Effectiveness of a novel extraction method for semen: comparison using liquid samples and dried stains

Forensic analysis of deoxyribonucleic acid (DNA) collected from sexual assault evidence is a multi-step process that requires a great amount of time and resources. A large percentage of samples are mixtures containing DNA from a major female contributor and at least one minor male contributor. The amount of male DNA present is often much less than that of the female, making it difficult to achieve a full short-tandem repeat (STR) profile for identification purposes. The current method employed by many forensic laboratories to separate sperm DNA from non-sperm DNA is the differential extraction. Although a robust and reliable method when applied to liquid samples, the procedure has failed to evolve significantly since first developed.1,2 Between the time it has been collected and tested, sexual assault evidence becomes dried and aged, contributing to the potential loss and degradation of already low amounts of DNA and increasing the likelihood of an incomplete profile.2 This study tests the effectiveness of a combination of enzymes to release DNA from sperm using a variety of substrates. Although this method extracted greater amounts of male DNA than the traditional Qiagen® extraction, further research is necessary to determine if the application of this new method can improve or eventually replace the current procedures.2018-06-16T00:00:00

Description of eight new species of the traumatically inseminating plant bug genus Coridromius (Heteroptera: Miridae: Orthotylinae: Coridromini)

Eight new species of the plant bug genus Coridromius are described: C. basilanus sp. nov. from the Philippines, C. eremnos sp. nov. from Sabah, Malaysia, C. fomangsu sp. nov. and C. tafo sp. nov. from Ghana, C. norfolkensis sp. nov. from Norfolk Island, Australia, C. mulu sp. nov. from Sarawak, Malaysia, C. macchabeeus sp. nov. from Mauritius, and C. taravao sp. nov. from Tahiti, French Polynesia

Necrosis isquémica después de reducción cerrada en displasia del desarrollo de la cadera

Se presenta una revisión retrospectiva de 81 pacientes con 121 caderas con displasia del desarrollo tratados mediante reducción cerrada, miotomía de los aductores, tenotomía del psoas e inmovilización en una espica de yeso sin tracción previa a la reducción. Setenta y un pacientes del sexo femenino y 10 del masculino. El período de inmovilización fue en promedio de 3,6 meses. El seguimiento fue de 3 años mínimo y 20 años como máximo, con un promedio de 9,8 años. Los pacientes tratados fueron divididos en 2 grupos: grupo I, 93 caderas en pacientes de 7 a 18 meses de edad, y el grupo II, 28 caderas de pacientes de 19 meses a 3 años de edad. El patrón de lesión isquémica fue determinado de acuerdo con la clasificación de Bucholz y Ogden. Todas las radiografías fueron revisadas para establecer la presencia de deformidad de la cabeza femoral, displasia acetabular, subluxación, sobrecrecimiento del trocánter mayor y discrepancia en la longitud de las extremidades. En el grupo I se encontró afección en 40 caderas (43%) con cambios isquémicos moderados en 6 casos y leves en 9. De acuerdo con los criterios clínicos de Barret, 39 caderas tuvieron una evolución clínica excelente-buena y 1 caso regular. Los criterios radiológicos de Severin clasifican 37 caderas en los grupos I-II y 3 en el grupo III. En el grupo II, 23 caderas (82%) presentaron cambios isquémicos, leves en 5 casos, moderados en 4 y severos en 3. Con la clasificación de Barret, 19 caderas con resultados excelente-bueno y 4 con 1 resultado regular. Severin sitúa 21 caderas en los grupos l-II y 2 en el grupo IV. En ambos grupos se requirieron de procedimientos quirúrgicos adicionales. Los cambios isquémicos más severos estuvieron en relación con la edad del paciente.This is a 20 years retrospective review of 81 patients with 121 hips with developmental dysplasia of the hip treated by closed reduction, adductor myotomy and psoas tenotomy and immobilization in a spica cast, without prereduction traction. Divided in 2 groups, group 1: 93 hips from 7 to 18 months, and group II: 28 hips from 19 months to 3 years. The Bucholz-Ogden classification was used to determine the patterns of ischemic lesion and the final results were assessed according to Barret's clinical criteria and Severin's radiological rating. In group I (93 hips), 40 hips (43%) showed ischemic changes, mild (9 hips) to moderate (6 hips). The Barret clinical criteria showed 39 hips with excellent and good results and 1 hip fair. Severins radiological rating showed 37 hips in type l-II and 3 hips in type III. Group II (28 hips), 23 hips showed ischemic changes, mild in 5 hips, moderate 4 hips and severe in 3 hips. The Barret clinical criteria in the final assessment showed 19 hips with excellent-good results and 4 hips was fair. Severins radiological rating showed 21 hips in type I-II and 2 hips in type IV. Some of the patients needed additional surgical procedures. The ischemic changes were more severe as patients gets older

Financial History

The considerable renewal of interest in all aspects of financial history over recent years provided one motivation for this new venture. Yet, the foundations for our specialism, which draws from both History and the Social Sciences, especially economics, have been laid by many. Some would point to continuity in our interest from the publication in the 1930s of jubilee banking history volumes, such as those written for British institutions by Gregory, and by Crick and Wadsworth. Further scholarly momentum came from the studies in comparative banking history undertaken by researchers inspired and brought together by Rondo Cameron from the mid-1960s. Upon these footings and others, financial history has since ‘taken off' to address an increasingly wider range of issues. This considerable broadening of the specialism, ironically, almost coincided with the decline and then the eventual demise in 1987 of the Revue Internationale d'Histoire de la Banque, founded in 1968. This is not the place to draw up a balance sheet of that journal, which has played a decisive role. Its demise, however, left a gap. Financial History Review aims not only to take up its cause, but also to widen the scope of publishing in the field - from banking to financial history - in order to offer the fullest possible support for continuing researc

Hernia de disco lumbar en adolescentes

Se revisaron 19 pacientes en quienes se diagnosticó y trató de hernia de disco lumbar, cuyas edades fluctuaron entre 12 y 19 años, siendo 2 femeninos y 17 masculino. La sintomatología predominante fue dolor lumbar, alteraciones en la marcha y alteraciones neurológicas. El diagnóstico se realizó mediante mielografía simple, Mielo-TAC, TAC y RMI. Todos fueron tratados con laminectomía del segmento afectado y disquectomía. Los resultados obtenidos a la última visita fueron 15 pacientes asintomáticos, 2 pacientes con parestesias ocasionales y 2 con dolor lumbar esporádico, sin repercusión en sus actividades de la vida diaria.We analyzed 19 patients adolescents diagnosed and treated of herniated lumbar disc. The age range was 12 to 19 years old. Two were females and 17 were males. The most prevalent symptomatology was low back pain, abnormal neurological responses and frequent walking disturbances. The diagnosis tests were roentgenogram, myelography, myelo-CT scanning, CT scanning and MRI in all the patients. All the patients were surgically treated with diskectomy and interlaminal hemilaminectomy without fusion, by posterior approach, except in one patient with spondylolistesis. The postoperative results were excellent in 15 patients, 2 with occasional low back pain and 2 with sciatica. They were able to resume their normal activities by themselves