Cuando la relación materno-filial termina en los tribunales. Pleitos de doña Antonia de la Cerda, marquesa consorte de Aguilar de Campoo

Con este trabajo se pretende dar a conocer la conflictividad judicial a la que tuvo que hacer frente la marquesa consorte de Aguilar de Campoo, doña Antonia de la Cerda cuando, debido a la minoría de edad de su hijo, heredero del mayorazgo, hubo de hacerse cargo de la dirección de la Casa nobiliaria de su marido. Además, también se analizan los pleitos habidos entre madre e hijo en el marco general de una casa nobiliaria endeudada y en concurso de acreedores.Trabajo vinculado a las actividades del proyecto de investigación: “Justicia y mujer. Los tribunales penales en la definición de una identidad de género. Castilla y Portugal (1550-1800)”, con referencia HAR2012-31909 de los Proyectos de Investigación Fundamental. VI Programa Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica

Mujeres parleras y desvergonzadas. Del insulto callejero al delito de injurias

The present study is aimed at bringing to light an aspect of feminine sociability based on conflict. Insults – in this case, among women– led to a decrease in the honour and reputation of the slandered person and his or her family. Therefore, when facing this charge, both Castilian legislation and courts of justice leant toward a type of conciliatory sentences, aware that excessive harshness could result in a greater damage within the patterns of proximity and coexistence in the society of the Ancien Régime.Este trabajo forma parte del proyecto de investigación: Proyecto “Justicia y mujer. Los tribunales penales en la definición de una identidad de género. Castilla y Portugal (1550-1800)”, HAR2012-31909, financiado por el Ministerio de Economía y Competitividad. Proyectos de Investigación Fundamental. VI Programa Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, 2008-201

Distribution and interaction between selected pharmaceuticals and hydrogeochemistry in Guadalhorce aquifer(South of Spain)

Management of water resources implies the study of their quality and in recent times one of the main drawbacks is the presence of non-regulated products, the so-called "Emerging Contaminants (EC)". Currently the study of these contaminants is among the research priorities of the main organizations dedicated to the protection of public and environmental, such as OMS, EPA or the EU Commission health. The list of emerging contaminants include a wide variety of products both industrial and domestic applications, whose harmful effects include toxicity, bioaccumulation, and even endocrine disruption. Conventional wastewater treatment technologies are not efficient at removing many of these contaminants, so effluents and reuse become one of the most significant sources of contamination, even many emerging pollutants are only partially degraded and are even more toxic byproducts than the parent compounds. This research is focused in the Guadalhorce porous aquifer (Malaga, south of Spain), to study the distribution of EC and the interaction with the hydrogeochemistry. 14 EC (Ibuprofen, Hydrochlorothiazide, Salicilic Acid, Triclosan, Menfenamic Acid, Carbamazepine, Trimethoprim, Ofloxacin, Cafeine, Metotrexato, Metronidazole, Sulfadiazine, Sulfamethazine and Sulfamethoxazole) and 36 paramaters including electrical conductivity, T, pH, DO, major ions (Cl-, SO42-, HCO3-, NO3-, Ca2+, Mg2+, Na+, K+), metals (B, Al, Fe, Mn, Cd, Pb, Zn, As and Se) and isotopes (d2H-H2O, d18O-H2O, d34S-SO42-) were controlled in a single field sampling (June 2012). Relationships between certain pollutants by its focus of origin are studied. Ibuprofen and Menfenamic acid follow the same pattern since they have the same use as anti-inflammatory agents and origin, mainly related to urban centers, which is important in the Guadalhorce area close to the mouth. Some possible explanations are the close situation of the Waste Water Treatment Plant of Malaga, numerous discharges of wastewater, irrigations with reused and non-reused water, among others. Nearby to the upper part of the aquifer, where farms and livestock are concentrated, pharmaceuticals for veterinary use are frequent. Furthermore, a relationship between certain emerging contaminants and hydrogeochemical characteristics present in the groundwater masses is observed, noting that ibuprofen, for example, has an indirect correlation to the temperature and concentration of certain ions such as chlorine and lead present in water masses. So it may be said that the distribution and concentration of emerging contaminants is not only affected by the number of pollution sources but also by the hydrogeochemical characteristics of the aquifer.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Estudio de contaminantes emergentes en acuíferos detríticos de la cuenca hidrográfica del Río Guadalhorce (Málaga)

Actualmente existe un creciente interés por los contaminantes emergentes, en especial por los productos farmacéuticos y de cuidado personal (PPCPs), ya que son contaminantes que han pasado inadvertidos a lo largo de los años debido a sus bajas concentraciones, pero que presentan efectos perjudiciales para la salud y el medio ambiente, algunos de ellos se comportan, incluso, como disruptores endocrinos. Estos compuestos, en su mayoría, no regulados en la legislación, han sido hallados en aguas superficiales, subterráneas, sedimentos e incluso en aguas de abastecimiento. Este trabajo se centra en las aguas subterráneas de la cuenca del río Guadalhorce, determinando la distribución de los PPCPs, y la relación que presentan con las fuentes de contaminación existentes en el área de estudio.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Wnt/beta-catenin signaling controls development of the blood–brain barrier

The blood–brain barrier (BBB) is confined to the endothelium of brain capillaries and is indispensable for fluid homeostasis and neuronal function. In this study, we show that endothelial Wnt/beta-catenin (beta-cat) signaling regulates induction and maintenance of BBB characteristics during embryonic and postnatal development. Endothelial specific stabilization of beta-cat in vivo enhances barrier maturation, whereas inactivation of beta-cat causes significant down-regulation of claudin3 (Cldn3), up-regulation of plamalemma vesicle-associated protein, and BBB breakdown. Stabilization of beta-cat in primary brain endothelial cells (ECs) in vitro by N-terminal truncation or Wnt3a treatment increases Cldn3 expression, BBB-type tight junction formation, and a BBB characteristic gene signature. Loss of beta-cat or inhibition of its signaling abrogates this effect. Furthermore, stabilization of beta-cat also increased Cldn3 and barrier properties in nonbrain-derived ECs. These findings may open new therapeutic avenues to modulate endothelial barrier function and to limit the devastating effects of BBB breakdown

Применение метода долгосрочного прогнозирования водонефтяного фактора для определения максимально возможного расчётного объёма добычи нефти месторождения "Чёрный Дракон", Вьетнам

Objective - Although junctional adhesion molecule-A (JAM-A) has recently been implicated in leukocyte recruitment on early atherosclerotic endothelium and after reperfusion injury, its role in neointima formation after arterial injury remains to be elucidated. Methods and Results - Here we show that the genetic deletion of JAM-A in apolipoprotein E - deficient (apoE(-/-)) mice significantly reduced neointimal hyperplasia after wire injury of carotid arteries without altering medial area. This was associated with a significant decrease in neointimal macrophage content, whereas the relative content of smooth muscle cells and endothelial recovery was unaltered in JAM-A(-/-) apoE(-/-) compared with JAM-A(-/-) apoE(-/-) lesions. In carotid arteries perfused ex vivo, deficiency in JAM-A significantly impaired the recruitment of monocytes 1 week, but not 1 day, after injury. These effects were paralleled by an attenuation of monocyte arrest and transmigration on activated JAM-A(-/-) apoE(-/-) versus JAM-A(-/-) apoE(-/-) endothelial cells under flow conditions in vitro. A mechanism underlying reduced recruitment was implied by findings that the luminal expression of the arrest chemokine RANTES in injured arteries and its endothelial deposition by activated platelets in vitro were diminished by JAM-A deficiency. Conclusions - Our data provide the first evidence to our knowledge for a crucial role of JAM-A in accelerated lesion formation and monocyte infiltration in atherosclerosis-prone mice

Angiopoietin receptor Tie2 is required for vein specification and maintenance via regulating COUP-TFII

Mechanisms underlying the vein development remain largely unknown. Tie2 signaling mediates endothelial cell (EC) survival and vascular maturation and its activating mutations are linked to venous malformations. Here we show that vein formation are disrupted in mouse skin and mesentery when Tie2 signals are diminished by targeted deletion of Tek either ubiquitously or specifically in embryonic ECs. Postnatal Tie2 attenuation resulted in the degeneration of newly formed veins followed by the formation of haemangioma-like vascular tufts in retina and venous tortuosity. Mechanistically, Tie2 insufficiency compromised venous EC identity, as indicated by a significant decrease of COUP-TFII protein level, a key regulator in venogenesis. Consistently, angiopoietin-1 stimulation increased COUP-TFII in cultured ECs, while Tie2 knockdown or blockade of Tie2 downstream PI3K/Akt pathway reduced COUP-TFII which could be reverted by the proteasome inhibition. Together, our results imply that Tie2 is essential for venous specification and maintenance via Akt mediated stabilization of COUP-TFII.Peer reviewe

Climacteric Lowers Plasma Levels of Platelet-Derived Microparticles: A Pilot Study in Pre-versus Postmenopausal Women

Background: Climacteric increases the risk of thrombotic events by alteration of plasmatic coagulation. Up to now, less is known about changes in platelet-(PMP) and endothelial cell-derived microparticles (EMP). Methods: In this prospective study, plasma levels of microparticles (MP) were compared in 21 premenopausal and 19 postmenopausal women. Results: No altered numbers of total MP or EMP were measured within the study groups. However, the plasma values of CD61-exposing MP from platelets/megakaryocytes were higher in premenopausal women (5,364 x 10(6)/l, range 4,384-17,167) as compared to postmenopausal women (3,808 x 10(6)/l, range 2,009-8,850; p = 0.020). This differentiation was also significant for the subgroup of premenopausal women without hormonal contraceptives (5,364 x 10(6)/l, range 4,223-15,916; p = 0.047; n = 15). Furthermore, in premenopausal women, higher plasma levels of PMP exposing CD62P were also present as compared to postmenopausal women (288 x 10(6)/l, range 139-462, vs. 121 x 10(6)/l, range 74-284; p = 0.024). This difference was also true for CD63+ PMP levels (281 x 10(6)/l, range 182-551, vs. 137 x 10(6)/l, range 64-432; p = 0.015). Conclusion: Climacteric lowers the level of PMP but has no impact on the number of EMP in women. These data suggest that PMP and EMP do not play a significant role in enhancing the risk of thrombotic events in healthy, postmenopausal women. Copyright (C) 2012 S. Karger AG, Base

Towards a holistic understanding of the role of green infrastructure in improving urban air quality

Air pollution has been identified as a major problem in modern societies, threatening urban population health. Pedestrians, in particular, are directly exposed to one of the main sources of air pollutants: road transport, which is concentrated in proximity to the road, worsening the air. Green infrastructure (GI) has been promoted as a natural method for reducing exposure to local street air pollutants and providing additional Ecosystem Services with a range of environmental, social and economic benefits for citizens. The effectiveness of GI for improving air quality depends on the spatio-temporal context and the species-specific characteristics of the GI. Urban planting could maximise this benefit by a holistic understanding of the effects of GI in cities, balancing its benefits and constraints. However, little is currently known about the application of GI design and planning with regard to air pollution mitigation. Moreover, there is little agreement on the quantifiable effectiveness of GI in improving street air quality as its effectiveness is highly context dependent. Holistic guidance is therefore needed to inform practitioners of site- and species- specifics, trade-offs, and GI maintenance considerations for successful urban planting. This research reviews the academic literature addressing GI-related characteristics in streets, creating a holistic framework to help guide decision-makers on using GI solutions to improve air quality. Additionally, this research aims to understand how and which GI, along with other local characteristics, influence pedestrian air quality and how these characteristics are considered in real-world practice within the United Kingdom. This research progresses through three stages: First, the mechanisms by which GI is considered to influence air quality were identified through literature reviews. A specific literature review was then conducted for each mechanism to extract the associated GI and spatial characteristics that affect the potential for GI to mitigate urban air pollution. In the second stage, this list of characteristics, together with other Ecosystem Services, was discussed in consultation with practitioners in the UK. A survey was conducted to explore and evaluate the recommendations and resources available for planning plantings, as well as the practitioners’ knowledge about the characteristics associated with mitigating air pollution. Supported by results from the survey and the literature reviews, the third stage evaluated (validated) an easy-to-use computational model for its potential use in improving planting decisions for air pollution mitigation. Green infrastructure influences air quality by providing surfaces for pollutant deposition and absorption, effects on airflow and dispersion, and biogenic emissions. The relationship between the specific GI and the spatio-temporal context also influences air quality. Street structure, weather variables, and the type, shape and size of GI influence the dispersion of pollutants, with micro-and macro-morphological traits additionally influencing particulate deposition and gas absorption. In addition, maintaining GI lessens air quality deterioration by controlling biogenic emissions. According to participants in the survey, aesthetics were the principal drivers of urban planting, followed by improving well-being and increasing biodiversity and air pollution mitigation as a lesser priority. Characteristics such as airflow manipulation, leaf surface traits, and biogenic emissions were the less important influences in planting decisions in the UK, despite the fact that these characteristics influence air quality. Perhaps, a lack of communication of current information and low confidence about which specific characteristics have a tangible effect on air quality reduces the incorporation of GI for air pollution mitigation purposes. Uncertainties exist about the quantification of pollutants removed by GI. Field campaigns and computational models still need improvement to address the effectiveness of GI in real-world environments adequately and also to understand whether GI can exert a significant effect on pollutant levels under real-world conditions. This research showed that a promising and easy-to-use model used to evaluate the effectiveness of trees in removing particles was not an acceptable model to study the effect of GI on streets. The validation results showed a poor agreement between wind tunnel data and the model results. More effort is needed to develop better modelling tools that can quantify the actual effect of GI on improving street air quality. This research contributes to the air pollution mitigation field, explicitly helping to inform decision-making for more health-promoting urban settings by optimising the expected benefits of GI through a holistic understanding of their impacts. Facilitating the communication of current evidence through a holistic guide that considers both the benefits and trade-offs of planting decisions for air quality improvement. Improving information on air pollution mitigation to feed the decision-making process might maximise the benefits of GI planting for air pollution mitigation in streets.Open Acces

Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression

Endothelial Wnt/β-catenin signaling is necessary for angiogenesis of the central nervous system and blood–brain barrier (BBB) differentiation, but its relevance for glioma vascularization is unknown. In this study, we show that doxycycline-dependent Wnt1 expression in subcutaneous and intracranial mouse glioma models induced endothelial Wnt/β-catenin signaling and led to diminished tumor growth, reduced vascular density, and normalized vessels with increased mural cell attachment. These findings were corroborated in GL261 glioma cells intracranially transplanted in mice expressing dominant-active β-catenin specifically in the endothelium. Enforced endothelial β-catenin signaling restored BBB characteristics, whereas inhibition by Dkk1 (Dickkopf-1) had opposing effects. By overactivating the Wnt pathway, we induced the Wnt/β-catenin–Dll4/Notch signaling cascade in tumor endothelia, blocking an angiogenic and favoring a quiescent vascular phenotype, indicated by induction of stalk cell genes. We show that β-catenin transcriptional activity directly regulated endothelial expression of platelet-derived growth factor B (PDGF-B), leading to mural cell recruitment thereby contributing to vascular quiescence and barrier function. We propose that reinforced Wnt/β-catenin signaling leads to inhibition of angiogenesis with normalized and less permeable vessels, which might prove to be a valuable therapeutic target for antiangiogenic and edema glioma therapy