Construction and Test of a Cryocatcher Prototype for SIS100

The main accelerator SIS100 of the FAIR-complex will provide heavy ion beams of highest intensities. Beam loss due to ionization is the most demanding loss mechanism at operation with high intensity, intermediate charge state heavy ions. A special synchrotron design has been devel- oped for SIS100, aiming for hundred percent control of ion- ization beam loss by means of a dedicated cryogenic ion catcher system. To suppress dynamic vacuum effects, the cryo catcher system shall provide a significantly reduced effective desorption yield. The construction and test of a prototype cryocatcher is a task of the EU-FP-7 workpack- age COLMAT. A prototype test setup, including cryostat has been constructed, manufactured and tested under real- istic conditions with beams from the heavy ion synchrotron SIS18. The design and results are presented

Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype

Abstract Background Recent studies have indicated the presence of multipotent mesenchymal stromal cells (MSCs) in human lung diseases. Excess airway smooth muscle, myofibroblasts and activated fibroblasts have each been noted in asthma, suggesting that mesenchymal progenitor cells play a role in asthma pathogenesis. We therefore sought to determine whether MSCs are present in the lungs of ovalbumin (OVA)-sensitized and challenged mice, a model of allergic airways disease. Methods Balb/c mice were sensitized and challenged with PBS or OVA over a 25 day period. Flow cytometry as well as colony forming and differentiation potential were used to analyze the emergence of MSCs along with gene expression studies using immunochemical analyses, quantitative polymerase chain reaction (qPCR), and gene expression beadchips. Results A CD45-negative subset of cells expressed Stro-1, Sca-1, CD73 and CD105. Selection for these markers and negative selection against CD45 yielded a population of cells capable of adipogenic, osteogenic and chondrogenic differentiation. Lungs from OVA-treated mice demonstrated a greater average colony forming unit-fibroblast (CFU-F) than control mice. Sorted cells differed from unsorted lung adherent cells, exhibiting a pattern of gene expression nearly identical to bone marrow-derived sorted cells. Finally, cells isolated from the bronchoalveolar lavage of a human asthma patient showed identical patterns of cell surface markers and differentiation potential. Conclusions In summary, allergen sensitization and challenge is accompanied by an increase of MSCs resident in the lungs that may regulate inflammatory and fibrotic responses.http://deepblue.lib.umich.edu/bitstream/2027.42/78265/1/1465-9921-11-127.xmlhttp://deepblue.lib.umich.edu/bitstream/2027.42/78265/2/1465-9921-11-127.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/78265/3/1465-9921-11-127-S1.DOCPeer Reviewe

EuCARD Newsletter Issue 3

European Coordination for Accelerator Research and Development (EuCARD) Newsletter Issue 3: October - December 2009 * A word on behalf of the Steering Committee * Cryocatcher in the GSI * Strategy and the Spallation Source * Accelerators for hadron therapy * For EuCARD members: publication

Law and Grace in the Work of St. Ilarion, Metropolitan of Kyiv (1051-1054 AD)

Protocole d'entente entre l'Université Laval et l'Université de SherbrookeLa Grande Commission donnée aux Apôtres par le Seigneur Jésus Christ (Mt.28:19- 20) représente la base de la mission chrétienne non seulement à l’ère biblique ou patristique mais pendant toute l’histoire de l’Église du Christ. Chacun des Apôtres et missionnaires est chargé d’expliquer le besoin du Baptême. Pourquoi devenir chrétien? St Paul l’Apôtre explique la nécessité de la Foi et du Baptême en jouant sur le contraste et la comparaison entre la Foi et la Loi, le Baptême et la circoncision, le Christ et Moïse, la Nouvelle Alliance et l’Ancienne. Dans le Prologue de l’Évangile St Jean l’Évangéliste parle de la Nouvelle Alliance établie sur Jésus Christ Dieu-Homme. “Car la Loi fut donnée par Moïse; la grâce et la vérité sont venues par Jésus Christ” (Jn 1:17). St Ilarion de Kyiv en Rous’-Kyivienne du XIe siècle a proclamé le Sermon pascal basé sur ces mots de la péricope de l’Évangile pascale qui sont lus liturgiquement dans l’Église orthodoxe. St Ilarion commence son Sermon sur la Loi et la Grâce en continuant la parole de St Jean: “Sur la loi, donnée par Moïse, sur la Grâce et la Vérité qui le furent par Jésus Christ”. Donc la littérature de la Rous’-Kyivienne commence avec un sermon néo-patristique signifiant le remplacement de la Loi par la Grâce du Christ et aussi manifestant la joie que l’Église de la Nouvelle Alliance a apportée au peuple de la Rous’-Kyivienne. “La vraie Foi se répandit sur toute la terre. Elle parvint aussi jusqu’à notre peuple de la Rous’. Le lac de la Loi s’assécha, tandis que la source de l’Évangile, après s’être gorgée d’eau et avoir recouvert toute la terre, se déversa jusque chez nous.” (Lines 240-243).The Great Commission given by the Lord Jesus Christ to the Apostles (Mt. 28:19 – 20) is the basis of the Christian mission not only in the Biblical and Patristic eras but throughout the growth of the Christian Church. Every Apostle and Missionary was charged to explain the need for repentance and Baptism. Why be a Christian? St. Paul the Apostle explains the necessity of Faith and Baptism by comparing and contrasting Faith and Law; Baptism and Circumcision; Christ and Moses; the New Covenant and The Old. St. John the Evangelist in his Prologue to his Gospel speaks of the New Covenant based on the Person of Jesus Christ. For the Law was given through Moses; grace and truth came through Jesus Christ. (John 1:17). St. Ilarion of Kyiv in eleventh century Kyivan-Rus’ proclaimed a Paschal Sermon built upon these words of St. John which are the last words of the traditional pericope read at Pascha in the Orthodox Church and began his sermon: Concerning the Law given by Moses, and concerning Grace and Truth which came through Jesus Christ. Thus the literature of Kyivan-Rus’ begins with a neo – patristic sermon expressing the supersedence of the Law of Moses by the Grace of Christ and also the great joy that the New Covenant Church has reached the people of Kyivan-Rus’. Our Good God did not forget any corner of the world, nor us; He desired and saved us and brought us to true understanding. (Lines 258 – 259)

Mesenchymal Stromal Cells from Neonatal Tracheal Aspirates Demonstrate a Pattern of Lung-Specific Gene Expression

We have previously isolated mesenchymal stromal cells (MSCs) from the tracheal aspirates of premature neonates with respiratory distress. Although isolation of MSCs correlates with the development of bronchopulmonary dysplasia, the physiologic role of these cells remains unclear. To address this, we further characterized the cells, focusing on the issues of gene expression, origin, and cytokine expression. Microarray comparison of early passage neonatal lung MSC gene expression to cord blood MSCs and human fetal and neonatal lung fibroblast lines demonstrated that the neonatal lung MSCs differentially expressed 971 gene probes compared with cord blood MSCs, including the transcription factors Tbx2, Tbx3, Wnt5a, FoxF1, and Gli2, each of which has been associated with lung development. Compared with lung fibroblasts, 710 gene probe transcripts were differentially expressed by the lung MSCs, including IL-6 and IL-8/CXCL8. Differential chemokine expression was confirmed by protein analysis. Further, neonatal lung MSCs exhibited a pattern of Hox gene expression distinct from cord blood MSCs but similar to human fetal lung fibroblasts, consistent with a lung origin. On the other hand, limiting dilution analysis showed that fetal lung fibroblasts form colonies at a significantly lower rate than MSCs, and fibroblasts failed to undergo differentiation along adipogenic, osteogenic, and chondrogenic lineages. In conclusion, MSCs isolated from neonatal tracheal aspirates demonstrate a pattern of lung-specific gene expression, are distinct from lung fibroblasts, and secrete pro-inflammatory cytokines.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90487/1/scd-2E2010-2E0494.pd