Canadian English-Language Children's Songs in Toronto Schools: A Comparison of 1959-64 and 1988 Collections

Virginia Caputo examine la transmission orale des chansons enfantines. Par une comparaison des chansons enregistrées pendant la période 1959-64 et un corpus enregistré aux mêmes écoles en 1988, elle analyse la stabilité et l’évolution caractérisant cette tradition musicale à travers le temps. Elle détermine que ces chansons reflètent les attitudes des enfants envers la classe sociale, le genre, le pouvoir, et l’ethnicité

Comparação entre dois métodos de determinação de cálcio ionizável em amostras de recém-nascidos

Changes in ionized calcium (iCa) concentration seem to occur in newborns when the calcium supply from the mother is disconnected at birth, and a related hypocalcemia is produced. Thus, measurement of iCa is of particular importance in newborns. The aim of this study was to compare two methods of iCa measurement. We collected 50 samples from newborns in which we obtained values of iCa using both the direct and the indirect measurement. There is a significant correlation between iCa values obtained from Omni C and those obtained from the calculated method.As alterações na concentração de cálcio ionizado (iCa) ocorrem nos recém-nascidos quando a fonte de cálcio proveniente da mãe é removida no momento do nascimento, resultando em hipocalcemia. Portanto, a verificação de iCa é de particular importância no recém-nascido. O objetivo deste estudo foi comparar dois métodos de determinação de iCa utilizados em laboratórios. Foram utilizadas 50 amostras de recém-nascidos que obtiveram valores de iCa pela medida direta e também pelo método indireto. Existe uma correlação significativa entre os valores de iCa obtidos utilizando Omni C e aqueles com o método calculado.Hospital Estadual Mário CovasUniversidade de São Paulo Instituto de QuímicaUniversidade Federal de São Paulo (UNIFESP)USP Faculdade de Ciências FarmacêuticasUNIFESPUSPUSP Faculdade de MedicinaUniversidade de Santo AmaroInstituto de Pesquisa e Educação em Saúde de São PauloFaculdade de Medicina do ABCUNIFESP FMABCUNIFESP, FMABCSciEL

Occurrence of Fusarium species in asymptomatic barley plants grown in field conditions under differential phosphate regimes

Occurrence of genus Fusarium as endophytes in roots of healthy barley plants cultivated in fields under two levels of phosphorous (P) was examined. F. avenaceum, F. graminearum, F. konzum, F. oxysporum, F. sacchari, F. scirpi, F. solani were isolated from asymptomatic surface sterilized root tissues. F. konzum and F. solani were only present in the roots of fertilized plants, whereas F. sacchari was present in unfertilized plants. F. scirpi was isolated in highest frequency and relative density, and was higher under P deficiency suggesting that P availability would determinate the occurrence of this species, suggesting the possibility of their association with stressed plants. This is the first report on the occurrence of Fusarium spp. as endophytes in healthy barley plants. However, it remains to be determined whether Fusarium spp. isolated are nonpathogenic forms and beneficial to barley plants or latent pathogens that could produce disease symptoms in other conditions.Fil: Echeverria, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Moreno, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentin

Influence of FFF Process Conditions on the Thermal, Mechanical, and Rheological Properties of Poly(hydroxybutyrate-co-hydroxy Hexanoate)

Polyhydroxyalkanoates are natural polyesters synthesized by microorganisms and bacteria. Due to their properties, they have been proposed as substitutes for petroleum derivatives. This work studies how the printing conditions employed in fuse filament fabrication (FFF) affect the properties of poly(hydroxybutyrate-co-hydroxy hexanoate) or PHBH. Firstly, rheological results predicted the printability of PHBH, which was successfully realized. Unlike what usually happens in FFF manufacturing or several semi-crystalline polymers, it was observed that the crystallization of PHBH occurs isothermally after deposition on the bed and not during the non-isothermal cooling stage, according to calorimetric measurements. A computational simulation of the temperature profile during the printing process was conducted to confirm this behavior, and the results support this hypothesis. Through the analysis of mechanical properties, it was shown that the nozzle and bed temperature increase improved the mechanical properties, reducing the void formation and improving interlayer adhesion, as shown by SEM. Intermediate printing velocities produced the best mechanical properties.This work has received funding from the Basque Government through grant IT1503-22, the Gipuzkoa Provincial Council (Red I+D+i 2022-CIEN-000022-01), and the project National Centre of Competence in Polymer Materials and Technologies for the 21st Century no. TN02000051 (TA CR, Czech Republic)

Measurements of the pp → ZZ production cross section and the Z → 4ℓ branching fraction, and constraints on anomalous triple gauge couplings at √s = 13 TeV

Four-lepton production in proton-proton collisions, pp -> (Z/gamma*)(Z/gamma*) -> 4l, where l = e or mu, is studied at a center-of-mass energy of 13 TeV with the CMS detector at the LHC. The data sample corresponds to an integrated luminosity of 35.9 fb(-1). The ZZ production cross section, sigma(pp -> ZZ) = 17.2 +/- 0.5 (stat) +/- 0.7 (syst) +/- 0.4 (theo) +/- 0.4 (lumi) pb, measured using events with two opposite-sign, same-flavor lepton pairs produced in the mass region 60 4l) = 4.83(-0.22)(+0.23) (stat)(-0.29)(+0.32) (syst) +/- 0.08 (theo) +/- 0.12(lumi) x 10(-6) for events with a four-lepton invariant mass in the range 80 4GeV for all opposite-sign, same-flavor lepton pairs. The results agree with standard model predictions. The invariant mass distribution of the four-lepton system is used to set limits on anomalous ZZZ and ZZ. couplings at 95% confidence level: -0.0012 < f(4)(Z) < 0.0010, -0.0010 < f(5)(Z) < 0.0013, -0.0012 < f(4)(gamma) < 0.0013, -0.0012 < f(5)(gamma) < 0.0013

Search for heavy resonances decaying to two Higgs bosons in final states containing four b quarks

A search is presented for narrow heavy resonances X decaying into pairs of Higgs bosons (H) in proton-proton collisions collected by the CMS experiment at the LHC at root s = 8 TeV. The data correspond to an integrated luminosity of 19.7 fb(-1). The search considers HH resonances with masses between 1 and 3 TeV, having final states of two b quark pairs. Each Higgs boson is produced with large momentum, and the hadronization products of the pair of b quarks can usually be reconstructed as single large jets. The background from multijet and t (t) over bar events is significantly reduced by applying requirements related to the flavor of the jet, its mass, and its substructure. The signal would be identified as a peak on top of the dijet invariant mass spectrum of the remaining background events. No evidence is observed for such a signal. Upper limits obtained at 95 confidence level for the product of the production cross section and branching fraction sigma(gg -> X) B(X -> HH -> b (b) over barb (b) over bar) range from 10 to 1.5 fb for the mass of X from 1.15 to 2.0 TeV, significantly extending previous searches. For a warped extra dimension theory with amass scale Lambda(R) = 1 TeV, the data exclude radion scalar masses between 1.15 and 1.55 TeV

A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors

A cohort of 47 patients is screened for pancreatic cancer precursors with a portable 96-well bioelectronic sensing-array for single-molecule assay in cysts fluid and blood plasma, deployable at point-of-care (POC). Pancreatic cancer precursors are mucinous cysts diagnosed with a sensitivity of at most 80% by state-of-the-art cytopathological molecular analyses (e.g., KRASmut DNA). Adding the simultaneous assay of proteins related to malignant transformation (e.g., MUC1 and CD55) is deemed essential to enhance diagnostic accuracy. The bioelectronic array proposed here, based on single-molecule-with-a-large-transistor (SiMoT) technology, can assay both nucleic acids and proteins at the single-molecule limit-of-identification (LOI) (1% of false-positives and false-negatives). It comprises an enzyme-linked immunosorbent assay (ELISA)-like 8 × 12-array organic-electronics disposable cartridge with an electrolyte-gated organic transistor sensor array, and a reusable reader, integrating a custom Si-IC chip, operating via software installed on a USB-connected smart device. The cartridge is complemented by a 3D-printed sensing gate cover plate. KRASmut, MUC1, and CD55 biomarkers either in plasma or cysts-fluid from 5 to 6 patients at a time, are multiplexed at single-molecule LOI in 1.5 h. The pancreatic cancer precursors are classified via a machine-learning analysis resulting in at least 96% diagnostic-sensitivity and 100% diagnostic-specificity. This preliminary study opens the way to POC liquid-biopsy-based early diagnosis of pancreatic-cancer precursors in plasma.</p

A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors

A cohort of 47 patients is screened for pancreatic cancer precursors with a portable 96-well bioelectronic sensing-array for single-molecule assay in cysts fluid and blood plasma, deployable at point-of-care (POC). Pancreatic cancer precursors are mucinous cysts diagnosed with a sensitivity of at most 80% by state-of-the-art cytopathological molecular analyses (e.g., KRASmut DNA). Adding the simultaneous assay of proteins related to malignant transformation (e.g., MUC1 and CD55) is deemed essential to enhance diagnostic accuracy. The bioelectronic array proposed here, based on single-molecule-with-a-large-transistor (SiMoT) technology, can assay both nucleic acids and proteins at the single-molecule limit-of-identification (LOI) (1% of false-positives and false-negatives). It comprises an enzyme-linked immunosorbent assay (ELISA)-like 8 × 12-array organic-electronics disposable cartridge with an electrolyte-gated organic transistor sensor array, and a reusable reader, integrating a custom Si-IC chip, operating via software installed on a USB-connected smart device. The cartridge is complemented by a 3D-printed sensing gate cover plate. KRASmut, MUC1, and CD55 biomarkers either in plasma or cysts-fluid from 5 to 6 patients at a time, are multiplexed at single-molecule LOI in 1.5 h. The pancreatic cancer precursors are classified via a machine-learning analysis resulting in at least 96% diagnostic-sensitivity and 100% diagnostic-specificity. This preliminary study opens the way to POC liquid-biopsy-based early diagnosis of pancreatic-cancer precursors in plasma.</p