma patient group had significantly higher positive rates of intracutaneous reactions to feline and canine epidermis antigens. Therefore, the more frequently people contacted pets raised indoors, the more easily they were sensitized. Pets that were not well cared for had a lot of feline dandruff antigen and are called pets in an antigen-contaminated state. There is danger of even healthy people being sensitized without being aware of it. To prevent against pet allergies, it is important to use every measure available to avoid each antigen.departmental bulletin pape

Methionine regulates copper/hydrogen peroxide oxidation products of Aβ

Metal-catalysed oxidation (MCO) may play a causative role in the pathogenesis of Alzheimer\u27s disease (AD). Amyloid peptide (A), the major biomarker of AD, in the presence of copper ions reduces Cu2+ to Cu+ and catalyses the formation of H2O2 that subsequently induces radicals through Fenton chemistry. A is also subject to attack by free radicals, where the presence of Cu2+ in conjunction with H2O2 catalyses oxygenation, primarily at the methionine sulfur atom. This work investigates MCO of A, to gain further insight into the role of oxidative stress in AD. By combining a fluorescence assay with gel electrophoresis to monitor MCO reactions of A (1-28) in the presence and absence of methionine it was determined that methionine can both protect some residues against MCO and promote the oxidation of Tyr(10) specifically. Electrospray ionization mass spectrometric analysis of methionine MCO products indicated the formation of methionine sulfoxide, methionine sulfone and related hydroxylated products. Similar products could be formed from the oxidation of Met(35) of A and may relate to changes in properties of the peptide following MCO. <br /

Çavuşoğlu, “Identification and active exploration of deformable object boundary constraints through robotic manipulation,” The International journal of robotics research

Abstrac

Identification of deleterious rare variants in MTTP, PNPLA3, and TM6SF2 in Japanese males and association studies with NAFLD

Abstract Background Non-alcoholic fatty liver disease (NAFLD) is a disorder characterized by excessive fat deposits in hepatocytes without excessive alcohol intake. NAFLD is influenced by genetic factors, and the heritability has been estimated at 0.35 to 0.6 by twin studies. We explored rare variants in known NAFLD-associated genes to investigate whether these rare variants are involved in the susceptibility to NAFLD. Methods The target genes for re-sequencing were PNPLA3, TM6SF2, and MTTP. All exons of these three genes were amplified from a discovery panel of 950 Japanese males, and the identified rare variants were further tested for genetic association in 3014 individuals from the Japanese general population and for in vitro functional evaluation. Results Target re-sequencing analysis using next-generation sequencing identified 29 rare variants in 65 Japanese males (6.84%), 12 of which were newly identified base substitutions. A splicing mutation in TM6SF2 that resulted in deletion of 31 amino acids was identified in an NAFLD case. Among eight genotyped rare single-nucleotide polymorphisms (SNPs; minor allele frequency < 0.02), rs143392071 (Tyr220Cys, PNPLA3) significantly increased (odds ratio = 3.52, P = 0.008) and rs756998920 (Val42Ile, MTTP) significantly decreased (odds ratio = 0.03, P = 0.019) the NAFLD risk. Functional assays showed that these two SNPs disrupted protein functions and supported the genetic association. Conclusion Collectively, 1.79% of individuals in our studied population were estimated carriers of rare variants that are potentially associated with NAFLD