Mycochemical study of polysaccharides from the edible mushroom Cortinarius caperatus (Gypsy mushroom)

Among basidiomycete molecules, cell wall polysaccharides have been recognized as a major class of bioactive constituents. [1] They are safe molecules and they have a wide spectrum of biological activities, such as immunostimulatory and antioxidant, therefore they possess a prominent role in health benefits coming from mushroom consumption. These properties make mushroom polysaccharides potential candidates for nutraceutical applications and bioactive ingredients production. [2] Fractionation of the hot aqueous extract of Cortinarius caperatus led to isolation of two fractions characterized by spectroscopic analyses (1H-NMR, 13C-NMR, DEPT, 1H-1H COSY, DQCOSY, TOCSY, HSQC, HMBC and HMQC), mass spectrometry (EI-MS, ESI-MS), infrared spectroscopy (FT-IR), chemical reactions of hydrolysis and derivatization followed by GC and HPLC analyses. [3] This mycochemical study revealed a water-soluble fraction characterized as a \u3b2-(1\uf0e06)-D-glucan, whose presence inside C. caperatus has never, to the best of our knowledge, been reported before. Moreover, a water insoluble fraction purified has been characterized as a branched \uf061, (1\u21926) glucan which structure is assumed to be: [\u21926)-\u3b2-D-Glcp(1\u21926)]4-\u3b1-D-Glcp(1\u21924)-\u3b2-D-Glcp(1\u2192 6 \u2191 1 \u3b1-D-Glcp The antioxidant activity of the soluble polysaccharide fraction has been evaluated as radical-scavenging activity with the DPPH test, the \u3b2-(1\uf0e06)-D-glucan showed significative antioxidant activity

A New Bi-Functional Derivative of Polyethylene Glycol as Molecular Carrier for Eugenol and Ibuprofen

Eugenol (EU) and ibuprofene (IBU) were covalently bound to a bi-functionalized PEG, used as molecular carrier of drugs and the release kinetics of the two bioactive molecules was studied in vitro in buffer solution at pH 7.4, in simulated gastric fluid and in mouse plasma. The hydrolysis studies showed a specific cleavage dependent on the pH of the medium and by the presence of proteolytic enzymes in mouse plasma. Studies in vitro on the release of the parent drug from this double prodrug in various media, indicate that the adduct may be sufficiently stable to pass intact the gastrointestinal tract and release into the circulation EU and IBU. Many advantages may be achieved by the synthesis of the prodrug EU-PEG-IBU related to synergistic analgesic and anti-inflammatory effects, to the reduction of the adverse reactions and the improvement of the chemical-physical properties of the parent drugs

New Triterpenes from the Fungus Gloeophyllum odoratum

Two new triterpene acids (1, 2), together with a complex mixture of lecithins (3), were isolated from the fungus Gloeophyllum odoratum. Their structures were elucidated on the basis of extensive spectroscopic analyses. The extract of Gloeophyllum odoratum considerably inhibited thrombin (72% at a concentration of 120 g/mL); the less polar fraction of the methanolic extract from the mushroom exhibited interesting activity (47% at a concentration of 120 g/mL) against thrombin. Compounds 1 and 2 were responsible for this activity

CHOLANE AND LANOSTANE DERIVATIVES: ANTIMICROBIAL EVALUATION

Steroids are natural compounds with several important applications in many fields of research, such as medicinal chemistry, pharmacology, supramolecular chemistry and nanotechnology.In particular, bile acids such as lithocholic acid (LCA) and ursodeoxycholic acid (UDCA) have been considered quite useful as starting points for a rich and different set of medicinal chemistry activities. Besides, the discovery of bioactive ingredients from plants and fungi is always the main target in medicinal chemistry. The lanostane-type triterpenoid 3b-hydroxylanosta-8,24-diene-21-oic acid (Trametenolic acid, TMA) was the main bioactive component of Gloeophyllum odoratum, which was reported to possess widely bioactivities, including tumor cell anti-proliferation effects (for example, human HL-60 leukemia, human KB epidermoid carcinoma, murine L1210 leukemia cells, Caski, HT-3, T-24, etc.), inhibition of enzyme activity (human thrombin, bovine trypsin and so on).Nevertheless, trametenolic acid was scarcely investigated as antimicrobial agent. Structurally, bile acids (LCA and UDCA) and trametenolic acid are similar since they may be regarded as consisting of two components, a rigid steroid nucleus and an aliphatic side chain possessing a carboxyl group. On the basis of these considerations, six new compounds bearing a guanidine moiety in their side chain were synthesized using LCA, UDCA and TMA as starting materials. The parent bile acids, TMA and their resulting derivatives were evaluated for antimicrobial activity against S. aureus, B. subtilis and M. smegmatis. The derivative 3a-hydroxy-23-guanidino-5b-cholane showed the best activity, with MIC values of 12.5 \u3bcM against S. aureus, 5 \u3bcM against B. subtilis and 50 \u3bcM against M. smegmatis. The cytotoxic activity of bile acids, trametenolic acid and derivatives was also evaluated against HT-29 cell lin

Retention of arsenic and selenium compounds present in coal combustion and gasification flue gases using activated carbons

7 pages, 7 figures, 6 tables.-- Printed version published Aug 2007.The emission of potentially toxic compounds of arsenic and selenium present in flue gases from coal combustion and gasification processes has led to the need for gas cleaning systems capable of reducing their content. This work is focused on the capture of these elements in activated carbons which have proven to have good retention capacities for mercury compounds in gas phase. Two commercial activated carbons (Norit RBHG3 and Norit RB3) and a carbon prepared via activation of a pyrolysed coal (CA) were tested in simulated coal combustion and gasification atmospheres in a laboratory scale reactor. Arsenic and selenium compounds were retained to different extents on these carbons, retention efficiency depending mainly on the speciation of the element, which in turn depends on the gas atmosphere. Arsenic retention was similar in both combustion and gasification atmospheres unlike selenium retention. Moreover the retention of arsenic was lower than that of selenium.This work was carried out with the financial support of ECSC (7220-ED/095). We are also grateful to our colleagues in ICB (CSIC) R. Juan and C. Ruiz who prepared the CA activated carbon and Amelia Martínez Alonso of INCAR who assisted us in the textural characterization.Peer reviewe

The Effect of Method of Delivery and Psychosocial Factors on Postpartum Sexual Satisfaction

OBJECTIVE: The aim of this study was: 1) to determine factors associated with postpartum sexual satisfaction at 6 and 12 months postpartum; 2) to examine the effect of method of delivery on postpartum sexual satisfaction at 6 and 12 months postpartum. METHODS: This study undertook a secondary data analysis of The Ontario Mother and Infant Study (TOMIS) III which recruited 2560 postpartum women. Participants completed self-report in-hospital questionnaires and structured telephone interviews at 6 months and 12 months. RESULTS: Statistically significant factors associated with sexual satisfaction at 6 months postpartum were country of birth, breastfeeding status, physical health scores, perceived social support, mental health scores and the risk of postpartum depression. At 12 months postpartum, breastfeeding status, physical health scores, perceived social support and mental health scores were associated with postpartum sexual satisfaction. Method of delivery was not found to be statistically significant at both time points

Outlier Detection Methods for Industrial Applications

An outlier is an observation (or measurement) that is different with respect to the other values contained in a given dataset. Outliers can be due to several causes. The measurement can be incorrectly observed, recorded or entered into the process computer, the observed datum can come from a different population with respect to the normal situation and thus is correctly measured but represents a rare event. In literature different definitions of outlier exist: the most commonly referred are reported in the following: - "An outlier is an observation that deviates so much from other observations as to arouse suspicions that is was generated by a different mechanism " (Hawkins, 1980). - "An outlier is an observation (or subset of observations) which appear to be inconsistent with the remainder of the dataset" (Barnet & Lewis, 1994). - "An outlier is an observation that lies outside the overall pattern of a distribution" (Moore and McCabe, 1999). - "Outliers are those data records that do not follow any pattern in an application" (Chen and al., 2002). - "An outlier in a set of data is an observation or a point that is considerably dissimilar or inconsistent with the remainder of the data" (Ramasmawy at al., 2000). Many data mining algorithms try to minimize the influence of outliers for instance on a final model to develop, or to eliminate them in the data pre-processing phase. However, a data miner should be careful when automatically detecting and eliminating outliers because, if the data are correct, their elimination can cause the loss of important hidden information (Kantardzic, 2003). Some data mining applications are focused on outlier detection and they are the essential result of a data-analysis (Sane & Ghatol, 2006). The outlier detection techniques find applications in credit card fraud, network robustness analysis, network intrusion detection, financial applications and marketing (Han & Kamber, 2001). A more exhaustive list of applications that exploit outlier detection is provided below (Hodge, 2004): - Fraud detection: fraudulent applications for credit cards, state benefits or fraudulent usage of credit cards or mobile phones. - Loan application processing: fraudulent applications or potentially problematical customers. - Intrusion detection, such as unauthorized access in computer networks