Teaching percutaneous renal biopsy using unfixed human cadavers

Background: Percutaneous renal biopsy (PRB) is an important diagnostic procedure. Despite advances in its safety profile there remains a small but significant risk of bleeding complications. Traditionally, operators train to perform PRB through tutor instruction and directly supervised PRB attempts on real patients. We describe an approach to teaching operators to perform PRB using cadaveric simulation. Methods: We devised a full day course hosted in the Clinical Anatomy Skills Centre, with places for nine candidates. Course faculty consisted of two Consultant Nephrologists, two Nephrology trainees experienced in PRB, and one Radiologist. Classroom instruction included discussion of PRB indications, risk minimisation, and management of complications. Two faculty members acted as models for the demonstration of kidney localisation using real-time ultrasound scanning. PRB was demonstrated using a cadaveric model, and candidates then practised PRB using each cadaver model. Results: Written candidate feedback was universally positive. Faculty considered the cadaveric model a realistic representation of live patients, while the use of multiple cadavers introduced anatomical variation. Conclusions: Our model facilitates safe simulation of a high risk procedure. This might reduce serious harm associated with PRB and improve patient safety, benefiting trainee operators and patients alike

Risk factors for bleeding complications after nephrologist-performed native renal biopsy

Background: Bleeding is a recognized complication of native percutaneous renal biopsy. This study aimed to describe the incidence of major bleeding after biopsy in a single centre over a 15-year period and examine factors associated with major bleeding. Methods: We identified consecutive adult patients undergoing ultrasound-guided native renal biopsy in the Glasgow Renal and Transplant Unit from 2000 to 2014. From the electronic patient record, we collected data pertaining to biopsy indication, pre- and post-biopsy laboratory measurements, prescribed medication and diagnosis. Aspirin was routinely continued. We defined major bleeding post-biopsy as the need for blood transfusion, surgical or radiological intervention or death. Binary logistic regression analysis was used to assess factors associated with increased risk of major bleeding. Results: There were 2563 patients who underwent native renal biopsy (1499 elective, 1064 emergency). The average age of patients was 57 (SD 17) years and 57.4% were male. Overall, the rate of major bleeding was 2.2%. In all, 46 patients required transfusion (1.8%), 9 patients underwent embolization (0.4%), no patient required nephrectomy and 1 patient died as a result of a significant late retroperitoneal bleed. Major bleeding was more common in those undergoing emergency compared with elective renal biopsy (3.4 versus 1.1%; P < 0.001). Aspirin was being taken at the time of biopsy in 327 of 1509 patients, with no significant increase in the risk of major bleeding (P = 0.93). Body mass index (BMI) data were available for 546 patients, with no increased risk of major bleeding in 207 patients classified as obese (BMI >30). Conclusions: The risk of major bleeding following native renal biopsy in the modern era is low. Complications are more common when biopsy is conducted as an emergency, which has implications for obtaining informed consent. Our data support the strategy of not stopping aspirin before renal biopsy

Multiple socioeconomic deprivation and impact on survival in patients with primary glomerulonephritis

Background: The impact of multiple socio-economic deprivation on patient outcomes in primary renal diseases is unknown. We aimed to assess whether risk of death or requiring renal replacement therapy (RRT) in patients with primary glomerulonephritis (GN) was higher in patients living in an area of multiple socio-economic deprivation.Methods: Patients undergoing native renal biopsy between 2000 and 2014 were identified. Baseline demographics, postcode at time of biopsy, follow-up blood pressure, proteinuria and time to death or RRT were recorded. The Scottish Index of Multiple Deprivation (SIMD) is a multidimensional model used to measure deprivation based on postcode. Using SIMD, patients were separated into tertiles of deprivation.Results: A total of 797 patients were included, 64.2% were male with mean age of 54.1 (standard deviation 17.0) years. Median follow-up was 6.3 (interquartile range 3.7–9.4) years during which 174 patients required RRT and 185 patients died. Patients in the most deprived tertile of deprivation were significantly more likely to die than those in the least deprived tertile [hazard ratio (HR) 2.2, P  <  0.001], independent of age, baseline serum creatinine and blood pressure. They were not more likely to require RRT (P  =  0.22). The increased mortality risk in the most deprived tertile was not uniform across primary renal diseases, with the association being most marked in focal segmental glomerulosclerosis (HR 7.4) and IgA nephropathy (HR 2.7) and absent in membranous nephropathy.Conclusion: We have demonstrated a significant independent 2-fold increased risk of death in patients with primary GN who live in an area of multiple socio-economic deprivation at the time of diagnosis as compared with those living in less deprived areas

The Evolution of Transport Canada’s Wildlife Management and Planning Program

As a follow-up to a paper presented at International Bird Strike Committee meeting #23 in 1996, discussing Transport Canada’s emphasis on education and awareness programs as a means to reduce bird hazards to aircraft, this paper will describe significant changes that have occurred in Canada since that time. The 1994 Government of Canada National Airports Policy led to the devolution of Canada’s major airports. Private sector airport authorities now operate these airports and Transport Canada’s focus has shifted from management by policy to one of regulatory oversight. In addition to building on the awareness program that was in place in 1994, Transport Canada is in the final stages of introducing a performance-based regulation for Wildlife Management and Planning at applicable Canadian airports. Key components of the regulatory package are: a requirement for developing a risk assessment and management plan; an obligation to report all wildlife incidents; an obligation to provide training to wildlife control staff; and an obligation to establish a reporting and communication network. The applicability of the regulation is based on types of aircraft and number of operations, airport location and historical risk, and the presence of incompatible land-use activities

Immune-mediated competition in rodent malaria is most likely caused by induced changes in innate immune clearance of merozoites

Malarial infections are often genetically diverse, leading to competitive interactions between parasites. A quantitative understanding of the competition between strains is essential to understand a wide range of issues, including the evolution of virulence and drug resistance. In this study, we use dynamical-model based Bayesian inference to investigate the cause of competitive suppression of an avirulent clone of Plasmodium chabaudi (AS) by a virulent clone (AJ) in immuno-deficient and competent mice. We test whether competitive suppression is caused by clone-specific differences in one or more of the following processes: adaptive immune clearance of merozoites and parasitised red blood cells (RBCs), background loss of merozoites and parasitised RBCs, RBC age preference, RBC infection rate, burst size, and within-RBC interference. These processes were parameterised in dynamical mathematical models and fitted to experimental data. We found that just one parameter μ, the ratio of background loss rate of merozoites to invasion rate of mature RBCs, needed to be clone-specific to predict the data. Interestingly, μ was found to be the same for both clones in single-clone infections, but different between the clones in mixed infections. The size of this difference was largest in immuno-competent mice and smallest in immuno-deficient mice. This explains why competitive suppression was alleviated in immuno-deficient mice. We found that competitive suppression acts early in infection, even before the day of peak parasitaemia. These results lead us to argue that the innate immune response clearing merozoites is the most likely, but not necessarily the only, mediator of competitive interactions between virulent and avirulent clones. Moreover, in mixed infections we predict there to be an interaction between the clones and the innate immune response which induces changes in the strength of its clearance of merozoites. What this interaction is unknown, but future refinement of the model, challenged with other datasets, may lead to its discovery

Renal biopsy: it is time for pragmatism and consensus.

To obtain truly informed consent, we must be able to advise our patients accurately about the relative risk and benefit of any treatment plan. Percutaneous renal biopsy remains the gold standard investigation in the evaluation of intrinsic renal disease. There have been significant improvements in practice over the past decades with regards to percutaneous renal biopsy. Across centres, we appear now to have reached agreement on many aspects of this procedure, such as the need for blood pressure control, avoidance of coagulopathy, use of spring-loaded needles under direct imaging guidance and a need to monitor for complications. The authors from Rush University Medical Centre provide reassurance that renal biopsy in the modern era remains a safe procedure with a low rate of significant bleeding. There remain areas of divergence in practice that may have unintended and deleterious consequences: administration of desmopressin and discontinuation of aspirin, for example, both carry a risk of thrombosis. It is our opinion that it is time to reach consensus on our interpretation of the available data and to draw up guidelines to standardize our biopsy practice internationally

Producing Flexible Nurses: How Institutional Texts Organize Nurses’ Experiences of Learning to Work on Redesigned Nursing Teams (Préparer des infirmières polyvalentes : comment des documents officiels orientent les expériences d’apprentissage des infirmières en fonction du travail au sein d’équipes reconfigurées)

The purpose of this qualitative research was to utilize an Institutional Ethnographic (IE) lens to trace how various institutional (regulatory, educational, union, governmental, or health authority) texts and resources organize baccalaureate (RN) and diploma (vocational or practical) nurses’ experiences of learning to practice on acute care teams. Functional care models have been introduced in acute care, creating RN-LPN-health care aide (HCA) teams in conjunction with expanded practice scopes for LPNs. Questions arise as to how nurses (RNs and LPNs) learn to work together on these intra-professional teams. Beginning from the standpoint of front-line workers provides an entry-point into understanding how institutional priorities organize the everyday work of people. Ten RNs and ten LPNs were interviewed in two small community acute care hospitals on Vancouver Island. More specifically, in observations and interviews we looked for ways in which textually mediated work processes (such as regulatory, governmental, health authority, and educational documents) and other conceptual resources influenced nurses’ understandings of nursing education and professional practice. This analysis focused on how RNs and LPNs learn to work on re-designed nursing teams and traced the textually mediated discourses that are organizing this learning in the context of recent changes to LPN education and nursing teams. Our findings highlight unarticulated nursing knowledge/thinking, and the textual insertion of functional, skilled and flexible worker discourses, which organize to blur practice between RN and LPNs making them [potentially] interchangeable in complex acute care contexts. This study, situated as one analysis among a larger study, shows the invisibility of nursing disciplinary and professional goals and knowledge in nurses’ talk, as RNs and LPNs re-learn and sustain nursing practice in ways that fulfill other institutional and organizational goals. This re-alignment has significant implications for educators in nursing programs, who participate in teaching within educational silos. This research has shown that the absence of clarity in functional roles (perpetuating role confusion and ambiguity) is purposeful, with the goal of creating flexible workers. Résumé Cette étude qualitative selon une perspective d’ethnographie institutionnelle, visait à examiner comment divers documents institutionnels (réglementaires, académiques, syndicaux, gouvernementaux ou d’autorité régionale) orientent les expériences d’apprentissage des infirmières formées au baccalauréat (IB, en anglais RN) et des infirmières diplômées de formation professionnelle ou pratique (ID, en anglais LPN), en fonction de la pratique au sein d’équipes de soins aigus . L’intégration de modèles fonctionnels dans les soins aigus a entraîné la création d’équipes d’IBs-IDs- aides-soignantes et élargi la portée de la pratique des IDs. De cette situation surgissent des questions, à savoir comment les infirmières (IBs et IDs) apprennent à collaborer dans ces équipes intraprofessionnelles. Aborder la question du point de vue des travailleuses de première ligne offre une ouverture vers la compréhension de la manière dont les priorités institutionnelles organisent le travail quotidien de ces personnes. Nous nous sommes entretenues avec dix IB et dix ID de deux petits hôpitaux communautaires de soins aigus, sur l’île de Vancouver. Plus spécifiquement, à travers les observations et les entrevues, nous avons cherché les moyens par lesquels les processus de travail inscrits dans des textes institutionnels (documentation réglementaire, gouvernementale, d’autorité régionale et académique), ainsi que d’autres ressources conceptuelles, ont influencé la compréhension des infirmières quant à la formation et à la pratique professionnelle en sciences infirmières. Cette analyse était centrée sur la manière dont les IB et les ID apprennent à travailler au sein d’équipes infirmières reconfigurées et ciblait les énoncés de textes qui orientent cet apprentissage, dans le contexte des récents changements dans la formation des ID et au sein des équipes dont elles font partie. Nos résultats soulignent un savoir/ une pensée infirmière inexprimée et l’intégration textuelle d’un discours de travail fonctionnel, compétent et polyvalent qui contribuent à brouiller les pratiques entre les IB et les ID, les rendant [potentiellement] interchangeables en contexte de soins aigus complexes. Cette étude, qui ne représente qu’une analyse d’une étude plus large, démontre l’invisibilité de la discipline infirmière et des objectifs et savoirs professionnels dans le discours des infirmières, alors que les IB et les ID réapprennent et maintiennent une pratique infirmière qui répond à d’autres objectifs institutionnels et organisationnels. Cet ajustement a d’importantes répercussions pour les enseignantes des programmes de soins et sciences infirmières, qui évoluent de manière parallèle dans des établissements d’enseignement. Cette recherche démontre que l’absence de clarté dans les rôles fonctionnels (qui maintient la confusion de rôle et l’ambiguïté) est délibérée et vise à former des travailleuses polyvalentes