Responses of soil C, N, and P stoichiometric ratios to N and S additions in a subtropical evergreen broad-leaved forest

Acid deposition from the emission of nitrogen (N) and sulfur (S) has become an important factor affecting the soil nutrient balance and biogeochemical cycling in terrestrial ecosystems. The average levels of N and S deposition in the rainy area of southwestern China from 2008 to 2010 were 9.5 g N m¯² y¯¹ and 19.3 g S m¯² y¯¹, respectively. External additions of N and S fertilizers combined with high levels of acid deposition may affect the soil ecological stoichiometry in the region's widely distributed subtropical evergreen broad-leaved forest. Therefore, we investigated the responses of the soil stoichiometric ratios and enzyme activities to added N (+N), added S (+S), added N and S (+NS), and a control (Ctr) in the 0-20 cm layer in an evergreen broad-leaved forest in the rainy area of southwestern China from April 2013 to April 2015. The results showed that the soil total N (TN) concentration and N/P ratio were higher and the soil organic C (SOC) concentration and C/N ratio were lower in the fertilization treatments than the Ctr, although N and S additions did not significantly alter the soil total P (TP) concentration. The +N, +S, and +NS treatments increased the soil acid phosphatase activity and reduced the soil invertase, cellulase, catalase, and polyphenol oxidase activities. The +N and +NS treatments increased the soil urease activity and reduced soil peroxidase activity. The +S treatment reduced the soil urease activity and did not alter soil peroxidase activity. N and S additions had synergistic decreasing effects on the SOC concentration, C/N ratio, and soil cellulose and catalase activities. Moreover, structural equation models identified that N and S additions regulated the SOC, TN, and TP concentrations via shifting the activities of soil enzymes and the pathways differed between N addition and S addition. In conclusion, N and S additions decreased the SOC concentration, C/N ratio, and most soil C-cycle enzyme activities and increased the TN concentration, N/P ratio, and soil acid phosphatase activity. All these results indicated that external N and S additions combined with acid deposition increased soil N concentrations and exacerbated soil C and P limitations in this forest

The additions of Nitrogen and Sulfur synergistically decrease the release of Carbon and Nitrogen from litter in a subtropical forest

Atmospheric nitrogen (N) and sulfur (S) deposition in subtropical forests has increased rapidly and the current level is very high, thus seriously affecting nutrient (e.g., N and phosphorus (P)) release from litter. However, the specific effects of S addition and its interaction with N on the release of carbon (C), N, and P from litter in subtropical evergreen broadleaved forests are unclear. Therefore, a two-year field experiment was performed using a litterbag method in a subtropical evergreen broadleaved forest in western China to examine the responses of litter decomposition and nutrient release to the control (CK), added N (+N), added S (+S), and added N and S (+NS) treatments. The results showed that the remaining litter mass, lignin, cellulose, C, N, P, and litter N/P ratio were higher, whereas the litter C/N ratio and soil pH were lower in the fertilization treatments than in CK. The annual decomposition coefficients (k-values) in the +N, +S, and +NS treatments were 0.384 ± 0.002, 0.378 ± 0.002, and 0.374 ± 0.001 year−1, respectively, which were significantly lower than the k-values in CK (0.452 ± 0.005 year−1, p < 0.05). The remaining mass, lignin, cellulose, C, and litter N/P ratio were higher, whereas the soil pH was lower in the +NS treatment than in the +N and +S. The interactive effects of N addition and S addition on the remaining litter lignin, cellulose, C, N, and P; the litter C/N, C/P, and N/P ratios; and the soil pH were significant (p < 0.05). In conclusion, the addition of N and S synergistically decreased the degradation of lignin and cellulose and the release of C and N and increased the litter N/P ratio, suggesting that external N and S inputs synergistically slowed the release of C and N from litter and exacerbated litter P limitation during decomposition in this forest

Soil cover improves soil quality in a young walnut forest in the Sichuan Basin, China

The soil quality index (SQI) is based on several key indicators and is used to assess soil quality. More than 250,000 ha of walnut saplings (Juglans regia L.) were planted in previous cropland areas in the Sichuan Basin, China, using a range of soil cover types that may affect soil quality with effects that are unclear. We investigated the effects of white film (WF), black film (BF), shade netting (SN), and maize straw (MS) soil cover types and an uncovered control type (CK) on soil chemical and biological indicators and the SQI in the 0-15 cm soil layer in a young walnut forest in the Sichuan Basin over a 27-month study period. The results showed that all soil cover types increased the soil organic matter (SOM), total potassium (TK), and available potassium (AK) concentrations (p < 0.05), whereas the total nitrogen (TN) and available nitrogen (AN) concentrations were greater only in soils covered by MS than in CK (p < 0.05). The available phosphorus concentrations were 64.1 and 193.2% greater in soils covered by BF and MS treatments, respectively, than in the CK (p < 0.05). The numbers of soil faunal groups (N) were 45.7, 36.4, 37.2, and 101.5% higher in WF, BF, SN, and MS, respectively, than in CK (p < 0.05); the individual numbers (S) were 92.3, 36.2, 100.8, and 154.5% greater in WF, BF, SN, and MS, respectively, than in CK (p < 0.05). The microbial biomass carbon (MBC) was 15.5, 32.3, 45.0, and 77.1% greater in WF, BF, SN, and MS than in CK, respectively (p < 0.05). Redundancy discriminant analysis revealed strong positive interactions between biological indicators (MBC, N, and S) and SOM, AN, and AK concentrations. SOM, TN, AK, S, and MBC were the minimum required variables for the effective assessment of the SQI. All four soil cover types led to an improved SQI (p < 0.05), and MS had the greatest effect on SOM, TN, AN, AP, N, S, MBC, and SQI (p < 0.05). In conclusion, all four soil cover types increased the SOM levels, TK, AK, and MBC concentrations, soil faunal diversity, and SQI. The MS treatment was the most cost-effective and efficient measure to improve soil fertility, ecological function, and overall soil quality in the studied walnut forest

Evaluation of cloned cells, animal model, and ATRA sensitivity of human testicular yolk sac tumor

The testicular yolk sac tumor (TYST) is the most common neoplasm originated from germ cells differentiated abnormally, a major part of pediatric malignant testicular tumors. The present study aimed at developing and validating the in vitro and vivo models of TYST and evaluating the sensitivity of TYST to treatments, by cloning human TYST cells and investigating the histology, ultra-structure, growth kinetics and expression of specific proteins of cloned cells. We found biological characteristics of cloned TYST cells were similar to the yolk sac tumor and differentiated from the columnar to glandular-like or goblet cells-like cells. Chromosomes for tumor identification in each passage met nature of the primary tumor. TYST cells were more sensitive to all-trans-retinoic acid which had significantly inhibitory effects on cell proliferation. Cisplatin induced apoptosis of TYST cells through the activation of p53 expression and down-regulation of Bcl- expression. Thus, we believe that cloned TYST cells and the animal model developed here are useful to understand the molecular mechanism of TYST cells and develop potential therapies for human TYST

Infection with blast fungus ( Magnaporthe orzyae) leads to increased expression of an arabinogalactan-protein epitope in both susceptible and resistant rice cultivars

Blast fungus (Magnaporthe orzyae) is the most serious pathogen of rice. As such, the objective of the current study was to investigate the infection process using a selected panel of anti-plant extracellular matrix antibodies, in infected leaves from three rice cultivars that display varying degrees of resistance to the pathogen; at selected time points post inoculation. An arabinogalactan-protein (AGP) epitope, recognized by the antibody JIM13, was shown to be temporally and spatially regulated during the infection, in all three cultivars. Prior to inoculation, expression of the epitope was confined to the epidermis, sclerenchyma, phloem, xylem and bundle sheath. As the infection progressed, the intensity of labeling increased in all the aforementioned cell types and spread to the mesophyll. Immunogold transmission electron microscopy, revealed this epitope to be most abundant at the plasma membrane. These data suggest that this epitope is borne on a plasma-membrane-associated AGP, which may act as a pattern recognition receptor (PRR), involved in the basal immune response to infection with blast fungus, in both resistant and susceptible rice cultivars. As such, this study represents the first report of a membrane – associated arabinogalactan-protein in rice, which once cloned, could be of utility in future breeding programs/genetic modification to improve the basal immune response to blast fungus and other pathogens, in this globally important food crop

Bone-marrow derived cells do not contribute to new beta-cells in the inflamed pancreas

The contribution of bone-marrow derived cells (BMCs) to a newly formed beta-cell population in adults is controversial. Previous studies have only used models of bone marrow transplantation from sex-mismatched donors (or other models of genetic labeling) into recipient animals that had undergone irradiation. This approach suffers from the significant shortcoming of the off-target effects of irradiation. Partial pancreatic duct ligation (PDL) is a mouse model of acute pancreatitis with a modest increase in beta-cell number. However, the possibility that recruited BMCs in the inflamed pancreas may convert into beta-cells has not been examined. Here, we used an irradiation-free model to track the fate of the BMCs from the donor mice. A ROSA-mTmG red fluorescent mouse was surgically joined to an INS1Cre knock-in mouse by parabiosis to establish a mixed circulation. PDL was then performed in the INS1Cre mice 2 weeks after parabiosis, which was one week after establishment of the stable blood chimera. The contribution of red cells from ROSA-mTmG mice to beta-cells in INS1Cre mouse was evaluated based on red fluorescence, while cell fusion was evaluated by the presence of green fluorescence in beta-cells. We did not detect any red or green insulin+ cells in the INS1Cre mice, suggesting that there was no contribution of BMCs to the newly formed beta-cells, either by direct differentiation, or by cell fusion. Thus, the contribution of BMCs to beta-cells in the inflamed pancreas should be minimal, if any