Role of Hsp70 ATPase Domain Intrinsic Dynamics and Sequence Evolution in Enabling its Functional Interactions with NEFs

Catalysis of ADP-ATP exchange by nucleotide exchange factors (NEFs) is central to the activity of Hsp70 molecular chaperones. Yet, the mechanism of interaction of this family of chaperones with NEFs is not well understood in the context of the sequence evolution and structural dynamics of Hsp70 ATPase domains. We studied the interactions of Hsp70 ATPase domains with four different NEFs on the basis of the evolutionary trace and co-evolution of the ATPase domain sequence, combined with elastic network modeling of the collective dynamics of the complexes. Our study reveals a subtle balance between the intrinsic (to the ATPase domain) and specific (to interactions with NEFs) mechanisms shared by the four complexes. Two classes of key residues are distinguished in the Hsp70 ATPase domain: (i) highly conserved residues, involved in nucleotide binding, which mediate, via a global hinge-bending, the ATPase domain opening irrespective of NEF binding, and (ii) not-conserved but co-evolved and highly mobile residues, engaged in specific interactions with NEFs (e.g., N57, R258, R262, E283, D285). The observed interplay between these respective intrinsic (pre-existing, structure-encoded) and specific (co-evolved, sequence-dependent) interactions provides us with insights into the allosteric dynamics and functional evolution of the modular Hsp70 ATPase domain

Do fall additions of salmon carcasses benefit food webs in experimental streams?

Research showing that salmon carcasses support the productivity and biodiversity of aquatic and riparian ecosystems has been conducted over a variety of spatial and temporal scales. In some studies, carcasses were manipulated in a single pulse or loading rate or manipulations occurred during summer and early fall, rather than simulating the natural dynamic of an extended spawning period, a gradient of loading rates, or testing carcass effects in late fall-early winter when some salmon stocks in the US Pacific Northwest spawn. To address these discrepancies, we manipulated salmon carcass biomass in 16 experimental channels located in the sunlit floodplain of the Cedar River, WA, USA between mid-September and mid-December, 2006. Total carcass loads ranged from 0–4.0 kg/m2 (0, 0.001, 0.01, 0.1, 0.5, 1.0, 2.0 and 4.0 kg/m2, n = 2 per treatment) and were added to mimic the temporal dynamic of an extended spawning period. We found little evidence that carcasses influenced primary producer biomass or fish growth; however, nutrients and some primary consumer populations increased with loading rate. These effects varied through time, however. We hypothesize that the variable effects of carcasses were a result of ambient abiotic condition, such as light, temperature and disturbance that constrained trophic response. There was some evidence to suggest peak responses for primary producers and consumers occurred at a loading rate of *1.0–2.0 kg/m2, which was similar to other experimental studies conducted during summer

Sharing of Sponsored Advertisements on Social Media: A Uses and Gratifications Perspective

Organisations are increasingly utilising social media to advertise to, and interact with, consumers. Sponsored advertisements embedded into targeted users’ social media feeds appear less invasive than standalone advertisements but, unlike organic postings, incur financial cost. Given that friends’ posts attract most attention, this research employs Uses and Gratifications theory to determine salient motivations for users’ intentions to share sponsored advertisements, framed in the tourism context. Survey data was collected (n = 487) and analysis revealed altruism, entertainment, socialising, and information seeking to be significant positive drivers of intention to share tourism-related sponsored advertisements on Facebook. Notably, information sharing was found to have a negative effect, while self-expression had no significant effect. In addition, the motivations were not found to significantly differ between males and females. This study contributes to theoretical understanding of users’ intentions to share sponsored advertisements within the social media environment and provides practical recommendations to help tourism marketers maximise reach.</p