Genome Sequence of the Native Apiculate Wine Yeast Hanseniaspora vineae T02/19AF

The use of novel yeast strains for winemaking improves quality and provides variety including subtle characteristic differences in fine wines. Here we report the first genome of a yeast strain native to Uruguay, Hanseniaspora vineae T02/19AF, which has been shown to positively contribute to aroma and wine quality.Fil: Giorello, Facundo M.. Universidad de la República; UruguayFil: Berná, Luisa. Instituto Pasteur de Montevideo; UruguayFil: Greif, Gonzalo. Instituto Pasteur de Montevideo; UruguayFil: Camesasca, Laura. Inst. de Investigaciones Biológicas Clemente Estable; UruguayFil: Salzman, Valentina. Instituto Pasteur de Montevideo; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Medina, Karina. Universidad de la Republica. Facultad de Química; UruguayFil: Robello, Carlos. Instituto Pasteur de Montevideo; UruguayFil: Gaggero, Carina. Inst. de Investigaciones Biológicas Clemente Estable; UruguayFil: Aguilar, Pablo S.. Instituto Pasteur de Montevideo; UruguayFil: Carrau, Francisco. Sección Enología; Urugua

Global mixing indices for single screw extrusion

The present work presents numerical simulations of dispersive and distributive mixing of a two phase system being processed in a single screw extruder under various conditions. Models were developed to quantify the degree of mixing of liquid-liquid and solids-liquid systems, for a given set of material properties, operating conditions and extruder geometry

Numerical and experimental study of agglomerate dispersion in polymer extrusion

A model for agglomerate dispersion in screw extruders was developed and superimposed on the flow patterns as simulated using the FIDAP software. A particle tracking algorithm with an adaptive time step was used to follow the agglomerates trajectory. Along this flow path, the breakup probability was estimated using a Monte Carlo method and in conjunction with the local fragmentation number. Particle size distributions and Shannon entropy were computed along the screw channel. The results show good qualitative agreement between model predictions and experimental data

Implementation of the Damages Directive in England & Wales

The Dossier discusses the questions arising from the need to implement the EU Damages Directive 2014/104/EU in several European Member States. My contribution focuses on the need for implementation in England & Wales

Scale-down perfusion bioreactor optimization for the production of therapeutic oligonucleotides

LAUREA MAGISTRALEGli oligonucleotidi terapeutici a RNA stanno suscitando un crescente interesse nel panorama farmaceutico odierno, soprattutto grazie alla possibilità di estendere la gamma di bersagli farmacologici dalle proteine convenzionali a specifiche sequenze di RNA e al genoma stesso. Uno dei metodi più promettenti per la produzione di queste molecole è rappresentato dalla sintesi in-vivo, che presenta diversi vantaggi rispetto alla sintesi chimica o in-vitro. Il batterio marino viola Rhodovulum Sulfidophilum si è rivelato essere un ottimo mezzo per la produzione ricombinante di molecole di RNA artificiali grazie alla capacità di secernere gli acidi nucleici prodotti nell'ambiente extracellulare e all'assenza di emissioni rilevabili di RNase nell'ambiente di cultura. In questo modo, la produzione di acidi nucleici per mezzo di fermentazione di R. Sulfidophilum in bioreattori a perfusione combina le caratteristiche positive della sintesi in-vivo con i vantaggi legati a un processo produttivo in continuo. In questo lavoro è stato adottato un approccio di ottimizzazione in scala ridotta (scale-down experiment) per lo screening di differenti condizioni operative. Sono stati eseguiti due esperimenti del tipo VCDmax in microscala (più uno precedentemente condotto dal nostro gruppo di ricerca) con lo scopo di testare il comportamento delle culture sottoposte a diversi tassi di perfusione quando esposte a diverse condizioni di aerazione. L'affidabilità di questo metodo è stata convalidata confrontando i risultati degli esperimenti in spin-tube con i dati raccolti dal bioreattore a perfusione su scala di laboratorio.Therapeutics RNA oligonucleotides are assuming increasing interest in the present day pharmaceutical landscape thanks to the possibility of extending the range of druggable targets from conventional proteins to RNAs and to the genome itself. One of the most promising method for the production of these molecules is represented by the in-vivo synthesis, that presents several advantage respect to chemical or in-vitro synthesis. Rhodovulum Sulfidophilum purple marine bacterium proved to be a suitable host for the recombinant production of artificial RNA molecules thanks to the capacity of extracellular nucleic acid secretion and to the absence of observable RNases emissions in the culture environment. In this way, nucleic acids production by means of R. Sulfidophilum fermentation in perfusion bioreactors combines the characteristics of in-vivo synthesis with the advantages related to a continuous productive process. In this work a scale-down optimization approach has been adopted for screening multiple operating conditions. Two micro-scale VCDmax experiments (plus one previously conducted by our research group) have been performed for testing the culture's behavior at various perfusion rates when exposed to different aeration conditions. The reliability of this method was cross-validated by comparing the results of spin-tube experiments with the data collected from benchtop-scale perfusion bioreactor. Further measurements on nucleic acid concentration in the culture environment have been performed to relate the cells productivity with the operating conditions. In future work, further benchtop-scale experiments are required for the assessment of performance parameters for high-densities cultivations and to provide samples for the quantitative and qualitative analyses of oligonucleotides productivity

Determinants of the duration of European appellate court proceedings in cartel cases

The duration of appellate court proceedings is an important determinant of the efficiency of a court system. We use data of 234 firm groups that participated in 63 cartels convicted by the European Commission between 2000 and 2012 to investigate the determinants of the duration of the subsequent one- or two-stage appeals process. We find that while the speed of the firststage appellate court decision depends on the court’s appeals-related workload, the complexity of the case, the degree of cooperation by the firms involved and the clarity of the applied rules and regulations, the second-stage appellate court proceedings appear to be largely unaffected by those drivers. We take our empirical results to derive conclusions for both firms that plan to file an appeal as well as public policy makers

Entropic Characterization of Mixing in Microchannels

A methodology for rigorous mixing assessment in microchannels is presented. The analysis is based on numerical simulations of flow in different geometries coupled with mixing assessment using entropic measures. The results show enhanced mixing efficiency for the staggered herringbone micromixer by comparison with a mixer with straight diagonal ridges and a lack of mixing in a non-patterned channel. These results are in agreement with published experimental data

Espansi poliuretanici biodegradabili: applicazioni per ingegneria dei tessuti

LAUREA SPECIALISTIC

Quantifying Fluid Mixing with The Shannon Entropy

We introduce a methodology to quantify the quality of mixing in various systems, including polymeric ones, by adapting the Shannon information entropy. For illustrative purposes we use particle advection of two species in a two-dimensional cavity flow. We compute the entropy by using the probability of finding a suitable chosen group/complex of particles of a given species, at a given location. By choosing the size of the group to be in direct proportion to the overall concentration of the components in the mixture we ensure that the entropic measure is maximized for the case of perfect mixing, that is, when at each location the component concentration is equal to the corresponding overall component concentrations. The scale of observation role in evaluating mixing is analyzed using the entropic methodology. We also illustrate the effect of initial conditions on mixing in a laminar system, typical in operations involving polymers

The Effect of Stocking Rate and Lamb Grazing System on Sward Performance of \u3cem\u3eTrifolium Repens\u3c/em\u3e and \u3cem\u3eLotus Corniculatus\u3c/em\u3e in Uruguay

Lambs have a great potential to diversify and stimulate meat and wool production and economical returns within the industry. The main objective of this study was to evaluate different feeding and management alternatives for the production of high quality wool and meat as well as their effects on sward characteristics in the Basaltic region of Uruguay