Evaluation of three tools for assessing infection by gastrointestinal parasites in pasture-fed organic lambs

Organic meat sheep producers have a very limited array of efficient alternative drugs and limited access to synthetic drugs for controlling internal parasites. The use of targeted selective treatments, e.g., treating only the lambs that cannot cope with infection, would be of interest. The difficulty is to identify those lambs in need of treatment. FAMACHA© (based on an anaemia indicator) has been used with success in tropical areas where the bloodsucker worm Haemonchus contortus is the main gastrointestinal strongyle. The appearance of the wool is sometimes used to determine the necessity of treatment, and a spectrofluorometric analysis of the wool was tested. Shepherds may also detect lambs with poor, medium and good body conditions, on the basis of experience. In our study, the three estimators for gastrointestinal parasites were not related to parasite faecal egg or oocyste counts, which are the usual laboratory tools for assessing infection

First-principles calculations of X-ray absorption spectra at the K-edge of 3d transition metals: an electronic structure analysis of the pre-edge

International audienceWe first present an extended introduction of the various methods used to extract electronic and structural information from the K pre-edge X-ray absorption spectra of 3d transition metal ions. The K pre-edge structure is then modelled for a selection of 3d transition metal compounds and analyzed using first-principles calculations based on the density functional theory (DFT) in the local density approximation (LDA). The selected compounds under study are presented in an ascending order of electronic structure complexity, starting with the Ti K-edge of rutile and anatase, and finishing with the Fe K-edge of the cyanomet-myoglobin. In most cases, the calculations are compared to polarized experimental spectra. It is shown that DFT-LDA methods enable us to reproduce satisfactorily the experimental features and to understand the nature of the electronic transitions involved in the pre-edge region. The limiting aspects of such methods in modelling the core-hole electron interaction and the 3d electron-electron repulsion are also pointed out

Animal health strategies in organic and conventional meat sheep production

Sixteen meat sheep farms (nine conventional and seven organic) in the centre of France were surveyed to evaluate their economic and production performances (previous interviews) and their strategies related to animal health with particular attention to internal parasites (present interview on health strategies). The organic farms were surveyed in mid-September 2006 and the conventional ones in October 2007. Each interview (1½ or 2 h) included a visit of the farm (with the collection of faeces for evaluation of internal parasites), followed by an open discussion on sheep production and health problems, and parasitic infections, in particular. The discussion was recorded and then transcribed into a word processor file and analysed. The farmers agreed on the fragile health of their animals (with special attention to internal parasites)and were confronted with the low value of each lamb or ewe, which does not allow for high health costs. Even under this limited economic situation, the farmers developed different health strategies. Conventional farmers rely on systematic treatments to prevent parasitic infection; they use low-cost effective synthetic drugs and accept lamb mortality rates ranging from five to eight percent. Their practices are homogeneous, which is possibly due to frequent exchanges with colleagues or veterinarians. The healthcare strategy of organic farmers may be divided into two distinct categories: autonomous (“self-made farmer”) and “creative”. The organic self-made farmers are experienced and aim at a sustainable flock (“cruiser flock”). The creative ones do not seem to easily handle the health strategy aspect and often spend more money on healthcare than their conventional or organic self-made colleagues. Health strategy is therefore not completely dependent on the type of production - conventional versus organic - but on the farmer’s conception of life and nature. The creative farmers believe that disease is a dysfunction that may be corrected with treatments (conventional or alternative ones), whereas the self-made farmers consider health as the result of a dynamic equilibrium between the many forces that interact within a flock. The latter is somewhat similar to the idea put forth in the book “The normal and the pathological” by Canguilhem

Experimental evidence of thermal fluctuations on the X-ray absorption near-edge structure at the aluminum K-edge

After a review of temperature-dependent experimental x-ray absorption near-edge structure (XANES) and related theoretical developments, we present the Al K-edge XANES spectra of corundum and beryl for temperature ranging from 300K to 930K. These experimental results provide a first evidence of the role of thermal fluctuation in XANES at the Al K-edge especially in the pre-edge region. The study is carried out by polarized XANES measurements of single crystals. For any orientation of the sample with respect to the x-ray beam, the pre-edge peak grows and shifts to lower energy with temperature. In addition temperature induces modifications in the position and intensities of the main XANES features. First-principles DFT calculations are performed for both compounds. They show that the pre-edge peak originates from forbidden 1s to 3s transitions induced by vibrations. Three existing theoretical models are used to take vibrations into account in the absorption cross section calculations: i) an average of the XANES spectra over the thermal displacements of the absorbing atom around its equilibrium position, ii) a method based on the crude Born-Oppenheimer approximation where only the initial state is averaged over thermal displacements, iii) a convolution of the spectra obtained for the atoms at the equilibrium positions with an approximate phonon spectral function. The theoretical spectra so obtained permit to qualitatively understand the origin of the spectral modifications induced by temperature. However the correct treatment of thermal fluctuation in XANES spectroscopy requires more sophisticated theoretical tools

Inventaire des mollusques d'eau douce en Ituri (Haut-Zaïre) : conséquences sanitaires pour l'homme et le bétail

Cent soixante-dix points d'abreuvement pour le bétail ont été prospectés de juin 1986 à août 1988 en Ituri (nord-est du Zaïre) afin de déterminer les principaux mollusques d'eau douce pouvant intervenir dans la transmission des trématodoses animales et humaines. Dix-neuf espèces de gastéropodes ont été rencensées. Quatre gîtes sur dix n'hébergent pas ou très peu de mollusques. Les trois espèces les plus fréquemment rencontrées sont #Lymnaea natalensis, #Biomphalaria pfeifferi$(27,6$ (15,3 %). La distribution des deux premières espèces semble très homogène au niveau régional et ne permet pas l'établissement d'une relation avec le risque parasitaire (#Fasciola gigantica et #Schistosoma mansoni). Inversement, la répartition de #Bulinus africanus (et secondairement celle de #B. forskalii) correspond globalement à la distribution de #Schistosoma bovis chez les bovins. L'association de #Lymnaea natalensis et de #Bulinus africanus au sein des gîtes rend compte de l'association des infestations à #Fasciola gigantica et #Schistosoma bovis$ chez les bovins. (Résumé d'auteur

Influence of the 6^1S_0-6^3P_1 Resonance on Continuous Lyman-alpha Generation in Mercury

Continuous coherent radiation in the vacuum-ultraviolet at 122 nm (Lyman-alpha) can be generated using sum-frequency mixing of three fundamental laser beams in mercury vapour. One of the fundamental beams is at 254 nm wavelength, which is close to the 6^1S_0-6^3P_1 resonance in mercury. Experiments have been performed to investigate the effect of this one-photon resonance on phasematching, absorption and the nonlinear yield. The efficiency of continuous Lyman-alpha generation has been improved by a factor of 4.5.Comment: 8 pages, 7 figure

New approaches to measuring anthelminthic drug efficacy: parasitological responses of childhood schistosome infections to treatment with praziquantel

By 2020, the global health community aims to control and eliminate human helminthiases, including schistosomiasis in selected African countries, principally by preventive chemotherapy (PCT) through mass drug administration (MDA) of anthelminthics. Quantitative monitoring of anthelminthic responses is crucial for promptly detecting changes in efficacy, potentially indicative of emerging drug resistance. Statistical models offer a powerful means to delineate and compare efficacy among individuals, among groups of individuals and among populations.; We illustrate a variety of statistical frameworks that offer different levels of inference by analysing data from nine previous studies on egg counts collected from African children before and after administration of praziquantel.; We quantify responses to praziquantel as egg reduction rates (ERRs), using different frameworks to estimate ERRs among population strata, as average responses, and within strata, as individual responses. We compare our model-based average ERRs to corresponding model-free estimates, using as reference the World Health Organization (WHO) 90 % threshold of optimal efficacy. We estimate distributions of individual responses and summarize the variation among these responses as the fraction of ERRs falling below the WHO threshold.; Generic models for evaluating responses to anthelminthics deepen our understanding of variation among populations, sub-populations and individuals. We discuss the future application of statistical modelling approaches for monitoring and evaluation of PCT programmes targeting human helminthiases in the context of the WHO 2020 control and elimination goals

X-ray Absorption Near-Edge Structure calculations with pseudopotentials. Application to K-edge in diamond and alpha-quartz

We present a reciprocal-space pseudopotential scheme for calculating X-ray absorption near-edge structure (XANES) spectra. The scheme incorporates a recursive method to compute absorption cross section as a continued fraction. The continued fraction formulation of absorption is advantageous in that it permits the treatment of core-hole interaction through large supercells (hundreds of atoms). The method is compared with recently developed Bethe-Salpeter approach. The method is applied to the carbon K-edge in diamond and to the silicon and oxygen K-edges in alpha-quartz for which polarized XANES spectra were measured. Core-hole effects are investigated by varying the size of the supercell, thus leading to information similar to that obtained from cluster size analysis usually performed within multiple scattering calculations.Comment: 11 pages, 4 figure