RETRACTED ARTICLE: Growth inhibition and apoptosis induced by 6-fluoro-3-formylchromone in hepatocellular carcinoma

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most lethal and prevalent cancers in human population. The 6-fluoro-3-formylchromone (FCC) has been shown to have anti-tumor activity against various tumor cells. However, the effects of FCC on HCC cell lines have not yet been reported. This study aims to research the effects of FCC on HCC and advance the understanding of the molecular mechanism. METHODS: HCC cell line SMMC-7721 was treated with FCC at various concentrations (0, 2, 5, 10, and 20 μg/ml) for 24, 48 and 72 h, respectively. The proliferations of SMMC-7721 cells were measured by MTT assays. After cultured 24 hours, cell cycle distribution and apoptosis were determined by flow cytometry. However, the expression levels of PCNA, Bax and Bcl-2 were measured by western blotting after 48 hours. RESULTS: FCC displayed a dose- and time-dependent inhibition of the SMMC-7721 cell proliferations in vitro. It also induced apoptosis with 45.4% and caused cell accumulation in G0/G1 phase with 21.5%. PCNA and Bcl-2 expression was significantly suppressed by FCC in a dose-dependent manner (P < 0.05), while Bax expression was increased. CONCLUSIONS: FCC could significantly inhibit HCC cell growth in vitro through cell cycle arrest and inducing apoptosis by suppressing PCNA expression and modulating the Bax/Bcl-2 ratio

Mosaic loss of chromosome Y, tobacco smoking, and risk of age-related lung diseases: insights from two prospective cohorts

Background Little is known about the underlying relationship between mosaic loss of chromosome Y (mLOY), the most common chromosomal alterations in older men, and the risk of age-related lung diseases. Methods We included 217 780 participants from the UK Biobank (UKB) and 42 859 participants from the China Kadoorie Biobank. The mLOY events were detected using the Mosaic Chromosomal Alterations (MoChA) pipeline. Outcomes included all lung diseases, COPD, lung cancer and idiopathic pulmonary fibrosis (IPF). Cox proportional hazard models were fitted to estimate the hazard ratios and 95% confidence intervals of mLOY with lung diseases in both cohorts. The combined hazard ratios were derived from meta-analysis. Results Results from the two cohorts showed that expanded mLOY was associated with increased risks of all lung diseases (HR 1.19 (95% CI 1.04–1.36)), COPD (HR 1.20 (95% CI 1.13–1.28)), lung cancer (HR 1.34 (95% CI 1.21–1.48)) and IPF (HR 1.34 (95% CI 1.16–1.56) in the UKB). There was evidence of positive interactions between mLOY and smoking behaviour (relative excess risk due to interaction (97.5% CI) >0). Additionally, we observed that current smokers with expanded mLOY had the highest risk of incident lung diseases in both cohorts. Conclusions mLOY may be a novel predictor for age-related lung diseases. For current smokers carrying mLOY, adopting quitting smoking behaviour may contribute to substantially reducing their risk of incident lung diseases

Epidemic characteristics and transmission risk prediction of brucellosis in Xi'an city, Northwest China

Human brucellosis (HB) has re-emerged in China since the mid-1990s, and exhibited an apparent geographic expansion shifted from the traditional livestock regions to the inland areas of China. It is often neglected in non-traditional epidemic areas, posing a serious threat to public health in big cities. We carried out a retrospective epidemiological study in Xi'an, the largest city in northwestern China. It utilizes long-term surveillance data on HB during 2008–2021 and investigation data during 2014–2021. A total of 1989 HB cases were reported in Xi'an, consisting of 505 local cases, i.e., those located in Xi'an and 1,484 non-local cases, i.e., those located in other cities. Significantly epidemiological heterogeneity was observed between them, mainly owing to differences in the gender, occupation, diagnostic delays, and reporting institutions. Serological investigations suggested that 59 people and 1,822 animals (sheep, cattle, and cows) tested positive for brucellosis from 2014 to 2021, with the annual average seroprevalence rates were 1.38 and 1.54%, respectively. The annual animal seroprevalence rate was positively correlated with the annual incidence of non-local HB cases. Multivariate boosted regression tree models revealed that gross domestic product, population density, length of township roads, number of farms, and nighttime lights substantially contributed to the spatial distribution of local HB. Approximately 7.84 million people inhabited the potential infection risk zones in Xi'an. Our study highlights the reemergence of HB in non-epidemic areas and provides a baseline for large and medium-sized cities to identify regions, where prevention and control efforts should be prioritized in the future

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Macro–Micro Correlation Mechanism Between Structural Potential and Mechanical Strength in Collapsible Loess

The collapsibility of loess in the northwest region poses a significant threat to infrastructure stability. Current research predominantly separates macroscopic mechanical behaviour from mesoscopic structural characteristics, lacking a systematic methodology to quantify their interdependence. This study integrates consolidation tests, laser particle size analysis, mercury intrusion porosimetry (MIP) tests, and fractal theory to propose a multi-scale evaluation framework for assessing the structural potential of collapsible loess in strength, with on-site verification conducted. This framework quantitatively links grain size, pore potential, and connection potential to the collapsibility of loess. The experimental results indicate that loess’s high compressibility and collapsibility are primarily governed by grain size and pore potential. In contrast, the connection potential of soluble salts mitigates structural instability through ionic bonding. Field verification demonstrates a strong correlation between the three structural potentials and the subsidence coefficient (R2 = 0.92, p < 0.01), validating the framework’s effectiveness in evaluating structural stability. A ternary evaluation system has been established based on fractal dimension, void ratio, and soluble salt content. These research findings provide predictive tools for managing collapse risks in loess-related projects, enabling fixed-point design of loess foundations and early warning of collapse risks

MicroRNA 483-3p suppresses the expression of DPC4/Smad4 in pancreatic cancer

AbstractBoth deregulation of tumor-suppressor genes and misexpression of microRNAs (miRNAs) have been implicated in the development of pancreatic cancer, but their relationship during this process remains less clear. Here, we report that the expression of miR-483-3p is strongly enhanced in pancreatic cancer tissues compared to side normal tissues using a miRNA-array differential analysis. Furthermore, DPC4/Smad4 is identified as a target of miR-483-3p and their expression levels are inversely correlated in human clinical specimens. Ectopic expression of miR-483-3p significantly represses DPC4/Smad4 protein levels in pancreatic cancer cell lines, and simultaneously promotes cell proliferation and colony formation in vitro. Our findings identify miR-483-3p as a potent regulator of DPC4/Smad4, which may provide a novel therapeutic strategy for the treatment of DPC4/Smad4-driven pancreatic cancer

Satellite-driven evidence of forest-induced temperature variability and its biophysical and biogeochemical pathways across latitudes

Forests significantly influence local temperature dynamics, although the specifics of their impacts and mechanisms exhibit global variability. This study investigates the cooling or warming effects of global forests from 2001 to 2021 using multi-satellite data. The results indicate that (1) boreal forests exhibit a significant warming effect of + 1.99 °C. Temperate forests exhibit nighttime warming but notable daytime cooling effect, resulting in a net daily cooling effect (−0.48 °C in the northern hemisphere, −0.91 °C in the southern hemisphere). The daily cooling effects peak in summer and gradually rise from spring to autumn, with winter exhibiting a warming inclination. Tropical forests consistently provide a cooling effect year-round (−2.11 °C). (2) Over the study period, tropical forests consistently revealed robust and stable cooling effects. Temperate forests displayed modest fluctuations in cooling effects, while the warming effect of boreal forest showed a slow trend upwards at a rate of + 0.03 °C per year. (3) The warming effect of boreal forests is primarily due to NEE (net ecosystem exchange) and ET pathways (indirect effect: +0.253 and + 0.392), while tropical forest cooling is driven by increased evapotranspiration (indirect effect: −0.938). As for temperate zones, annual cooling is primarily led by the NEE pathway (NH: −0.055 and SH: −0.415). (4) A robust annual coherence emerges between forests’ temperature regulation effects and ΔNEE, ΔET, and Δalbedo, where augmented ET and albedo significantly amplify cooling effects synchronously. The decrease in NEE exhibits a positive but non-synchronous impact on cooling at the local scale, while showing a strong and synchronous relationship with ΔLST at the global scale. These findings highlight the crucial role of forests in local temperature regulation, necessitating targeted management strategies