Up-regulation of CNDP2 facilitates the proliferation of colon cancer

BACKGROUND: Cytosolic nonspecific dipetidase (CN2) belongs to the family of M20 metallopeptidases. It was stated in previous articles that higher expression levels of CN2 were observed in renal cell carcinoma and breast cancer. Our study explored the correlation between CN2 and colon carcinogenesis. METHODS: We analysed the relationship between 183 patients clinicopathological characteristics and its CN2 expression. To detect the levels of CN2 in colon cancer cell lines and colon cancer tissues by western blot. To verify cell proliferation in colon cancer cells with knockdown of CNDP2 and explore the causes of these phenomena. RESULTS: The expression levels of CN2 in clinical colon tumors and colon cancer cell lines were significantly higher than that in normal colon mucosa and colon cell lines. The difference in CN2 levels was associated with tumor location (right- and left-sided colon cancer), but there was no significant association with age, gender, tumor size, tumor grade, tumor stage or serum carcinoembryonic antigen (CEA). Knockdown of CNDP2 inhibited cell proliferation, blocked cell cycle progression and retarded carcinogenesis in an animal model. The signaling pathway through which knockdown of CNDP2 inhibited cell proliferation and tumorigenesis involved in EGFR, cyclin B1 and cyclin E. CONCLUSIONS: Knockdown of CNDP2 can inhibit the proliferation of colon cancer in vitro and retarded carcinogenesis in vivo

Comparative analysis of chloroplast genome between wild and cultivated<i>Rehmannia glutinosa Libosch</i>

ABSTRACTRehmanniaeradix, as a tonic Chinese medicine, has a good therapeutic effect on cardiovascular and cerebrovascular diseases and central nervous system diseases, and has a good development prospect in the international market.R.glutinosaLibosch has a large number of source varieties, and the wild and cultivated varieties are different types ofR.glutinosaLibosch. There has been a lot of controversy in the formulation of scientific names for wild and cultivated types ofR.glutinosaLibosch. In order to learn more about the genetic information of wildR.glutinosaLibosch and explore the phylogenetic relationship between wildR.glutinosaLibosch and cultivatedR.glutinosaLibosch. In the current study, we constructed and annotated a complete circular chloroplast genome of wildR.glutinosaLibosch. The chloroplast genome of wildR.glutinosa Libosch. is 153,678 bp in length, including two inverted repeat (IR) regions of 25,759 bp, separated by a large single copy (LSC) region of 84,544 bp and a small single copy (SSC) region of 17,616 bp. The GC content of whole chloroplast genome is 37.9%. The genome contains 133 different genes, including 88 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Among them, 23 genes contained introns, 50 SSR loci and 49 long repeats were found by Microsatellite scan, and codon use showed obvious A, T base preference. Nine variation regions were screened by mVISTA analysis and combined with DnaSP analysis, and psaJ-rpl33 was finally identified as a region with high variation, which could be used as a candidate DNA barcode for later interspecific identification ofR.glutinosaLibosch.. Neighbor-joining method phylogenomic analysis showed that wildR.glutinosa Libosch. formed a monophyletic group, and was sister to other groups ofR.glutinosa Libosch.The results of this study revealed the chloroplast genome information of wildR.glutinosa Liboschin detail, and found the genetic information difference between wild and cultivatedR.glutinosa Libosch. which laid the foundation for the variety identification, genetic engineering, biosynthesis and other studies.</jats:p