Fast method for the determination of short-chain-length polyhydroxyalkanoates (scl-PHAs) in bacterial samples by In Vial-Thermolysis (IVT)

none8siA new method based on the GC–MS analysis of thermolysis products obtained by treating bacterial samples at a high temperature (above 270 C) has been developed. This method, here named “In-Vial- Thermolysis” (IVT), allowed for the simultaneous determination of short-chain-length polyhydrox- yalkanoates (scl-PHA) content and composition. The method was applied to both single strains and microbial mixed cultures (MMC) fed with different carbon sources. The IVT procedure provided similar analytical performances compared to previous Py-GC–MS and Py- GC-FID methods, suggesting a similar application for PHA quantitation in bacterial cells. Results from the IVT procedure and the traditional methanolysis method were compared; the correlation between the two datasets was fit for the purpose, giving a R2 of 0.975. In search of further simplification, the rationale of IVT was exploited for the development of a “field method” based on the titration of thermolyzed samples with sodium hydrogen carbonate to quantify PHA inside bacterial cells. The accuracy of the IVT method was fit for the purpose. These results lead to the possibility for the on-line measurement of PHA productivity. Moreover, they allow for the fast and inexpensive quantification/characterization of PHA for biotechnological process control, as well as investigation over various bacterial communities and/or feeding strategies.mixedF. Abbondanzi; G. Biscaro; G. Carvalho; L. Favaro; P. Lemos; M. Paglione; C. Samorì; C. TorriF. Abbondanzi; G. Biscaro; G. Carvalho; L. Favaro; P. Lemos; M. Paglione; C. Samorì; C. Torr

Connecting Disciplines and Tracing an Educated Imagination: Biennale of Sydney Pavilions Design Summer Studio

In January 2010 the Architecture Program of the Faculty of the Built Environment at the UNSW hosted a design summer studio: 'Biennale of Sydney Pavilions' open to approximately thirty Masters students of architecture and fine arts. The studio took place twice a week for four weeks with a total of 48 hours. The purpose of this studio was to give the students the opportunity of designing a pavilion for the 17th Biennale of Sydney visitors, already affected by the display of many artworks in the Biennale, with specific spaces limited to contemplation, thinking and meditation. The pavilion, intended as the point of interaction between art, architecture and the natural beauty of the Sydney Harbour, would offer to Biennale visitors a moment for pause and reflection. The aim of the studio was to cultivate in the students an 'educated design imagination' through the integration of multiple disciplines in order to approach the design in a holistic way. Accordingly, the disciplinary background of the four lecturers/tutors involved in this studio included Art, Architecture and Philosophical Aesthetics. The paper traces the vital role of these respective disciplines taught in the design studio and attempts to gauge to what extent the students will benefit from this multidisciplinary exposure. The term 'educated imagination' is borrowed from the Canadian scholar Northrop Frye's book The Educated Imagination, (1963)1, where he distinguishes the way the sciences and the arts construct imagination from opposite ends. Frye suggests that science begins with the world as it is and from a rational and intellectual approach science turns to imagination. On the other hand, 'art begins with the world we construct, not with the world we see. It starts with the imagination, and then works towards ordinary experience'

An Inquiry into the Design and Aesthetics of the Venice Biennale Pavilions

Margreiter's film Pavilion and McQueen's film Giardini exhibited respectively inside the Austrian Pavilion and the British Pavilion at the 53rd Venice Biennale of Art, question the architecture of the pavilion in general in the constructed environment of the Giardini. Is the architecture used as a container of art or the architectural form, the container itself, to be interpreted as art? Their films are about the places in which they are shot and displayed: the pavilions themselves, the containers of art, which are transformed through the films into architectural sculpture/art objects. Arguably, Margreiter's and McQueen's film reveal the extent to which the modern language of architecture of the Venice pavilions, frozen in 'space' (within the boundary of the Giardini) and 'time' (still contemporary from the date of their realization), seem to conjure that particular primordial 'timelessness' which can be valued as one of the main attributes of contemporary architecture in general. These 'kaleidoscopic spaces' which are used to haunt our memory are used to stimulate the ontological role of imagination - creating a new experience of the universe via the pavilion

Melhoramento genético de microrganismos para produção de biocombustíveis e químicos renováveis: integração de abordagens de biologia molecular, biologia sistêmica, biologia sintética e engenharia metabólica.

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Sistema coletor de frutos de macaúba.

Este trabalho teve como objetivo avaliar a qualidade do óleo na polpa de frutos de macaúba em função do tempo de permanência de frutos no campo mantido em sistema de coletores/contentores

Características químicas e bioquímicas de frutos de macaúbas: maturação uniforme no cacho.

Para contribuir com conhecimento sobre a maturação de frutos de macauba, neste trabalho foram investigados aspectos de composição e atividade enzimática em frutos de macaúba dispostos nas porções apical, mediana e distal do cacho

TRAIL-induced variation of cell signaling states provides nonheritable resistance to apoptosis.

TNFα-related apoptosis-inducing ligand (TRAIL), specifically initiates programmed cell death, but often fails to eradicate all cells, making it an ineffective therapy for cancer. This fractional killing is linked to cellular variation that bulk assays cannot capture. Here, we quantify the diversity in cellular signaling responses to TRAIL, linking it to apoptotic frequency across numerous cell systems with single-cell mass cytometry (CyTOF). Although all cells respond to TRAIL, a variable fraction persists without apoptotic progression. This cell-specific behavior is nonheritable where both the TRAIL-induced signaling responses and frequency of apoptotic resistance remain unaffected by prior exposure. The diversity of signaling states upon exposure is correlated to TRAIL resistance. Concomitantly, constricting the variation in signaling response with kinase inhibitors proportionally decreases TRAIL resistance. Simultaneously, TRAIL-induced de novo translation in resistant cells, when blocked by cycloheximide, abrogated all TRAIL resistance. This work highlights how cell signaling diversity, and subsequent translation response, relates to nonheritable fractional escape from TRAIL-induced apoptosis. This refined view of TRAIL resistance provides new avenues to study death ligands in general

Fatty acids profile and thermal properties of macauba palm pulp oil from Cerrados and Pantanal biomes, Brazil.

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