Innovative Service-Based Business Concepts for the Machine Tool Building Industry

Organised by: Cranfield UniversityDuring the last decade, machine tool building companies have been forced to put innovative offers on the market. Due to the technical features of their products and the prevailing organizational structures in this sector, especially product-service systems are a promising way of creating a unique selling point. In this paper, potential new business concepts for machine tool builders will be presented which aim at fulfilling basic customer needs like the increase in quality, flexibility, productivity and the reduction of lead times, costs and risks. For the implementation of these product-service systems, practical examples are given.Mori Seiki – The Machine Tool Compan

Transcriptional regulation of metabotropic glutamate receptor 2/3 expression by the NF-κB pathway in primary dorsal root ganglia neurons: A possible mechanism for the analgesic effect of L-acetylcarnitine

L-acetylcarnitine (LAC), a drug utilized for the treatment of neuropathic pain in humans, has been shown to induce analgesia in rodents by up-regulating the expression of metabotropic glutamate receptor 2 (mGlu2) in dorsal root ganglia (DRG). We now report that LAC-induced upregulation of mGlu2 expression in DRG cultures involves transcriptional activation mediated by nuclear factor-kappaB (NF-κB). A single application of LAC (250 μM) to DRG cultures induced a transient increase in mGlu2 mRNA, which was observable after 1 hour and was no longer detectable after 1 to 4 days. In contrast, LAC treatment had no effect on mGlu3 mRNA expression. Pharmacological inhibition of NF-κB binding to DNA by caffeic acid phenethyl ester (CAPE) (2.5 μg/ml for 30 minutes) reduced the constitutive expression of mGlu2 and mGlu3 mRNA after 1–4 days and reduced the constitutive expression of mGlu2/3 protein at 4 days. This evidence combined with the expression of p65/RelA and c-Rel in DRG neurons indicated that expression of mGlu2 and mGlu3 is endogenously regulated by the NF-κB family of transcription factors. Consistent with this idea, the transient increase in mGlu2 mRNA induced by LAC after 1 hour was completely suppressed by CAPE. Furthermore, LAC induced an increase in the acetylation of p65/RelA, a process that enhances the transcriptional activity of p65/RelA. These results are consistent with the hypothesis that LAC selectively induces the expression of mGlu2 by acting as a donor of acetyl groups, thus enhancing the activity of the NF-κB family of transcription factors. Accordingly, we show that carnitine, which has no effect on pain thresholds, had no effect on p65/RelA acetylation and did not enhance mGlu2 expression. Taken together, these results demonstrate that expression of mGlu2 and mGlu3 mRNA is regulated by the NF-κB transcriptional machinery, and that agents that increase acetylation and activation of NF-κB transcription factors might induce analgesia via upregulation of mGlu2 in DRG neurons

Leguminosas bioactivas para mejorar el perfil lipídico de la carne de cordero

6 páginas, 2 figuras, 1 tabla.La utilización de leguminosas forrajeras en la dieta de los rumiantes puede ofrecer importantes ventajas. Además de su elevado valor proteico, algunas especies como la esparceta o el trébol rojo, contienen compuestos bioactivos que podrían mejorar la calidad nutricional de la carne. Entre ellos, destacan los taninos en la esparceta y la polifenol oxidasa en el trébol rojo. Ahora bien, la climatología de muchas áreas limita su aprovechamiento en verde. En este estudio, mostramos que la utilización de la esparceta y el trébol rojo conservados mediante ensilado podría ser una buena alternativa para la alimentación de corderos, al aumentar la concentración de ácidos grasos poliinsaturados (PUFA) en la carne a través de su efecto sobre el metabolismo lipídico ruminal. Aunque el mecanismo de acción de los compuestos bioactivos presentes en estos forrajes probablemente sea distinto, sus efectos sobre el perfil de ácidos grasos de la carne parecen ser similares y aditivos.Este trabajo forma parte del proyecto Legume Plus, financiado por la Comisión Europea a través de una Red de Formación Inicial Marie Curie (PITN GA- 2011- 289377) PG . Toral disfruta de un contrato Ramón y Cajal, financiado por el Ministerio de Economía, lndustria y Competitividad.Peer reviewe

Sodium hydroxide enhances extractability and analysis of proanthocyanidins in ensiled sainfoin (onobrychis viciifolia)

Little information exists on the effects of ensiling on condensed tannins or proanthocyanidins. The acetone–butanol–HCl assay is suitable for measuring proanthocyanidin contents in a wide range of samples, silages included, but provides limited information on proanthocyanidin composition, which is of interest for deciphering the relationships between tannins and their bioactivities in terms of animal nutrition or health. Degradation with benzyl mercaptan (thiolysis) provides information on proanthocyanidin composition, but proanthocyanidins in several sainfoin silages have proved resistant to thiolysis. We now report that a pretreatment step with sodium hydroxide prior to thiolysis was needed to enable their analysis. This alkaline treatment increased their extractability from ensiled sainfoin and facilitated especially the release of larger proanthocyanidins. Ensiling reduced assayable proanthocyanidins by 29%, but the composition of the remaining proanthocyanidins in silage resembled that of the fresh plants

Hyperalgesic activity of kisspeptin in mice.

BACKGROUND: Kisspeptin is a neuropeptide known for its role in the hypothalamic regulation of the reproductive axis. Following the recent description of kisspeptin and its 7-TM receptor, GPR54, in the dorsal root ganglia and dorsal horns of the spinal cord, we examined the role of kisspeptin in the regulation of pain sensitivity in mice. RESULTS: Immunofluorescent staining in the mouse skin showed the presence of GPR54 receptors in PGP9.5-positive sensory fibers. Intraplantar injection of kisspeptin (1 or 3 nmol/5 μl) induced a small nocifensive response in naive mice, and lowered thermal pain threshold in the hot plate test. Both intraplantar and intrathecal (0.5 or 1 nmol/3 μl) injection of kisspeptin caused hyperalgesia in the first and second phases of the formalin test, whereas the GPR54 antagonist, p234 (0.1 or 1 nmol), caused a robust analgesia. Intraplantar injection of kisspeptin combined with formalin enhanced TRPV1 phosphorylation at Ser800 at the injection site, and increased ERK1/2 phosphorylation in the ipsilateral dorsal horn as compared to naive mice and mice treated with formalin alone. CONCLUSION: These data demonstrate for the first time that kisspeptin regulates pain sensitivity in rodents and suggest that peripheral GPR54 receptors could be targeted by novel drugs in the treatment of inflammatory pain.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Produzione, qualità e analisi della filiera produttiva del lino da fibra in Italia

Fibre flax (Linum usitatissimum L.) is disappeared from the ltalian crop systems, while the textile industry has reached a leading position. The reintroduction of the crop is than potentially promising but the crop techniques and the production processes have to be set up. In 12 environments: crop development and growth, cultivar response, straw, fibre and seed yields, and fibre quality were analysed in the 1992-98 period. The data pointed out crop cycles of 176 and 110 d for the autumn and the spring sowing time respectively, corresponding to 1125 and 990°C d cumulated growing degree. Straw yield at pulling was on average of 6.3 t ha-1, with relevant differences among years and environrnents. Early maturity cultivar yielded more in the less favourable sites. N fertilization was occasionally relevant, and a generally distributions of 60 kg N ha-1 was the more effective. In the Mediterranean environments, with the autumn, sowing flax was more productive and the yield steady. The long fibre ratio was often low (10-13%) and the quality uneven because of the insufficient cleanliness due to retting difficulties. Occasionally, the value of the fibre was affected by the insufficient stem length. In the less dense crop, the seed yield was on average 0.9 t ha-1. Such productions were similar to those assessed for the more dense crop. In conclusion: because of the complexity of the interactions among the crop growth, the yield, the retting process and the fibre quality flax reintroduction is doubtful. In the production processes retting seems to be the crucial phase. Il lino da fibra (Linum usitatissimum L.) è da tempo assente negli ordinamenti colturali italiani, mentre è assai importante l'industria di filatura. La reintroduzione della coltura appare quindi potenzialmente promettente, ma richiede la messa a punto dell'agrotecnica e della filiera produttiva. Nel periodo 1992-1998 in 12 ambienti sono stati esaminati: lo sviluppo e la crescita della coltura, l'adattamento di cultivar, la produzione di paglia, fibra e seme, la resa alla stigliatura, la qualità della fibra. I dati raccolti evidenziano cicli colturali in media di 176 e 110 d rispettivamente per le semine autunnali (ambienti del centro-sud) e vemino-primaverili; corrispondenti a somme termiche di 1125 e 990°C d. La produzione di paglia alla estirpatura è stata in media di 6.3 t ha-1 con valori assai variabili tra annate e ambienti. Le varietà a ciclo corto sono apparse migliori nelle condizioni difficili. L'effetto della concimazione azotata è apparsa talvolta rilevante e comunque con dosi ottimali prossime a 60 kg ha-1 di N. Negli ambienti del centro-sud le semine autunnali si sono dimostrate spesso le più interessanti e sicure. La resa in fibra lunga è stata bassa (10-13%) e la qualità variabile per la scarsa pulizia dovuta alle difficoltà di macerazione. La modesta lunghezza tecnica dello stelo sovente riduce il valore del prodotto. In coltura rada, la produzione di seme è variata da 0.5 a 1.5 t ha-1; tali valori non si sono discostati in modo apprezzabile da quelli ottenuti in coltura fitta. In conclusione, la diffusione del lino appare problematica per le complesse interazioni tra la produzione, il processo di macerazione e la qualità della fibra. Soprattutto la macerazione appare il passaggio chiave

Las Marías and the dictatorship : business responsibility in labour repression during state terrorism in a Northeastern Argentine company

Fil: Copani, Andrea. Universidad Nacional de Buenos Aires. Facultad de Filosofía y Letras. Instituto Interdisciplinario de Estudios e Investigaciones de América Latina; Argentina.En este artículo pretendemos sintetizar los hallazgos presentados en el informe Responsabilidad empresarial en delitos de lesa humanidad. Represión a trabajadores durante el terrorismo de Estado relativos al caso de la empresa yerbatera correntina Las Marías. Nos centraremos en la reconstrucción de la represión sufrida por los trabajadores de la firma a partir del golpe de Estado del 24 de marzo de 1976, que ubicaremos en el contexto del diseño y despliegue represivo a nivel regional. A su vez, identificaremos aquellas prácticas empresariales represivas que surgen de la evidencia disponible hasta el momento. Por otra parte, abordaremos los principales hitos del proceso de denuncia, visibilización pública y judicialización del caso, desde sus inicios hasta la actualidad, atendiendo tanto a sus particularidades como a los rasgos que comparte con otros casos de delitos de lesa humanidad cometidos contra trabajadores.In this article, we aim at summarising the conclusions presented in the report called Responsabilidad empresarial en delitos de lesa humanidad. Represión a trabajadores durante el terrorismo de Estado (Business Responsibility in Crimes against Humanity. Labour Repression during State Terrorism) related to the case of Las Marías yerba grower’s in the Province of Corrientes. We will focus on the reconstruction of the repression suff ered by the fi rm’s workers from the coup d’état on March 24th, 1976, which we will be placing in the context of a repressive design and deployment at a regional level. Likewise, we will identify those repressive business practices which emerge from the evidence which has been available so far. On the other hand, we will deal with the main milestones of the denouncement process, public visibility and case legal proceedings, from its origin to the present time, paying attention to its particularities as well as to those features shared with other cases of crimes against humanity committed against workers

Assessing the accuracy of current near infra-red reflectance spectroscopy analysis for fresh grass-clover mixture silages and development of new equations for this purpose

The purpose of this study was to ascertain whether Near Infra-Red Reflectance Spectroscopy (NIRS) prediction equations calibrated on grass silage samples, could accurately predict the chemical composition of mixed grass-clover silage samples, and furthermore, to develop and calibrate new grass-clover equations should the grass-based equations be insufficiently accurate for these silages. A set of 94 silage samples from mixed grass-clover swards (clover concentration (CC) ranging from 4 to 1000 g/kg as fed; determined manually) were analysed for chemical composition using reference laboratory techniques, in vivo digestible organic matter in the dry matter (DOMD, in sheep), and in situ degradability of dry matter and crude protein (in cows). The same samples were scanned fresh (undried and unmilled, as is standard practice for silage analysis within UK laboratories) using NIRS (at AFBI, Northern Ireland) and grass-based prediction equations applied. Predicted and observed results were compared. Of 15 chemical components that were tested for prediction accuracy, only volatile-corrected dry matter and nitrogen were well predicted (RPD values of 4.9 and 2.4 respectively, with low root mean square errors of prediction (RMSEP)). Neutral detergent fibre and DOMD showed low RPD values, however the predicted and observed datasets had no significant bias between them and were therefore also considered as fit for purpose. Variables with significant bias between predicted and observed datasets that were not considered suitably accurate included crude protein, acid detergent fibre, microbial dry matter yield and the effective degradability of protein. For many components, bias could be attributed at least in part to CC and changes in the fractionation of nutrients present. For some variables such as crude protein, grass-based equations were sufficiently accurate at low CCs but became inaccurate as CC increased, as expected. In response to inadequate prediction accuracy of certain nutrients, new grass-clover equations were calibrated using the obtained spectra. These were validated and results indicated that the grass-clover-based equations outperformed their grass-based counterparts. The adoption of new grass-clover equations, or alternatively, with further development, the use of a CC correction factor to the existing grass-based equations, is recommended for commercial laboratories offering undried and unmilled silage analysis on samples containing clover

2. Greci e indigeni ad Eloro

L’histoire de Syracuse dans la phase la plus archaïque est bien mal connue. La tradition d’un conflit entre les Grecs et les indigènes (Thucydide) n’exclut pas une première période de cohabitation. Les rapports avec les indigènes ne sont pas clairs au moins jusqu’à la destruction du village de Monte Finocchito. Dans ce cadre devient très importante l’interprétation de la fondation de la colonie syracusaine d’Héloros (fin VIIIe sec. a.C.). Elle a été souvent considérée comme colonie militaire et comme barrière contre les Sicules, mais peut-être eut- elle un rôle différent, celui d’occuper un site qui pouvait être facilement choisi par d’autres Grecs comme siège d’une nouvelle colonie. Dans cette appropriation de la colline d’Héloros, les Syracusains se servirent d’un groupe d’indigènes. Finalement il faut reconsiderer l’idée que les indigènes representèrent un danger réel et constant pour Syracuse à l’époque archaïque