Der Wahrsager

DER WAHRSAGER Der Wahrsager / Consentius, Ernst (Public Domain) ( - ) Handschriftliche Notiz ( - ) Title page ( - ) Preface ( - ) Verbot des Wahrsager ( - ) La Mettrie ([36]) Lessings Kritik an Wahrsager ([53]) Imprint ( - ) ColorChart ( -

Combining EPR spectroscopy and X ray crystallography to elucidate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals

Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling is used to investigate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals. Within a single crystal, the oriented ensemble of spin bearing moieties results in a strong angle dependence of the EPR spectra. A quantitative description of the EPR spectra requires the determination of the unit cell orientation with respect to the sample tube and the orientation of the spin bearing moieties within the crystal lattice. Angle dependent EPR spectra were analyzed by line shape simulations using the stochastic Liouville equation approach developed by Freed and co-workers and an effective Hamiltonian approach. The gain in spectral information obtained from the EPR spectra of single crystalline samples taken at different frequencies, namely the X-band and Q-band, allows us to discriminate between motional models describing the spectra of isotropic solutions similarly well. In addition, it is shown that the angle dependent single crystal spectra allow us to identify two spin label rotamers with very similar side chain dynamics. These results demonstrate the utility of single crystal EPR spectroscopy in combination with spectral line shape simulation techniques to extract valuable dynamic information not readily available from the analysis of isotropic systems. In addition, it will be shown that the loss of electron density in high resolution diffraction experiments at room temperature does not allow us to conclude that there is significant structural disorder in the system

Internal dynamics of the 3-Pyrroline-N-Oxide ring in spin-labeled proteins

Site-directed spin labeling is a versatile tool to study structure as well as dynamics of proteins using EPR spectroscopy. Methanethiosulfonate (MTS) spin labels tethered through a disulfide linkage to an engineered cysteine residue were used in a large number of studies to extract structural as well as dynamic information on the protein from the rotational dynamics of the nitroxide moiety. The ring itself was always considered to be a rigid body. In this contribution, we present a combination of high-resolution X-ray crystallography and EPR spectroscopy of spin-labeled protein single crystals demonstrating that the nitroxide ring inverts fast at ambient temperature while exhibiting nonplanar conformations at low temperature. We have used quantum chemical calculations to explore the potential energy that determines the ring dynamics as well as the impact of the geometry on the magnetic parameters probed by EPR spectroscopy

Inhibition of the crosstalk between dendritic, natural killer and T cells by mesenchymal stromal/stem cells

Mesenchymale Stromazellen (MSC) unterstützen die endogene Geweberegeneration und sind kaum immunogen. Die Mechanismen der Immunmodulation sind kaum bekannt. Diese Arbeit untersucht, ob MSC in die Interaktion von Dendritischen Zellen (DC), Natürlichen Killer (NK) und T Zellen eingreifen, indem sie die DC-Reifung beeinflussen. Das Netzwerk ist wichtig für die Differenzierung naïver T Zellen zu Typ 1 T Helferzellen (Th1). Abhängig vom DC-Subtyp und dem Zeitpunkt des Aufeinandertreffens, beeinflussten Knochenmark-MSC (BM-MSC) die in vitro DC-Reifung verschieden. Sie inhibierten die Differenzierung, aber nicht die Reifung humaner von Monozyten-abgeleiteter DC (moDC). BM MSC hatten keinen klaren Einfluss auf die Reifung plasmazytoider DC (pDC), während sie in aktivierten CD1c+ myeloiden DC (mDC) einen tolerogenen Phänotyp induzierten, charakterisiert durch eine geringere CCR7-abhängige Migration und ein tolerogenes Zytokinprofil. Daraus resultierend, wiesen BM-MSC-geprägte mDC aufgrund der veränderten IL 12/IL 10 Sekretion eine geringere Fähigkeit zur Stimulation der IFNγ Produktion in NK Zellen auf und induzierten weniger Th1 Differenzierung naïver T Zellen. Placenta-derived mesenchymal-like adherent stromal cells (PLX PAD) erzielten ähnliche Ergebnisse. Es konnte keine Alloimmunogenität in Patienten mit kritischer Ischämie der Extremitäten (CLI), die im Rahmen einer Phase I klinischen Studie allogene PLX PAD erhalten hatten, nachgewiesen werden. Keiner der Patienten entwickelte eine signifikante Gedächtnis T Zellantwort spezifisch für das Zellprodukt, was durch unsere in vitro Beobachtungen erklärbar sein könnte. Es ist schwierig MSC im Gewebe nachzuweisen, da Markerkombinationen notwendig sind. CD73+CD90+CD105+CD45-CD34-CD14-CD19- MSC konnten mithilfe einer neuen Multiplex-Immunhistologie-Technik (Chipzytometrie) in humanen Plazentaschnitten detektiert werden. Für die Zukunft könnte damit die Interaktion injizierter MSC mit Immunzellen in Biopsien untersucht werden.Mesenchymal stromal cells (MSC) support endogenous tissue regeneration and seem to be low immunogenic, allowing application across MHC barriers. But little is known about the mechanisms for their immunomodulation. Hence, the main goal of this study was to understand if MSC interfere with the crosstalk between dendritic cells (DC), natural killer (NK) and T cells by influencing DC maturation. This network is important for efficient priming of naïve T cells into type 1 helper T cells (Th1). Bone marrow-derived MSC (BM-MSC) had diverse effects on DC maturation in vitro, depending on the DC subset and the time of interaction. BM MSC inhibited differentiation but not maturation of monocyte-derived DC (moDC). They did not have a clear effect on maturation of plasmacytoid DC (pDC), whereas they induced a tolerogenic phenotype in activated CD1c+ myeloid DC (mDC), characterized by an impaired CCR7-dependent migration and a tolerogenic cytokine profile. Consequently, BM-MSC-licensed mDC displayed a reduced ability to induce IFNγ production in NK cells due to their altered IL 12/IL 10 secretion. BM MSC-licensed mDC also induced less efficiently Th1 lineage commitment of naïve T cells. Similar results were observed with placenta-derived mesenchymal-like adherent stromal cells (PLX PAD). Samples from critical limb ischemia (CLI) patients treated with MHC-unmatched PLX-PAD within a phase I clinical trial were analysed for alloimmunogenicity. None of the patients developed a significant memory T cell response specific to the allogeneic cells, which might be explainable by our in vitro observations. MSC are difficult to detect in tissues because a set of lineage markers is needed. Here, CD73+CD90+CD105+CD45-CD34-CD14-CD19- MSC could be identified in human placenta cryosections using a novel multiplex-immunohistology technique (chipcytometry), offering the possibility to investigate the crosstalk between injected MSC and attracted immune cells in patient biopsies in the future

De barbarismis et metaplasmis / digital edition published by digilibLT digital library of late-Latin texts

transcodifica di base da rtf a xml-tei piero colombo - step srl Testo Marinone, GL-CD Controllo marcatura XML-TEI Beatrice Bersani HomePage del progetto: https://digiliblt.uniupo.it/ Documentazione: https://digiliblt.uniupo.it/progetto.ph

De nomine et uerbo / digital edition published by digilibLT digital library of late-Latin texts

transcodifica di base da rtf a xml-tei piero colombo - step srl Testo Marinone, GL-CD Controllo marcatura XML-TEI Beatrice Bersani HomePage del progetto: https://digiliblt.uniupo.it/ Documentazione: https://digiliblt.uniupo.it/progetto.ph

Alt-Berlin

ALT-BERLIN Alt-Berlin / Consentius, Ernst (Public Domain) ( - ) Cover front ( - ) Die Königl. Preuss. Residentz Berlin nach ihrem accuraten Grundriss u. zweien Prospecten, auch Abbildung der sämtl. Kirchen und vornehmsten Königl. Gebäuden derselben / Schleuen, Johann David ( - ) Illustration: Solenne Erbhuldigung Sr. Königl. Maj. zu Berlin, d. 3. Aug. 1740 ; Durch Eröffnung der Königl. Magazine wird dem überhand nehmenden Brotmangel abgeholfen, im Jun. 1740 ( - ) Title page ( - ) Contents ( - ) Illustration: Prospect des Königl. Schlosses zu Berlin, wie sich selbiges gegen die Breite- und Brüder-Strasse zu praesentiret ( - ) Einleitung ([1]) Friedrichstadt und Bauspekulation ([12]) Illustration: Prospect des Königl. Schlosses zu Berlin, wie dasselbe gegen den Parade-Platz, und der Schloss-Freiheit sich praesentiret (Ill.: Schleuen, Johann David) ( - ) Illustration: Prospect des Berlinischen Rathhauses, wie solches A. in der Spandauer Strasse, und B. in der Königs Strasse anzusehen ( - ) Altstadt und Vorstädte ([41]) Illustration: Prospect des Cron-Prinzl. Palais zu Berlin ( - ) Die Wohnung ([75]) Das Gesinde ([94]) Illustration: Prospect des Grossen Friedrichs-Hospitals und Waysen-Hauses zu Berlin. (Ill.: Schleuen, Johann David) ( - ) Illustration: Prospect des Ordens-Palais Sr. Königl. Hoheit des Prinzen Ferdinands zu Berlin ( - ) Vom Weine ([115]) Brot, Fleisch und Bier ([130]) Illustration: Prospect des Königl. Arsenals zu Berlin ( - ) Die Mode ([174]) Illustration: Prospect von Monbijoux, dem Königl. Lust-Schloss und Sommer-Palais der Königl. Frau Mutter, wie selbiges sich gegen die so genannte Chaussé praesentiret ( - ) Schluß ([208]) Illustration: Prospect des Königlichen Opern-Hauses zu Berlin (Ill.: Schleuen, Johann David) ( - ) Anmerkungen ([216]) Personen-Register ([269]) Sach-Register ([276]) Illustration: Prospect der Stadt Berlin, wie solche von der Friedrich-Stadt, vorm Hallischen Thor sich praesentiret (Ill.: Schleuen, Johann David) ( - ) Cover back ( - ) ColorChart ( -