Research on borehole gas extraction based on the influence of mining height on the evolution of overburden fracture in working face

The evolution of fractures in mining overburden plays a pivotal role in interlayer gas migration. Therefore, a comprehensive study of crack development and distribution improve the efficiency of gas extraction. This study the west 2107 fully mechanized mining face in Weijia Mine of Gansu Province as the engineering background, Specifically, this study established numerical models of different mining heights by using the UDEC simulation software, and analyzed the relationship between the evolution of near-field fractures in the overburden of the working face and different mining based on fractal geometry theory. The optimal working face height for gas extraction was determined and validated through theoretical empirical formulas, field observations of overburden fractures, and gas extraction results. The results show that the total number of mining fractures increases with the increase of mining height, but the amplitude of increase is different at different stages, and the overburden fractures continue to develop in an "umbrella" shape around the overburden. The near-field fractures of the overburden rock at different mining heights have undergone three stages: generation, expansion and penetration, and compaction stability. Increasing mining height, smaller compaction zones. When the mining height is 4 m, the development density and range of roof fractures at 10 m and 40 m are significantly higher than those in other areas, which is consistent with the optimal position of the collaborative extraction roadway with high and low roadways

N6-methyladenosine RNA modification promotes viral genomic RNA stability and infection

Molecular manipulation of susceptibility (S) genes that are antipodes to resistance (R) genes has been adopted as an alternative strategy for controlling crop diseases. Here, we show the S gene encoding Triticum aestivum m(6)A methyltransferase B (TaMTB) is identified by a genome-wide association study and subsequently shown to be a positive regulator for wheat yellow mosaic virus (WYMV) infection. TaMTB is localized in the nucleus, is translocated into the cytoplasmic aggregates by binding to WYMV NIb to upregulate the m(6)A level of WYMV RNA1 and stabilize the viral RNA, thus promoting viral infection. A natural mutant allele TaMTB-SNP176C is found to confer an enhanced susceptibility to WYMV infection through genetic variation analysis on 243 wheat varieties. Our discovery highlights this allele can be a useful target for the molecular wheat breeding in the future

Regional economic development and the choice of tourism mode

Tourism has become an important part of regional economic development and the choice of tourism mode has been an important factor affecting the development of tourism. From the perspective of revenue maximization, this paper constructs a selection model of ticket economy model and region-wide tourism model. Each tourist area has a threshold value of scenic spots. When the number of scenic spots exceeds the threshold value, it is more beneficial for regional development to choose the region-wide tourism mode; conversely, it is more beneficial for economic development to choose the ticket economy mode. Among the factors affecting the threshold value, there is a negative correlation between the threshold value and tourists' consumption in the scenic spot, the incremental effect of tourists brought by the cancellation of tickets, the distance between the scenic spot and the tourist source, the distance between scenic spots within the region and the diversity index of scenic spot business items. There is a positive correlation between ticket revenue, traffic conditions between tourist areas and tourist sources, traffic conditions in tourist areas and the level of scenic spots in the region

Abstract 4103: Knockdown of Notch1 inhibits tumor cell proliferation, migration, and invasion and reverses epithelial-mesenchymal transition (EMT) in nasopharyngeal carcinoma

Abstract Despite a large bodies of evidences support an oncogenic role of Notch signaling in several human solid cancers, however, the pleiotropic effects and molecular mechanisms of blockage of Notch signaling on nasopharyngeal carcinoma (NPC) remain unclear. In this study, Real-time RT-PCR and western blotting were firstly used to examine the expression of Notch1 receptor in a panel of NPC cell lines. CNE1 and CNE2 cells expressed a higher level of Notch1 compared to HONE1, SUNE1 and HK cells. Then Notch1 expression was specifically knocked down in CNE1 and CNE2 cells by Notch1 short hairpin RNA (shRNA). This resulted in a significant inhibition of cell proliferation, migration, and invasion; and interestingly, epithelial-mesenchymal transition (EMT) was reversed in these Notch1-transfected cells along with demonstration of epithelialioid-like morphology change and increase of E-cadherin and decrease of Vimentin expressions. In addition, targeted knockdown of Notch1 dramatically inhibited expression of urokinase plasminogen activator (uPA) and its receptor uPAR, chemokine CCL2 and CXCL16 indicating that these factors are downstream targets of Notch1. Finally, knockdown of Notch1 significantly diminished CNE1 cell growth in a murine model concomitant with inhibition of cell proliferation and induction of apoptosis. Taken together, these results suggest Notch1 may become a therapeutic target in nasopharyngeal carcinoma. Note: This abstract was not presented at the meeting. Citation Format: Huajiao Guo, Yi Lu, Chaonan Qian, Jian Zhang. Knockdown of Notch1 inhibits tumor cell proliferation, migration, and invasion and reverses epithelial-mesenchymal transition (EMT) in nasopharyngeal carcinoma. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 4103. doi:10.1158/1538-7445.AM2015-4103</jats:p

Effect of strontium dioxide on the crystal structure and properties of tricalcium silicate

Tricalcium silicate (C3S) doped with strontium dioxide was sintered at high temperature of 1600°C by solid reaction. The crystal structure and properties of C3S doped with strontium dioxide were investigated by means of chemical analysis, X-ray diffraction analysis, infrared analysis and hydration heat analysis. The experimental results show that pure C3S crystal has a triclinic crystalline form at room temperature. When doped strontium dioxide concentration is no more than 0·025 M, alite crystal is the same as the pure C3S crystalline form, whereas when doped strontium dioxide concentration is in the range 0·025–0·075 M, alite crystal is in monoclinic crystalline form. A small amount of strontium dioxide has the effect of decreasing the free lime content and promoting the formation of alite. Infrared analysis indicates that the addition of strontium dioxide can improve the crystal symmetry of alite. The hydration heat analysis shows that the addition of strontium dioxide can enhance hydration activity of alite and accelerate the hydration process. </jats:p

Key Genes Associated with Tumor-Infiltrating Non-regulatory CD4- and CD8-Positive T Cells in Microenvironment of Hepatocellular Carcinoma

AbstractThe immune microenvironment in hepatocellular carcinoma (HCC), especially T-cell infiltration, plays a key role in the prognosis and drug sensitivity of HCC. Our study aimed to analyze genes related to non-regulatory CD4+and CD8+T cell in HCC. Data of HCC samples were downloaded from The Cancer Genome Atlas (TCGA) database. According to stromal and immune score retrieved by Estimation of Stromal and Immune cells in Malignant Tumor tissues using Expression data (ESTIMATE) algorithm, differentiated expressed genes (DEGs) between high and low stromal/immune scoring groups were collected. Using Cibersort algorithm, abundance of immune cells was calculated and genes related with CD4+and CD8+T cells were selected. Protein–protein interaction (PPI) networks and networks of microRNA (miRNA)–target gene interactions were illustrated, in which CD4+and CD8+T cell-related core genes were selected. Finally, Cox regression test and Kaplan–Meier (K–M) survival analysis were conducted. Totally, 1579 DEGs were identified, where 103 genes and 407 genes related with CD4+and CD8+T cell were selected, respectively. Each of 30 core genes related to CD4+T cells and CD8+T cells were selected by PPI network. Four genes each related with the two types of T cells had a significant impact on prognosis of HCC patients. Amongst, KLRB1 and IL18RAP were final two genes related to both two kinds of T cells and associated with overall survival of the HCC patients.</jats:p