Study of High Efficiency Flow Regulation of VIGV in Centrifugal Compressor

Variable inlet guide vane (VIGV) is used to control the mass flow and generate prewhirl in centrifugal compressors. Due to the tip clearance of the guide vanes and the defect of the traditional guide vane profiles, the mass flow regulation of VIGV is limited, resulting in a large waste of compressed gas. Two kinds of inlet flow channels were proposed to eliminate the influence of tip clearance. These structures were numerically investigated at different setting angles. The results show that the improved channels not only expand the range of mass flow regulation, but also reduce the power and increase the efficiency of the compressor. Ten kinds of guide vane profiles, including different thickness distribution, camber line profile, were selected to compare with the original one and with each other. In the premise of ensuring the performance of compressor, the best guide vane profile was selected. The results show that reducing the guide vane thickness, increasing the guide vane camber angle, and increasing the distance between the maximum camber position and the leading edge of guide vane can help expand the range of mass flow regulation. The achievement of this research can effectively improve the flow regulation ability of VIGV and the performance of compressor

A polyketide-based biosynthetic platform for diols, amino alcohols and hydroxy acids

Medium- and branched-chain diols and amino alcohols are important industrial solvents, polymer building blocks, cosmetics and pharmaceutical ingredients, yet biosynthetically challenging to produce. Here we present an approach that uses a modular polyketide synthase (PKS) platform for the efficient production of these compounds. This platform takes advantage of a versatile loading module from the rimocidin PKS and nicotinamide adenine dinucleotide phosphate-dependent terminal thioreductases. Reduction of the terminal aldehyde with alcohol dehydrogenases enables the production of diols, oxidation enables the production of hydroxy acids and specific transaminases allow the production of various amino alcohols. Furthermore, replacement of the malonyl-coenzyme A-specific acyltransferase in the extension module with methyl- or ethylmalonyl-coenzyme A-specific acyltransferase enables the production of branched-chain diols, amino alcohols and carboxylic acids in high titres. Use of our PKS platform in Streptomyces albus demonstrated the high tunability and efficiency of the platform

Organic-Inorganic Perovskite Light-Emitting Electrochemical Cells with a Large Capacitance

While perovskite light-emitting diodes typically made with high work function anodes and low work function cathodes have recently gained intense interests. Perovskite light-emitting devices with two high work function electrodes with interesting features are demonstrated here. Firstly, electroluminescence can be easily obtained from both forward and reverse biases. Secondly, the results of impedance spectroscopy indicate that the ionic conductivity in the iodide perovskite (CH3 NH3PbI3) is large with a value of approximate to 10(-8) S cm(-1). Thirdly, the shift of the emission spectrum in the mixed halide perovskite (CH3NH3PbI3-Br-x(x)) light-emitting devices indicates that I(-)ions are mobile in the perovskites. Fourthly, this work shows that the accumulated ions at the interfaces result in a large capacitance (approximate to 100 mu F cm(-2)). The above results conclusively prove that the organic-inorganic halide perovskites are solid electrolytes with mixed ionic and electronic conductivity and the light-emitting device is a light-emitting electrochemical cell. The work also suggests that the organic-inorganic halide perovskites are potential energy-storage materials, which may be applicable in the field of solid-state supercapacitors and batteries.While perovskite light-emitting diodes typically made with high work function anodes and low work function cathodes have recently gained intense interests. Perovskite light-emitting devices with two high work function electrodes with interesting features are demonstrated here. Firstly, electroluminescence can be easily obtained from both forward and reverse biases. Secondly, the results of impedance spectroscopy indicate that the ionic conductivity in the iodide perovskite (CH3NH3PbI3) is large with a value of ≈10-8 S cm-1. Thirdly, the shift of the emission spectrum in the mixed halide perovskite (CH3NH3PbI3-xBrx) light-emitting devices indicates that I- ions are mobile in the perovskites. Fourthly, this work shows that the accumulated ions at the interfaces result in a large capacitance (≈100 μF cm-2). The above results conclusively prove that the organic-inorganic halide perovskites are solid electrolytes with mixed ionic and electronic conductivity and the light-emitting device is a light-emitting electrochemical cell. The work also suggests that the organic-inorganic halide perovskites are potential energy-storage materials, which may be applicable in the field of solid-state supercapacitors and batteries. Light-emitting electrochemical cells (LECs) of organic-inorganic perovskite (CH3NH3PbI3) with two high work function electrodes are demonstrated. Results indicate that CH3NH3PbI3 has an ionic conductivity of ≈10-8 S cm-1. The accumulated ions at the interfaces result in a large capacitance, which suggests a potential application in electrochemical energy-storage devices, such as solid-state supercapacitors and batteries

Improved CAR internalization and recycling through transmembrane domain optimization reduces CAR-T cytokine release and exhaustion

BackgroundAnti-CD19 chimeric antigen receptor T (CAR-T) cell therapy has proven effective for treating relapsed or refractory acute B cell leukemia. However, challenges such as cytokine release syndrome, T cell dysfunction, and exhaustion persist. Enhancing CAR-T cell efficacy through changing CAR internalization and recycling is a promising approach. The transmembrane domain is the easiest motif to optimize for modulating CAR internalization and recycling without introducing additional domains, and its impact on CAR internalization and recycling has not yet been thoroughly explored. In this study, we aim to enhance CAR-T cell function by focusing on the solely transmembrane domain design.MethodsUtilizing plasmid construction and lentivirus generation, we get two different transmembrane CAR-T cells [19CAR-T(1a) and 19CAR-T(8α)]. Through co-culture with tumor cells, we evaluate CAR dynamic change, activation levels, exhaustion markers, mitochondrial function, and differentiation in both CAR-T cells. Furthermore, immunofluorescence microscopy analysis is performed to reveal the localization of internalized CAR molecules. RNA sequencing is used to detect the transcriptome of activated CAR-T cells. Finally, a mouse study is utilized to verify the anti-tumor efficacy of 19CAR-T(1a) cells in vivo.ResultsOur findings demonstrate that 19CAR-T(1a) has lower surface CAR expression, faster internalization, and a higher recycling rate compared to 19CAR-T(8α). Internalized 19CAR(1a) co-localizes more with early and recycling endosomes, and less with lysosomes than 19CAR(8α). These features result in lower activation levels, less cytokine release, and reduced exhaustion markers in 19CAR-T(1a). Furthermore, CAR-T cells with CD1a transmembrane domain also exhibit a superior anti-tumor ability and reduced exhaustion in vivo.ConclusionOverall, we demonstrate that the transmembrane domain plays a critical role in CAR-T cell function. An optimized transmembrane domain can alleviate cytokine release syndrome and reduce CAR-T cell exhaustion, providing a direction for CAR design to enhance CAR-T cell function