Borrelia miyamotoi in wild rodents from four different regions of Turkey

Borrelia miyamotoi is a tick-borne zoonotic agent that causes hard tick-borne relapsing fever, an emerging disease in humans. Some small mammalian and bird species are reported to be reservoirs of B. miyamotoi. This study aims to examine Borrelia species present in rodents captured from rural areas of Turkey. Blood samples of rodents were initially screened with Borrelia 16S rRNA qPCR. The Borrelia flaB gene was subsequently amplified by conventional PCR, after which all positive samples were sequenced. Borrelia miyamotoi was observed in nine out of 536 blood samples (1.7%) collected from wild rodents. Phylogenetic analysis showed that all positive samples belonged to the European genotype clade of B. miyamotoi. PCR positivity was 5.3%, 3.7%, and 1.8% in Apodemus uralensis, Apodemus flavicollis, and Myodes glareolus, respectively. Borrelia burgdorferi sensu lato that causes Lyme borreliosis in humans could not be detected in the rodents. In this study, presence of B. miyamotoi DNA is reported for the first time in rodents in Turkey

Bursa bölgesindeki sığırlarda listeria monocytogenes antikor seroprevalansının belirlenmesi

To determine the seroprevalance of antibodies to L. monocytogenes in cattle in Bursa province of Turkey was aimed in this study. Two hundred nine, aged 1-5 years, healthy Holstein cattle were randomly selected from 6 different districts and after clinical examination blood samples were collected. The herd size (number of cattle in each herd) and the animals fed silage were recorded. Antibodies to L. monocytogenes were determined by agglutination test according to the method described by of Osebold et al. Hematological parameters (white blood cell count, circulating red blood cell count, hematocrit and differenciated cell counts) were analyzed. Antibodies to L. monocytogenes were found in 101 (48.3 %) of the 209 cattle tested. Of 209 cattle tested 54 (25.8%) had 1/50, 74 (35.4%) had 1/100, 23 (11.0%) had 1/200 and 4 had (1.9%) 1/400 agglutination titer whereas no agglutination was detected in 54 (24.88%) cattle. 53.7% (58/108) of female and 42.5% (43/101) of male cattle have been found to be seropositive. The seropositivity in silage fed cattle (57.5%, 92/160) was found statistically higher than cattle that were not feed silage (18.3%, 9/49) (p<0.001). The higher seropositivity (55.8%) was observed in cattle from large herd size (50 – 100 cattle). As a result, in this study seroprevalence of antibodies to Listeria monocytogenes in cattle in Bursa province of Turkey was found as 48.3 % and it was concluded that silage feeding is an important factor in the epidemiology of listeriosis. Larger and detailed prophylactic studies must be planned to control this zoonosis infection for animal and human healthBu çalışmada Bursa bölgesindeki sığırlarda L. monocytogenes antikor seroprevalansının belirlenmesi amaçlandı. Çalışmada Bursa bölgesinde 6 farklı ilçeden rasgele seçilen Holstein ırkı, 1-5 yaşlı, sağlıklı 209 sığırın klinik muayeneleri yapıldı ve kan örnekleri alındı. Sürüdeki hayvan sayısı (sürü boyutu) ve silajla beslenen hayvanlar kaydedildi. L. monocytogenes antikor düzeyleri Oesborn ve ark. tarafından belirtilen aglutinasyon testi kullanılarak belirlendi. Tüm sığırların hematolojik muayeneleri (total lökosit, eritrosit, hematokrit ve formül lökosit) yapıldı. L. monocytogenes antikor düzeyi 1/100 ve üzeri olanlar seropozitif olarak kabul edildi. İki yüz dokuz sığırın 101’inde (% 48.32) L. monocytogenes antikorları belirlendi. Çalışmadaki sığırların 54’ünde (% 25.8) 1/50, 74’ünde (% 35.4) 1/100, 23’ünde (% 11.0) 1/200 ve 4’ünde (% 1.9) 1/400 düzeyinde antikor titresi belirlenirken, 54 sığırda (% 24.8) antikor saptanmadı. Dişilerin % 53.7’sinin (58/108) erkeklerin % 42.5’inin (43/101) seropozitif olduğu belirlendi. Silajla beslenen sığırlarda seropozitiflik oranının (% 57.5, 92/160), silajla beslenmeyenlerden (18.3%, 9/49) istatistiki olarak (p<0.001) daha yüksek olduğu saptandı. Büyük sürülerde (50-100 sığır) seropozitiflik oranının daha yüksek (% 55.8) olduğu gözlendi. Sonuç olarak, bu çalışmada Bursa bölgesinde Listeria monocytogenes antikor seroprevalansının % 48.3 olduğu belirlendi ve listeriosisin epidemiyolojisinde silajla beslenmenin önemli bir faktör olduğu ortaya konuldu. İnsan ve hayvan sağlığı açısından önemli bir zoonoz enfeksiyon olan listeriosisle mücadele için daha geniş kapsamlı profilaktif çalışmaların yapılmasının gerekliliğine karar verildi

Current Status in Intestinal Parasitic Infections: A Reference Laboratory Results

Objective: The aim of this study was to evaluate the results of examination for intestinal parasites in fecal samples sent to our laboratory by obtaining from patients applied to the hospital because of various complaints

Investigation of Antibodies Against Listeria monocytogenes in Ram and Ewes in Ankara Province

GAZYAGCI, SERKAL/0000-0002-0043-6942WOS: 000274088000027This study was conducted on determination seroprevalance of listeriosis in sacrifial ewes and rams in Ankara. Antibody titers were detected against Listeria monocytogenes by Osebold Agglutination Test ( OAT). 112 sera of 205 were found as seropositive (%54.6) against Listeriosis. According to this study, Listeria monocytogenes was determined as a common disease in rams and ewes for sacrify in Ankara

Investigation of anti-toxoplasma gondii and anti-leishmania infantum antibodies among Sivas Kangal dogs

The present study was carried out to determine the seroprevalence of toxoplasmosis and leishmaniosis among Kangal dogs on breeding farms in Sivas, Turkey. Serum samples from 50 Kangal dogs without clinical symptoms of disease were tested for anti-Toxoplasma gondii antibodies with the Sabin Feldman Dye Test (SFDT) and for anti- Leishmania antibodies with the Indirect Fluorescent Antibody Test (IFAT). Forty- six out of the 50 dogs were seropositive for Toxoplasma gondii, with titers ranging from 1: 16 to 1: 1024. Only 1 of the 50 dogs was seropositive for leishmaniosis (titer: 1: 128) and 5 had anti- Leishmania antibodies with a titer of 1: 64, which was considered uncertain. There were no statistically significant differences in the seroprevalence of toxoplasmosis and leishmaniosis based the gender and age of the dogs (P > 0.05). In conclusion, the study revealed that Toxoplasma gondii is widely distributed in Kangal dogs. Although the seroprevalence of canine leishmaniosis among Kangal dogs was low and no clinical signs were observed, asymptomatic animals can be significant reservoirs for the spread of the disease

Reactivated and clinical Toxoplasma gondii infection in young lambs: Clinical, serological and pathological evidences

Babur, Cahit/0000-0002-6524-3260; ATMACA, HASAN TARIK/0000-0001-8379-4114WOS: 000305100900054In this study, fatal toxoplasmosis with severe neurological clinical signs was evaluated in 20 lambs of a sheep flock containing 90 Akkaraman sheep, 60 lambs and 3 rams. The clinical signs in infected lambs (n = 20) included incoordination, head shaking, tremors, shaking up, difficulty walking and subsequent death. No incidence of abortion observed in pregnant ewes. Two lambs with severe clinical signs were euthanized and necropsied following clinical and hematological examinations. Blood samples were also collected from the mothers of dead lambs and rams for Sabin-Feldman dye test to detect Toxoplasma gondii-specific antibodies. T. gondii infection was further confirmed by PCR analysis using T. gondii B1 gene specific primers. The histopathological findings included non-purulent myositis with T. gondii-like tissue cysts in the heart and skeletal musculature, severe necrotic vasculitis and multifocal necroses in the brain, liver and lungs. T. gondii immunoreactivity was present in the lungs, liver and spleen as well as tissue cyst-like structures. In differential diagnosis, Neospora caninum infection was excluded by immunohistochemical and PCR analyses. According to current literature, there has been no previous report on clinical toxoplasmosis in newborn lambs or goat kids, and the number of reports on clinical toxoplasmosis in small ruminants is limited to two adult goats with fatality following systemic toxoplasmosis. Our study indicated that toxoplasmosis occurs in lambs with severe neurological signs and subsequent death. (C) 2012 Elsevier B.V. All rights reserved.Kirikkale University Research CouncilKirikkale University [21/2006]This study is financially supported by Kirikkale University Research Council Project with 21/2006 project number. The clinical toxoplasmosis cases were presented as oral presentation at XXIVth World Buiatrics Congress, 2008 Hungary and at the 4th national Veterinary Pathology Congress, 2008, Antalya, Turkey