The t6A Modification Acts as a Positive Determinant for the Anticodon Nuclease Prrc, and is Distinctively Nonessential in Streptococcus mutans

Endoribonuclease toxins (ribotoxins) are produced by bacteria and fungi to respond to stress, eliminate non-self competitor species, or interdict virus infection. PrrC is a bacterial ribotoxin that targets and cleaves tRNALys UUU in the anticodon loop. In vitro studies suggested that the post-transcriptional modification threonylcarbamoyl adenosine (t6A) is required for PrrC activity but this prediction had never been validated in vivo. Here, by using t6A-deficient yeast derivatives, it is shown that t6A is a positive determinant for PrrC proteins from various bacterial species. Streptococcus mutans is one of the few bacteria where the t6A synthesis gene tsaE (brpB) is dispensable and its genome encodes a PrrC toxin. We had previously shown using an HPLC-based assay that the S. mutans tsaE mutant was devoid of t6A. However, we describe here a novel and a more sensitive hybridization-based t6A detection method (compared to HPLC) that showed t6A was still present in the S. mutans ΔtsaE, albeit at greatly reduced levels (93% reduced compared to WT). Moreover, mutants in two other S. mutans t6A synthesis genes (tsaB and tsaC) were shown to be totally devoid of the modification thus confirming its dispensability in this organism. Furthermore, analysis of t6A modification ratios and of t6A synthesis genes mRNAs levels in S. mutans suggest they may be regulated by growth phase

Die SAGAT-Methode: Welche Informationen muss der Towerlotse erfassen?

<div><p>DNA methylation regulates gene expression in many organisms. In eukaryotes, DNA methylation is associated with gene repression, while it exerts both activating and repressive effects in the Proteobacteria through largely locus-specific mechanisms. Here, we identify a critical DNA methyltransferase in <i>M. tuberculosis</i>, which we term MamA. MamA creates N⁶-methyladenine in a six base pair recognition sequence present in approximately 2,000 copies on each strand of the genome. Loss of MamA reduces the expression of a number of genes. Each has a MamA site located at a conserved position relative to the sigma factor −10 binding site and transcriptional start site, suggesting that MamA modulates their expression through a shared, not locus-specific, mechanism. While strains lacking MamA grow normally <i>in vitro</i>, they are attenuated in hypoxic conditions, suggesting that methylation promotes survival in discrete host microenvironments. Interestingly, we demonstrate strikingly different patterns of DNA methyltransferase activity in different lineages of <i>M. tuberculosis</i>, which have been associated with preferences for distinct host environments and different disease courses in humans. Thus, MamA is the major functional adenine methyltransferase in <i>M. tuberculosis</i> strains of the Euro-American lineage while strains of the Beijing lineage harbor a point mutation that largely inactivates MamA but possess a second functional DNA methyltransferase. Our results indicate that MamA influences gene expression in <i>M. tuberculosis</i> and plays an important but strain-specific role in fitness during hypoxia.</p></div

MamA affects viability in hypoxic conditions.

<p>The indicated strains of H37Rv were normalized to a calculated density of 3×10⁶ CFU/ml and sealed in bottles containing equal volumes of culture and headspace. (A) Two bottles per strain were opened at the indicated timepoints and CFU/ml determined by plating. Error bars denote standard deviation. The negative slopes of the time points between day 14 and day 35 differ significantly between <i>ΔmamA</i> and the other two strains (P<0.05, linear regression of log₁₀-transformed values according to the method in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat.1003419-Zar1" target="_blank">[96]</a>). (B) After 28 days, samples of culture were treated with fluorescein diacetate and visualized by microscopy. Only live cells containing active intracellular esterases cleave fluorescein diacetate to produce fluorescent fluorescein. Scale bar = 10 µm. (C) Quantification of percent fluorescent bacteria in three-four fields at day 28. Error bars denote 95% confidence intervals. P<0.05 for all inter-strain comparisons (Fisher's exact test).</p

Several genes have lower expression levels in a <i>ΔmamA</i> strain.

<p>Expression of each gene was determined by quantitative PCR in the indicated H37Rv-derived strains and is displayed as a relative value compared to expression of the housekeeping gene <i>sigA</i> in the same strain. Values shown are the mean of three technical replicates. Error bars denote standard deviation. (*) denotes P<0.05 compared to the wildtype and complemented strains (ANOVA with Tukey's post test). The wildtype and complemented strains were not significantly different from each other for any of the genes tested. The experiment was performed using RNA from different cultures than those used to prepare RNA for microarrays.</p

Quantitation of total N⁶-MdA content in <i>M. tuberculosis</i>.

<p>Genomic DNA from the indicated strains was digested to individual nucleosides and methylation content determined by liquid chromatography-coupled tandem mass spectrometry. Results are expressed as the amount of N⁶-MdA per nucleotide (left axis) and percentage of adenines that are methylated in each genome (right axis). Each represents at least three biological replicates. Outliers were removed using Grubbs criteria and error bars represent ± standard deviation. (A) Analysis of the contribution of wildtype and mutant forms of MamA to total adenine methylation levels in strain H37Rv. (B) Analysis of the contributions of MamA and HsdM to total adenine methylation levels in Euro-American (H37Rv) and Beijing (HN878) strain backgrounds.</p

Deletion of <i>mamA</i> does not grossly affect growth rate or fitness of <i>M. tuberculosis</i> during mouse infection.

<p>(A) The indicated H37Rv-derived strains were normalized at a calculated optical density of 0.01 in Sauton's media and monitored by optical density on the days indicated. Points indicate the mean of triplicate cultures and error bars denote standard deviation. Similar results were obtained in 7H9 medium and by plating for CFU. (B) Mice were infected by the aerosol route with approximately 10,000 CFU of a mixture of unmarked wildtype H37Rv and one of three isogenic <i>mamA</i> mutants marked with kanamycin resistance. Groups of four mice per condition were sacrificed at the indicated time points and the lung burden of total and marked bacilli was determined. The mean proportion of marked bacteria is indicated. Error bars denote standard deviation.</p

Genes with expression differences of 1.5-fold or greater in Δ<i>mamA</i> or complemented strains compared to the wildtype parent (H37Rv) in aerobic growth conditions.

a<p>ANOVA.</p>b<p>Method of Benjamini and Hochberg.</p>c<p>Distance from start of ORF to nearest upstream MamA site.</p>d<p>Distance from start of first ORF in operon (Rv3083) to nearest upstream MamA site.</p>e<p>Distances in wildtype/Δ<i>mamA</i> and complemented strains, respectively; complementation vector integrates in this region.</p>f<p>Complementation vector integrates in this gene.</p

Sequence trace comparison identifies the target base and minimal recognition sequence of MamA.

<p>Plasmids containing putative MamA-recognition motifs were propagated in the indicated bacterial strains, isolated and sequenced. Sequence traces shown are representative of at least 2–3 biological replicates. (A) The 10 base pair sequence shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat-1003419-g001" target="_blank">Figure 1E</a> supports methylation of one adenine on each strand in wildtype H37Rv, as evidenced by increased thymine peak areas relative to the identical sequence context in <i>E. coli</i> and methylation-deficient strains of <i>M. tuberculosis</i>. See <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat.1003419.s002" target="_blank">Figure S2</a> for quantification of peak areas. (B) Schematic depiction of the positions of N⁶-methyladenine residues. (C) A six base pair core sequence is sufficient to direct MamA-mediated methylation (bold in panel B). See <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003419#ppat.1003419.s008" target="_blank">Table S1</a> for a complete list of tested sequences. (D) Positions of MamA recognition sequences are shown schematically on the 4.4 Mb <i>M. tuberculosis</i> genome.</p

MamA is a DNA methyltransferase that protects <i>lppC</i> from endonucleolytic cleavage.

<p>(A) Southern blotting strategy to assess the status of a PvuII site near the 3′ end of <i>lppC</i>. Genomic DNA was digested with PvuII and analyzed by Southern blot with a probe hybridizing as shown. Fully cleaved DNA generates a 1.8 kb product, while protected DNA produces a 2.1 kb product. (B) DNA from the vaccine strain <i>M. bovis</i> BCG and from <i>M. tuberculosis</i> strains of the Euro-American lineage (Erdmann and CDC1551) is partially protected from PvuII cleavage while DNA from a Beijing lineage strain (HN878) is not. (C) Genetic deletion of <i>mamA</i> abrogates protection of <i>lppC</i>, while deletion of <i>hsdM</i> does not affect protection. (D) Protection is restored by complementation of a <i>ΔmamA</i> strain with an ectopic copy of <i>mamA</i>, but not by empty vector or <i>mamA^E270A</i>. (E) Sequence context of the assayed PvuII site. Underlined bases are predicted to block PvuII if methylated.</p