Context dependent substitution biases vary within the human genome

Background: Models of sequence evolution typically assume that different nucleotide positions evolve independently. This assumption is widely appreciated to be an over-simplification. The best known violations involve biases due to adjacent nucleotides. There have also been suggestions that biases exist at larger scales, however this possibility has not been systematically explored. Results: To address this we have developed a method which identifies over- and under-represented substitution patterns and assesses their overall impact on the evolution of genome composition. Our method is designed to account for biases at smaller pattern sizes, removing their effects. We used this method to investigate context bias in the human lineage after the divergence from chimpanzee. We examined bias effects in substitution patterns between 2 and 5 bp long and found significant effects at all sizes. This included some individual three and four base pair patterns with relatively large biases. We also found that bias effects vary across the genome, differing between transposons and non-transposons, between different classes of transposons, and also near and far from genes. Conclusions: We found that nucleotides beyond the immediately adjacent one are responsible for substantial context effects, and that these biases vary across the genome

Recommendations for Future Efforts in RANS Modeling and Simulation

The roadmap laid out in the CFD Vision 2030 document suggests that a decision to move away from RANS research needs to be made in the current timeframe (around 2020). This paper outlines industry requirements for improved predictions of turbulent flows and the cost-barrier that is often associated with reliance on scale resolving methods. Capabilities of RANS model accuracy for simple and complex flow flow fields are assessed, and modeling practices that degrade predictive accuracy are identified. Suggested research topics are identified that have the potential to improve the applicability and accuracy of RANS models. We conclude that it is important that some part of a balanced turbulence modeling research portfolio should include RANS efforts

Spiral-Induced Star Formation in the Outer Disks of Galaxies

The outer regions of galactic disks have received increased attention since ultraviolet observations with GALEX demonstrated that nearly 30% of galaxies have UV emission beyond their optical extents, indicating star formation activity. These galaxies have been termed extended UV (XUV) disks. Here, we address whether these observations contradict the gas surface density threshold for star formation inferred from Halpha radial profiles of galaxies. We run smoothed particle hydrodynamics simulations of isolated disk galaxies with fiducial star formation prescriptions and show that over-densities owing to the presence of spiral structure can induce star formation in extended gas disks. For direct comparison with observations, we use the 3-D radiative transfer code Sunrise to create simulated FUV and K_s band images. We find that galaxies classified as Type I XUV disks are a natural consequence of spiral patterns, but we are unable to reproduce Type II XUV disks. We also compare our results to studies of the Kennicutt-Schmidt relation in outer disks.Comment: Published in Ap

The Perception of Latino Mothers’ Experience with the Healthcare System in East Tennessee

As the Hispanic population continues to grow in the United States, especially in the South, it is critical for healthcare workers to provide culturally competent care as required by certain laws. The Latina experience is of significant importance due to the role Latina mothers play in their families and communities. It is necessary to understand the perspective of this population and the experience of the Latina mother in regards to healthcare; specifically, how have language barriers hindered care, what perceptions of bias or discrimination have been encountered, and how do these factors influence their healthcare decisions and outcomes. Research has been conducted that identifies language and bias as barriers to access; however, further research is necessary to identify how these barriers influence a Latina mother’s perception of the healthcare system with an emphasis placed on East Tennessee. Therefore, it is critical for more studies to be conducted in order to identify what public health officials can change in order to provide equal access to this significant demographic

Public Perceptions of Delays in the Release of Police Body-Worn Camera Footage

Delays in the release of police body-worn camera (BWC) video footage have amplified public concerns about police misconduct. Law enforcement transparency is questioned when video from BWCs is not shared in a timely manner with the community. The purpose of this qualitative case study was to explore community and victims’ family’s beliefs related to delays in the release of police BWC footage. Rogers’s diffusion innovation theory and Mettler and Sorelle’s policy feedback theory were used for the study’s conceptual framework. The research questions focused on understanding the perceptions of community relationships with law enforcement around transparency, communication, and information sharing. A qualitative phenomenology study design was used to examine 13 participants’ lived experiences and what factors influence community and victim family members’ perceptions of law enforcement when there is delay in the release of BWC video. Computer-assisted qualitative data analysis software was used, along with manual coding, to code and analyze themes that emerged from the participants’ lived experiences. The results showed that both the community and the victim’s family members are requesting changes to community policing initiatives for better community engagement and for building positive relationships, trust, transparency, police legitimacy, and communication. This study presents law enforcement and society with insight on how to improve public perceptions and understanding of appropriate use of BWC videos

Public Perceptions of Delays in the Release of Police Body-Worn Camera Footage

Delays in the release of police body-worn camera (BWC) video footage have amplified public concerns about police misconduct. People question law enforcement transparency when video from BWCs is not shared with the community in a timely manner. The qualitative case study explores the life experiences of the community and the victims’ family related to delays in the release of police BWC footage. Mettler and Sorelle’s policy feedback theory was used for the study’s theoretical framework. The research questions focus on understanding the lived experiences and perceptions of community relationships with law enforcement around transparency, communication, and information sharing. A qualitative study was used to examine 13 participants to determine which factors influence perceptions of law enforcement by the community and the victim’s family when there is delay in the release of BWC video. In addition, I focused on families who have been directly impacted and members of the community who have been indirectly impacted when police BWC video was delayed. Results show that both the community and the victim’s family members are requesting changes to community policing initiatives for better community engagement and for building positive relationships, trust, transparency, police legitimacy, and communication. This study presents law enforcement and society with insight on how to improve public perceptions

Citizens Relationships with Police Hampered by Trust, Transparency, and Underdeveloped Policies for Body Worn Cameras

This study used a critical communication conceptual framework merging the diffusion of innovation and policy feedback theory to demonstrate how policies can be formed and implemented through engaging the community. At present, there is no uniformed policies for Body Worn Cameras footage release. This study revealed a gap in the research on how families and communities are impacted when BWC video is delayed. Results showed that both the community and victim’s family members are requesting changes to community policing initiatives. Findings revealed having the community involved when polices are developed or reviewed is vital to establishing relationships with law enforcement. Recommended Citation Bush, C. L. (2020, October 1-2). Citizens relationships with police hampered by trust, transparency, and underdeveloped policies for body worn cameras [Poster presentation]. Walden University Research Conference 2020 (online). https://scholarworks.waldenu.edu/researchconference/2020/posters/53

The preventative role of exogenous melatonin administration to patients with advanced cancer who are at risk of delirium: study protocol for a randomized controlled trial

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Background Delirium is a very common and distressing neuropsychiatric syndrome in palliative care. Increasing age, the presence of dementia and advanced cancer are well-known predisposing risk factors for delirium development. Sleep-wake cycle disturbance is frequently seen during delirium and melatonin has a pivotal role in the regulation of circadian rhythms. Current evidence across various settings suggests a potential preventative role for melatonin in patients at risk of delirium, but no studies are currently reported in patients with advanced cancer. The aim of this article is to describe the design of a feasibility study that is being conducted to inform a larger randomized, placebo-controlled, double-blind trial (RCT) to evaluate the role of exogenously administered melatonin in preventing delirium in patients with advanced cancer. Methods/Design Adult patients with a cancer diagnosis who are admitted to the palliative care unit will be randomized into a treatment or placebo group. The pharmacological intervention consists of a single daily dose of immediate-release melatonin (3 mg) at 21:00 ± 1 h, from day 1 to day 28 of admission. The primary objective of this initial study is to assess the feasibility of conducting the proposed RCT by testing recruitment and retention rates, appropriateness of study outcome measures, acceptability of study procedures and effectiveness of the blinding process. The primary outcome measure of the proposed larger RCT is time to first inpatient incident episode of delirium. We also plan to collect data on incident rates of delirium and patient-days of delirium, adjusting for length of admission. Discussion The outcomes of this feasibility study will provide information on recruitment and retention rates, protocol violation frequency, effectiveness of the blinding process, acceptability of the study procedures, and safety of the proposed intervention. This will inform the design of a fully powered randomized controlled trial to evaluate the preventative role of melatonin administration in patients with advanced cancer. Trial registration Registered with ClinicalTrials.gov: NCT02200172 Registered on 21 July 2014. Health Canada protocol number: BRI-MELAT-2013 (Final approved protocol version (Version 3): 18 June 2014) (Notice of Amended Authorization (NOA) received 14 November 2014). Electronic supplementary material The online version of this article (doi:10.1186/s13063-016-1525-8) contains supplementary material, which is available to authorized users

An Arabidopsis flavonoid transporter is required for anther dehiscence and pollen development

FLOWER FLAVONOID TRANSPORTER (FFT) encodes a multidrug and toxin efflux family transporter in Arabidopsis thaliana. FFT (AtDTX35) is highly transcribed in floral tissues, the transcript being localized to epidermal guard cells, including those of the anthers, stigma, siliques and nectaries. Mutant analysis demonstrates that the absence of FFT transcript affects flavonoid levels in the plant and that the altered flavonoid metabolism has wide-ranging consequences. Root growth, seed development and germination, and pollen development, release and viability are all affected. Spectrometry of mutant versus wild-type flowers shows altered levels of a glycosylated flavonol whereas anthocyanin seems unlikely to be the substrate as previously speculated. Thus, as well as adding FFT to the incompletely described flavonoid transport network, it is found that correct reproductive development in Arabidopsis is perturbed when this particular transporter is missing