Understanding the role of chromatin remodeling in the regulation of circadian transcription in Drosophila.

Circadian clocks enable organisms to anticipate daily changes in the environment and coordinate temporal rhythms in physiology and behavior with the 24-h day-night cycle. The robust cycling of circadian gene expression is critical for proper timekeeping, and is regulated by transcription factor binding, RNA polymerase II (RNAPII) recruitment and elongation, and post-transcriptional mechanisms. Recently, it has become clear that dynamic alterations in chromatin landscape at the level of histone posttranslational modification and nucleosome density facilitate rhythms in transcription factor recruitment and RNAPII activity, and are essential for progression through activating and repressive phases of circadian transcription. Here, we discuss the characterization of the BRAHMA (BRM) chromatin-remodeling protein in Drosophila in the context of circadian clock regulation. By dissecting its catalytic vs. non-catalytic activities, we propose a model in which the non-catalytic activity of BRM functions to recruit repressive factors to limit the transcriptional output of CLOCK (CLK) during the active phase of circadian transcription, while the primary function of the ATP-dependent catalytic activity is to tune and prevent over-recruitment of negative regulators by increasing nucleosome density. Finally, we divulge ongoing efforts and investigative directions toward a deeper mechanistic understanding of transcriptional regulation of circadian gene expression at the chromatin level

A New Layer Casting System for Ceramic Laser Rapid Prototyping Apparatus

In the existing Ceramic Laser Fusion system, slurry is fed on the high temperature surface of the green part; therefore, a part of water infiltrates into the green block and vaporizes before the process of layer casting. As a result, the slurry viscosity rises gradually; the quality of the layer surface is not uniform, and the green part density is uneven. The aim of present study is to develop a new layer casting system which can solve the problems mentioned above to obtain a green part with uniform surface quality and density, and to shorten the time-taken of part fabrication. The first part of the paper illustrates the major requirements and parameters of a slurry distributor; the second part describes the integration of the slurry feeding device and layer casting system. The integrated system can feed slurry and cast thin layer simultaneously; consequently, the drawbacks of the existing system can be eliminated and the time-taken of the layer casting can be shortened. A variable-frequency drive (inverter) is used to control the motor speed. The relation between the frequency and the slurry delivery can be included in the process control program to adjust the quantity in accordance with the layer thickness; hence, the waste of the slurry can be reduced.Mechanical Engineerin

Topological Phases in Neuberger-Dirac operator

The response of the Neuberger-Dirac fermion operator D=\Id + V in the topologically nontrivial background gauge field depends on the negative mass parameter $m_0$ in the Wilson-Dirac fermion operator $D_w$ which enters $D$ through the unitary operator $V = D_w (D_w^{\dagger} D_w)^{-1/2}$. We classify the topological phases of $D$ by comparing its index to the topological charge of the smooth background gauge field. An exact discrete symmetry in the topological phase diagram is proved for any gauge configurations. A formula for the index of D in each topological phase is derived by obtaining the total chiral charge of the zero modes in the exact solution of the free fermion propagator.Comment: 27 pages, Latex, 3 figures, appendix A has been revise

The Characteristics and Applications of Ceramic Laser Fusion and Ceramic Laser Sintering

The aim of present study is to investigate the possible application of the ceramic parts which are fabricated with the process of Ceramic Laser Fusion or Ceramic Laser Sintering. The experimental results reveal: (1) CLF can lead to a reduction in the porosity of the ceramic part but also can induce micro-cracks. Therefore, this process cannot produce a part with the required strength by a post-process of infiltration; (2) CLS is capable of fabricating a ceramic part with high porosity. By adjusting the slurry formulation and varying the scanning energy, the open porosity can be over 90vol% of the total porosity. After a post-process of infiltration, the density can be increased to 95%; therefore, CLS can apply to produce a part with high strength. Because the high open porosity leads to a good permeability, the process of CLS is suitable for the fabrication of ceramic shell mold.Mechanical Engineerin

Experimental and theoretical investigation for the suppression of the plasma arc drop in the thermionic converter

Ion generation and recombination mechanisms in the cesium plasma as they pertain to the advanced mode thermionic energy converter were studied. The decay of highly ionized cesium plasma was studied in the near afterglow to examine the recombination processes. Very low recombination in such a plasma may prove to be of considerable importance in practical converters. The approaches of external cesium generation were vibrationally excited nitrogen as an energy source of ionization of cesium ion, and microwave power as a means of resonant sustenance of the cesium plasma. Experimental data obtained so far show that all three techniques - i.e., the non-LTE high-voltage pulsing, the energy transfer from vibrationally excited diatomic gases, and the external pumping with a microwave resonant cavity - can produce plasmas with their densities significantly higher than the Richardson density. The implication of these findings as related to Lam's theory is discussed

Incipient ferralization and weathering indices along a soil chronosequence in Taiwan

The low hilly topography of Green Island, a volcanic island off southeastern Taiwan, includes an altitudinal sequence of sub-horizontal benches. We examined eight profiles along this sequence, ranging from pale brown loamy coral sand on the lowest bench that fringes the coast at an elevation of about 10 m to deep, intensely red and acid clay on the highest bench at about 240 m. Chemical analyses, differential Fe extractions, thin sections, X-ray diffraction of the clay minerals and indices of pedochemical weathering and strain indicated that soil development progressed by weathering of primary and secondary phyllosilicates through argilluviation in the intermediate stages to the generation of increasing quantities of free Fe. The Fe accumulates as free sesquioxides, which crystallize with age. Taxonomically the soil types progress from sandy coral Arenosol, through Eutric Cambisol, Hypereutric Lixisol and Acrisol to incipient Ferralsol (Udipsamment → Eutrudept → Udalf → Udultisol → Udox in Soil Taxonomy). The profiles are interpreted as a chronosequence, although this is complicated by minor and upwardly diminishing contributions of reef coral to the mainly igneous parent materials. There are also variations in the andesitic-basaltic bedrock, and minor aeolian inputs in the higher and older soil types. Regional eustatic sea-level correlations, 14C dating of carbonates on the two lowest benches and estimates of local tectonic uplift indicate that the incipient Ferralsols on the upper bench might date from about 150 ka. The transition through argilluvial Acrisols to incipient sesquioxide-dominated Ferralsols appears, therefore, to develop within 100–200 ka on Green Island, which is faster than usual