Chemotactic properties of Escherichia coli mutants having abnormal Ca2+ content

The calA, calC, and calD mutants of Escherichia coli are known to be sensitive to Ca2+ (R. N. Brey and B. P. Rosen, J. Bacteriol. 139:824-834, 1979). In the absence of any added stimuli for chemotaxis, both the calC and the calD mutants swam with a tumbly bias. Both the calC and the calD mutants were defective in chemotaxis as measured by computer analysis, use of swarm plates, and capillary assays. The calA mutant was only slightly defective in motility and only slightly impaired in chemotaxis. Chemotactically wild-type cells had an intra-cellular free-Ca2+ level of about 105 nM. The intracellular free-Ca2+ levels of the mutants, as determined by use of the fluorescent Ca2+ indicator dye fura-2 or fluo-3, were about 90, about 1,130, and about 410 nM for calA, calC, and calD, respectively. Lowering the intracellular free-Ca2+ levels in wild-type cells and in the tumbly cal mutants by use of Ca2+ chelators promoted running (smooth swimming). Overexpression of CheZ (which causes dephosphorylation of CheY-phosphate) in the wild type and in the tumbly cal mutants decreased the level of tumbliness (which is caused by CheY-phosphate). The calA mutant was 4- to 10-fold more resistant than the wild type to the inhibitory effect of omega-conotoxin on chemotaxis. omega-Conotoxin had no effect on Ca2+ extrusion by wild-type E. coli; that result suggests that omega-conotoxin affects Ca2+ transport at the point of entry instead of exit

Indicadors de pressió ambiental a les platges de Menorca : prova pilot (IPAPM'pp)

L'augment del turisme experimentat des de principis dels anys 70 a l'illa de Menorca (Illes Balears, Espanya) ha provocat un impacte sobre les seves platges, el seu principal atractiu turístic. Molts d'aquests impactes s'incrementen durant la temporada alta, compresa entre els mesos de maig a octubre. L'objectiu principal d'aquest projecte és l'elaboració d'un sistema d'indicadors de pressió ambiental útils per a l'anàlisi de les platges. La zona objecte d'estudi de la prova-pilot, correspon a les platges del sud-oest de Menorca, aquestes cales són les que actualment es troben sotmeses a una major pressió. Es tracta de catorze platges tipificades en tres categories (A, B, C) segons les característiques del tipus d'espai on es troben situades. Són les platges de: Cala Degollador, Cala Blanca, Cala'n Bosch, Son Xoriguer, Son Saura-Es Banyul, Son Saura-Bellavista, Es Talaier, Cala Turqueta, Cala Macarelleta, Cala Macarella, Cala Galdana, Cala Mitjana, Cala Trebalúger i Cala Escorxada. Partint d'un treball bibliogràfic, s'ha realitzat una selecció de mig centenar d'indicadors potencials dels quals catorze han format part dels IPAPM'pp, mitjançant l'elaboració d'una anàlisi multicriteri. Per a cada un dels IPAPM'pp s'ha desenvolupat una metodologia amb les corresponents fitxes individuals descriptives per al seu seguiment temporal. En aquest primer estudi, l'indicador que presenta un nombre de valors no acceptables en una major proporció de platges, és l'indicador 3. Índex de valoració de les mesures de conservació del sistema natural, seguit de l'indicador 6. Superfície subsistema sorra per usuari. El 2006, les platges de tipologia A, presenten un major percentatge positiu de les variables dels indicadors. Les platges de tipologia B i C presenten un percentatge menor d'acceptabilitat dels valors dels indicadors.El aumento del turismo experimentado desde principios de los años 1970 en la isla de Menorca (Islas Baleares, España) ha provocado un impacto sobre sus playas, su principal atractivo turístico. Muchos de los impactos se incrementan durante la temporada alta, comprendida entre los meses de mayo a octubre. El objetivo principal de este proyecto es la elaboración de un sistema de indicadores de presión ambiental útiles para el análisis de las playas. La zona objecto de estudio de la prueba-piloto, corresponde a las playas del sur-oeste de Menorca, estas calas són las que actualmente se encuentran sometidas a una mayor presión. Se trata de catorce playas tipificadas en tres categorías (A, B, C) según las características del tipo de espacio donde se encuentran situadas. Son las playas de: Cala Degollador, Cala Blanca, Cala'n Bosch, Son Xoriguer, Son Saura-Es Banyul, Son Saura-Bellavista, Es Talaier, Cala Turqueta, Cala Macarelleta, Cala Macarella, Cala Galdana, Cala Mitjana, Cala Trebalúger i Cala Escorxada. Partiendo de un trabajo bibliográfico, se ha realizado una selección de medio centenar de indicadores potenciales de los cuales catorce han formado parte de los IPAPM'pp, mediante la elaboración de un análisis multicriterio. Para cada uno de los IPAPM'pp se ha desarrollado una metodología con las correspondientes fichas individuales descriptivas para su seguimiento temporal. En este primer estudio, el indicador que presenta un mayor número de valores no aceptables en una mayor proporción de playas, es el indicador 3. Índice de valoración de las medidas de conservación del sistema natural, seguido del indicador 6. Superficie subsistema arena por usuario.The tourism in Menorca has been increased since 1970s (Balearic Islands, Spain), this fact has caused an impact on its beaches, which are the main tourist attraction. Most of the impacts have been increased during the high season, between May and October. The principal objective of this project is the development of an environmental pressure indicators system, which could be useful for beaches analysis. The area that has been studied belongs to the south-west area of Menorca, which is the most pressed area. It is an amount of fourteen beaches classified in three categories (A, B, C) depending on their local situation. These beaches are: Cala Degollador, Cala Blanca, Cala'n Bosch, Son Xoriguer, Son Saura-Es Banyul, Son Saura-Bellavista, Es Talaier, Cala Turqueta, Cala Macarelleta, Cala Macarella, Cala Galdana, Cala Mitjana, Cala Trebalúger and Cala Escorxada. The bibliography research job, has been realised a selection of half hundred of potential indicators, that only fourteen of them has composed the IPAPM'pp (Environmental Pressure Indicators of Menorca Beaches. Pilot Proof) by a multicriteria analysis. For each of the IPAPM'pp has been developed a methodology with an individual describing form in order to its temporal monitoring. In this first study, the indicator whose values are the worst is indicator 3. Quality factor of the natural system conservation measures. Indicator 6. Area of sand subsystem per user is the second indicator which has the worse values.Nota: Aquest document conté originàriament altre material i/o programari només consultable a la Biblioteca de Ciència i Tecnologia

Book review: America’s blind spot: Chávez, oil and US security

Latin America holds some of the world’s biggest oil reserves, but unstable political events in the region are hindering its potential, especially in Venezuela. Global U.S. security would benefit from a revamping of outdated policies towards Latin America, argue Andrés Cala and Michael Economides. This is a blind spot in American politics, one that threatens U.S. geopolitical and economic interests. In this book, the authors aim to offer a thorough analysis of key geopolitical and economic threats to the U.S., highlighting the need for a new Latin American policy doctrine based on military and strategic priorities. Reviewed by Luis Boscán. America’s Blind Spot: Chávez, Oil and US Security. Andrés Cala and Michael Economides. Continuum Books. 201

Relational Spacetime Ontology

In the aftermath of the rediscovery of Einstein’s hole argument by Earman and Norton (1987), we hear that the ontological relational/substantival debate over the status of spacetime seems to have reached stable grounds. Despite Einstein’s early intention to cast GR’s spacetime as a relational entity à la Leibniz-Mach, most philosophers of science feel comfortable with the now standard sophisticated substantivalist (SS) account of spacetime. Furthermore, most philosophers share the impression that although relational accounts of certain highly restricted models of GR are viable, at a deep down level, they require substantival spacetime structures. SS claims that although manifold spacetime points do not enjoy the sort of robust existence provided by primitive identity, it is still natural to be realistic about the existence of spacetime as an independent entity in its own right. It is argued that since the bare manifold lacks the basic spacetime structures –such as geometry and inertia- one should count as an independent spacetime the couple manifold +metric (M, g). The metric tensor field of GR encodes inertial and metrical structure so, in a way, it plays the explanatory role that Newtonian absolute space played in classical dynamics. In a nutshell, according to the SS account of spacetime, one should view the metric field of GR as the modern version of a realistically constructed spacetime since it has the properties –or contains the structures- that Newtonian space had. I will try to dismantle the widespread impression that a relational account of full GR is implausible. To do so, I will start by highlighting that when turning back to the original Leibniz-Newton dispute one sees that substantivalism turns out prima facie triumphant since Newton was able to successfully formulate dynamics. However, to give relationalism a fair chance, one can also put forward the following hypothetical questions: What if Leibniz –or some leibnizian- had had a good relational theory? What role would geometry play in this type of theory? Would it be natural to view geometry and inertia as intrinsic properties of substantival space –if not spacetime? Would it still seem natural to interpret the metric field of GR along substantival lines regardless of the fact that it also encodes important material properties such as energy-momentum? After bringing these questions out into the light I will cast some important doubts on the substantival (SS) interpretation of the metric field. Perhaps the metric turns out to be viewed as a relational matter field. Finally, to strengthen the relational account of spacetime I expect to remove the possible remaining interpretative tension by briefly discussing the relevance of two important facts: i) Dynamical variables are usually linked to material objects in physical theories. The metric field of GR is a dynamical object so, I claim, it should be viewed as a matter field. ii) Barbour and Bertotti (BB2, 1982) have provided and alternative formulation of classical dynamics. They provide a “genuinely relational interpretation of dynamics” (Pooley & Brown 2001). Geometry and inertia become –contra SS- relational structures in BB2

Activation of electroneutral K flux in Amphiuma red blood cells by N-ethylmaleimide. Distinction between K/H exchange and KCl cotransport.

Exposure of Amphiuma red blood cells to millimolar concentrations of N-ethylmaleimide (NEM) resulted in net K loss. In order to determine whether net K loss was conductive or was by electroneutral K/H exchange or KCl cotransport, studies were performed evaluating K flux in terms of the thermodynamic forces to which K flux by the above pathways should couple. The direction and magnitude of the NEM-induced net K flux did not correspond with the direction and magnitude of the forces relevant to K conductance or electroneutral KCl cotransport. Both the magnitude and direction of the NEM-activated K flux responded to the driving force for K/H exchange. We therefore conclude that NEM-induced K loss, like that by osmotically swollen Amphiuma red blood cells, is by an electroneutral K/H exchanger. In addition to the above studies, we evaluated the kinetic behavior of the volume- and NEM-induced K/H exchange flux pathways in media where Cl was replaced by SCN, NO3, para-aminohippurate (PAH), or gluconate. The anion replacement studies did not permit a distinction between K/H exchange and KCl cotransport, since, depending upon the anion used as a Cl replacement, partial inhibition or stimulation of volume-activated K/H exchange fluxes was observed. In contrast, all anions used were stimulatory to the NEM-induced K loss. Since, on the basis of force-flow analysis, both volume-and NEM-induced K loss are K/H exchange, it was necessary to reevaluate assumptions (i.e., anions serve as substrates and therefore probe the translocation step) associated with the use of anion replacement as a means of flux route identification. When viewed together with the force-flow studies, the Cl replacement studies suggest that anion effects upon K/H exchange are indirect. The different anions appear to alter mechanisms that couple NEM exposure and cell swelling to the activation of K/H exchange, as opposed to exerting direct effects upon K and H translocation

Regularity of the stress-energy tensor for extremal Reissner-Nordstrom black holes

We calculate the expectation values of the stress-energy tensor for both a massless minimally-coupled and dilaton-coupled 2D field propagating on an extremal Reissner-Nordstrom black hole, showing its regularity on the horizon in contrast with previous claims in the literature.Comment: 10 pages, 1 figure; Talk given at QG05, Cala Gonone (Italy), September 200