Exploring the gravitationally lensed system HE 1104-1805: Near-IR Spectroscopy

(Abridged) A new technique for the spatial deconvolution of spectra is applied to near-IR (0.95 - 2.50 micron) NTT/SOFI spectra of the lensed, radio-quiet quasar HE 1104-1805. The continuum of the lensing galaxy is revealed between 1.5 and 2.5 micron. It is used in combination with previous optical and IR photometry to infer a plausible redshift in the range 0.8 < z < 1.2. Modeling of the system shows that the lens is probably composed of the red galaxy seen between the quasar images and a more extended component associated with a galaxy cluster with fairly low velocity dispersion (~ 575 km/s). The spectra of the two lensed images of the source show no trace of reddening at the redshift of the lens nor at the redshift of the source. Additionally, the difference between the spectrum of the brightest component a nd that of a scaled version of the faintest component is a featureless continuum. Broad and narrow emission lines, including the FeII features, are perfectly subtracted. The very good quality of our spectrum makes it possible to fit precisely the optical Fe II feature, taking into account the underlying continuum over a wide wavelength range. HE 1104-1805 can be classified as a weak Fe II emitter. Finally, the slope of the continuum in the brightest image is steeper than the continuum in the faintest image and supports the finding by Wisotzki et al. (1993) that the brightest image is microlensed. This is particularly interesting in view of the new source reconstruction methods from multiwavelength photometric monitoring.Comment: to be published in A&A, 8 pages, 9 postscript figure

Genetic Abnormalities in a Calf with Congenital Increased Muscular Tonus

A 2‐week‐old female calf was referred to the Clinic for Ruminants at the Vetsuisse Faculty, University of Berne, Switzerland, with difficulty standing and muscle tremors since birth. Prior treatment by the private veterinarian with selenium, calcium, magnesium, and vitamins had not led to any improvement. The general status at arrival to the clinic was slightly reduced, though the calf was alert and attentive, and tachycardia (152/min) and tachypnea (80/min) were noticeable. Examination of the skin revealed an infected lesion on the fetlock of the left forelimb and several superficial lesions. Gastrointestinal, respiratory, and urinary tracts were without important abnormalities. The musculoskeletal system was normally developed, no atrophy was noticed, but the calf remained in a recumbent position unless lifted up and helped to stand. When standing, it showed tremor, ataxia, and could only move backward with hypermetria in the hind limbs and tip‐toe‐standing of the front limbs (Fig 1). Consciousness was normal but the calf was unable to orientate itself in its surroundings. Cranial nerve examination showed no deficits. Muscle tone was generally increased in the limbs. No painful reaction was noticed upon palpation of the limbs. The spinal reflexes were generally reduced. Sensibility was normal in the neck and shoulder area, but reduced in the limbs. The head and neck could be moved in all directions and the ears were symmetrical and loose. The clinical signs were localized in the peripheral nervous or musculoskeletal system

Evidence for Three Subpopulations of Globular Clusters in the Early-Type Post-Starburst Shell Galaxy AM 0139-655

We present deep HST ACS images of the post-starburt shell galaxy AM 0139-655. We find evidence for the presence of three distinct globular cluster subpopulations associated with this galaxy: a centrally concentrated young population (~ 0.4 Gyr), an intermediate age population (~ 1 Gyr) and an old, metal-poor population similar to that seen around normal galaxies. The g-I color distribution of the clusters is bimodal with peaks at 0.85 and 1.35. The redder peak at g-I=1.35 is consistent with the predicted color for an old metal-poor population. The clusters associated with the peak at g-I=0.85 are centrally concentrated and interpreted as a younger and more metal-rich population. We suggest that these clusters have an age of ~ 0.4 Gyr and solar metallicity based on a comparison with population synthesis models. The luminosity function of these "blue" clusters is well represented by a power law. Interestingly, the brightest shell associated with the galaxy harbors some of the youngest clusters observed. This seems to indicate that the same merger event was responsible for the formation of both the shells and the young clusters. The red part of the color distribution contains several very bright clusters, which are not expected for an old, metal-poor population. Furthermore, the luminosity function of the "red" GCs cannot be fit well by either a single gaussian or a single power law. A composite (gaussian + power law) fit to the LF of the red clusters yields both a low rms and very plausible properties for an old population plus an intermediate-age population of GCs. Hence, we suggest that the red clusters in AM 0139-655 consist of two distinct GC subpopulations, one being an old, metal-poor population as seen in normal galaxies and one having formed during a recent dissipative galaxy merger.Comment: 35 pages, 12 figures, accepted for publication in A

Enhanced heparan sulfate proteoglycan-mediated uptake of cell-penetrating peptide-modified liposomes

Protein transduction domains (PTDs) are used to enhance cellular uptake of drugs, proteins, polynucleotides or liposomes. In this study, functionalized Antennapedia (Antp, aa 43--58) and HIV Tat (aa 47-57) peptides were coupled to small unilamellar liposomes via thiol-maleimide linkage. Modified liposomes showed higher uptake into a panel of cell lines including tumor and dendritic cells than unmodified control liposomes. Liposome uptake was time and concentration dependent as analyzed by flow cytometry and live-cell microscopy. At least 100 PTD molecules per small unilamellar liposome (100 ± 30 nm) were necessary for efficient translocation into cells. Cellular uptake of PTD-modified liposomes was 15- to 25-fold increased compared to unmodified liposomes and was inhibited by preincubation of liposomes with heparin. Glycosaminoglycan-deficient CHO cells showed dramatically reduced cell association of PTD-modified liposomes, confirming the important role of heparan sulfate proteoglycans in PTD-mediated uptake. Antp-liposomes used as carriers of the cytotoxic drug N4-octadecyl-1-β-D-arabinofuranosylcytosine-(5′- 5′)-3′-C-ethinylcytidine showed a reduction of the IC50 by 70% on B16F1 melanoma cells compared with unmodified liposomes. PTD-functionalized liposomes, particularly Antp-liposomes, represent an interesting novel carrier system for enhanced cell-specific delivery of a large variety of liposome-entrapped molecule

A G1-like globular cluster in NGC 1023

The structure of a very bright (MV = -10.9) globular cluster in NGC 1023 is analyzed on two sets of images taken with the Hubble Space Telescope. From careful modeling of King profile fits to the cluster image, a core radius of 0.55+/-0.1 pc, effective radius 3.7+/-0.3 pc and a central V-band surface brightness of 12.9+/-0.5 mag / square arcsec are derived. This makes the cluster much more compact than Omega Cen, but very similar to the brightest globular cluster in M31, G1 = Mayall II. The cluster in NGC 1023 appears to be very highly flattened with an ellipticity of about 0.37, even higher than for Omega Cen and G1, and similar to the most flattened clusters in the Large Magellanic Cloud.Comment: 14 pages, 3 figures, 1 table. Accepted for AJ, Oct 200

RANKL Signaling Sustains Primary Tumor Growth in Genetically Engineered Mouse Models of Lung Adenocarcinoma.

NSCLC is the leading cause of cancer mortality. Recent retrospective clinical analyses suggest that blocking the receptor activator of NF-κB (RANK) signaling pathway inhibits the growth of NSCLC and might represent a new treatment strategy. Receptor activator of NF-κB gene (RANK) and receptor activator of NF-κB ligand gene (RANKL) expression in human lung adenocarcinoma was interrogated from publicly available gene expression data sets. Several genetically engineered mouse models were used to evaluate treatment efficacy of RANK-Fc to block RANKL, with primary tumor growth measured longitudinally using microcomputed tomography. A combination of RANKL blockade with cisplatin was tested to mirror an ongoing clinical trial. In human lung adenocarcinoma data sets, RANKL expression was associated with decreased survival and KRAS mutation, with the highest levels in tumors with co-occurring KRAS and liver kinase B1 gene (LKB1) mutations. In Kras &lt;sup&gt;LSL-G12D/WT&lt;/sup&gt; , Kras &lt;sup&gt;LSL-G12D/WT&lt;/sup&gt; ; Lkb1 &lt;sup&gt;Flox/Flox&lt;/sup&gt; and Kras &lt;sup&gt;LSL-G12D/WT&lt;/sup&gt; ; p53 &lt;sup&gt;Flox/Flox&lt;/sup&gt; mouse models of lung adenocarcinoma, we monitored an impaired progression of tumors upon RANKL blockade. Despite elevated expression of RANKL and RANK in immune cells, treatment response was not associated with major changes in the tumor immune microenvironment. Combined RANK-Fc with cisplatin revealed increased efficacy compared with that of single agents in p53- but not in Lkb1-deficient tumors. RANKL blocking agents impair the growth of primary lung tumors in several mouse models of lung adenocarcinoma and suggest that patients with KRAS-mutant lung tumors will benefit from such treatments

Correlations of Globular Cluster Properties: Their Interpretations and Uses

Correlations among the independently measured physical properties of globular clusters (GCs) can provide powerful tests for theoretical models and new insights into their dynamics, formation, and evolution. We review briefly some of the previous work, and present preliminary results from a comparative study of GC correlations in the Local Group galaxies. The results so far indicate that these diverse GC systems follow the same fundamental correlations, suggesting a commonality of formative and evolutionary processes which produce them.Comment: An invited review, to appear in "New Horizons in Globular Cluster Astronomy", eds. G. Piotto, G. Meylan, S.G. Djorgovski, and M. Riello, ASPCS, in press (2003). Latex file, 8 pages, 5 eps figures, style files include

A Population of Massive Globular Clusters in NGC 5128

We present velocity dispersion measurements of 14 globular clusters in NGC 5128 (Centarus A) obtained with the MIKE echelle spectrograph on the 6.5m Magellan Clay telescope. These clusters are among the most luminous globular clusters in NGC 5128 and have velocity dispersions comparable to the most massive clusters known in the Local Group, ranging from 10 - 30 km/s. We describe in detail our cross-correlation measurements, as well as simulations to quantify the uncertainties. These 14 globular clusters are the brightest NGC 5128 globular clusters with surface photometry and structural parameters measured from the Hubble Space Telescope. We have used these measurements to derive masses and mass-to-light ratios for all of these clusters and establish that the fundamental plane relations for globular clusters extend to an order of magnitude higher mass than in the Local Group. The mean mass-to-light ratio for the NGC 5128 clusters is ~3+/-1, higher than measurements for all but the most massive Local Group clusters. These massive clusters begin to bridge the mass gap between the most massive star clusters and the lowest-mass galaxies. We find that the properties of NGC 5128 globular clusters overlap quite well with the central properties of nucleated dwarf galaxies and ultracompact dwarf galaxies. As six of these clusters also show evidence for extratidal light, we hypothesize that at least some of these massive clusters are the nuclei of tidally stripped dwarfs.Comment: ApJ Accepted, 15 pages, 9 figures, uses emulateapj.st

Young and intermediate-age massive star clusters

An overview of our current understanding of the formation and evolution of star clusters is given, with main emphasis on high-mass clusters. Clusters form deeply embedded within dense clouds of molecular gas. Left-over gas is cleared within a few million years and, depending on the efficiency of star formation, the clusters may disperse almost immediately or remain gravitationally bound. Current evidence suggests that a few percent of star formation occurs in clusters that remain bound, although it is not yet clear if this fraction is truly universal. Internal two-body relaxation and external shocks will lead to further, gradual dissolution on timescales of up to a few hundred million years for low-mass open clusters in the Milky Way, while the most massive clusters (> 10^5 Msun) have lifetimes comparable to or exceeding the age of the Universe. The low-mass end of the initial cluster mass function is well approximated by a power-law distribution, dN/dM ~ M^{-2}, but there is mounting evidence that quiescent spiral discs form relatively few clusters with masses M > 2 x 10^5 Msun. In starburst galaxies and old globular cluster systems, this limit appears to be higher, at least several x 10^6 Msun. The difference is likely related to the higher gas densities and pressures in starburst galaxies, which allow denser, more massive giant molecular clouds to form. Low-mass clusters may thus trace star formation quite universally, while the more long-lived, massive clusters appear to form preferentially in the context of violent star formation.Comment: 21 pages, 3 figures. To appear as invited review article in a special issue of the Phil. Trans. Royal Soc. A: Ch. 9 "Star clusters as tracers of galactic star-formation histories" (ed. R. de Grijs). Fully peer reviewed. PDFLaTeX, requires rspublic.cls style fil