Un modèle débit-durée-fréquence pour caractériser le régime d'étiage d'un bassin versant

La méthodologie débit-durée-fréquence (QdF), appliquée ces dernières années aux étiages, a permis de définir quatre modèles types recouvrant l'ensemble des rivières étudiées. L'identification de la typologie du site étudié et l'estimation de deux descripteurs hydrologiques locaux suffisent au modèle, dit de référence, pour en déduire les courbes QdF (1j ≤ d ≤ 30j) en débit moyen minimum sur l'année (VCNd) ou débit seuil minimum annuel (QCNd) non dépassé sur ces mêmes durées. S'il est relativement aisé de définir les descripteurs hydrologiques, il est plus difficile d'identifier, sans observation de débit, le modèle à prendre en compte. En reconsidérant avec plus de rationalité la démarche d'identification des typologies, et en particulier les distributions multidurées relatives à chaque bassin, il est apparu possible d'évoluer vers un modèle unique pouvant être calé en chaque site observé. Ce nouveau concept de modélisation repose sur la propriété d'affinité des distributions, relatives aux échantillons de valeurs de durées d. Par souci de continuité avec l'approche QdF à référence typologique, la loi statistique log-normale à deux paramètres a été choisie. Le modèle, dont la conceptualisation est indépendante de la loi statistique choisie, aura dans le cas de la loi log-normale trois paramètres à ajuster sur les VCNd échantillonnés. Trente six sous bassins du bassin hydrographique de la Moselle ont été étudiés. Le modèle développé pour les débit moyens VCNd peut être appliqué aux débits seuils QCNd en conservant le même jeu de paramètres, grâce à une relation observée entre débits moyens et débits seuils. Cette nouvelle modélisation rationalise l'approche antérieure basée sur la typologie d'écoulement de basses eaux des bassins versants.The flow-duration-frequency (QdF) concept, as applied in recent years to low flows, has made it possible to establish four reference models (GALEA et al., 1999a), corresponding to four typologies. The hydrological variables concerned are the minimum mean discharge of the year defined for various continuous durations d (1day ≤ d ≤ 30day), called VCNd, and the annual minimum threshold discharge not exceeded over these same durations, called QCNd, according to OBERLIN (1992). These QdF models allow a description of the temporal variability of low flows observed for a river basin, from a statistical point of view. The typology of the basin and two local hydrological descriptors have to be known. For ungauged basins, these two descriptors (GALEA et al., 1999b) are well estimated by various methods, such as multivariate analysis relating to the physiographic characteristics of the basin. Nevertheless, the choice of the reference model still remains contentious.By reconsidering in a more rational manner the step of identification of typologies, and in particular the discharge distributions (for durations d) relating to each basin, it appeared interesting to establish a local model. This new model has a simpler formulation, thanks to a scale invariance assumption. This research (CHAPUT, 1999) was undertaken on 36 sub-basins of the Mosel basin. In order to ensure continuity with the earlier QdF models described above, the two-parameter log-normal law was chosen and adjusted on the distribution of mean discharges. The scale invariance assumption is deduced from the observed parallelism of distributions related to different durations, when discharges are represented in a logarithm scale. This observation means that all of the distributions can be translated to a common point, in order to obtain one "consolidated" distribution, independent on the considered duration. This parallelism has been observed on many basins, and seems to be a realistic assumption. Furthermore, these observations have been made on samples, and do not depend on the choice of statistical law. The methodology described in this paper makes it possible to adjust the local QdF model on sampled discharges. Only three parameters have to be determined: sc, the "consolidated" standard deviation, ∆e the low flow characteristic duration and VCN(2,1), which represents the quantile of the one-day distribution, with the two-year return period (F=0.5).This model is also useful for the determination of threshold discharges (QCNd). An observed property gives a relation between the VCN and QCN quantiles, for a fixed return period, considering different durations d: VCN quantiles can been deduced from QCN quantiles by integrating them, according to d. Consequently, the analytical formulation of the VCN model can be derived according to d, in order to obtain a QCN model. This model has the same three parameters sc, ∆e and VCN(2,1) described above. The comparison between QCN quantiles adjusted on samples and QCN quantiles deduced from the VCN model by derivation shows good results.As a conclusion, this new modelling approach unifies the typological approach for both mean discharges and threshold discharges. It is based on a local adjustment and avoids having to choose between one of the four former reference models. This local model opens up perspectives for a regional model, as it has been done for floods, for example by the Group of Research in Statistical Hydrology (1996). This will make it possible to estimate the low flow regime on an ungauged basin

Droit de la construction : de la jurisprudence à la pratique notariale

"Parce que le contentieux de la construction concerne les notaires à bien des égards, cette nouvelle chronique, rédigée par des universitaires et des praticiens, a pour objectif premier de brosser un panorama des décisions importantes rendues au cours de l\u27année 2012 en la matière. Les signataires de ces lignes ont ensuite cherché, dans le cadre de cette collaboration entre chercheurs et notaires, à enrichir leurs commentaires de conseils pratiques. Ainsi, au cours de l\u27année écoulée, la Cour de cassation s\u27est prononcée sur des questions générales d\u27organisation de l\u27immeuble, précisant la liberté de choix entre le statut de la copropriété et la division en volumes ou revenant sur les règles applicables aux travaux réalisés sur un mur mitoyen. Des précisions ont également été apportées sur le champ d\u27application de la responsabilité décennale des constructeurs, puisqu\u27il a été rappelé que le vendeur même non professionnel d\u27un immeuble rénové peut, à certaines conditions, engager sa responsabilité décennale, de même que cette cause de responsabilité est également susceptible de s\u27appliquer lorsque les dommages affectant un immeuble neuf causent des préjudices à des tiers ou se matérialisent par un simple risque d\u27écroulement, sans que la catastrophe ne soit encore survenue. Un arrêt important précise en outre, dans le domaine des assurances, la distinction entre la faute lourde, couverte, et celle intentionnelle, dont l\u27assureur ne répondra pas. On relèvera finalement trois décisions importantes en matière de vente d\u27immeubles à construire. Deux concernent la rédaction des actes, quant à la distinction entre les notions d\u27achèvement et de livraison d\u27une part, puis relativement aux clauses d\u27allongement de délai de livraison pour intempéries, d\u27autre part. Le dernier revient sur la question importante la rénovation en précisant l\u27étendue de la responsabilité du notaire dans le choix entre le modèle de la vente en l\u27état futur d\u27achèvement et celui de la vente d\u27immeuble à rénover." Gwenaëlle Durand-Pasquie

Non-monotonic field-dependence of the ZFC magnetization peak in some systems of magnetic nanoparticles

We have performed magnetic measurements on a diluted system of gamma-Fe2O3 nanoparticles (~7nm), and on a ferritin sample. In both cases, the ZFC-peak presents a non-monotonic field dependence, as has already been reported in some experiments,and discussed as a possible evidence of resonant tunneling. Within simple assumptions, we derive expressions for the magnetization obtained in the usual ZFC, FC, TRM procedures. We point out that the ZFC-peak position is extremely sensitive to the width of the particle size distribution, and give some numerical estimates of this effect. We propose to combine the FC magnetization with a modified TRM measurement, a procedure which allows a more direct access to the barrier distribution in a field. The typical barrier values which are obtained with this method show a monotonic decrease for increasing fields, as expected from the simple effect of anisotropy barrier lowering, in contrast with the ZFC results. From our measurements on gamma-Fe2O3 particles, we show that the width of the effective barrier distribution is slightly increasing with the field, an effect which is sufficient for causing the observed initial increase of the ZFC-peak temperatures.Comment: LaTeX file 19 pages, 9 postscript figures. To appear in Phys. Rev. B (tentative schedule: Dec.97

Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes

Introduction: In recent years, there has been an exponential increase in the number of studies aiming to understand the biology of exosomes, as well as other extracellular vesicles. However, classification of membrane vesicles and the appropriate protocols for their isolation are still under intense discussion and investigation. When isolating vesicles, it is crucial to use systems that are able to separate them, to avoid cross-contamination. Method: EVs released from three different kinds of cell lines: HMC-1, TF-1 and BV-2 were isolated using two centrifugation-based protocols. In protocol 1, apoptotic bodies were collected at 2,000×g, followed by filtering the supernatant through 0.8 µm pores and pelleting of microvesicles at 12,200×g. In protocol 2, apoptotic bodies and microvesicles were collected together at 16,500×g, followed by filtering of the supernatant through 0.2 µm pores and pelleting of exosomes at 120,000×g. Extracellular vesicles were analyzed by transmission electron microscopy, flow cytometry and the RNA profiles were investigated using a Bioanalyzer®. Results: RNA profiles showed that ribosomal RNA was primary detectable in apoptotic bodies and smaller RNAs without prominent ribosomal RNA peaks in exosomes. In contrast, microvesicles contained little or no RNA except for microvesicles collected from TF-1 cell cultures. The different vesicle pellets showed highly different distribution of size, shape and electron density with typical apoptotic body, microvesicle and exosome characteristics when analyzed by transmission electron microscopy. Flow cytometry revealed the presence of CD63 and CD81 in all vesicles investigated, as well as CD9 except in the TF-1-derived vesicles, as these cells do not express CD9. Conclusions: Our results demonstrate that centrifugation-based protocols are simple and fast systems to distinguish subpopulations of extracellular vesicles. Different vesicles show different RNA profiles and morphological characteristics, but they are indistinguishable using CD63-coated beads for flow cytometry analysis

Expression of Foxp3 in colorectal cancer but not in Treg cells correlates with disease progression in patients with colorectal cancer

Background: Regulatory T cells (Treg) expressing the transcription factor forkhead-box protein P3 (Foxp3) have been identified to counteract anti-tumor immune responses during tumor progression. Besides, Foxp3 presentation by cancer cells itself may also allow them to evade from effector T-cell responses, resulting in a survival benefit of the tumor. For colorectal cancer (CRC) the clinical relevance of Foxp3 has not been evaluated in detail. Therefore the aim of this study was to study its impact in colorectal cancer (CRC). Methods and Findings: Gene and protein analysis of tumor tissues from patients with CRC was performed to quantify the expression of Foxp3 in tumor infiltrating Treg and colon cancer cells. The results were correlated with clinicopathological parameters and patients overall survival. Serial morphological analysis demonstrated Foxp3 to be expressed in cancer cells. High Foxp3 expression of the cancer cells was associated with poor prognosis compared to patients with low Foxp3 expression. In contrast, low and high Foxp3 level in tumor infiltrating Treg cells demonstrated no significant differences in overall patient survival. Conclusions: Our findings strongly suggest that Foxp3 expression mediated by cancer cells rather than by Treg cells contribute to disease progression

Lorenz function of Bi2_{2}Te3_{3}/Sb2_{2}Te3_{3} superlattices

Combining first principles density functional theory and semi-classical Boltzmann transport, the anisotropic Lorenz function was studied for thermoelectric Bi$_{2}$Te$_{3}$/Sb$_{2}$Te$_{3}$ superlattices and their bulk constituents. It was found that already for the bulk materials Bi$_{2}$Te$_{3}$ and Sb$_{2}$Te$_{3}$, the Lorenz function is not a pellucid function on charge carrier concentration and temperature. For electron-doped Bi$_{2}$Te$_{3}$/Sb$_{2}$Te$_{3}$ superlattices large oscillatory deviations for the Lorenz function from the metallic limit were found even at high charge carrier concentrations. The latter can be referred to quantum well effects, which occur at distinct superlattice periods

Distribution-based bisimulation for labelled Markov processes

In this paper we propose a (sub)distribution-based bisimulation for labelled Markov processes and compare it with earlier definitions of state and event bisimulation, which both only compare states. In contrast to those state-based bisimulations, our distribution bisimulation is weaker, but corresponds more closely to linear properties. We construct a logic and a metric to describe our distribution bisimulation and discuss linearity, continuity and compositional properties.Comment: Accepted by FORMATS 201

Learning object relationships which determine the outcome of actions

Peer reviewedPublisher PD

The Biomolecule Sequencer Project: Nanopore Sequencing as a Dual-Use Tool for Crew Health and Astrobiology Investigations

Human missions to Mars will fundamentally transform how the planet is explored, enabling new scientific discoveries through more sophisticated sample acquisition and processing than can currently be implemented in robotic exploration. The presence of humans also poses new challenges, including ensuring astronaut safety and health and monitoring contamination. Because the capability to transfer materials to Earth will be extremely limited, there is a strong need for in situ diagnostic capabilities. Nucleotide sequencing is a particularly powerful tool because it can be used to: (1) mitigate microbial risks to crew by allowing identification of microbes in water, in air, and on surfaces; (2) identify optimal treatment strategies for infections that arise in crew members; and (3) track how crew members, microbes, and mission-relevant organisms (e.g., farmed plants) respond to conditions on Mars through transcriptomic and genomic changes. Sequencing would also offer benefits for science investigations occurring on the surface of Mars by permitting identification of Earth-derived contamination in samples. If Mars contains indigenous life, and that life is based on nucleic acids or other closely related molecules, sequencing would serve as a critical tool for the characterization of those molecules. Therefore, spaceflight-compatible nucleic acid sequencing would be an important capability for both crew health and astrobiology exploration. Advances in sequencing technology on Earth have been driven largely by needs for higher throughput and read accuracy. Although some reduction in size has been achieved, nearly all commercially available sequencers are not compatible with spaceflight due to size, power, and operational requirements. Exceptions are nanopore-based sequencers that measure changes in current caused by DNA passing through pores; these devices are inherently much smaller and require significantly less power than sequencers using other detection methods. Consequently, nanopore-based sequencers could be made flight-ready with only minimal modifications