Fasciola hepatica IN BOVINES IN BRAZIL: DATA AVAILABILITY AND SPATIAL DISTRIBUTION

Fasciolosis is a disease of importance for both veterinary and public health. For the first time, georeferenced prevalence data of Fasciola hepatica in bovines were collected and mapped for the Brazilian territory and data availability was discussed. Bovine fasciolosis in Brazil is monitored on a Federal, State and Municipal level, and to improve monitoring it is essential to combine the data collected on these three levels into one dataset. Data were collected for 1032 municipalities where livers were condemned by the Federal Inspection Service (MAPA/SIF) because of the presence of F. hepatica. The information was distributed over 11 states: Espírito Santo, Goiás, Minas Gerais, Mato Grosso do Sul, Mato Grosso, Pará, Paraná, Rio de Janeiro, Rio Grande do Sul, Santa Catarina and São Paulo. The highest prevalence of fasciolosis was observed in the southern states, with disease clusters along the coast of Paraná and Santa Catarina and in Rio Grande do Sul. Also, temporal variation of the prevalence was observed. The observed prevalence and the kriged prevalence maps presented in this paper can assist both animal and human health workers in estimating the risk of infection in their state or municipality

Abstract 1846: Gene expression analysis of P-glycoprotein and LMWPTP in chronic myeloid leukemia cells treated with metformin and imatinib mesylate

Abstract Chemotherapy is an effective treatment for many tumors; however, some tumors begin to exhibit a wide range of resistance after a few cycles of chemotherapy, which is known as phenotype of resistance to multiple drugs (multidrug resistance - MDR). The MDR phenotype arises through various mechanisms, but often is associated with an increased efflux of hydrophobic cytotoxic drugs. Therefore, the search for more selective and efficient antitumor compounds in the elimination of cancer cells boosts the research in oncology area. Studies of genes and pathways that are deregulated in tumors and that are essential to their growth may provide new strategies that lead to the development of therapeutic agents that target these pathways. Previous data from our research group have shown potent toxic activity of metformin, an oral hypoglycemic used for more than 30 years for the treatment of type II diabetes, on chronic myeloid leukemia cells (CML), potentiating the effects of imatinib mesylate, the standard chemotherapy used in the treatment of CML. CML cells treated with these agents showed changes in expression of proteins associated with the MDR phenotype. In this way, this project aimed to evaluate the expression of ABCB1 (Pgp) e ACP1 (LMWPTP) by Polymerase Chain Reaction in real time (qPCR) in LCM K562 cells and Lucena before and after treatment with metformin and/or imatinib mesylate, seeking to verify the effects of metformin, alone or in combination with Imatinib mesylate in the expression of these genes in Lucena in K562 cells. We found statistically significant differences in expression between treatments compared to control and between K562 and Lucena cells. It is possible to conclude that these genes may be associated with MDR phenotype. Citation Format: Ligia P. Oliveira, Rafael G. Ferro, Ana CSS Galvão. Gene expression analysis of P-glycoprotein and LMWPTP in chronic myeloid leukemia cells treated with metformin and imatinib mesylate. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1846.</jats:p

Study of behavior of cardiac pacemaker batteries capacity in different situations of electro-electronic variations

The research analyzes issues related to events that originate the behavior of a pacemaker battery in certain electro-electronic variations. Based on idea that pacemaker has the purpose of supporting the heart rate with electrical stimuli, the existence of errors in equipment's battery can lead to reduced autonomy and restricted failures that cause it to malfunction. The research process is based on bibliographic reviews and objective is to investigate cause of failure and explain in a technical way how this error can be minimized in order to reduce likelihood of these undesirable events in the equipment's operation.</jats:p