Extending Practical (In Use) Shelf Life of Oncology Drug Vials Using Spikes

The global increase of use of oncology drugs combined with the higher costs of thesedrugs raise the question of how to reduce these costs. One way to reduce the costs is toreduce spillage by extending the beyond-use date by preserving remainders in the vialof (expensive) oncology drugs instead of wasting them. Therefore, we investigated ifspikes, instead of the expensive closed-system transfer devices, can be used to extendthe beyond-use date of drugs both at room temperature and in the refrigerator duringseven days after reconstitution and partial fluid withdrawal of a vial. Six hundred vialscontaining concentrated tryptic soy broth were reconstituted with 10-mL of sodiumchloride 0.9%, after which approximately 3 ml were removed from the vial anddiscarded using a regular spike for 300 vials and a MicroSpike for the other 300 vials.Subsequently, the vials were stored either at refrigerator temperature or at roomtemperature for seven days. After seven days, all six hundred vials were transported andincubated at a temperature of 30°C to 35°C for fourteen days. None of the six hundredvials used showed contamination, either punctured with a MicroSpike or with a regularspike, after storage of seven days at room temperature or in the refrigerator and twoweeks of incubation. Conclusively, it can be stated that hospital pharmacies play animportant role in keeping the high costs of oncology drugs as low as possible. This studyshows that using a spike instead of a more expensive closed-system transfer device forpreservation of the remainder of oncology drugs will further reduce spillage ofexpensive drugs resulting in lower healthcare costs

Dynamic Nuclear Polarization of Silicon Carbide Micro- and Nanoparticles

[Image: see text] Two dominant crystalline phases of silicon carbide (SiC): α-SiC and β-SiC, differing in size and chemical composition, were investigated regarding their potential for dynamic nuclear polarization (DNP). (29)Si nuclei in α-SiC micro- and nanoparticles with sizes ranging from 650 nm to 2.2 μm and minimal oxidation were successfully hyperpolarized without the use of free radicals, while β-SiC samples did not display appreciable degrees of polarization under the same polarization conditions. Long T(1) relaxation times in α-SiC of up to 1600 s (∼27 min) were recorded for the (29)Si nuclei after 1 h of polarization at a temperature of 4 K. Interestingly, these promising α-SiC particles allowed for direct hyperpolarization of both (29)Si and (13)C nuclei, resulting in comparably strong signal amplifications. Moreover, the T(1) relaxation time of (13)C nuclei in 750 nm-sized α-SiC particles was over 33 min, which far exceeds T(1) times of conventional (13)C DNP probes with values in the order of 1–2 min. The present work demonstrates the feasibility of DNP on SiC micro- and nanoparticles and highlights their potential as hyperpolarized magnetic resonance imaging agents

Imaging Hyperpolarized Pyruvate and Lactate after Blood-Brain Barrier Disruption with Focused Ultrasound

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Anatomic reconstruction or temporary screw stabilization of chronic symptomatic anterolateral proximal tibiofibular joint instability

The paper describes the new concept Tensairity which can be used to significantly improve the load bearing capacity of inflatable wings. The basic principle of Tensairity is to use an inflatable structure to stabilize conventional compression and tension elements. So far, Tensairity has been mainly used in civil engineering application like roof structures and bridges. In this work, considerations to apply Tensairity to wing structures are given and the construction of two wing-like Tensairity kite prototypes is described. Test results on the Tensairity structure used in these kites are presented and compared to purely air inflated structures. Finally, the advantages of Tensairity wings are discussed and some application areas of these wings are suggested.Aerospace Design, Integration and OperationsAerospace Engineerin

Applications of isothermal titration calorimetry - the research and technical developments from 2011 to 2015

Isothermal titration calorimetry is a widely used biophysical technique for studying the formation or dissociation of molecular complexes. Over the last 5years, much work has been published on the interpretation of isothermal titration calorimetry (ITC) data for single binding and multiple binding sites. As over 80% of ITC papers are on macromolecules of biological origin, this interpretation is challenging. Some researchers have attempted to link the thermodynamics constants to events at the molecular level. This review highlights work carried out using binding sites characterized using x-ray crystallography techniques that allow speculation about individual bond formation and the displacement of individual water molecules during ligand binding and link these events to the thermodynamic constants for binding. The review also considers research conducted with synthetic binding partners where specific binding events like anion-π and π-π interactions were studied. The revival of assays that enable both thermodynamic and kinetic information to be collected from ITC data is highlighted. Lastly, published criticism of ITC research from a physical chemistry perspective is appraised and practical advice provided for researchers unfamiliar with thermodynamics and its interpretation

A distinct role for B1b lymphocytes in T cell-independent immunity

Pathogenesis of infectious disease is not only determined by the virulence of the microbe but also by the immune status of the host. Vaccination is the most effective means to control infectious diseases. A hallmark of the adaptive immune system is the generation of B cell memory, which provides a long-lasting protective antibody response that is central to the concept of vaccination. Recent studies revealed a distinct function for B1b lymphocytes, a minor subset of mature B cells that closely resembles that of memory B cells in a number of aspects. In contrast to the development of conventional B cell memory, which requires the formation of germinal centers and T cells, the development of B1b cell-mediated long-lasting antibody responses occurs independent of T cell help. T cell-independent (TI) antigens are important virulence factors expressed by a number of bacterial pathogens, including those associated with biological threats. TI antigens cannot be processed and presented to T cells and therefore are known to possess restricted T cell-dependent (TD) immunogenicity. Nevertheless, specific recognition of TI antigens by B1b cells and the highly protective antibody responses mounted by them clearly indicate a crucial role for this subset of B cells. Understanding the mechanisms of long-term immunity conferred by B1b cells may lead to improved vaccine efficacy for a variety of TI antigens

Successful Introduction of Peanut in Sensitized Infants With Reported Reactions at Home

Background and objective: Previous studies have shown efficacy of early introduction of peanut to prevent peanut allergy. It is currently unknown which diagnostic pathway is optimal after parental-reported reactions to peanut at home after early introduction. Methods: The PeanutNL cohort study included high-risk infants who were referred for early introduction of peanut. A subgroup of 186 infants with reactions to peanut at home underwent peanut skin prick tests and a supervised open oral food challenge (OFC) at a median age of 8 months. After a negative OFC, peanut was introduced at home. Results: Sensitization to peanut was detected in 69% of 186 infants, of whom 80% had &gt;4 mm wheals in skin prick tests. An OFC with a cumulative dose of 4.4 g of peanut protein was performed in 163 infants with Sampson severity score grade I-III reactions at home; 120 challenges were negative. Peanut was subsequently introduced at home in infants with a negative challenge outcome. After 6 months, 96% were still eating peanut and 81% ate single portions of 3.0 g of peanut protein. One patient was considered to be peanut allergic after reintroduction of peanut at home. Conclusions: These data show that 65% of infants with reported reactions to peanut at home have negative OFCs. In those children, peanut could be introduced safely, and 96% were able to consume peanut regularly without reactions. Challenging infants younger than 12 months prevents the misdiagnosis of peanut allergy and enables safe continued exposure to peanut and the induction of long-term tolerance.</p

The prevalence of peanut-triggered food protein-induced enterocolitis syndrome in a prospective cohort of infants introducing peanut in the first year of life

Background: Since the early introduction of peanut to prevent IgE-mediated peanut allergy, other case series have suggested an increased incidence of peanut-triggered Food Protein Induced Enterocolitis Syndrome (FPIES). Data on the prevalence of peanut-induced FPIES in prospective cohorts are lacking. Methods: The PeanutNL cohort is a prospective cohort that included infants at risk of peanut allergy (n = 706) as well as infants with reactions to peanut at home after early introduction (n = 186). They all introduced peanut before the age of 12 months. Oral food challenges were performed to introduce peanut or to evaluate reactions to peanut at home. Results: Of the 706 infants that were included for first introduction of peanut, 2 had reactions with a phenotype compatible with FPIES (0.3%). Of the 186 infants with reactions to peanut at home, 6 were diagnosed with FPIES (3.2%). Seven out of 8 cases had ingestions of peanut without reactions at home or during clinical introduction before FPIES became apparent. During a 3-year follow-up, six infants (75%) were shown to be tolerant to peanut before the age of 3 years. Conclusion: The prevalence of challenge-proven peanut-induced FPIES in a Dutch cohort of atopic infants that introduced peanut between the ages of 4 and 11 months is 0.3%. The majority of cases were tolerant to peanut before the age of 3 years. When introducing peanut in the first year of life, physicians should be aware of FPIES reactions, but it should not be a reason to avoid early introduction of peanut.</p