Vaginal microbiota changes of persistent human papillomavirus infection after cervical conization

ObjectiveWe investigated the changes in vaginal microbiota among females with persistent human papillomavirus (HPV) infection following cervical conization in Xinjiang, China.MethodsA total of 108 female participants were enrolled in the study, including 37 HPV-positive females without cervical conization (Group P1), 37 HPV-positive females after cervical conization (Group P2), and 34 HPV-negative females after cervical conization (Group N). DNA was extracted from vaginal secretions, and the V3-V4 regions of bacterial 16S rDNA were amplified and sequenced using NovaSeq technology. The diversity analysis of the bacterial microbiota was conducted using QIIME2 and R software, while the phenotypic analysis was performed with Bugbase software.ResultsLactobacillus was the predominant genus in the vaginal microbiota of women with persistent HPV infection after cervical conization in Xinjiang. Following partial cervical resection, the α-diversity of the vaginal microbiota decreased, particularly among patients who had cleared HPV. Bacterial vaginosis-associated anaerobes were common in the vaginal environment, with their relative abundance increasing in cases of persistent HPV infection. Postoperative persistent HPV infection was found to be correlated not only with pathogens linked to bacterial vaginosis but also with those associated with aerobic vaginitis. Gardnerella and Atopobium, as well as Bifidobacterium and Streptococcus, demonstrated a symbiotic synergy. Both Lactobacillus and Gardnerella exhibited negative correlations with many pathogenic bacteria. Anaerobic and biofilm formation were the most evident phenotypes in individuals with persistent HPV infection after conization.ConclusionThe vaginal microbiota of women with persistent HPV infection following cervical conization is characterized by the coexistence of Lactobacillus dominance and increased microbial diversity. Anaerobic bacteria and biofilm formation may play a significant role in the persistence of HPV infection post-surgery, and the role of Gardnerella in the vaginal flora under an HPV-infected state warrants further study

Effects of Different Postharvest Treatments on Browning, Active Oxygen Metabolism and Saccharides of Gongcheng Persimmon

In this paper, the effects of three postharvest treatments (aerobic,vacuum,vacuum+1-MCP) on browning, active oxygen metabolism and saccharides during storage of deastringent persimmon were researched. Meanwhile, the correlations between browning and other indexes were analyzed. BI of vacuum and vacuum+1-MCP was lower than that in aerobic group, and the browning degree in vacuum+1-MCP group was the lowest. The SOD and APX activities of the three kinds of persimmon treated after harvest decreased and increased respectively, and the SOD activities of the vacuum group were the lowest，the APX activities under vacuum and vacuum+1-MCP conditions were higher than that in aerobic group. At vacuum and vacuum+1-MCP conditions，the increase of CAT activity of persimmon was inhibited, and the accumulation of H2O2 was reduced, with lower ability to inhibit hydroxyl radical and ascorbic acid content. The reducing sugar content of persimmon in vacuum and vacuum+1-MCP groups was lower than that in aerobic group, and the increase in vacuum+1-MCP group was the slowest, from 14.10% at the beginning of storage to 14.87% on 12 d. The pectin content of persimmon in vacuum+1-MCP group decreased slowly. Correlation analysis showed that the correlations between browning and various indexes under different postharharvest treatments were different. The persimmon BI was always negatively correlated with pectin, meanwhile positively correlated with reducing sugar. It can be concluded that vacuum and vacuum+1-MCP conditions are more conducive to inhibiting the browning of persimmon after deastringent

Enhanced anti−tumor efficacy of “IL−15 and CCL19” −secreting CAR−T cells in human glioblastoma orthotopic xenograft model

Despite the remarkable success of CAR-T cell therapy in hematologic malignancies, its progress in solid tumors has been slow. Overcoming challenges such as the recruitment and infiltration of CAR-T cells into the tumor site and the survival issues in the harsh tumor microenvironment are crucial for successful application in solid tumors. In this study, CAR-T cells were engineered to secrete both IL-15 and CCL19, and their efficacy was evaluated in a human glioblastoma orthotopic xenograft model. The results reveal that 15 × 19 CAR-T cells exhibit superior proliferation, chemotaxis, and phenotypic characteristics compared to conventional CAR-T cells in vitro. In vivo, 15 × 19 CAR-T cells exhibit superior control over tumors compared to conventional counterparts. Mechanistically, the improved efficacy can be attributed, in part, to IL-15 and CCL19 enhancing T-cell infiltration at the tumor site and fortifying resistance to exhaustion within the tumor microenvironment. In conclusion, the incorporation of IL-15 and CCL19 into CAR-T cells emerges as a promising strategy to elevate the anti-tumor efficacy of CAR-T cell therapy, positioning 15 × 19 CAR-T cells as a potential breakthrough for enhancing the application of CAR-T therapy in solid tumors

Long-Duration Vocational Education’s Effects on Individuals’ Vocational Identity, Self-Efficacy, and Job Satisfaction

This paper focuses on students receiving vertically integrated vocational education and evaluates the impacts of long-duration vocational education (LDVE) on individuals’ vocational identity, self-efficacy, and job satisfaction. Based on 1878 survey data, a logit model was used to analyze the influencing factors of students’ participation in the vertically integrated vocational education (VIVE) program, and the propensity score matching (PSM) method was used to measure the effects. Moreover, the comparison was made in different VIVE models to find the heterogeneity of effects. The results showed that family background and major significantly negatively affected the odds of an individual receiving VIVE. Merely extending the duration of vocational education does not significantly improve vocational identity, self-efficacy, and job satisfaction. Furthermore, the results of the between-group difference analysis indicated that neither the secondary-to-higher VIVE model nor the higher-to-undergraduate VIVE model had a significant impact on individuals’ vocational identity, self-efficacy, and job satisfaction. This research finding expands on the research achievements regarding the effects of LDVE. It can provide data references for the government and relevant institutions to pay attention to the quality and potential influence factors of LDVE

A Comprehensive Survey of RGB-Based and Skeleton-Based Human Action Recognition

With the advancement of computer vision, human action recognition (HAR) has shown its broad research worth and application prospects in a wide range of fields such as intelligent security, automatic driving and human-machine interaction. Based on the type of data captured by cameras and sensors, e.g., RGB, depth, skeleton, and infrared data, HAR methods can be classified into RGB-based and skeleton-based. RGB data is easy and inexpensive to obtain, but RGB-based methods need to cope with a large amount of irrelevant background information and are easily affected by factors such as lighting and shooting angle. The skeleton-based methods eliminate the impact of background variables and require little computational work due to their skeleton-focused features, but they lack the context data necessary for HAR. This paper gives a thorough survey of these two approaches, covering deep learning methods, handcrafted feature extraction methods, common datasets, challenges, and future research directions. The skeleton-based action recognition methods Section specifically presents the most well-liked 2D and 3D pose estimation algorithms. This survey aims to give researchers new to the area or engaged in a long-term study a selection of datasets and algorithms, as well as an overview of the present issues and expected future directions in the field

Tris[hexaamminecobalt(III)] bis[trioxalatocobaltate(II)] chloride dodecahydrate

The title compound, [CoIII(NH3)6]3[CoII(C2O4)3]2Cl·12H2O, was synthesized under hydrothermal conditions. The asymmetric unit comprises two [Co(NH3)6]3+ cations, one located on a threefold axis and the other on a site of symmetry -3, a [Co(C2O4)3]4+ anion, located on a threefold axis, one sixth of a chloride anion [disordered over two sites, one threefold (site occupancy = 0.5) and the other -3 (site occupancy (0.25)] and two water molecules. Both CoIII centers are six-coordinated by NH3 molecules, forming [Co(NH3)6]3+ octahedra, with Co—N distances in the range 1.958 (2)–1.977 (3) Å. The title structure gives the first example of the [Co(C2O4)3]4− anion, with the distorted octahedral environment of CoII center formed by six O atoms from three oxalate residues. The Co—O bond lengths are 2.0817 (18) to 2.0979 (18) Å. Multiple N—H...O, N—H...Cl and O—H...O hydrogen bonds link the cations, anions and water molecules into a three-dimensional network