Can a Multifaceted Intervention Including Motivational Interviewing Improve Medication Adherence, Quality of Life, and Mortality Rates in Older Patients Undergoing Coronary Artery Bypass Surgery? A Multicenter, Randomized Controlled Trial with 18-Month Follow-Up.

BACKGROUND: Patients undergoing coronary artery bypass graft (CABG) surgery are required to take a complex regimen of medications for extended periods, and they may have negative outcomes because they struggle to adhere to this regimen. Designing effective interventions to promote medication adherence in this patient group is therefore important. OBJECTIVE: The present study aimed to evaluate the long-term effects of a multifaceted intervention (psycho-education, motivational interviewing, and short message services) on medication adherence, quality of life (QoL), and mortality rates in older patients undergoing CABG surgery. METHODS: Patients aged over 65 years from 12 centers were assigned to the intervention (EXP; n = 144) or treatment-as-usual (TAU; n = 144) groups using cluster randomization at center level. Medication adherence was evaluated using the Medication Adherence Rating Scale (MARS), pharmacy refill rate, and lipid profile; QoL was evaluated using Short Form-36. Data were collected at baseline; 3, 6, and 18 months after intervention. Survival status was followed up at 18 months. Multi-level regressions and survival analyses for hazard ratio (HR) were used for analyses. RESULTS: Compared with patients who received TAU, the MARS, pharmacy refill rate, and lipid profile of patients in the EXP group improved 6 months after surgery (p < 0.01) and remained so 18 months after surgery (p < 0.01). QoL also increased among patients in the EXP group as compared with those who received TAU at 18 months post-surgery (physical component summary score p = 0.02; mental component summary score p = 0.04). HR in the EXP group compared with the TAU group was 0.38 (p = 0.04). CONCLUSION: The findings suggest that a multifaceted intervention can improve medication adherence in older patients undergoing CABG surgery, with these improvements being maintained after 18 months. QoL and survival rates increased as a function of better medication adherence. ClinicalTrials.gov NCT02109523

The role of peer physical activity champions in the workplace: a qualitative study

Aims: Peer health champions are suggested as an important component of multilevel workplace interventions to promote healthy behaviours such as physical activity. There is accumulating quantitative evidence of their effectiveness but as yet little exploration of why and how champions influence the behaviour of their peers. The current study explores the role of peer physical activity champions (PPACs) in influencing colleagues’ physical activity behaviour from the perspectives of both champions and colleagues. Methods: Seven months after the introduction of a workplace physical activity programme in 17 small and medium sized enterprices (SMEs) two focus groups were held with PPACs and four with programme participants. Focus groups were semi-structured and topics covered included: the influence of PPACs and other colleagues on their physical activity, characteristics of an effective PPAC and feelings about the PPAC role. Data were analysed using inductive thematic analysis. Results: Three overarching themes emerged: how PPACs encourage physical activity; valuable PPAC characteristics; and sustaining motivation for the PPAC role. Both direct encouragement from PPACs and facilitation of wider physical activity supportive social networks within the workplace encouraged behaviour change. Physical activity behaviour change is a delicate subject and it was important that PPACs provided enthusiastic and persistent encouragement without seeming judgemental. Being a physical activity role model was also a valuable characteristic. The PPACs found it satisfying to see positive changes in their colleagues who had become more active. However, colleagues often did not engage in suggested activities and PPACs required resilience to maintain personal motivation for the role despite this. Conclusions: The results indicate that it is feasible to incorporate PPACs into SME based physical activity interventions. Given the importance that participants attached to feeling part of a group of individuals with a common aim of increasing their physical activity, it is recommended that PPAC training includes suggestions for facilitating social connections between colleagues. Sensitivity is required when initiating and engaging in conversations with colleagues about increasing their physical activity and therefore brief motivational interviewing training may be helpful for PPACs. Programmes should ensure PPACs themselves are provided with social support, especially from others in the same role, to help sustain motivation for their role. These findings will be useful to health-promotion professionals developing workplace health programmes. Future research should explore the processes by which peer health champions facilitate changes in a range of health behaviours to identify common and behaviour specific recommendations

Globally convergent evolution strategies for constrained optimization

International audienceIn this paper we propose, analyze, and test algorithms for constrained optimization when no use of derivatives of the objective function is made. The proposed methodology is built upon the globally convergent evolution strategies previously introduced by the authors for unconstrained optimization. Two approaches are encompassed to handle the constraints. In a first approach, feasibility is first enforced by a barrier function and the objective function is then evaluated directly at the feasible generated points. A second approach projects first all the generated points onto the feasible domain before evaluating the objective function.The resulting algorithms enjoy favorable global convergence properties (convergence to stationarity from arbitrary starting points), regardless of the linearity of the constraints.The algorithmic implementation (i) includes a step where previously evaluated points are used to accelerate the search (by minimizing quadratic models) and (ii) addresses the particular cases of bounds on the variables and linear constraints. Our solver is compared to others, and the numerical results confirm its competitiveness in terms of efficiency and robustness

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

Background: Management of influenza, a major contributor to the worldwide disease burden, is complicated by lack of reliable methods for early identification of susceptible individuals. Identification of molecular markers that can augment existing diagnostic tools for prediction of severity can be expected to greatly improve disease management capabilities. Methodology/Principal Findings: We have analyzed cytokines, proteome flux and protein adducts in bronchoalveolar lavage (BAL) and sera from mice infected with influenza A virus (PR8 strain) using a previously established non-lethal model of influenza infection. Through detailed cytokine and protein adduct measurements of murine BAL, we first established the temporal profile of innate and adaptive responses as well as macrophage and neutrophil activities in response to influenza infection. A similar analysis was also performed with sera from a longitudinal cohort of influenza patients. We then used an iTRAQ-based, comparative serum proteome analysis to catalog the proteome flux in the murine BAL during the stages correlating with “peak viremia,” “inflammatory damage,” as well as the “recovery phase.” In addition to activation of acute phase responses, a distinct class of lung proteins including surfactant proteins was found to be depleted from the BAL coincident with their “appearance” in the serum, presumably due to leakage of the protein following loss of the integrity of the lung/epithelial barrier. Serum levels of at least two of these proteins were elevated in influenza patients during the febrile phase of infection compared to healthy controls or to the same patients at convalescence. Conclusions/Significance: The findings from this study provide a molecular description of disease progression in a mouse model of influenza and demonstrate its potential for translation into a novel class of markers for measurement of acute lung injury and improved case management.Singapore. National Research FoundationSingapore-MIT Alliance for Research and Technology (ID-IRG research program

Tracking of dietary intakes in early childhood : the Melbourne InFANT program

Background/Objectives: The objectives of the present study were to describe food and nutrient intakes in children aged 9 and 18 months, and to assess tracking of intakes between these two ages.Subjects/Methods: Participants were 177 children of first-time mothers from the control arm of the Melbourne Infant Feeding Activity and Nutrition Trial (InFANT) Program. Dietary intake was collected at 9 and 18 months using three 24&thinsp;h diet recalls. Tracking was assessed for food and nutrient intakes using logistic regression analysis and estimating partial correlation coefficients, respectively.Results: Although overall nutrient intakes estimated in this study did not indicate a particular risk of nutrient deficiency, our findings suggest that consumption of energy-dense, nutrient-poor foods occurred as early as 9 months of age, with some of these foods tracking highly over the weaning period. Intakes of healthier foods such as fruits, vegetables, dairy products, eggs, fish and water were also relatively stable over this transition from infancy to toddlerhood, along with moderate tracking for riboflavin, iodine, fibre, calcium and iron. Tracking was low but close to &rho;=0.3 for zinc, magnesium and potassium intakes.Conclusions: The tracking of energy-dense, nutrient-poor foods has important implications for public health, given the development of early eating behaviours is likely to be modifiable. At this stage of life, dietary intakes are largely influenced by the foods parents provide, parental feeding practices and modelling. This study supports the importance of promoting healthy dietary trajectories from infancy.<br /

The Effect of a DNA Damaging Agent on Embryonic Cell Cycles of the Cnidarian Hydractinia echinata

The onset of gastrulation at the Mid-Blastula Transition can accompany profound changes in embryonic cell cycles including the introduction of gap phases and the transition from maternal to zygotic control. Studies in Xenopus and Drosophila embryos have also found that cell cycles respond to DNA damage differently before and after MBT (or its equivalent, MZT, in Drosophila). DNA checkpoints are absent in Xenopus cleavage cycles but are acquired during MBT. Drosophila cleavage nuclei enter an abortive mitosis in the presence of DNA damage whereas post-MZT cells delay the entry into mitosis. Despite attributes that render them workhorses of embryonic cell cycle studies, Xenopus and Drosophila are hardly representative of diverse animal forms that exist. To investigate developmental changes in DNA damage responses in a distant phylum, I studied the effect of an alkylating agent, Methyl Methanesulfonate (MMS), on embryos of Hydractinia echinata. Hydractinia embryos are found to differ from Xenopus embryos in the ability to respond to a DNA damaging agent in early cleavage but are similar to Xenopus and Drosophila embryos in acquiring stronger DNA damage responses and greater resistance to killing by MMS after the onset of gastrulation. This represents the first study of DNA damage responses in the phylum Cnidaria

Desmoglein 2 is a substrate of kallikrein 7 in pancreatic cancer

<p>Abstract</p> <p>Background</p> <p>In a previous report we have demonstrated that the chymotryptic-like serine protease kallikrein 7 (<it>KLK7</it>/hK7) is overexpressed in pancreatic cancer. In normal skin, hK7 is thought to participate in skin desquamation by contributing in the degradation of desmosomal components, such as desmogleins. Thus, the ability of hK7 to degrade desmogleins was assessed and the effect of hK7 expression on desmoglein 2 was examined in cultured pancreatic cancer cells.</p> <p>Methods</p> <p>The expression of Dsg1, Dsg2, and Dsg3 in pancreatic tissues was examined by immunohistochemistry and their expression in two pancreatic cancer cell lines, BxPC-3 and Panc-1, was determined by western blot analysis. The ability of hK7 to degrade Dsg1 and Dsg2 was investigated using <it>in vitro </it>degradation assays. BxPC-3 cells stably transfected to overexpress hK7 were used to examine the effect of hK7 on cell-surface resident Dsg2.</p> <p>Results</p> <p>The levels of immunoreactive Dsg1 and Dsg2 were reduced in pancreatic adenocarcinomas compared with both normal pancreatic and chronic pancreatitis tissues. Among the desmosomal proteins examined, Dsg2 exhibited robust expression on the surface of BxPC-3 cells. When hK7 was overexpressed in this cell line, there was a significant increase in the amount of soluble Dsg2 released into the culture medium compared with vector-transfected control cells.</p> <p>Conclusion</p> <p>A reduction in the amount of the cell adhesion components Dsg1 and Dsg2 in pancreatic tumors suggests that loss of these desmosomal proteins may play a role in pancreatic cancer invasion. Using <it>in vitro </it>degradation assays, both Dsg1 and Dsg2 could be readily proteolyzed by hK7, which is overexpressed in pancreatic adenocarcinomas. The enforced expression of hK7 in BxPC-3 cells that express significant amounts of Dsg2 resulted in a marked increase in the shedding of soluble Dsg2, which is consistent with the notion that aberrant expression of hK7 in pancreatic tumors may result in diminished cell-cell adhesion and facilitate tumor cell invasion.</p

Healthcare workers' participation in a healthy-lifestyle-promotion project in western Sweden

<p>Abstract</p> <p>Background</p> <p>Healthcare professionals play a central role in health promotion and lifestyle information towards patients as well as towards the general population, and it has been shown that own lifestyle habits can influence attitudes and counselling practice towards patients. The purpose of this study was to explore the participation of healthcare workers (HCWs) in a worksite health promotion (WHP) programme. We also aimed to find out whether HCWs with poorer lifestyle-related health engage in health-promotion activities to a larger extent than employees reporting healthier lifestyles.</p> <p>Method</p> <p>A biennial questionnaire survey was used in this study, and it was originally posted to employees in the public healthcare sector in western Sweden, one year before the onset of the WHP programme. The response rate was 61% (n = 3207). In the four-year follow-up, a question regarding participation in a three-year-long WHP programme was included, and those responding to this question were included in the final analysis (n = 1859). The WHP programme used a broad all-inclusive approach, relying on the individual's decision to participate in activities related to four different themes: physical activity, nutrition, sleep, and happiness/enjoyment.</p> <p>Results</p> <p>The participation rate was around 21%, the most popular theme being physical activity. Indicators of lifestyle-related health/behaviour for each theme were used, and regression analysis showed that individuals who were sedentary prior to the programme were less likely to participate in the programme's physical activities than the more active individuals. Participation in the other three themes was not significantly predicted by the indicators of the lifestyle-related health, (body mass index, sleep disturbances, or depressive mood).</p> <p>Conclusion</p> <p>Our results indicate that HCWs are not more prone to participate in WHP programmes compared to what has been reported for other working populations, and despite a supposedly good knowledge of health-related issues, HCWs reporting relatively unfavourable lifestyles are not more motivated to participate. As HCWs are key actors in promoting healthy lifestyles to other groups (such as patients), it is of utmost importance to find strategies to engage this professional group in activities that promote their own health.</p

A genome scan for quantitative trait loci affecting cyanogenic potential of cassava root in an outbred population

<p>Abstract</p> <p>Background</p> <p>Cassava (<it>Manihot esculenta </it>Crantz) can produce cyanide, a toxic compound, without self-injury. That ability was called the cyanogenic potential (CN). This project aimed to identify quantitative trait loci (QTL) associated with the CN in an outbred population derived from 'Hanatee' × 'Huay Bong 60', two contrasting cultivars. CN was evaluated in 2008 and in 2009 at Rayong province, and in 2009 at Lop Buri province, Thailand. CN was measured using a picrate paper kit. QTL analysis affecting CN was performed with 303 SSR markers.</p> <p>Results</p> <p>The phenotypic values showed continuous variation with transgressive segregation events with more (115 ppm) and less CN (15 ppm) than either parent ('Hanatee' had 33 ppm and 'Huay Bong 60' had 95 ppm). The linkage map consisted of 303 SSR markers, on 27 linkage groups with a map that encompassed 1,328 cM. The average marker interval was 5.8 cM. Five QTL underlying CN were detected. <it>CN08R1</it>from 2008 at Rayong, <it>CN09R1</it>and <it>CN09R2 </it>from 2009 at Rayong, and <it>CN09L1 </it>and <it>CN09L2 </it>from 2009 at Lop Buri were mapped on linkage group 2, 5, 10 and 11, respectively. Among all the identified QTL, <it>CN09R1 </it>was the most significantly associated with the CN trait with LOD score 5.75 and explained the greatest percentage of phenotypic variation (%Expl.) of 26%.</p> <p>Conclusions</p> <p>Five new QTL affecting CN were successfully identified from 4 linkage groups. Discovery of these QTL can provide useful markers to assist in cassava breeding and studying genes affecting the trait.</p

Composition, potential emissions and agricultural value of pig slurry from Spanish commercial farms

[EN] Pig slurry is a valuable fertilizer for crop production but at the same time its management may pose environmental risks. Slurry samples were collected from 77 commercial farms of four animal categories (gestating and lactating sows, nursery piglets and growing pigs) and analyzed for macronutrients, micronutrients, heavy metals and volatile fatty acids. Emissions of ammonia (NH3) and biochemical methane potential (BMP) were quantified. Slurry electrical conductivity, pH, dry matter content and ash content were also determined. Data analysis included an analysis of correlations among variables, the development of predictionmodels for gaseousemissions and the analysis of nutritional content of slurries for crop production. Descriptive information is provided in this work and shows a wide range of variability in all studied variables. Animal category affected some physicochemical parameters, probably as a consequence of different slurry management and use of cleaning water. Slurries from gestating sows and growing pigs tended to be more concentrated in nutrients, whereas the slurry from lactating sows and nursery piglets tended to be more diluted. Relevant relationships were found among slurry characteristics expressed in fresh basis and gas emissions. Predictivemodels using on-farmmeasurable parameterswere obtained forNH3 (R2 = 0.51) andCH4 (R2 = 0.76), which suggests that BMP may be estimated in commercial farms from easily determined slurry characteristics. Finally, slurry nutrient composition was highly variable. Therefore, complete analyses of slurries should be performed for an effective and environmental friendly land application.This project was funded by the Spanish Ministry of Science and Innovation (AGL2011-30023) and the Valencian Government (ACOMP/2013/118). We thank the BABEL Project, Building Academic Bonds between Europe and Latin America. Erasmus Mundus Programme Action 2 for PhD fellowships. The translation of this paper was funded by the Universitat Politecnica de Valencia, Spain.Antezana-Julian, WO.; Blas, CD.; García-Rebollar, P.; Rodríguez, C.; Beccaccia, A.; Ferrer Riera, P.; Cerisuelo, A.... (2016). Composition, potential emissions and agricultural value of pig slurry from Spanish commercial farms. Nutrient Cycling in Agroecosystems. 104(2):159-173. https://doi.org/10.1007/s10705-016-9764-3S1591731042Aarnink AJA, Verstegen MWA (2007) Nutrition, key factor to reduce environmental load from pig production. Livest Sci 109(1–3):194–203Abubaker J, Risberg K, Jönsson E, Dahlin A S, Cederlund H, Pell M (2015) Short-term effects of biogas digestates and pig slurry application on soil microbial activity. Appl Environ Soil Sci. Article ID 658542: 1–15Adekunle KF, Okolie JA (2015) A review of biochemical process of anaerobic digestion. Adv Biosci Biotechnol 6:205–212Angelidaki I, Alves M, Bolzonella D, Borzacconi L, Campos JL, Guwy J, Kalyuzhnyi S, Jenicek P, Van Lier JB (2009) Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. Water Sci Technol 59(5):927–934Antezana W, Calvet S, Beccaccia A, Ferrer P, De Blas C, García-Rebollar P, Cerisuelo A (2015) Effects of nutrition on digestion efficiency and gaseous emissions from slurry in growing pigs: III. Influence of varying the dietary level of calcium soap of palm fatty acids distillate with or without orange pulp supplementation. Anim Feed Sci Technol 209:128–136APHA (2005) Standard methods for the examination of water and wastewater. Centennial, BaltimoreBai ZH, Qin W, Chen Q, Oenema O, Zhang FS (2014) Changes in pig production in china and their effects on nitrogen and phosphorus use and losses. Environ Sci Technol 48:12742–12749Beccaccia A, Ferrer P, Ibáñez MA, Estellés F, Rodríguez C, Moset V, De Blas C, Calvet S, García-rebollar P (2015) Relationships among slurry characteristics and gaseous emissions at different types of commercial spanish pig farms. Span J Agric Res 13(1):1–15Conn KL, Topp E, Lazarovits G (2007) Factors influencing the concentration of volatile fatty acids, ammonia, and other nutrients in stored liquid pig manure. J Environ Qual 36(2):440–447Diacono M, Montemurro F (2010) Long-term effects of organic amendments on soil fertility. A review. Agron Sustain Dev 30:401–422. doi: 10.1051/agro/2009040Díez JA, Hernaiz P, Muñoz MJ, Torre A, Vallejo A (2006) Impact of pig slurry on soil properties, water salinization, nitrate leaching and crop yield in a four-year experiment in Central Spain. Soil Use Manag 20(4):444–450Dourmad J-Y, Jondreville C (2007) Impact of nutrition on nitrogen, phosphorus, Cu and Zn in pig manure, and on emissions of ammonia and odours. Livest Sci 112(3):192–198EUROSTAT (2015) Pig farming sector—statistical portrait. Pig Farming in the European Union: considerable variations from one member state to another. Statistics in Focus 15/2014. Author: Pol Marquer, Teresa Rabade, Roberta Forti ISSN:2314-9647, Catalogue number: KS-SF-14-015-EN-NFangueiro D, Lopes C, Surgy S, Vasconcelos E (2012a) Effect of the pig slurry separation techniques on the characteristics and potential availability of N to plants in the resulting liquid and solid fractions. Biosyst Eng 113(2):187–194Fangueiro D, Ribeiro H, Vasconcelos E, Coutinho J, Cabral F (2012b) Influence of animal slurries composition and relative particle size fractions on the C and N mineralization following soil incorporation. Biomass Bioenergy 47:50–51Ferrer P, Cambra-López M, Cerisuelo A, Peñaranda D, Moset V (2014) The use of agricultural substrates to improve methane yield in anaerobic co-digestion with pig slurry: effect of substrate type and inclusion level. Waste Manag 34:196–203Galassi G, Colombini S, Malagutti L, Crovetto GM, Rapetti L (2010) Effects of high fibre and low protein diets on performance, digestibility, nitrogen excretion and ammonia emission in the heavy pig. Anim Feed Sci Technol 161:3–4Gopalan P, Jensen PD, Batstone DJ (2013) Anaerobic digestion of swine effluent: impact of production stages. Biomass Bioenergy 48:121–129Hernández D, Fernández JM, Plaza C, Polo A (2007) Water-soluble organic matter and biological activity of a degraded soil amended with pig slurry. Sci Total Environ 378:101–103Hernández D, Polo A, Plaza C (2013) Long-term effects of pig slurry on barley yield and N use efficiency under semiarid mediterranean conditions. Eur J Agron 44:47–86Hristov AN, Oh J, Lee C, Meinen R, Montes F, Ott T, Firkins J, Rotz A, Dell C, Adesogan A, Yang W, Tricarico J, Kebreab E, Waghorn G, Dijkstra J, Oosting S (2013) Mitigation of greenhouse gas emissions in livestock production—A review of technical options for non-CO2 emissions. In: Gerber PJ, Henderson B, Makkar HPS (eds) FAO Animal Production and Health Paper No. 177. FAO, RomeIguácel F, Yagüe MR, Betrán J, Orús F (2011) Ensayos de Fertilización Con Purín Porcino, En Cereales de Invierno de Secano, Dirección General de Desarrollo Rural, Centro de Transferencia Agroalimentaria, Gobierno de Aragón. Informe Técnico 226:15Jarret G, Cerisuelo A, Peu P, Martinez J, Dourmad JY (2012) Impact of pig diets with different fibre contents on the composition of excreta and their gaseous emissions and anaerobic digestion. Agric Ecosyst Environ 160:51–58Jouany JP (1982) Volatile fatty acid and alcohol determination in digestive contents, silage juices, bacterial cultures and anaerobic fermentor contents. Sci Alimen 2:131–144Krupa SV (2003) Effects of atmospheric ammonia (NH3) on terrestrial vegetation: a review. Environ Pollut 124(2):179–221Mantovi P, Fumagalli L, Beretta GP, Guermandi M (2006) Nitrate leaching through the unsaturated zone following pig slurry applications. J Hydrol 316:1–4Martínez-Suller L, Azzellino A, Provolo G (2008) Analysis of livestock slurries from farms across Northern Italy: relationship between indicators and nutrient content. Biosyst Eng 99(4):540–552Moral R, Moreno-Caselles J, Perez-Murcia MD, Perez-Espinosa A, Rufete B, Paredes C (2005a) Characterisation of the organic matter pool in manures. Bioresour Technol 96(2):153–158Moral R, Perez-Murcia MD, Perez-Espinosa A, Moreno-Caselles J, Paredes C (2005b) Estimation of nutrient values of pig slurries in Southeast Spain using easily determined properties. Waste Manag 25(7):719–725Moral R, Perez-Murcia MD, Perez-Espinosa A, Moreno-Caselles J, Paredes C, Rufete B (2008) Salinity, organic content, micronutrients and heavy metals in pig slurries from South-Eastern Spain. Waste Manag 28(2):367–371Morazán H, Alvarez-Rodriguez J, Seradj AR, Balcells J, Babot D (2015) Trade-offs among growth performance, nutrient digestion and carcass traits when feeding low protein and/or high neutral-detergent fiber diets to growing-finishing pigs. Anim Feed Sci Technol 207:168–180Moset V, Cambra-López M, Estellés F, Torres AG, Cerisuelo A (2012) Evolution of chemical composition and gas emissions from aged pig slurry during outdoor storage with and without prior solid separation. Biosyst Eng 111(1):2–10Ndegwa PM, Vaddella VK, Hristov N, Joo HS (2009) Measuring concentrations of ammonia in ambient air or exhaust air stream using acid traps.  J Environ Qual 38(2):647–653Nicholson FA, Chambers BJ, Williams JR, Unwin RJ (1999) Heavy metal contents of livestock feeds and animal manures in England and Wales. Bioresour Technol 70(1):23–31Olusegun OS (2014) Influence of NPK 15-15-15 Fertilizer and Pig Manure on Nutrient Dynamics and Production of Cowpea, Vigna unguiculata L. Walp. Am J Agric For 2(6):267Parera i Pous J, Olivé D, Mallol Nabot C, Torrijos NC (2010) Adaptación Del Uso de La Conductividad Eléctrica (CE) Para Determinar de Forma Rápida El Contenido En Nutrientes Del Purín Porcino En Catalunya. International Workshop on Anaerobic Digestion of Slaughterhouse Waste, pp 67–76Patience JF, Gould SA, Koehler D, Corrigan B, Elsbernd A, Holloway CL (2015) Super-dosed phytase improves rate and efficiency of gain in nursery pigs. Anim Ind Rep AS 661:98Penha HG, Menezes JF, Silva CA, Lopes G, Carvalho CA, Ramos SJ, Guilherme LRG (2015) Nutrient accumulation and availability and crop yields following long-term application of pig slurry in a Brazilian Cerrado soil. Nutr Cycl Agroecosyst 101(2):259–269Popovic O, Jensen LS (2012) Storage temperature affects distribution of carbon, VFA, ammonia, phosphorus, copper and zinc in raw pig slurry and its separated liquid fraction. Water Res 46(12):3849–3858Provolo G, Martínez-Suller L (2007) In situ determination of slurry nutrient content by electrical conductivity. Bioresour Technol 98(17):3235–3242Sánchez M, González JL (2005) The fertilizer value of pig slurry. I. Values depending on the type of operation. Bioresour Technol 96(10):1117–1123SAS Institute (2008) SAS/STAT User´s guide, v 9.3. SAS Institute Inc., CarySchoumans OF, Chardon WJ, Bechmann ME, Gascuel-Odoux C, Hofman G, Kronvang B, Rubæk HG, Ulén B, Dorioz JM (2014) Mitigation options to reduce phosphorus losses from the agricultural sector and improve surface water quality: a review. Sci Total Environ 468–469:1255–1266Schröder JJ, Cordell D, Smit AL, Rosemarin A (2010) Sustainable use of phosphorus. Plant Research International Wageningen UR. Report 357Scotford IM, Cumby TR, White RP, Carton OT, Lorenz F, Hatterman U, Provolo G (1998) Estimation of the nutrient value of agricultural slurries by measurement of physical and chemical properties. J Agric Eng Res 71(3):291–305Snoek DJW, Johannes DS, Ogink NWM, Koerkamp PWGG (2014) Sensitivity analysis of mechanistic models for estimating ammonia emission from dairy cow urine puddles. Biosyst Eng 121:12–24Suresh A, Choi HL, Oh DI, Moon OK (2009) Prediction of the nutrients value and biochemical characteristics of swine slurry by measurement of EC—electrical conductivity. Bioresour Technol 100:4683–4689Thygesen O, Triolo JM, Sommer SG (2012) Indicators of physical properties and plant nutrient content of animal slurry and separated slurry. Biol Eng Trans 5(3):123–135Triolo JM, Sommer SG, Møller HB, Weisbjerg MR, Jiang XY (2011) A new algorithm to characterize biodegradability of biomass during anaerobic digestion: influence of lignin concentration on methane production potential. Bioresour Technol 102:9395–9402Van Duivenbooden N, de Wit CT, Van Keulen H (1996) Nitrogen, phosphorus and potassium relations in five major cereals reviewed in respect to fertilizer recommendations using simulation modelling. Fertil Res Wagening 44:37–49Viguria M, Sanz-Cobeña A, López DM, Arriaga H, Merino P (2015) Ammonia and greenhouse gases emission from impermeable covered storage and land application of cattle slurry to bare soil. Agric Ecosyst Environ 199:261–271Villamar CA, Canuta T, Belmonte M, Vidal G (2012) Characterization of swine wastewater by toxicity identification evaluation methodology (TIE). Water Air Soil Pollut 223(1):363–369Villamar CA, Rodríguez DC, López D, Peñuela G, Vidal G (2013) Effect of the generation and physical–chemical characterization of swine and dairy cattle slurries on treatment technologies. Waste Manage Res 31(8):820–828Villar MC, Petrikova V, Díaz-Raviña M, Carballas T (2004) Recycling of organic wastes in burnt soils: combined application of poultry manure and plant cultivation. Waste Manage 24(4):365–370Webb J, Menzi H, Pain BF, Misselbrook TH, Dämmgen U, Hendriks H, Döhler H (2005) Managing ammonia emissions from livestock production in Europe. Environ Pollut 135:399–406Webb J, Broomfield M, Jones S, Donovan B (2014) Ammonia and odour emissions from UK pig farms and nitrogen leaching from outdoor pig production. Sci Total Environ 470:865–875Weiland P (2010) Biogas production: current state and perspectives. Appl Microbiol Biotechnol 85:849–860Yagüe MR, Bosch-Serra AD, Boixadera J (2012) Measurement and estimation of the fertiliser value of pig slurry by physicochemical models: usefulness and constraints. Biosyst Eng 111(2):206–216Zhang W, Wei Q, Wu S, Qi D, Li W, Zuo Z, Dong R (2014) Batch anaerobic co-digestion of pig manure with dewatered sewage sludge under mesophilic conditions. Appl Energy 128:175–18