CR1 — a dispersed repeated element associated with the Cab-1 locus in tomato

Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these genes. This repetitive element, named CR1, has been characterized via sequencing, genetic mapping and hybridization to related solanaceous species. Results indicate that there are as many as 30 copies of this element in the tomato genome and that most, if not all, are found at independent loci. Sites corresponding to 12 of the repeats have been located on different regions of chromosomes 2, 4, 5, 7, 10 and 11. A 1.6 kb Pst I- Eco RI fragment from the Cab-1 locus containing the element was sequenced and found to be 75% AT-rich. No open reading frames larger than 150 bp were detected. Several imperfect inverted repeats flanked by direct repeats could be found at the ends of the element. This arrangement is reminiscent of known transposons. Southern hybridization analysis indicates that multiple copies of CR1 exist in all species of the genus Lycopersicon as well as in Solanum lycopersicoides and S. tuberosum (potato), but not in eggplant, pepper, petunia, Datura or tobacco. Melt-off experiments indicate that members of the CR1 family in the tomato genome are more closely related to one another than to homologous members in the genomes of S. lycopersicoides or S. tuberosum , suggesting some type of concerted evolution.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43418/1/11103_2004_Article_BF00014948.pd

The direct measurement of ribosomal RNA gene activity in wheat–rye hybrids

Cloned rDNA segments, from wheat (Triticum aestivum) and rye (Secale cereale), which included the entire spacer region plus parts of the flanking 18S and 26S rRNA genes, were used to assay transcripts formed in the nuclei from wheat–rye hybrids. The tetraploid triticales (× Triticosecale Wittmack) carrying a complete rye chromosome complement and a combination of wheat chromosomes, as well as wheat and rye per se, were studied. Nuclei isolated from young leaves were incubated in the presence of [32P]UTP for 10 min, after which the total RNA was isolated. Hybridization of this radioactive RNA to the cloned rDNA segments showed that the suppression of rye NOR activity, measured cytologically, can be directly related to a major reduction in rRNA synthesis.Key words: NOR suppression, RNA transcripts, isolated nuclei. </jats:p

Secondary structure of the large ribosomal subunit RNA of the moss **Funaria hygrometrica**

In this study, the complete nucleotide sequence of the large ribosomal subunit RNA of the bryophyte Funaria hygrometrica was determined. The RNA sequence, which is the first one reported for bryophytes, was used to infer a secondary structure model. It delivers the base for further evolutionary studies in this group