Proteomic profiling of neuromas reveals alterations in protein composition and local protein synthesis in hyper-excitable nerves

Neuropathic pain may arise following peripheral nerve injury though the molecular mechanisms associated with this are unclear. We used proteomic profiling to examine changes in protein expression associated with the formation of hyper-excitable neuromas derived from rodent saphenous nerves. A two-dimensional difference gel electrophoresis ( 2D-DIGE) profiling strategy was employed to examine protein expression changes between developing neuromas and normal nerves in whole tissue lysates. We found around 200 proteins which displayed a > 1.75-fold change in expression between neuroma and normal nerve and identified 55 of these proteins using mass spectrometry. We also used immunoblotting to examine the expression of low-abundance ion channels Nav1.3, Nav1.8 and calcium channel alpha 2 delta-1 subunit in this model, since they have previously been implicated in neuronal hyperexcitability associated with neuropathic pain. Finally, S(35)methionine in vitro labelling of neuroma and control samples was used to demonstrate local protein synthesis of neuron-specific genes. A number of cytoskeletal proteins, enzymes and proteins associated with oxidative stress were up-regulated in neuromas, whilst overall levels of voltage-gated ion channel proteins were unaffected. We conclude that altered mRNA levels reported in the somata of damaged DRG neurons do not necessarily reflect levels of altered proteins in hyper-excitable damaged nerve endings. An altered repertoire of protein expression, local protein synthesis and topological re-arrangements of ion channels may all play important roles in neuroma hyper-excitability

Herida de bala del corazón

Presented at session 4th July 1956Presentado en sesión de 4 de julio de 195

Enhancing the Adult ADHD Diagnosis: Developing a Best Practice Protocol for the Psychiatric Outpatient Setting

Background: Although typically diagnosed in childhood, Attention-Deficit/Hyperactivity Disorder (ADHD) is increasingly recognized in adults. Current diagnostic criteria for adults mirror those for children, differing mainly in the number of criteria required. Despite established guidelines, many providers lack confidence in diagnosing adult ADHD accurately, often due to challenges in confirming symptom onset before age 12, concerns about malingering, and uncertainty about the rising prevalence. Educating providers and developing a standardized diagnostic protocol may increase confidence and improve diagnostic accuracy. Purpose: The purpose of this quality improvement project is to improve psychiatric providers’ diagnostic process of adult ADHD by implementing an evidence-based diagnostic protocol in a psychiatric outpatient setting. This protocol was developed through a scoping review that evaluated the validity, reliability, and accuracy of adult ADHD diagnostic tools to inform clinical recommendations. Methods: A scoping literature review was conducted to evaluate the validity, reliability, and accuracy of diagnostic tools for ADHD. Based on the findings, and evidence-based diagnostic protocol was developed and implements in a psychiatric outpatient clinic over a one-month period. Pre- and post-intervention surveys were used to assess provider confidence and satisfaction. Cohen’s d was used to analyze changes. Results: Following protocol implementation, provider confidence improved in diagnosing ADHD (d = 0.78) and differentiating it from other conditions (d = 0.87). However, confidence declined when diagnosing under time constraints (-0.55). Providers rated the protocol highly for clarity (M = 5.00; SD = 0) and accuracy (M = 5.00; SD = 0), though time efficiency (M = 2.00; SD = 0.82) and practicality (M = 2.25; SD = 1.26) received lower satisfaction ratings. Variability in confidence and satisfaction correlated with provider experience, with less experienced providers reporting greater gains. Conclusion: Implementing an evidence-based Adult ADHD Diagnostic Protocol enhanced provider knowledge and confidence, especially among less experienced clinicians. Despite strong ratings for clarity and accuracy, limited time efficiency hindered broader adoption. Future refinements should streamline the protocol and address patient engagement to improve usability and sustained clinical implementation

Picaduras por arañas del lino: a propósito de la comunicación del Dr. Carlos V. Stajano sobre "Lesiones cutáneas por picadura de insectos"

Presented at session 24th October 1945Presentado en sesión de 24 de octubre de 194

Invaginación intestinal por pólipo

Presented at the session of August 6, 1930Presentado en la sesión de 6 de agosto de 1930

EL PROGRAMA DE COOPERACIÓN INTERUNIVERSITARIA DE LA AECID: 18 AÑOS DE HISTORIA INTERRUMPIDOS POR LA CRISIS ECONÔMICA

Se acerca el verano y este año, por primera vez en dieciocho años, no saldrá la convocatoria del “Programa de Cooperación Interuniversitaria” más conocido por su acrónimo “PCI” de la “Agencia Española de Cooperación Internacional para el Desarrollo” (AECID). Este breve apunte, sólo quería dejar constancia de la cancelación de uno de los programas más exitosos y de más larga tradición de la cooperación española. Esperando que esta supresión sea sólo temporal y que, en un futuro no muy lejano pueda ser recuperado para no perder el saber hacer acumulado durante todos estos años por cientos de profesores y universidades del estado español

Fractura del astrágalo

Session of April 21st, 1938.Sesión del 21 de abril de 1938

Optical control of nanoparticle catalysis influenced by photoswitch positioning in hybrid peptide capping ligands

YesHere we present an in-depth analysis of structural factors that modulate peptide-capped nanoparticle catalytic activity via optically driven structural reconfiguration of the biointerface present at the particle surface. Six different sets of peptide-capped Au nanoparticles were prepared, in which an azobenzene photoswitch was incorporated into one of two well-studied peptide sequences with known affinity for Au, each at one of three different positions: The N- or C-terminus, or mid-sequence. Changes in the photoswitch isomerization state induce a reversible structural change in the surface-bound peptide, which modulates the catalytic activity of the material. This control of reactivity is attributed to changes in the amount of accessible metallic surface area available to drive the reaction. This research specifically focuses on the effect of the peptide sequence and photoswitch position in the biomolecule, from which potential target systems for on/off reactivity have been identified. Additionally, trends associated with photoswitch position for a peptide sequence (Pd4) have been identified. Integrating the azobenzene at the N-terminus or central region results in nanocatalysts with greater reactivity in the trans and cis conformations, respectively; however, positioning the photoswitch at the C-terminus gives rise to a unique system that is reactive in the trans conformation and partially deactivated in the cis conformation. These results provide a fundamental basis for new directions in nanoparticle catalyst development to control activity in real time, which could have significant implications in the design of catalysts for multistep reactions using a single catalyst. Additionally, such a fine level of interfacial structural control could prove to be important for applications beyond catalysis, including biosensing, photonics, and energy technologies that are highly dependent on particle surface structures.Air Office of Scientific Research, grant number FA9550-12- 1-0226

Síndrome de Sjgren. Presentacion de un caso

El rasgo distintivo del Sindrome de Sjgren (SS) es el complejo de Sindrome Seco (xerostalmia y xerostoma) lo que encontramos en la paciente que estudiamos, asÍcomo la asociaciÓn de un fibromioma uterino como hallazgo al exÁmen clÍnico. Se logra determinar histologicamente las alteraciones de dicha entidad