New bodipy dyads for two-photons fluorescence imaging in cells

Fluorescent probes are essential tools for studying biological systems. The last decade has witnessed particular interest in the development of two-photon excitable probes, due to their advantageous features in tissue imaging compared to the corresponding one-photon probes [1]. Recently, we have designed and synthetized an aminonaphthalimide–BODIPY derivative as energy transfer cassettes and were found to show very fast and efficient BODIPY fluorescence sensitization [2]. This was observed upon one- and two-photon excitation, which extends the application range of the investigated bichromophoric dyads in terms of accessible excitation wavelengths. In order to increase the two-photon absorption of the system aminonaphthalimide fluorophore was replace with a Prodan analog (BODIPY dyad 1), which presents found a variety of applications as probes and labels in biology [3]. The two-photon absorption cross-section of the dyads is significantly incremented by the presence of the 6-acetyl-2-naphthylamine donor group. The emission maximum of a BODIPY fluorophore can significantly be red-shifted in comparison to their precursors by conjugation with aromatic aldehydes. [4] We use a synthetic strategy to obtain BODIPY dyad 2 that incorporates an imidazole ring. This molecule can be used in biological media as a near-neutral pH indicator based on one- and two-photon excitable BODIPY acceptor.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Separability and aggregate shocks in the life-cycle model of consumption: evidence from Spain

The purpose of this paper is to test the life-cycle permanent income hypothesis using an unbalanced panel from the Spanish family expenditure survey. Our model accounts for aggregate shocks and non-separability in the Euler equation among consumption goods, contrary to most of the Literature in this area. Our results do not indicate excess sensitivity of consumption growth to income

Getting Back on Track: Effects of a Community College Program for Probationary Students

Rates of graduation and degree completion at community colleges remain distressingly low. This report evaluates two versions of a program designed to help probationary students at community college succeed in school. One version increased the average number of credits earned, the proportion of students who earned a grade point average of "C" or higher, and the proportion who moved off probation

Goals/questions/metrics method and SAP implementation projects

During the last years some researchers have studied the critical success factors (CSFs) in ERP implementations. However, until now, no one has studied how these CSFs should be put in practice to help organizations achieve success in ERP implementations. This technical research report attempts to define the usage of Goals/Questions/Metrics (GQM) approach in the definition of a measurement system for ERP implementation projects. GQM approach is a mechanism for defining and interpreting operational, measurable goals. Lately, because of its intuitive nature the approach has gained widespread appeal. We present a metrics overview and a description of GQM approach. Then we provide an example of GQM application for monitoring sustained management support in ERP implementations. Sustained management support is the most cited critical success factor in ERP implementation projects.Postprint (published version

Innovation and job creation and destruction : evidence from Spain

In this paper we examine the effect of innovation on job creation and job destruction in Spanish manufacturing. Our empirical analysis is based on firm-level longitudinal data from which we have information on employment and innovation activity. The estimation approach consists of a two-step procedure that takes into account the fact that firms endogenously choose positive, negative or zero growth in employment, in which the selection mechanism is an ordered probit. Our results point out the importance of innovation variables on employment growth: innovative firms create more jobs -and destroy fewer- than non-innovative, and the degree of technological effort has a strong positive effect on net employment creation

HABITS AND HETEROGENEITY IN DEMANDS: A PANEL DATA ANALYSIS

We examine demand behaviour for intertemporal dependencies, using Spanishpanel data. We present evidence that there is both state dependence and correlatedheterogeneity in demand behaviour. Our specific findings are that food outside thehome, alcohol and tobacco are habit forming whereas clothing and small durablesexhibit durability. We conclude that demand analyses using cross-section data thatignore these effects may be seriously biased. On the other hand, the degree ofintertemporal dependence is not sufficiently strong to make composite `consumption'significantly habit forming, as has been suggested in some recent analyses.Habits, State dependence, correlated heterogeneity.

Two-Photon Fluorescence As Tool For Imaging In Cells

Fluorescent probes are essential tools for studying biological systems. The last decade has witnessed particular interest in the development of two-photon excitable probes, due to their advantageous features in tissue imaging compared to one-photon probes [1]. Recently, we have designed and synthesized an aminonaphthalimide–BODIPY derivative as energy transfer cassette which was found to show very fast and efficient BODIPY fluorescence sensitization [2]. This was observed upon one- and two-photon excitation, which extends the application range of the investigated bichromophoric dyads in terms of accessible excitation wavelengths. In order to increase the two-photon absorption of the system the aminonaphthalimide fluorophore was replaced with a Prodan analogue, which presents a variety of applications as probes and labels in biology [3]. The two-photon absorption cross-section of the dyads is significantly incremented by the presence of the 6-acetyl-2-naphthylamine donor group. We also explore in this communication the use of new fluorophores based on four-coordinate organoboron N,C-chelates containing an arylisoquinoline skeleton in confocal fluorescence microscopy that show significant two-photon absorption cross sections and allow the use of excitation wavelengths in the near-infrared region [4].References [1] H. M. Kim, B. R. Cho, Chem. Rev. 2015, 5014–5055. [2] D. Collado, P. Remón, Y. Vida, F. Nájera, P. Sen, U. Pischel, E. Perez-Inestrosa, Chem. Asian J. 2014, 9, 797–804. [3] O. A. Kucherak, P. Didier, Y. Mely, A. S. Klymchenko, J. Phys. Chem. Lett. 2010, 1, 616–620. [4] V. F. Pais, M. M. Alcaide, R. López-Rodriguez, D. Collado, F. Nájera, E. Perez-Inestrosa, E. Álvarez, J. M. Lassaletta, R. Fernández, A. Ros, U. Pischel, Chem. Eur. J. 2015, 21, 15369–15376.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Organizational and national issues of an ERP implementation in a Portuguese company

This technical research report describes a case of an Enterprise Resource Planning (ERP) implementation in a Portuguese SME. We focused on the identification of organizational factors that affect the ERP implementation project. We also analyzed the ERP implementation project from a national cultural perspective using Geert Hofstede's dimensions. These dimensions were used to explain some of the attitudes and behaviours during the ERP implementation project. The findings suggest that some of the problems in ERP implementation projects are not of technological nature but may be attributed to organizational factors while some issues related to national culture.Postprint (published version

INNOVATION AND JOB CREATION AND DESTRUCTION: EVIDENCE FROM SPAIN

In this paper we examine the effect of innovation on job creation and job destruction in Spanish manufacturing. Our empirical analysis is based on firm-level longitudinal data from which we have information on employment and innovation activity. The estimation approach consists of a two-step procedure that takes into account the fact that firms endogenously choose positive, negative or zero growth in employment, in which the selection mechanism is an ordered probit. Our results point out the importance of innovation variables on employment growth: innovative firms create more jobs –and destroy fewer– than non-innovative, and the degree of technological effort has a strong positive effect on net employment creation.