Changes on fecal microbiota in rats exposed to permethrin during postnatal development

Alteration of the gut microbiota through diet and environmental contaminants may disturb the mammalian digestive system, leading to various diseases. Because most exposure to environmentally pyrethroid pesticides such as permethrin (PERM) occurs through the diet, the commensal gut microbiota is likely to be exposed to PERM. The study aimed at evaluating the effect of low-dose exposure to PERM in early life on the composition of fecal microbiota in rats. Over a 4-month follow-up period, fecal microbiota and short-chain fatty acids were measured in order to identify possible differences between PERM-treated rats and controls. Further in vitro antimicrobial experiments were conducted to establish the antibacterial activity of PERM against different strains to obtain Minimal Inhibitory Concentrations. The main finding focused on the reduced abundance of Bacteroides-Prevotella-Porphyromonas species, increased Enterobacteriaceae and Lactobacillus in PERM-treated rats compared to controls. Changes of acetic and propionic acid levels were registered in PERM-treated group. From in vitro studies, PERM showed higher antibacterial activity against beneficial bacteria such as Bifidobacterium and Lactobacillus paracasei, while to inhibit potential pathogens as Staphylococcus aureus and Escherichia coli PERM concentration needed to be increased. In summary, exposure to PERM could affect the fecal microbiota and could be a crucial factor contributing to the development of diseases

Impact of Probiotic SYNBIO Administered by Vaginal Suppositories in Promoting Vaginal Health of Apparently Healthy Women

The purpose of this study was to investigate whether vaginal administration of probiotic Lactobacillus results in their colonization and persistence in the vagina and whether it promotes normalization and maintenance of pH and Nugent score. A single-arm, open-label controlled towards the baseline (pre–post) study including 35 apparently healthy women was conducted. Each woman was examined three times during the study. Women were instructed to receive daily for 7 days, the probiotic suppositories SYNBIO® gin (Lactobacillus rhamnosus IMC 501® and Lactobacillus paracasei IMC 502®). Vaginal swabs were collected during visit 1, 2, and 3 to determine the total lactobacilli count, the presence of the two administered bacteria, the measure of the pH, and the estimation of Nugent score. Evaluation of treatment tolerability was based on analysis of the type and occurrence of adverse events. The probiotic vaginal suppository was well tolerated and no side effects were reported. Intermediate Nugent score was registered in 40 % of women at visit 1 and these intermediate scores reverted to normal at day 7 (end of treatment) in 20 % of subjects. Administration of SYNBIO® gin contributed to a significant increase in the lactobacilli level at visit 2. Molecular typing revealed the presence of the two strains originating from SYNBIO® gin in 100 % of women at visit 2 and 34 % at visit 3. No significant changes were registered for pH between visits. The SYNBIO® gin product is safe for daily use in healthy women and it could be useful to restore and maintain a normal vaginal microbiota

Probiotics and red fruit extracts with bioactive properties for innovative dietary supplements for human health.

Alimenti funzionali e nutraceutici per la salute. A cura delle Piattaforme Tematiche di Ateneo su "Alimenti e Nutrizione" e "Salute Umana ed Animale

Biogenic amines as freshness index of meat wrapped in a new active packaging system formulated with essential oils of Rosmarinus officinalis

Biogenic amines (BAs) are considered as an important indicator of freshness and quality of food. In this work, a new active packaging (AP) system for meat that, incorporating essential oil of Rosmarinus officinalis at 4% (w/w), inhibits the increase of BAs and the bacteria involved into their production was developed. BAs were analyzed by a SPE-HPLC-DAD method during the storage time of meat (0–7 d, 4 C). Results showed that, in each monitored day, Biogenic Amine Index (BAI) expressed in mgkg1 is lower in meat wrapped in AP with respect to that packed in polycoupled packaging (PP) (from 19% to 62%). A strong correlation was found between the inhibition of increase of putrescine, cadaverine, histamine and their bacteria producers such as Enterobacteriaceae, Pseudomonas spp. and Brocothrix thermospacta. By exploiting antimicrobial and antioxidant action of essential oil of R. officinalis, the new APs contribute to increase the shelf life of fresh meat and to preserve its important nutrients

Patients with multiple sclerosis: a burden and cost of illness study

Background Multiple sclerosis (MS) is a chronic neuroinflammatory and neurodegenerative disease negatively impacting patients' physical, psychological and social well-being with a significant economic burden. Objectives The study estimates MS burden and cost of illness in Italy from a societal perspective in 2019. Methods Information on the impact of the disease on daily activities, symptoms, employment, resource utilization and the role of caregivers was collected through questionnaires completed by 944 patients and caregivers. Results were stratified according to both disease severity and payer. Mean costs and overall costs were extrapolated from the sample to the Italian MS population considering published distribution of severity. Results The study showed a great impact of the disease on daily and work activities increasing with the disability. The overwhelming burden of fatigue emerged. Mean annual costs were estimated at euro39,307/patient (euro29,676, euro43,464 and euro53,454 in mild, moderate and severe cases, respectively). Direct healthcare costs were the major component (euro21,069), followed by indirect costs (euro15,004). The overall cost of the disease in Italy was euro4.8 billion. The National Healthcare System (NHS) sustained most of the costs (80%), most notably direct healthcare costs, while patients paid almost all non-healthcare expenses. Conclusions This study confirmed that MS carries a substantial burden to patients and society, highlighting the need for awareness of this disease

Pilot-scale Production and Viability Analysis of Freeze-Dried Probiotic Bacteria Using Different Protective Agents

The functional food industry requires an improvement of probiotic strain stability during storage, especially when they are stored at room temperature. In this study, the viability of freeze-dried Lactobacillus rhamnosus IMC 501® and Lactobacillus paracasei IMC 502® using different protective agents (i.e., glycerine, mannitol, sorbitol, inulin, dextrin, Crystalean®) was determined and compared with semi skimmed milk (SSM) control. No significant differences were observed between the tested protectants and the control (SSM) during storage at refrigerated conditions. During storage at room temperature, only glycerine was found to stabilize viability better than other tested substances

Organosilicon phantom for photoacoustic imaging

Photoacoustic imaging is an emerging technique. Although commercially available photoacoustic imaging systems currently exist, the technology is still in its infancy. Therefore, the design of stable phantoms is essential to achieve semiquantitative evaluation of the performance of a photoacoustic system and can help optimize the properties of contrast agents. We designed and developed a polydimethylsiloxane (PDMS) phantom with exceptionally fine geometry; the phantom was tested using photoacoustic experiments loaded with the standard indocyanine green dye and compared to an agar phantom pattern through polyethylene glycol-gold nanorods. The linearity of the photoacoustic signal with the nanoparticle number was assessed. The signal-to-noise ratio and contrast were employed as image quality parameters, and enhancements of up to 50 and up to 300%, respectively, were measured with the PDMS phantom with respect to the agar one. A tissue-mimicking (TM)-PDMS was prepared by adding TiO2 and India ink; photoacoustic tests were performed in order to compare the signal generated by the TM-PDMS and the biological tissue. The PDMS phantom can become a particularly promising tool in the field of photoacoustics for the evaluation of the performance of a PA system and as a model of the structure of vascularized soft tissues. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE

The Manifesto of Italian Universities Network for Service-Learning

Il Manifesto intende offrire una piattaforma comune per docenti, Corsi di Laurea, Dipartimenti, Scuole o Atenei che intendano istituzionalizzare il service-learning all’interno del proprio curriculum. Il documento è stato elaborato dalla Rete delle UNiversità Italiane per il Service-Learning (UNiSL) e rappresenta un primo risultato raggiunto grazie al confronto sugli esiti delle ricerche condotte e sulle esperienze documentate in Italia e all’estero. Nel documento si propone la definizione italiana di service-learning in contesto universitario e un glossario con nove parole chiave, ognuna accompagnata da una spiegazione, con l’obiettivo di comprendere in modo esaustivo gli approcci, i modelli teorici e le prospettive che caratterizzano la Rete.The Manifesto serves as a unifying framework for academics, degree courses, departments, schools and universities seeking to institutionalise service-learning into their curriculum. It has been developed by the Italian Universities Network for Service-Learning (UNiSL). It represents the initial outcome of a comprehensive analysis of research findings and documented experiences from Italy and abroad. This document introduces the Italian definition of service-learning in higher education, accompanied by a glossary consisting of nine key terms, each followed by an explanatory description. Its primary objective is to comprehensively understand the approaches, theoretical models and perspectives that characterise the Network

VaIutazione delI'attività prebiotica dell'estratto di Ienticchie sul microbiota intestinale mediante modello di fermentazione in vitro.

Razionale dello studio. Lestratto di lenticchia d un prodotto di origine vegetale che puo essere d'aiuto nella prevenzione/cura di una serie di patologie. L'oggetto del presente studio in vitro è la verifica dell'effetto preblotico dell'estratto di lenticchie, ricco in soiasaponine, nel favorire l'equilibrio della microflora intestinale. Metodi. Lo studio è stato eseguito utilizzando un modello di fermentazione in vitro per testare l'attività prebiotica dell'estratto di lenticchie rispetto ad un controllo, su di una flora batterica intestinale umana standard. Risultati. I risultati hanno mostrato un'azione prebiotica dell'estratto di lenticchie con un aumento significativo del numero dei bifidobatteri. lnfatti nel test di controllo la concentrazione dei bifidobatteri rimane stabile (circa 10E4 log UFC/ml), mentre nei test effettuati con inulina e con l'estratto di lenticchie è stato osservato un significativo (p<0,05) incremento di 3 log del loro numero, evidenziando quindi proprietà bifidogeniche dell'estratto testato simili a quelle dell'inulina. Conclusioni. l'estratto di lenticchie, nella forma liofila, rappresenta un importante ingrediente per la realizzazione di nutraceutici allo scopo di svolgere un effetto benefico a livello intestinale e di prevenire e trattare disordini gastrointestinali. Questo composto è inoltre totalmente di derivazione naturale in quanto estratto esclusivamente da un legume edibile e rappresenta una reale innovazione nel campo degli integratori dato che attualmente non ci sono prodotti in commercio aventi una tale formulazione

Assessing the extent and timing of chemosensory impairments during COVID-19 pandemic

Chemosensory impairments have been established as a specific indicator of COVID-19. They affect most patients and may persist long past the resolution of respiratory symptoms, representing an unprecedented medical challenge. Since the SARS-CoV-2 pandemic started, we now know much more about smell, taste, and chemesthesis loss associated with COVID-19. However, the temporal dynamics and characteristics of recovery are still unknown. Here, capitalizing on data from the Global Consortium for Chemosensory Research (GCCR) crowdsourced survey, we assessed chemosensory abilities after the resolution of respiratory symptoms in participants diagnosed with COVID-19 during the first wave of the pandemic in Italy. This analysis led to the identification of two patterns of chemosensory recovery, partial and substantial, which were found to be associated with differential age, degrees of chemosensory loss, and regional patterns. Uncovering the self-reported phenomenology of recovery from smell, taste, and chemesthetic disorders is the first, yet essential step, to provide healthcare professionals with the tools to take purposeful and targeted action to address chemosensory disorders and their severe discomfort