657 research outputs found
Linked sucrose synthase genes in group-7 chromosomes in hexaploid wheat (Triticum aestivum L.)
A cDNA library from developing wheat endosperm was screened for sucrose-synthase clones using a maize cDNA probe corresponding to the Sh1 locus under non-stringent conditions. Five positive clones were isolated and initially classified into two types on the basis of their relative ability to hybridize with the probe and of their partial restriction maps. Determination of the nucleotide sequences indicated homology between the two types of wheat clones, with type 1 showing higher homology to the maize Sh1 locus than to type-2 sequences. The inserts cloned in plasmids pST8 (type 1) and pST3 (type 2) were used as probes to determine the chromosomal locations of the two types of genes. DNAs from compensated nulli-tetrasomic and ditelosomic lines of wheat cultivar Chinese Spring were cleaved with EcoRI and analysed in Southern blots. DNA segments of the two types were thus identified in the short arms of chromosomes 7A, 7D, and, possibly, 7B. The two types of linked loci have been designated Ss1 and Ss2, respectivel
Forensic Technique for Detection of Image Forgery
Todays digital image plays an important role in all areas such as baking, communication, business etc. Due to the availability of manipulation software it is very easy to manipulate the original image. The contents in an original image can be copy-paste to hide some information or to create tampering. The new area introduces to detect the forgery is an image forensic. In this paper proposes the new image forensic technique to detect the presence of forgery in the compressed images and in other format images. The proposed method is based on the no subsampled contoured transform (NSCT). The proposed method is made up of three parts as preprocessing, nsct transform and forgery detection. The proposed forensic method is flexible, multiscale, multidirectional, and image decomposition is shift invariant that can be efficiently implemented via the à trous algorithm. The proposed a design framework based on the mapping approach. This method allows for a fast implementation based on a lifting or ladder structure. The proposed method ensures that the frame elements are regular, symmetric, and the frame is close to a tight one. The NSCT compares with and dct method in this paper
Differential expression of two types of sucrose synthase-encoding genes in wheat in response to anaerobiosis, cold shock and light
The expression of two types of sucrose synthase-encoding genes, Ss1 and Ss2, in hexaploid wheat (Triticum aestivum, L.), has been investigated using type-specific probes, corresponding to the 250–270 bp C-terminal portions of the respective cDNA clones. Both types of genes are highly expressed in developing endosperm, where the expression of the Ss2 type slightly precedes in time that of the Ss1 type. Expression of Ss genes is lower in etiolated leaves and in roots than in endosperm. In the first two tissues, the Ss1 mRNA is much more abundant than the Ss2 mRNA, and the Ss1 mRNA level sharply increases in response to anerobiosis and to cold shock (6°C), while the level of Ss2 mRNA is not significantly affected. Upon illumination of etiolated leaves, the Ss1 level mRNA decreases significantly and the Ss2 mRNA level increases
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A name has the power to evoke. It can stir our hopes and inspire our efforts. At Furman, named gifts also symbolize academic prestige, athletic excellence, the embrace of community and oppostunities that transform lives
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