Effects of single mutations on the stability of horseradish peroxidase to hydrogen peroxide

Horseradish peroxidase (HRP) is a commonly used enzyme in many biotechnological fields. Improvement of HRP stability would further increase its potential application range. In the present study, 13 single- and three double-mutants of solvent exposed, proximal lysine and glutamic acid residues were analysed for enhanced H2O2 stability. Additionally, five single- and one pentuple-consensus mutants were investigated. Most mutants displayed little or no alteration in H2O2 stability; however, three (K232N, K241F and T110V) exhibited significantly increased H2O2 tolerances of 25- (T110V), 18- (K232N), and 12-fold (K241F). This improved stability may be due to an altered enzyme-H2O2 catalysis pathway or to removal of potentially oxidisable residues

AUTOMATION TO PREVENT QUERY PARAMETER EXPOSURE ON HYPERTEXT TRANSFER PROTOCOL GET REQUESTS

Techniques are provided to allow an existing web application to seamlessly use custom HyperText Transfer Protocol (HTTP) headers under the hood. This resolves an entire category of web security problems without a major redesign at the feature level of code

Trace gases and CO2 isotope records from cabo de rama, India

Concentrations of carbon dioxide (CO2), methane (CH4), carbon monoxide (CO), nitrous oxide (N2O) and hydrogen (H2), and the stable carbon (δ 13C-CO2) and oxygen (δ 18O-CO2) isotopic composition of CO2 have been measured in air samples collected from Cabo de Rama (CRI), India, for the period 1993-2002. The observations show clear signatures of Northern and Southern Hemispheric (NH and SH) air masses, mixed with their regional fluxes and chemical loss mechanisms, resulting in complex seasonal variation of these gases. The CRI measurements are compared with remote marine sites at Seychelles and Mauna Loa. Simulations of two major anthropogenic greenhouse gases (CO2 and CH4) concentrations using a chemistry-transport model for the CRI site suggest that globally optimized fluxes can produce results comparable to the observations. We discuss that CRI observations have provided critical guidance in optimizing the fluxes to constrain the regional source/sinks balance

Building trust in deep learning-based immune response predictors with interpretable explanations

The ability to predict whether a peptide will get presented on Major Histocompatibility Complex (MHC) class I molecules has profound implications in designing vaccines. Numerous deep learning-based predictors for peptide presentation on MHC class I molecules exist with high levels of accuracy. However, these MHC class I predictors are treated as black-box functions, providing little insight into their decision making. To build turst in these predictors, it is crucial to understand the rationale behind their decisions with human-interpretable explanations. We present MHCXAI, eXplainable AI (XAI) techniques to help interpret the outputs from MHC class I predictors in terms of input peptide features. In our experiments, we explain the outputs of four state-of-the-art MHC class I predictors over a large dataset of peptides and MHC alleles. Additionally, we evaluate the reliability of the explanations by comparing against ground truth and checking their robustness. MHCXAI seeks to increase understanding of deep learning-based predictors in the immune response domain and build trust with validated explanations

Best Surveillance System

The GPS module fitted in the bus records the accurate longitudinal and latitudinal location of the bus. As the location of the bus changes it will keep on sending the new latitude and longitude value to the base center. This way each bus will update itself with the main server. Each bus will be identified by its own unique identification number. Now when an person who wants to travel by a particular bus number sends a message to the base center. The base center will reply him giving the current location of the bus by which the person wants to travel.This way the user of the system will know the real time location of the bus and in what time it can reach to the expected destination DOI: 10.17762/ijritcc2321-8169.150519

Technologies and Techniques for Detection of Nitrate in potable water: A Review

In agricultural and industrial production nitrate is widely used. Nitrate is appearing in water, environment, biology and food. Nitrate is a toxic inorganic contaminant, that’s why it is perilous to the health living organisms and humans. In recent years, a variety of techniques has been developed for monitoring of nitrate. This paper aggregated as a general survey of the techniques proposed for nitrate monitoring and important monitoring parameters (such as detection limit, working pH, detection range and materials) were classified. This paper is composed of the sort of signal got from techniques, including optical and electrical signals.Electrochemical techniques get an electric signal from dissolved nitrate, with impedimetric, potentiometric and voltammetric techniques are included. Raman Spectrometry, fluorescence and absorption techniques receive an optical signal. In recent years, Biosensors are proposed for monitoring the nitrate in water. The limitations and disadvantages/advantages of the techniques are discussed in this paper. At last, techniques employed to perform nitrate, monitoring is summarized, and their future scope and development are discussed

ARM Based Easy water Distribution and Data Recording System

ARM based easy water distribution system is used to distribute water from centre system. This system includes various sensors like humidity sensor, water level sensor and moisture sensor. Humidity sensor is used to measure humidity of environment at field and there is another sensor to measure water capacity in the tank. These sensors are attached with the raspberry pi board. These sensors sense the data and automatically send it to central controller. System has capability to control water pump to release water in farm but when after some threshold level reached in water tank. Sensors at field continuously send the data to central system. This central system monitor the data related to water and humidity and send the water whenever it required on the field .In this System no user interaction required directly. These all sensed data are recorded at central system and user can monitor this data whenever he wants in future. This System helpful to automatic management of water distribution and monitoring of it and it also saves the time and water. This is an embedded system. DOI: 10.17762/ijritcc2321-8169.15054

Survey On Ensuring Distributed Accountability for Data Sharing in the Cloud

Cloud computing is the use of computing of sources that are delivered as a service over a network for example on internet. It enables highly scalable services to be easily utilized over the Internet on an as needed basis. Important characteristic of the cloud services is that users’ data are usually processed remotely in unknown machines that users do not operate. It can become a substantial barrier to the wide taking on cloud services. To address this problem highly decentralized responsibility framework to keep track of the actual usage of the user’s data in the cloud. In this work has automated logging and distributed auditing mechanism. The Cloud Information Accountability framework proposed in this work conducts distributed auditing of relevant access performed by any entity, carried out at any point of time at any cloud service provider. It conations two major elements: logger and log harmonizer. This methodology will also take concern of the JAR file by converting the JAR into obfuscated code which will adds an additional layer of security to the infrastructure. Rather than this here in this work, increase the security of user’s data by provable data control for integrity verificatio

Result Paper: (TMS320 F 28027)Microcontroller Based PWM Signal Generation for Speed Control of DC Motor

Inany modern factory all types of electromechanical devices are used such as AC motors, DC motors, power amplifiers. Therefore there is serious need of intelligent devices capable of driving and controlling a wide range of electrical and electromechanical devices. For controlling the speed of DC motor series architecture of variable resistors is not good because it drops excess of energy and flux and armature control methods cannot provide speed control in the desirable range. Therefore DC motor is controlled by Voltage control method in which PWM signal is used to control electromechanical devices and this PWM signal is generated from analog, digital IC and microcontroller. PWM signal obtained from analog or digital IC contains harmonics, therefore mostly PWM signals are generated from (TMS 320 F 28027) Microcontroller. It reduces the hardware complexity and it consumes less power. PWM signal generated using modifying CCP register of microcontroller. DC motor is interfaced with TMS microcontroller through H-bridge driver L-293D. H-bridge control the motion of DC motor. PWM signal is generated at four duty cycles for increasing or decreasing the speed of motor

A rare case of thoraco omphalopagus twins

Conjoined twins, popularly referred as Siamese twins, are twins joined in utero, affecting less than 1% of the monozygotic pairs. The frequency of conjoined twins has prevalence of 1.5 in 1 lakh births and thoracopagus is the most common. Early diagnosis is important as many patients opt for termination because of the grave prognosis. Though the prognosis of conjoined twin is generally low, there is limited evidence regarding the optimum method of termination of the pregnancy particularly in advanced gestational age. We hereby, report a rare case of successful medical termination of thoraco-omphalopagus twins at 23 weeks and 2 days of gestation. A 27-years old, 2nd gravid woman with previous vaginal delivery diagnosed with conjoined thoraco-omphalopagus twin pregnancy at 23 weeks and 2 days of gestation after the detailed ultrasonographic evaluation. Proper assessment and counselling were done regarding prognosis. Different options of termination were discussed and patient opted for medical termination of pregnancy with possibility of reverting to hysterotomy in case difficulty is encountered. After a 2 day of cervical preparation, a successful vaginal delivery was conducted. Even though this is an experience from a single case, medical termination can be safely preformed in carefully selected cases of conjoined twins beyond 20 weeks of gestation. The adequate cervical preparation, pain control and careful monitoring of the procedure are critical for optimal outcome