A pilot study to detect the effects of a green-lipped mussel (Perna canaliculus) nutraceutical on working farm dogs with musculoskeletal abnormalities using accelerometry.

AIMS: To obtain preliminary data on changes in gait from the use of a green-lipped mussel (Perna canaliculus) extract product in working farm dogs with musculoskeletal abnormalities using accelerometry. METHODS: New Zealand working farm dogs (n = 32) with signs of musculoskeletal abnormalities were enrolled in a double-blinded, placebo-controlled cross-over study. Each dog was allocated to one of six groups to receive three trial substances (180 mg full fat green-lipped mussel extract (GLME180); 220 mg full fat green-lipped mussel extract (GLME220); placebo) in one of the six possible different orders. Each trial substance was administered orally once a day for an 8-week period, with a 4-week washout in between each. Dogs wore a collar-mounted triaxial accelerometer for the study duration. Diet and activity were not controlled. Accelerations were recorded continuously and analysed (n = 27) in 10-second activity epochs partitioned into daytime and night-time periods. Analysis of activity during the daytime period was limited to epochs when dogs were gaiting faster than a walk. The median and IQR of activity were determined for the daytime and night-time. Additionally, the 75th and 90th percentiles of daytime activity for each 24-hour period were determined. Mixed effects linear regression models were constructed to determine if each trial substance altered the response variables. RESULTS: During the daytime, the 90th percentile was higher when dogs were given GLME220 compared with the placebo (β coefficient 2.6; 95% CI = 0.25-4.94; p = 0.03). Dogs that started the trial with the GLME products had a higher 90th percentile activity compared with dogs that began with the placebo (β coefficient 26.26; 95% CI = 0.45-52.06; p = 0.046). The 75th percentile for activity was not affected by the GLME product. The daytime IQR was larger when dogs were given the GLME180 product compared with the placebo (β coefficient 1.25; 95% CI = 0.12-2.37; p = 0.03). Night-time median activity and the IQR was greater in dogs that started the trial with the GLME products than in dogs that began with the placebo. The night-time IQR for activity was greater for GLME180 than for the placebo. CONCLUSIONS: Administration of a low dose of the GLME-containing product increased peak activity in working farm dogs with signs of musculoskeletal abnormalities and may improve their performance. CLINICAL RELEVANCE: Even mildly affected working farm dogs might benefit from support of their musculoskeletal abnormalities, and this particular GLME-based product shows promise as an adjunct to other management strategies.fals

The Relationship between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cells

Fenestrations are transcellular pores in endothelial cells that facilitate transfer of substrates between blood and the extravascular compartment. In order to understand the regulation and formation of fenestrations, the relationship between membrane rafts and fenestrations was investigated in liver sinusoidal endothelial cells where fenestrations are grouped into sieve plates. Three dimensional structured illumination microscopy, scanning electron microscopy, internal reflectance fluorescence microscopy and two-photon fluorescence microscopy were used to study liver sinusoidal endothelial cells isolated from mice. There was an inverse distribution between sieve plates and membrane rafts visualized by structured illumination microscopy and the fluorescent raft stain, Bodipy FL C5 ganglioside GM1. 7-ketocholesterol and/or cytochalasin D increased both fenestrations and lipid-disordered membrane, while Triton X-100 decreased both fenestrations and lipid-disordered membrane. The effects of cytochalasin D on fenestrations were abrogated by co-administration of Triton X-100, suggesting that actin disruption increases fenestrations by its effects on membrane rafts. Vascular endothelial growth factor (VEGF) depleted lipid-ordered membrane and increased fenestrations. The results are consistent with a sieve-raft interaction, where fenestrations form in non-raft lipid-disordered regions of endothelial cells once the membrane-stabilizing effects of actin cytoskeleton and membrane rafts are diminished.Full Tex

A risk assessment for the introduction of African swine fever into the Pacific Island countries

falseApia, Samo

A risk assessment for the introduction of African swine fever into Kiribati

falseApia, Samo

Owner-Reported Health Events in Australian Farm Working Dogs.

Working farm dogs are an integral part of livestock farming in Australia but, currently, no studies have investigated their overall health. This study aimed to identify the prevalent diseases and injuries among these dogs, offering a baseline dataset. A total of 125 farm dog owners were recruited at a public working dog event and interviewed about the health of their dogs in the preceding 12 months. Information was provided about 526 dogs. Most dogs were Kelpies, and not neutered. Nine percent of the dogs had been sold, 6% had died, and 1% had been retired. Of the 444 remaining dogs, 24% had a health event in the past 12 months, while 13% had a health event that affected their work. The injuries (11%) mostly involved the musculoskeletal system or skin, while the non-traumatic illnesses (13%) commonly included arthritis. Forty-five percent of the injured dogs and 26% of the ill ones received veterinary treatment. The results indicate similarities to previous studies into working farm dogs. Given their unique challenges, further research is needed to ensure optimal care for farm dogs in Australiafals

A risk assessment for the introduction of African swine fever into the Federated States of Micronesia

Subharat, S, Han, J, Cogger, N. "A risk assessment for the introduction of African swine fever into the Federated States of Micronesia" Apia, Samoa, "Food and Agriculture Organization of the United Nations", 19 pages, 10.4060/cc0421en. Licence: CC BY-NC-SA 3.0 IGO.falseApia, Samo

A risk assessment for the introduction of African swine fever into Tuvalu

Subharat, S., Hee Han, J. and Cogger, N. “A risk assessment for the introduction of African swine fever into Tuvalu.” Apia, Samoa. First Published in “Food and Agriculture Organization of the United Nations.” 19 pages https://doi.org/10.4060/cc0421en Licence: CC BY-NC-SA 3.0 IGO.falseApia, Samo

Use of a Collar-Mounted Triaxial Accelerometer to Predict Speed and Gait in Dogs

Accelerometry has been used to measure treatment efficacy in dogs with osteoarthritis, although interpretation is difficult. Simplification of the output into speed or gait categories could simplify interpretation. We aimed to determine whether collar-mounted accelerometry could estimate the speed and categorise dogs' gait on a treadmill. Eight Huntaway dogs were fitted with a triaxial accelerometer and then recorded using high-speed video on a treadmill at a slow and fast walk, trot, and canter. The accelerometer data (delta-G) was aligned with the video data and records of the treadmill speed and gait. Mixed linear and logistic regression models that included delta-G and a term accounting for the dogs' skeletal sizes were used to predict speed and gait, respectively, from the accelerometer signal. Gait could be categorised (pseudo-R2 = 0.87) into binary categories of walking and faster (trot or canter), but not into the separate faster gaits. The estimation of speed above 3 m/s was inaccurate, though it is not clear whether that inaccuracy was due to the sampling frequency of the particular device, or whether that is an inherent limitation of collar-mounted accelerometers in dogs. Thus, collar-mounted accelerometry can reliably categorise dogs' gaits into two categories, but finer gait descriptions or speed estimates require individual dog modelling and validation. Nonetheless, this accelerometry method could improve the use of accelerometry to detect treatment effects in osteoarthritis by allowing the selection of periods of activity that are most affected by treatment.fals