Exogenous NG-hydroxy-l-arginine causes nitrite production in vascular smooth muscle cells in the absence of nitric oxide synthase activity

AbstractNitric oxide (NO) production from exogenous NG-hydroxy-l-arginine (OH-l-Arg) was investigated in rat aortic smooth muscle cells in culture by measuring nitrite accumulation in the culture medium. As well, the interaction between OH-l-Arg and l-arginine uptake via the y+ cationic amino acid transporter was studied. In cells without NO-synthase activity, OH-l-Arg (1–1000 μM) induced a dose-dependent nitrite production with a half-maximal effective concentration (EC50) of 18.0 ± 1.5 μM (n = 4–7). This nitrite accumulation was not inhibited by the NO-synthase inhibitor NG-nitro-l-arginine methyl ester, l-NAME (300 μM). In contrast, it was abolished by miconazole (100 μM), an inhibitor of cytochrome P450. Incubation of vascular smooth muscle cells with LPS (10 μgml) induced an l-name inhibited nitrite accumulation, but did not enhance the OH-l-Arg induced nitrite production. OH-l-Arg and other cationic amino acids, L-lysine and l-ornithine, competitively inhibited [3H]-l-arginine uptake m rat aortic smooth muscle cells, with inhibition constants of 195 ± 23 μM(n = 12), 260 ± 40 μM(n= 5) and 330 ± 10 μM(n = 5), respectively. These results show that OH-l-Arg is recognized by the cationic l-amino acid carrier present in vascular smooth muscle cells and can be oxidized to NO and nitrite in these cells in the absence of NO-synthase, probably by cytochrome P450 or by a reaction involving a cytochrome P450 byproduct

Authentic Leadership and Positive Psychological Capital: The Mediating Role of Trust at the Group Level of Analysis

This study investigates the relationship between authentic leadership, trust, positive psychological capital (PsyCap), and performance at the group level of analysis. Data were collected from a small Midwestern chain of retail clothing stores, a context in which the needs for both authentic leadership and a positive sales staff are integral to the firm’s performance. Constructs were aggregated to the store (group) level to test relationships between perceptions of authentic leadership, trust in management, positive psychological capital, and performance. Trust in management was found to mediate the relationship between PsyCap and performance and to partially mediate the relationship be¬tween authentic leadership and performance. Future discussions and implications are discussed

Recent Developments in the Design of Conventional Cryptographic Algorithms

This paper examines proposals for three cryptographic primitives: block ciphers, stream ciphers, and hash functions. It provides an overview of the design principles of a large number of recent proposals, which includes the global structure, the number of rounds, the way of introducing non-linearity and diffusion, and the key schedule. The software performance of about twenty primitives is compared based on highly optimized implementations for the Pentium. The goal of the paper is to provided a technical perspective on the wide variety of primitives that exist today