Caveolae: biochemical analysis

aveolae appear in a multitude of processes encompassing growth regulation andtrafficking. We demonstrate the abundant presence of ESA/reggie-1/flotillin-2,ATP synthase β subunit and annexin V in endothelial caveolae byimmunopurification of caveolae from vascular endothelial membrane. Fiveproteins are abundant in a caveolin-1 protein complex, analyzed by sucrosegradient velocity sedimentation following octyl-β-D-glucopyranosideextraction. Caveolin-1 α interacts with caveolin-1 β, caveolin-2, actin,the microsomal form of NADH cytochrome B5 reductase and ESA/reggie-1/flotillin-2as shown by co-immunoprecipitation. We propose the concept that ATP biosynthesisin caveolae regulates mechanosignaling and is induced by membrane depolarizationand a proton gradient. Pressure stimuli and metabolic changes may trigger generegulation in endothelial cells, involving a nuclear conformer of caveolin-1,shown here with an epitope-specific caveolin-1 antibody, and immediate responseof ion channel activity, regulated by ESA/reggie-1/flotillin-

POLICE USE OF TECHNOLOGY TO FIGHT AGAINST CRIME

Traditionally, law enforcement agencies have had an unfriendly relationship with technology. However, there is no way one can ignore and/or resist the adoption of new technologies any longer since recent developments in information technology have changed the attitudes and perceptions of police forces as well as criminals. The technological advances over the years have provided law enforcement agencies new perspectives and considerations beyond the traditional methods and opportunities to utilize a wide range of innovations in different contexts. The recent innovations and implementations which increase the efficiency and effectiveness of policing including network analysis, GIS, crime mapping, biometrics, fingerprints, DNA research, facial recognition, speech recognition, social media policing, shotspotter detection system, and CCTV are detailed in this study

POLICE USE OF TECHNOLOGY TO FIGHT AGAINST CRIME

Traditionally, law enforcement agencies have had an unfriendly relationship with technology. However, there is no way one can ignore and/or resist the adoption of new technologies any longer since recent developments in information technology have changed the attitudes and perceptions of police forces as well as criminals. The technological advances over the years have provided law enforcement agencies new perspectives and considerations beyond the traditional methods and opportunities to utilize a wide range of innovations in different contexts. The recent innovations and implementations which increase the efficiency and effectiveness of policing including network analysis, GIS, crime mapping, biometrics, fingerprints, DNA research, facial recognition, speech recognition, social media policing, shotspotter detection system, and CCTV are detailed in this study

Coronary-Cameral Fistula with Angina Pectoris

Coronary-cameral fistula (CCF) is an anomalous connection between a coronary artery and cardiac chamber. Most of CCFs are discovered incidentally during angiographic evaluation for coronary vascular disorder. We report a case of CCF with angina pectoris. Selective coronary arteriography revealed diffuse CCF involving the left anterior descending artery (LAD) emptying into left ventricle (LV) and showed significant two-vessel coronary artery stenosis

Guillain-Barré Syndrome after Thrombolysis with Streptokinase

Guillain-Barre syndrome (GBS) is an eponym for a heterogeneous group of immune-mediated peripheral neuropathies. We describe a case of GBS in a patient who recieved intravenous streptokinase therapy for acute anterior myocardial infarction. Clinical symptoms are thought to result from streptokinase-antibody complex mediated damage to the local blood-nerve barrier. Patient was treated with 5-days course of intravenous gammaglobulin and his outcome was good