ブドウの芽の休眠打破に及ぼす化学物質の種類と濃度の影響

　The effects of various chemicals and their concentrations on budbreak of ‘Pione’ grapevine (Vitis labrusca L. x V. vinifera L.) were studied by using single-bud cuttings obtained in endodormancy. When seven chemicals were applied to the upper half of cuttings, including bud, 2% hydrogen cyanamide (H2CN2) was most effective in budbreak, judging from acceleration and uniformity of budbreak. However, neither 10 % suspension of calcium cyanamide (CaCN2) nor 5% diallyl disulfide (C6H10S2) had any effect in breaking bud dormancy of ‘Pione’ cuttings. Budbreak in cuttings treated with 10 % hydrogen peroxide (H2O2) was inhibited slightly compared to the control cuttings. No effect of 2% potassium chlorate (KClO3), 2% sodium chlorate (NaClO3) or 2% paclobutrazol (PBZ) on breaking bud dormancy in ‘Pione’ cuttings was observed. The effects of CaCN2, H2CN2 and C6H10S2 on breaking bud dormancy in ‘Pione’ cuttings were compared at three to four concentrations. With CaCN2, a 20 % suspension significantly promoted budbreak, but a 5% suspension resulted in no effect. Both 5% and 2% of H2CN2 accelerated budbreak significantly and resulted in uniform budbreak, especially at 5 %, whereas at 0.5% H2CN2 no effect was observed. Of three concentration of C6H10S2, only a 10 % solution showed any effectiveness in budbreak. The results indicated that H2CN2 is most effective in breaking bud dormancy of ‘Pione’ grapevine cuttings, followed by CaCN2 and C6H10S2 in that order, although their effectiveness varied largely according to the concentrations for all chemicals.自発休眠期に採取したブドウ'ピオーネ'の挿し穂を用い，芽の休眠打破に及ぼす化学物資の種類と濃度の影響を調査した．７種類の化学物質について休眠打破の効果を比較したところ，発芽の促進と揃いからみて，２%シアナミド （H2CN2）の効果が最も大きかった．一方，10%石灰窒素（CaCN2）と５%２ 硫化ジアリル（C6H10S2）には休眠打破の効果は認められなかった．10%過酸化水素（H2O2）では発芽がやや抑制された．２%の塩素酸カリウ（KClO3）， 塩素酸ナトリウム（NaClO3）およびパクロブトラゾール（PBZ）はいずれも休眠打破の効果を示さなかった．石灰窒素，シアナミドおよび２硫化ジアリルの濃度を３～４段階に変え，'ピオーネ' 挿し穂の休眠打破に及ぼす影響を調査した．石灰窒素では，20%区の発芽が著しく促進されたが，５ｵ 区では発芽の揃いがよくなかった．シアナミドでは，５%区と２%区の休眠打破効果が著しかったのに対し，0.5%区では休眠打破の効果は認められなかった．２硫 化ジアリルでは，10%区で休眠打破効果がみられただけであった．以上の結果から，ブドウの芽の休眠打破に及ぼす化学物質の効果はシアナミドで最も大きく，次いで石灰窒素，２硫化ジアリルの順であったが，効果の程度は濃度によって異なった

休眠程度の異なるブドウ‘ピオーネ’の発芽に及ぼす温度の影響

The effects of temperature on budbreak of cuttings obtained at different stages of dormancy from 'Pione' grapevines (Vitis labrusca × V. vinifera) grown in open field were investigated. Cuttings were collected at monthly intervals from July to March. Judging from the number of days to initial and 60% budbreak after treatment, indicating promotion and the uniformity of budbreak, respectively, 30℃ was the most effective in budbreak, followed by 25 and 20℃ in that order in all treatment times. However, the effect of temperature on budbreak was markedly affected by treatment time. The number of days to initial budbreak (NDIB) increased gradually from July to October, peaked in December and thereafter decreased gradually towards March. The periods from July to September, from October to December, and from January to March were assumed to correspond to paradormancy, endodormancy, and ecodormancy of 'Pione' grapevines, respectively. Final percentage of budbreak was less than 100% until endodormancy for all temperatures. It was below 60% at 20℃ treatments of July to September. On the other hand, a uniform budbreak was observed in the treatments after the middle of endodormancy for all temperatures, resulting in almost 100% of final percentage of budbreak. There was a significant negative correlation between NDIB and cumulative chilling hour (CCH) of exposure to below 7.2℃ in the treatments after November, and also between NDIB and cumulative temperature (CT, ℃･h), a summation of temperature and hours of exposure to above 0°C from November 1 to each treatment time and hours of exposure to 20, 25, or 30℃ from start of treatment to budbreak in each plot. The results suggest that besides CCH, CT can also be used to estimate the completion of dormancy in 'Pione' grapevine bud.露地栽培されているブドウ‘ピオーネ’について，休眠の深さが異なる7月から翌年3月まで約1か月間隔で枝を採取し，1芽を有す挿し穂を調整した後，20，25および30℃に制御したインキュベーター（いずれも14時間日長）に入れ，経時的に発芽を調査した．発芽の早さを示す発芽所要日数と発芽の揃いを示す60%発芽所要日数から発芽に及ぼす温度の影響を評価した．実験期間中の温度を測定し，休眠完了と温度との関係を考察した．いずれの処理時期においても30℃の発芽が最も優れ，次いで25℃，20℃の順であった．しかし，発芽に及ぼす温度の影響は処理時期によって大きく異なった．すなわち，発芽所要日数は7月から10月までは徐々に増加し，11月に最大に達した後，3月に向けて少しずつ減少した．このことから，‘ピオーネ’では7月から9月が条件的休眠期，10月から12月が自発休眠期，1月から3月が他発休眠期と推察された．自発休眠期までの最終発芽率はいずれの温度も100%未満であり，また7月～9月の20℃処理では60%未満の発芽率であった．一方，自発休眠期の中期以降の処理ではいずれの温度とも均一な発芽を示し，最終発芽率はほぼ100%であった．11月以降の処理において，発芽所要日数と7.2℃以下の温度に遭遇した時間数（CCH）との間に有意な負の相関があった．また，11月１日から各処理時期までの0℃以上の温度に遭遇した時間数と20，25または30℃で処理を始めた日から各処理区の発芽までの時間数との積算（CT, ℃・h）との間にも有意な負の相関が認められた．以上のことから，‘ピオーネ’の芽の休眠完了の予測には低温遭遇量だけでなく，0℃以上の積算温度による方法も有効と考えられた

ブドウの芽の休眠打破に伴う生理的変化

Changes in CO2 and C2H4 production and water content of bud associated with breaking in ‘Pione’ grapevine (Vitis labrusca ×V. vinifera) were investigated throughout dormancy. Buds were collected monthly from August to December, during dormancy induction and maintenance, and CO2 and C2H4 production were determined by GC after incubation. Both CO2 and C2H4 production, especially for the latter, were low throughout the experiment. Water content of bud gradually increased until October ; thereafter it was constant. When CO2 and C2H4 production was determined from December to April, during dormancy maintenance to release, CO2 production was low from beginning of experiment to early April, prior to bursting, then rapidly increased to April 13, the bursting date. C2H4 production was almost undetectable throughout the experiment. Cuttings obtained at 3 different stages of dormancy were applied with 2% H2CN2 or distilled water (control), and budbreak was monitored in a plastic house kept at 20℃ or more. The CO2 and C2H4 production of bud were also determined weekly until budbreak. Regardless of treatment time H2CN2 significantly promoted budbreak compared to the control. Significantly higher production of CO2 was observed in cuttings treated with H2CN2 at 3 to 9 days before bursting for all the treatment times. C2H4 production was very low throughout the experiment for all the treatments. Irrespective of chemical application and treatment time, water content of bud decreased to the bursting stage, H2CN2 treatment especially showing a large decline. When dormant cuttings were treated with ACC, GSH (reduced glutathione) and GSSG (oxidized glutathione), only ACC promoted budbreak. Budbreak in cuttings treated with cyanamides such as CaCN2 and H2CN2 and cyanides such as KCN and NaCN was significantly accelerated except for H2CN2. Based on these results, the relationship between budbreak of grapevine buds and physiological changes in buds, and the roles of substances related to ethylene biosynthesis on breaking bud dormancy are discussed.ブドウ‘ピオーネ’について，休眠の導入と覚醒の過程における芽の生理的変化を調査した．休眠導入期の8月から覚醒初期の12月まで露地で栽培されている個体から芽を採取し，呼吸量，エチレン生成量および含水率を測定し た．調査期間を通して呼吸量は低く，エチレンもほとんど検出されなかった．芽の含水率は8月から10月まで僅かに上昇し，その後は変化がみられなかった．休眠覚醒初期の12月から発芽期の4月中旬まで，芽の呼吸量とエチレン生成量を測定した．呼吸量は4月上旬までは低く推移し，発芽（4月13日）の直前に急上昇した．エチレンは測定期間を通して低かった．休眠期の12月，1月および2月に採取した穂木を2% H2CN2 または蒸留水（対照）で処理し，25℃以上に保ったプラスチックハウスに入れて発芽を調査するとともに，経時的に芽の呼吸量，エチレン生成量および含水率を測定した．両時期とも対照区よりも H2CN2 処理区の発芽が早く，しかも休眠の深い12月処理で区による差が大きかった．両区いずれの時期とも，芽の呼吸量は発芽直前に急上昇したのに対し，エチレン生成量は調査期間を通して低いままであった．芽の含水率は，いずれの時期および処理区とも発芽期に低下し，特に H2CN2 処理区の低下が大きかった．休眠最深期の10月に採取した穂木に ACC，GSH（還元型グルタチオン）および GSSG（酸化型グルタチオン）を処理し，発芽に及ぼす影響を調査したところ，ACC だけが発芽を促進した．同様に，4種のシアン化合物（CaCN2，H2CN2，KCN，NaCN）を処理したところ，H2CN2を除き有意に発芽を促した．これらの結果を基に，ブドウの発芽と生理的変化との関係および休眠覚醒に及ぼすエチレン生合成関連物質の作用性について考察した

Cervical radiculopathy: Incidence and treatment of 1,420 consecutive cases

Study DesignRetrospective case series.PurposeTo determine the incidence of cervical radiculopathy requiring operative intervention by level and to report on the methods of treatment.Overview of LiteratureCervical radiculopathy is a common cause of pain and can result in progressive neurological deficits. Although the pathology is well understood, the actual incidence of cervical radiculopathy at particular spinal levels ultimately requiring operative intervention is unknown.MethodsA large consecutive series of patients operated on by a single surgeon were retrospectively analyzed. The incidence of cervical radiculopathy at each level was defined for every patient. Procedures used for operative treatment were noted. Health related quality of life (HRQL) scores were collected both pre-operatively and postoperatively.ResultsThere were 1305 primary and 115 revision operations performed. The most common primary procedures performed were anterior cervical discectomy and fusion (ACDF, 50%) and anterior cervical corpectomy and fusion (ACCF, 28%). The most commonly affected levels were C6 (66%) and C7 (62%). Reasons for revision were pseudarthrosis (27%), clinical adjacent segment pathology (CASP, 63%), persistent radiculopathy (11%), and hardware-related (2.6%). The most common procedures performed in the revision group were posterior cervical decompression and fusion (PCDF, 42%) and ACDF (40%). The most commonly affected levels were C7 (43%) and C5 (30%). Among patients that had their index surgery at our institution, the revision rate was 6.4%. In both primary and revision cases there was a significant improvement in Neck Disability Index and visual analogue scale scores postoperatively. Postoperative HRQL scores in the revision cases were significantly worse than those in the primary cases (p <0.01).ConclusionsThis study provides the largest description of the incidence of cervical radiculopathy by level and operative outcomes in patients undergoing cervical decompression. The incidence of CASP was 4.2% in 3.3 years in this single institution series

Dissemination of clonal Salmonella enterica serovar Typhimurium isolates causing salmonellosis in Mauritius

Salmonella enterica serotype Typhimurium is one of the leading causes of salmonellosis in Mauritius, where it has also been associated with outbreaks of foodborne illness. However, little is known about its molecular epidemiology in the country. This study was therefore undertaken to investigate the clonality and source of Salmonella Typhimurium in Mauritius by studying human, food, and poultry isolates by pulsed-field gel electrophoresis (PFGE) and antibiotic minimum inhibitory concentration determination. Forty-nine isolates collected between 2008 and 2011 were analyzed, including 25 stool isolates from foodborne illness outbreaks and sporadic gastroenteritis cases, four blood isolates, one postmortem colon isolate, 14 food isolates, and five poultry isolates. All isolates were pansusceptible to the 16 antibiotics tested, except for two isolates that were resistant to sulfamethoxazole and trimethoprim. Overall characterization of the isolates by PFGE digested with XbaI and BlnI resulted in eight different patterns. The largest of the clusters in the composite dataset consisted of 20 isolates, including two raw chicken isolates, four poultry isolates, and nine human stool isolates from two outbreaks. A second cluster consisted of 18 isolates, of which 12 originated from human blood and stool samples from both sporadic and outbreak cases. Six food isolates were also found in this cluster, including isolates from raw and grilled chicken, marlin mousse, and cooked pork. One poultry isolate had a closely related PFGE pattern. The results indicate that one clone of Salmonella Typhimurium found in poultry has been causing outbreaks of foodborne illness in Mauritius and another clone that has caused many cases of gastrointestinal illness and bacteremia in humans could also be linked to poultry. Thus, poultry appears to be a major reservoir for Salmonella Typhimurium in Mauritius. Initiating on-farm control strategies and measures against future dissemination may substantially reduce the number of cases of salmonellosis in the country

Characterization of Salmonella enterica serovar Enteritidis isolates recovered from blood and stool specimens in Thailand

<p>Abstract</p> <p>Background</p> <p>Bacteremia due to <it>Salmonella</it> spp. is a life-threatening condition and is commonly associated with immune compromise. A 2009 observational study estimated risk factors for the ten most common non-typhoidal <it>Salmonella</it> (NTS) serovars isolated from Thai patients between 2002–2007. In this study, 60.8% of <it>Salmonella enterica</it> serovar Enteritidis isolates (n = 1517) were recovered from blood specimens and infection with <it>Salmonella</it> serovar Enteritidis was a statistically significant risk factor for bacteremia when compared to other NTS serovars. Based on this information, we characterized a subset of isolates collected in 2008 to determine if specific clones were recovered from blood or stool specimens at a higher rate. Twenty blood isolates and 20 stool isolates were selected for antimicrobial resistance testing (MIC), phage typing, PFGE, and MLVA.</p> <p>Result</p> <p>Eight antibiogrammes, seven MLVA types, 14 <it>Xba</it>I/<it>Bln</it>I PFGE pattern combinations, and 11 phage types were observed indicating considerable diversity among the 40 isolates characterized. Composite analysis based on PFGE and MLVA data revealed 22 genotypes. Seven of the genotypes containing two or more isolates were from both stool and blood specimens originating from various months and zones. Additionally, those genotypes were all further discriminated by phage type and/or antibiogramme. Ninety percent of the isolates were ciprofloxacin resistant.</p> <p>Conclusions</p> <p>The increased percentage of bloodstream infections as described in the 2009 observational study could not be attributed to a single clone. Future efforts should focus on assessing the immune status of bacteriaemic patients and identifying prevention and control measures, including attribution studies characterizing non-clinical (animal, food, and environmental) isolates.</p

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