Pan African strategy for the progressive control of peste des petits ruminants (Pan African ppr strategy)

Peste des petits ruminants (PPR) is a major constraint to the livelihoods and food security of small scale farmers. The epidemiology and biology of PPR virus has much in common with rinderpest virus (RP), an agent that has been globally eradicated. This document presents a strategy for the progressive control of PPR that builds upon the lessons learnt from rinderpest eradication. Progressive control relies upon a modular approach that consists of a series of self-sufficient phases each with its own set of sustainable results. Key intermediate results will be proven business models for sustainable PPR control service delivery and enhanced capacity of animal health institutions to target control services to critical control points. The program will foster an adaptive management approach that integrates learning approaches to drive animal health institutional innovation. The coordinated drive towards long term animal health goals will add value to on-going investments in infectious disease control

Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

2D and 3D cryo-electron microscopy, together with adsorption kinetics assays of ϕCb13 and ϕCbK phage-infected Caulobacter crescentus, provides insight into the mechanisms of infection. ϕCb13 and ϕCbK actively interact with the flagellum and subsequently attach to receptors on the cell pole. We present evidence that the first interaction of the phage with the bacterial flagellum takes place through a filament on the phage head. This contact with the flagellum facilitates concentration of phage particles around the receptor (i.e., the pilus portals) on the bacterial cell surface, thereby increasing the likelihood of infection. Phage head filaments have not been well characterized and their function is described here. Phage head filaments may systematically underlie the initial interactions of phages with their hosts in other systems and possibly represent a widespread mechanism of efficient phage propagation

Synergistic antimicrobial interaction between honey and phage against Escherichia coli biofilms

CEB Annual Meeting 2017Chronic wounds that take months, years or may even never heal present a major biological and financial problem on both individual patients and the broader health system. Chronic wounds afford a hostile environment of damaged tissues that allow bacterial proliferation and further wound colonization. Wound colonization by bacterial biofilms is one of the main obstacles of chronic wounds healing. Biofilms are structured communities of bacterial cells enclosed in a self-produced polymeric matrix and adhered to an inert or living surface. Escherichia coli is among the most common colonizers of infected wounds and it is a prolific biofilm former. Living in biofilm communities, cells are protected, become more difficult to control and eradicate, and less susceptible to antibiotic therapy. Due to the vast increase of antibiotic resistant bacteria, there is a renewed interestin pre-antibiotic therapies. Years before the discovery of modern antibiotics, bacteriophages(phages) that are bacterial viruses, and beehive products such as honey were extensively used for their antimicrobial properties. Phages, are the natural bacterial enemies and have proven efficacy towards antibiotic-resistant bacteria, have self-replicating nature, do not interfere with the commensal flora and many studies acknowledge that phages can destroy, tovarying extent, mono and mixed biofilm populations. Honey, on the other hand, has a broad spectrum antibacterial activity against bacteria and its high viscosity provides a protective barrier against infections being suitable for skincare, promoting the wound healing, tissue regeneration and anti-inflammatory process. This work presents insights into the proceedings triggering E.coli biofilm control with phage, two Portuguese(PT) honeys and their combination, achieved through standard antimicrobial activity assays, zeta potential and flow cytometry studies and further visual insights sought by SEM and TEM microscopy.This study was supported by FCT under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorteoperation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersio

Genetic and physical map of broad host range cosmid pRG930cm

We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/ streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteri

Bacteriophages with the Ability to Degrade Uropathogenic Escherichia coli Biofilms

Abstract: Escherichia coli-associated urinary tract infections (UTIs) are among the most common bacterial infections in humans. UTIs are usually managed with antibiotic therapy, but over the years, antibiotic-resistant strains of uropathogenic E. coli (UPEC) have emerged. The formation of biofilms further complicates the treatment of these infections by making them resistant to killing by the host immune system as well as by antibiotics. This OPEN ACCESS Viruses 2012, 4 472 has encouraged research into therapy using bacteriophages (phages) as a supplement or substitute for antibiotics. In this study we characterized 253 UPEC in terms of their biofilm-forming capabilities, serotype, and antimicrobial resistance. Three phages were then isolated (vB_EcoP_ACG-C91, vB_EcoM_ACG-C40 and vB_EcoS_ACG-M12) which were able to lyse 80.5% of a subset (42) of the UPEC strains able to form biofilms. Correlation was established between phage sensitivity and specific serotypes of the UPEC strains. The phages' genome sequences were determined and resulted in classification of vB_EcoP_ACG-C91 as a SP6likevirus, vB_EcoM_ACG-C40 as a T4likevirus and vB_EcoS_ACG-M12 as T1likevirus. We assessed the ability of the three phages to eradicate the established biofilm of one of the UPEC strains used in the study. All phages significantly reduced the biofilm within 2-12 h of incubation

Bacteriophages with the Ability to Degrade Uropathogenic Escherichia Coli Biofilms

Escherichia coli-associated urinary tract infections (UTIs) are among the most common bacterial infections in humans. UTIs are usually managed with antibiotic therapy, but over the years, antibiotic-resistant strains of uropathogenic E. coli (UPEC) have emerged. The formation of biofilms further complicates the treatment of these infections by making them resistant to killing by the host immune system as well as by antibiotics. This has encouraged research into therapy using bacteriophages (phages) as a supplement or substitute for antibiotics. In this study we characterized 253 UPEC in terms of their biofilm-forming capabilities, serotype, and antimicrobial resistance. Three phages were then isolated (vB_EcoP_ACG-C91, vB_EcoM_ACG-C40 and vB_EcoS_ACG-M12) which were able to lyse 80.5% of a subset (42) of the UPEC strains able to form biofilms. Correlation was established between phage sensitivity and specific serotypes of the UPEC strains. The phages’ genome sequences were determined and resulted in classification of vB_EcoP_ACG-C91 as a SP6likevirus, vB_EcoM_ACG-C40 as a T4likevirus and vB_EcoS_ACG-M12 as T1likevirus. We assessed the ability of the three phages to eradicate the established biofilm of one of the UPEC strains used in the study. All phages significantly reduced the biofilm within 2–12 h of incubation

Pseudomonas aeruginosa lytic bacteriophage oKMV receptor analysis

status: publishe